Tricyclic amine compounds as cdk2 inhibitors

ABSTRACT

The present application provides tricyclic amine compounds, which are inhibitors of cyclin-dependent kinase 2 (CDK2), as well as pharmaceutical compositions thereof, and methods of treating cancer using the same.

This application claims the benefit of priority of U.S. Prov. Appl. No.62/841,669, filed May 1, 2019, which is incorporated herein by referencein its entirety.

SEQUENCE LISTING

The instant application contains a Sequence Listing which has been filedelectronically in ASCII format and is hereby incorporated by referencein its entirety. Said ASCII copy, created on Apr. 23, 2020, is named20443-0606001_SL.txt and is 15.3 kilobytes in size.

TECHNICAL FIELD

This application is directed to tricyclic amine compounds which inhibitcyclin-dependent kinase 2 (CDK2) and are useful for treating cancer.

BACKGROUND

Cyclin-dependent kinases (CDKs) are a family of serine/threoninekinases. Heterodimerized with regulatory subunits known as cyclins, CDKsbecome fully activated and regulate key cellular processes includingcell cycle progression and cell division (Morgan, D. O., Annu Rev CellDev Biol, 1997, 13:261-91). Uncontrolled proliferation is a hallmark ofcancer cells. The deregulation of the CDK activity is associated withabnormal regulation of cell-cycle, and is detected in virtually allforms of human cancers (Sherr, C. J., Science, 1996, 274(5293):1672-7).

CDK2 is of particular interest because deregulation of CDK2 activityoccurs frequently in a variety of human cancers. CDK2 plays a crucialrole in promoting G1/S transition and S phase progression. In complexwith cyclin E (CCNE), CDK2 phosphorylates retinoblastoma pocket proteinfamily members (p107, p130, pRb), leading to de-repression ofE2Ftranscription factors, expression of G1/S transition related genes andtransition from G1 to S phase (Henley, S. A. and F. A. Dick, Cell Div,2012, 7(1):10). This in turn enables activation of CDK2/cyclin A, whichphosphorylates endogenous substrates that permit DNA synthesis,replication and centrosome duplication (Ekholm, S. V. and S. I. Reed,Curr Opin Cell Biol, 2000, 12(6):676-84). It has been reported that theCDK2 pathway influences tumorigenesis mainly through amplificationand/or overexpression of CCNE1 and mutations that inactivate CDK2endogenous inhibitors (e.g., p27), respectively (Xu, X., et al.,Biochemistry, 1999, 38(27):8713-22).

CCNE1 copy-number gain and overexpression have been identified inovarian, gastric, endometrial, breast and other cancers and beenassociated with poor outcomes in these tumors (Keyomarsi, K., et al., NEngl J Med, 2002, 347(20):1566-75; Nakayama, N., et al., Cancer, 2010,116(11):2621-34; Au-Yeung, G., et al., Clin Cancer Res, 2017,23(7):1862-1874; Rosen, D. G., et al., Cancer, 2006, 106(9):1925-32).Amplification and/or overexpression of CCNE1 also reportedly contributeto trastuzumab resistance in HER2+ breast cancer and resistance toCDK4/6 inhibitors in estrogen receptor-positive breast cancer(Scaltriti, M., et al., Proc Natl Acad Sci USA, 2011, 108(9):3761-6;Herrera-Abreu, M. T., et al., Cancer Res, 2016, 76(8):2301-13). Variousapproaches targeting CDK2 have been shown to induce cell cycle arrestand tumor growth inhibition (Chen, Y N., et al., Proc Natl Acad Sci USA,1999, 96(8):4325-9; Mendoza, N., et al., Cancer Res, 2003,63(5):1020-4). Inhibition of CDK2 also reportedly restores sensitivityto trastuzumab treatment in resistant HER2+ breast tumors in apreclinical model (Scaltriti, supra).

These data provide a rationale for considering CDK2 as a potentialtarget for new drug development in cancer associated with deregulatedCDK2 activity. In the last decade there has been increasing interest inthe development of CDK selective inhibitors. Despite significantefforts, there are no approved agents targeting CDK2 to date (Cicenas,J., et al., Cancers (Basel), 2014, 6(4):2224-42). Therefore it remains aneed to discover CDK inhibitors having novel activity profiles, inparticular those targeting CDK2. This application is directed to thisneed and others.

SUMMARY

The present invention relates to, inter alia, compounds of Formula (I):

or a pharmaceutically acceptable salt thereof, wherein constituentmembers are defined herein.

The present invention further provides pharmaceutical compositionscomprising a compound of Formula (I), or a pharmaceutically acceptablesalt thereof, and a pharmaceutically acceptable carrier.

The present invention further provides methods of inhibiting CDK2,comprising contacting the CDK2 with a compound of Formula (I), or apharmaceutically acceptable salt thereof.

The present invention further provides methods of inhibiting CDK2 in apatient, comprising administering to the patient a compound of Formula(I), or a pharmaceutically acceptable salt thereof.

The present invention also provides methods of treating a disease ordisorder associated with CDK2 in a patient, comprising administering tothe patient a therapeutically effective amount of the compound describedherein, or a pharmaceutically acceptable salt thereof.

The present invention further provides methods of treating a humansubject having a disease or disorder associated with cyclin-dependentkinase 2 (CDK2), comprising administering to the human subject acompound described herein, or a pharmaceutically acceptable saltthereof, wherein the human subject has been previously determined to:(i) (a) have a nucleotide sequence encoding a p16 protein comprising theamino acid sequence of SEQ ID NO:1; and/or (b) have a cyclin dependentkinase inhibitor 2A (CDKN2A) gene lacking one or more inactivatingnucleic acid substitutions and/or deletions; (ii) (a) have anamplification of the cyclin E1 (CCNE1) gene; and/or (b) have anexpression level of CCNE1 in a biological sample obtained from the humansubject that is higher than a control expression level of CCNE1.

The present invention additionally provides methods of treating a humansubject having a disease or disorder associated with cyclin-dependentkinase 2 (CDK2), comprising: (i) identifying, in a biological sampleobtained from the human subject: (a) a nucleotide sequence encoding ap16 protein comprising the amino acid sequence of SEQ ID NO:1; and/or(b) a cyclin dependent kinase inhibitor 2A (CDKN2A) gene lacking one ormore inactivating nucleic acid substitutions; (ii) identifying, in abiological sample obtained from the human subject: (a) an amplificationof the cyclin E1 (CCNE1) gene; and/or (b) an expression level of CCNE1that is higher than a control expression level of CCNE1; and (iii)administering a compound described herein, or a pharmaceuticallyacceptable salt thereof, to the human subject.

The present invention also provides methods of evaluating the responseof a human subject having a disease or disorder associated withcyclin-dependent kinase 2 (CDK2) to a compound described herein, or apharmaceutically acceptable salt thereof, comprising: (a) administeringthe compound or the salt, to the human subject, wherein the humansubject has been previously determined to have an amplification of thecyclin E1 (CCNE1) gene and/or an expression level of CCNE1 that ishigher than a control expression level of CCNE1; (b) measuring, in abiological sample of obtained from the subject subsequent to theadministering of step (a), the level of retinoblastoma (Rb) proteinphosphorylation at the serine corresponding to amino acid position 780of SEQ ID NO:3, wherein a reduced level of Rb phosphorylation at theserine corresponding to amino acid position 780 of SEQ ID NO:3, ascompared to a control level of Rb phosphorylation at the serinecorresponding to amino acid position 780 of SEQ ID NO:3, is indicativethat the human subject responds to the compound or the salt.

The present invention further provides a compound of Formula (I), or apharmaceutically acceptable salt thereof, for use in any of the methodsdescribed herein.

The present invention further provides use of a compound of Formula (I),or a pharmaceutically acceptable salt thereof, for the preparation of amedicament for use in any of the methods described herein.

DESCRIPTION OF DRAWINGS

FIGS. 1A-1B: Characterization of ovarian and endometrial cell lines.FIG. 1A: Cell lines used for study included four cell lines with CCNE1amplification and three cell lines with no CCNE1 amplification. CCNE1amplification copy numbers are indicated. FIG. 1B: The expression ofCCNE1 was determined by Western blot in indicated cell lines. This blotshow cell lines with CCNE1 gain of function by copy number (CN>2)expressed higher levels of CCNE1 protein compared with cell lines withcopy neutral or loss of function of the gene (CN≤2). GAPDH was detectedas a loading control. Non-Amp, non-amplification; Amp, amplification.

FIGS. 2A-2B: siRNA mediated CDK2 knockdown inhibits proliferation inCCNE1 amplified cell lines. FIG. 2A: CCNE1 amplified Fu-ov1 (upper) andKLE (lower) cells were harvested and subjected to cell cycle analysis 72hours after transfection with either scrambled siRNAs (“Ctl”) or CDK2siRNAs. The cell cycle phase distribution was evaluated by FACS. Shownare representative images of three separate experiments. FIG. 2B: CDK2knockdown was confirmed by Western blot analysis after transfection withCDK2 siRNA. GAPDH was used as a loading control.

FIGS. 3A-3B: CDK2 knockdown does not inhibit proliferation in CCNE1Non-Amp lines. FIG. 3A: CCNE1 non-amplified COV504 and Igrov1 cells wereharvested and subjected to cell cycle analysis 72 hours aftertransfection with Ctl siRNAs and CDK2 siRNAs. The cell cycle phasedistribution was evaluated by FACS. Shown are representative images ofthree separate experiments. FIG. 3B: CDK2 knockdown was confirmed byWestern blot analysis after transfection with CDK2 siRNA. GAPDH was usedas a loading control.

FIG. 4: CDK2 knockdown by siRNA inhibits proliferation in CCNE1amplified, but not in CCNE1 non-amplified, human cancer cell lines.Percentage of cells at the S phase 3 days after transfection of CDK2siRNAs, relative to Ctl siRNA. The cell cycle phase distribution wasevaluated by FACS. Means represent three independent experiments in fourCCNE1 Amp cell lines and three Non-Amp lines.

FIG. 5: Palbociclib treatment induces dose-dependent inhibition ofproliferation in CCNE1 non-amplified, but not in amplified cell lines.Cell cycle analysis of CCNE1 non-amplified cell line COV504 (upper) andCCNE1 amplified OVCAR3 cells (lower) after Palbociclib treatment for 16hours. The cell cycle phase distribution was evaluated by FACS.

FIG. 6: Palbociclib treatment selectively inhibits proliferation inCCNE1 non-amplified cancer cell lines. Percentage of cells at the Sphase after 16 hours of Palbociclib with the indicated doses, relativeto DMSO.

FIGS. 7A-7B: CDK2 knockdown by siRNAs blocks RB phosphorylation at S780in CCNE1 amplified, but not in non-amplified ovarian cells. FIG. 7A:Four CCNE1 Amp cell lines, COV318, Fu-OV1, OVCAR3 and KLE cells, weretransfected with CDK2 siRNAs for 72 hours. FIG. 7B: Three CCNE1 Non-Ampcell lines, COV504, OV56 and Igrov1, were transfected with CDK2 siRNAsfor 72 hours. The total proteins were extracted from CDK2 siRNA or CtlsiRNA transfected cells and subjected to western blotting. GAPDH wasused as a loading control.

FIGS. 8A-8B: Palbociclib blocks RB phosphorylation at S780 in CCNE1non-amplified, but not in amplified ovarian cells. FIG. 8A: CCNE1 AmpOVCAR3 and COV318 cells were treated at various concentrations ofPalbociclib as indicated for 1 hour or 15 h. FIG. 8B: CCNE1 Non-AmpCOV504 and OV56 were treated at various concentrations of Palbociclib asindicated for 1 hour or 15 h. The total proteins were extracted fromthese Palbociclib or DMSO (controls) treated cells and subjected towestern blotting. p-RB, phosphorylated retinoblastoma protein. GAPDH wasused as a loading control.

FIGS. 9A-9B: CDK2 degradation by dTAG decreases RB phosphorylation at5780. FIG. 9A: Chemical structure of dTAG. FIG. 9B: CDK2-FKBP12(F36V)degradation by CDK2-dTAG treatment for 14 hours inhibited RBphosphorylation at S780 in CDK2 knockout OVCAR3 (right, Cas9+,CDK2-FKBP12(F36V)-HA+, CDK2-gRNA) cells, but not in OVCAR3 cells withendogenous CDK2 (left, Cas9+, CDK2-FKBP12(F36V)-HA+, Ctl-gRNA).

FIGS. 10A-10B: p-RB S780 HTRF cellular Assay for identification of CDK2inhibitors. FIG. 10A: IC₅₀ in CDK2 biochemical kinase activity assay.FIG. 10B: Concentration response analysis of reference compounds testedin the p-RB S780 HTRF cellular assay. HTRF, homogeneous time-resolvedfluorescence. IC₅₀ from HTRF cellular Assay correlates with IC₅₀ in CDK2enzymatic assay.

FIG. 11: Bioinformatics analysis of CCLE dataset reveals the sensitivityto CDK2 inhibition in CCNE1 amplified cells relies on functional p16.FIG. 11 shows the status of p16 in CDK2 sensitive verse insensitive celllines. CCLE: Broad Institute Cancer Cell Line Encyclopedia (seeBarretina, below).

FIGS. 12A-12B: CCNE1 amplified cells with dysfunctional p16 do notrespond to CDK2 inhibition. FIG. 12A: Western blot analysis of p16 inthree gastric cell lines with CCNE1 Amp. FIG. 12B: Percentage of cellsat the S phase 3 days after transfection of CDK2 siRNAs, relative to CtlsiRNA. The cell cycle phase distribution was evaluated by FACS.

FIG. 13: p16 knockdown by siRNA abolishes CDK2 inhibition induced cellcycle suppression in CCNE1 amplified cells. The percentage of S phasecells following p16 knockdown and CDK2 inhibitor treatment, normalizedto cell with Ctl siRNA and DMSO treatment. CCNE1 amplified COV318 cellswere transfected with either Ctl siRNAs or p16 siRNA. 72 hours aftertransfection, cells were treated with 100 nM CDK2 inhibitor Compound A.Cells were harvested and subjected to cell cycle analysis 16 hours aftertreatment.

DETAILED DESCRIPTION Compounds

The present application provides, inter alia, a compound of Formula (I):

or a pharmaceutically acceptable salt thereof, wherein:

n is 0, 1, 2, 3, 4, 5, or 6;

Z¹ is CR⁴R⁵; and Z² is NR^(6z), O, or S; or

Z¹ is CR⁴R⁵, NR⁶, O, or S; and Z² is CR^(4z)R^(5z);

Y is CR⁷ or N;

X¹ is NR^(1x), CR¹, N, or O;

X² is NR^(2x), CR², N, or O;

X³ is N or C;

wherein Ring

is an aromatic ring selected from:

Ring moiety A is selected from (1) C₃₋₁₄ cycloalkyl, wherein 1, 2, 3, or4 ring-forming carbon atoms of said C₃₋₁₄ cycloalkyl can be optionallysubstituted by one or more oxo or sulfido, and (2) 4-14 memberedheterocycloalkyl having 1, 2, 3, or 4 ring-forming heteroatomsindependently selected from N, O, and S, wherein 1, 2, 3, or 4ring-forming carbon or heteroatoms can be optionally substituted by oneor more oxo or sulfido; wherein Ring moiety A is attached to the —NH—group of Formula (I) at a ring member of a saturated or partiallysaturated ring of said C₃₋₁₄ cycloalkyl or said 4-14 memberedheterocycloalkyl;

R¹ is selected from H, D, halo, CN, NO₂, C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆alkynyl, C₁₋₆ haloalkyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10membered heterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10 memberedheterocycloalkyl-C₁₋₄ alkyl, 5-10 membered heteroaryl-C₁₋₄ alkyl,OR^(a1), SR^(a1), NHOR^(a1), C(O)R^(b1), C(O)NR^(c1)R^(d1),C(O)NR^(c1)(OR^(a1)), C(O)OR^(a1), OC(O)R^(b1), OC(O)NR^(c1)R^(d1),NR^(c1)R^(d1), NR^(c1)NR^(c1)R^(d1), NR^(c1)C(O)R^(b1),NR^(c1)C(O)OR^(a1), NR^(c1)C(O)NR^(c1)R^(d1), C(═NR^(e1))R^(b1),C(═NR^(e1))NR^(c1)R^(d1), NR^(c1)C(═NR^(e1))NR^(c1)R^(d1),NR^(c1)C(═NR^(e1))R^(b1), NR^(c1)S(O)NR^(c1)R^(d1), NR^(c1)S(O)R^(b1),NR^(c1)S(O)₂R^(b1), NR^(c1)S(O)(═NR^(e1))R^(b1),NR^(c1)S(O)₂NR^(c1)R^(d1), S(O)R^(b1), S(O)NR^(c1)R^(d1), S(O)₂R^(b1),S(O)₂NR^(c1)R^(d1), OS(O)(═NR^(e1))R^(b1), OS(O)₂R^(b1),S(O)(═NR^(e1))R^(b1), SF₅, P(O)R^(f1)R^(g1), OP(O)(OR^(h1))(OR^(i1)),P(O)(OR^(h1))(OR^(i1)), and BR^(j1)R^(k1); wherein said C₁₋₆ alkyl, C₂₋₆alkenyl, C₂₋₆ alkynyl, C₁₋₆ haloalkyl, C₃₋₁₀ cycloalkyl, 6-10 memberedaryl, 4-10 membered heterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted by 1, 2, 3, or 4 independently selectedR^(1A) substituents;

R^(1x) is selected from H, C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₁₋₆haloalkyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10 memberedheterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl,6-10 membered aryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄alkyl, 5-10 membered heteroaryl-C₁₋₄ alkyl, C(O)R^(b1),C(O)NR^(c1)R^(d1), C(O)NR^(c1)(OR^(a1)), C(O)OR^(a1), C(═NR^(e1))R^(b1),C(═NR^(e1))NR^(c1)R^(d1), S(O)₂R^(b1), and S(O)₂NR^(c1)R^(d1); whereinsaid C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₁₋₆ haloalkyl, C₃₋₁₀cycloalkyl, 6-10 membered aryl, 4-10 membered heterocycloalkyl, 5-10membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 memberedaryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-10membered heteroaryl-C₁₋₄ alkyl are each optionally substituted by 1, 2,3, or 4 independently selected R^(1A) substituents;

R² is selected from H, D, halo, NO₂, CN, C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆alkynyl, C₁₋₆ haloalkyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10membered heterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10 memberedheterocycloalkyl-C₁₋₄ alkyl, 5-10 membered heteroaryl-C₁₋₄ alkyl,OR^(a2), SR^(a2) NHOR^(a2), C(O)R^(b2), C(O)NR^(c2)R^(d2),C(O)NR^(c2)(OR^(a2)), C(O)OR^(a2), OC(O)R^(b2), OC(O)NR^(c2)R^(d2),NR^(c2)R^(d2), NR^(c2)NR^(c2)R^(d2), NR^(c2)C(O)R^(b2),NR^(c2)C(O)OR^(a2), NR^(c2)C(O)NR^(c2)R^(d2), C(═NR^(e2))R^(b2),C(═NR^(e2))NR^(c2)R^(d2), NR^(c2)C(═NR^(e2))NR^(c2)R^(d2),NR^(c2)C(═NR^(e2))R^(b2), NR^(c2)S(O)NR^(c2)R^(d2), NR^(c2)S(O)R^(b2),NR^(c2)S(O)₂R^(b2), NR^(c2)S(O)(═NR^(e2))R^(b2),NR^(c2)S(O)₂NR^(c2)R^(d2), S(O)R^(b2), S(O)NR^(c2)R^(d2), S(O)₂R^(b2),S(O)₂NR^(c2)R^(d2), OS(O)(═NR^(e2))R^(b2), OS(O)₂R^(b2),S(O)(═NR^(e2))R^(b2), SF₅, P(O)R^(f2)R^(g2), OP(O)(OR^(h2))(OR^(i2)),P(O)(OR^(h2))(OR^(i2)), and BR^(j2)R^(k2); wherein said C₁₋₆ alkyl, C₂₋₆alkenyl, C₂₋₆ alkynyl, C₁₋₆ haloalkyl, C₃₋₁₀ cycloalkyl, 6-10 memberedaryl, 4-10 membered heterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted by 1, 2, 3, or 4 independently selectedR^(2A) substituents;

R^(2x) is selected from H, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₂₋₆ alkenyl,C₂₋₆ alkynyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10 memberedheterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl,6-10 membered aryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄alkyl, 5-10 membered heteroaryl-C₁₋₄ alkyl, C(O)R^(b2),C(O)NR^(c2)R^(d2), C(O)NR^(c2)(OR^(a2)), C(O)OR^(a2), C(═NR^(e2))R^(b2),C(═NR^(e2))NR^(c2)R^(d2), S(O)₂R^(b2), and S(O)₂NR^(c2)R^(d2); whereinsaid C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀cycloalkyl, 6-10 membered aryl, 4-10 membered heterocycloalkyl, 5-10membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 memberedaryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-10membered heteroaryl-C₁₋₄ alkyl are each optionally substituted by 1, 2,3, or 4 independently selected R^(2A) substituents;

R³ is selected from H, D, halo, CN, C₁₋₄ alkyl, C₁₋₄ haloalkyl, C₂₋₄alkenyl, C₂₋₄ alkynyl, OH, C₁₋₃ alkoxy, C₁₋₃ haloalkoxy, amino, C₁₋₃alkylamino, di(C₁₋₃ alkyl)amino, and C₃₋₄ cycloalkyl;

R⁴, R^(4z), R⁵ and R^(5z) are independently selected from H, D, halo,CN, C₁₋₄ alkyl, C₁₋₄ haloalkyl, C₂₋₄ alkenyl, C₂₋₄ alkynyl, OH, C₁₋₃alkoxy, C₁₋₃ haloalkoxy, amino, C₁₋₃ alkylamino, di(C₁₋₃ alkyl)amino,cyano-C₁₋₃ alkyl, HO—C₁₋₃ alkyl, C₁₋₃ alkoxy-C₁₋₃ alkyl, and C₃₋₄cycloalkyl;

or R⁴ and R⁵, together with the carbon atom to which they are attached,form a 3-7 membered cycloalkyl ring or a 4-7 membered heterocycloalkylring, which are each optionally substituted by 1, 2, 3, or 4substituents independently selected from D, halo, CN, C₁₋₃ alkyl, C₁₋₃haloalkyl, OH, C₁₋₃ alkoxy, C₁₋₃ haloalkoxy, amino, C₁₋₃ alkylamino,di(C₁₋₃ alkyl)amino, cyano-C₁₋₃ alkyl, HO—C₁₋₃ alkyl, and C₁₋₃alkoxy-C₁₋₃ alkyl;

or R^(4z) and R^(5z), together with the carbon atom to which they areattached, form a 3-7 membered cycloalkyl ring or a 4-7 memberedheterocycloalkyl ring, which are each optionally substituted by 1, 2, 3,or 4 substituents independently selected from D, halo, CN, C₁₋₃ alkyl,C₁₋₃ haloalkyl, OH, C₁₋₃ alkoxy, C₁₋₃ haloalkoxy, amino, C₁₋₃alkylamino, di(C₁₋₃ alkyl)amino, cyano-C₁₋₃ alkyl, HO—C₁₋₃ alkyl, andC₁₋₃ alkoxy-C₁₋₃ alkyl;

R⁶ and R^(6z) are each independently selected from H, C₁₋₄ alkyl, C₁₋₄haloalkyl, C₂₋₄ alkenyl, C₂₋₄ alkynyl, cyano-C₁₋₃ alkyl, HO—C₁₋₃ alkyl,C₁₋₃ alkoxy-C₁₋₃ alkyl, and C₃₋₄ cycloalkyl;

R⁷ is selected from H, D, halo, CN, C₁₋₄ alkyl, C₁₋₄ haloalkyl, C₂₋₄alkenyl, C₂₋₄ alkynyl, OH, C₁₋₃ alkoxy, C₁₋₃ haloalkoxy, amino, C₁₋₃alkylamino, di(C₁₋₃ alkyl)amino, cyano-C₁₋₃ alkyl, HO—C₁₋₃ alkyl, C₁₋₃alkoxy-C₁₋₃ alkyl, and C₃₋₄ cycloalkyl;

each R⁸ is selected from D, halo, CN, NO₂, C₁₋₆ alkyl, C₂₋₆ alkenyl,C₂₋₆ alkynyl, C₁₋₆ haloalkyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10membered heterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10 memberedheterocycloalkyl-C₁₋₄ alkyl, 5-10 membered heteroaryl-C₁₋₄ alkyl,OR^(a8), SR^(a8), NHOR^(a8), C(O)R^(b8), C(O)NR^(c8)R^(d8),C(O)NR^(c8)(OR^(a8)), C(O)OR^(a8), OC(O)R^(b8), OC(O)NR^(c8)R^(d8),NR^(c8)R^(d8), NR^(c8)NR^(c8)R^(d8), NR^(c8)C(O)R^(b8),NR^(c8)C(O)OR^(a8), NR^(c8)C(O)NR^(c8)R^(d8), C(═NR^(e8))R^(b8),C(═NR)NR^(c8)R^(d8), NR^(c8)C(═NR^(e8))NR^(c8)R^(d8),NR^(c8)C(═NR^(e8))R^(b8), NR^(c8)S(O)NR^(c8)R^(d8), NR^(c8)S(O)R^(b8),NR^(c8)S(O)₂R^(b8), NR^(c8)S(O)(═NR^(e8))R^(b8),NR^(c8)S(O)₂NR^(c8)R^(d8), S(O)R^(b8), S(O)NR^(c8)R^(d8), S(O)₂R^(b8),S(O)₂NR^(c8)R^(d8), OS(O)(═NR^(e8))R^(b8), OS(O)₂R^(b8),S(O)(═NR^(e8))R^(b8), SF₅, P(O)R^(f8)R^(g8), OP(O)(OR^(h8))(OR^(i8)),P(O)(OR^(h8))(OR^(i8)), and BR^(j8)R^(k8); wherein said C₁₋₆ alkyl, C₂₋₆alkenyl, C₂₋₆ alkynyl, C₁₋₆ haloalkyl, C₃₋₁₀ cycloalkyl, 6-10 memberedaryl, 4-10 membered heterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted by 1, 2, 3, or 4 independently selectedR^(8A) substituents;

each R^(1A) is independently selected from D, halo, CN, NO₂, C₁₋₆ alkyl,C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₁₋₆ haloalkyl, C₃₋₁₀ cycloalkyl, 6-10membered aryl, 4-10 membered heterocycloalkyl, 5-10 membered heteroaryl,C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10membered heterocycloalkyl-C₁₋₄ alkyl, 5-10 membered heteroaryl-C₁₋₄alkyl, OR^(a11), SR^(a11), NHOR^(a11), C(O)R^(b11), C(O)NR^(c11)R^(d11),C(O)NR^(c11)(OR^(a11)), C(O)OR^(a11), OC(O)R^(b11) OC(O)NR^(c11)R^(d11),NR^(c11)R^(d11), NR^(c11)NR^(c11)R^(d11), NR^(c11)C(O)R^(b11),NR^(c11)C(O)OR^(a11), NR^(c11)C(O)NR^(c11)R^(d11), C(═NR^(e11))R^(b11),C(═NR^(e11))NR^(c11)R^(d11), NR^(c11)C(═NR^(e11))NR^(c11)R^(d11),NR^(c11)C(═NR^(e11))R^(b11), NR^(c11)S(O)NR^(c11)R^(d11),NR^(c11)S(O)R^(b11), NR^(c11)S(O)₂R^(b11),NR^(c11)S(O)(═NR^(e11))R^(b11), NR^(c11)S(O)₂NR^(c11)R^(d11),S(O)R^(b11), S(O)NR^(c11)R^(d11), S(O)₂R^(b11), S(O)₂NR^(c11)R^(d11),OS(O)(═NR^(e11))R^(b11), OS(O)₂R^(b11), S(O)(═NR^(e11))R^(b11), SF₅,P(O)R^(f11)R^(g11), OP(O)(OR^(h11))(OR^(i11)), P(O)(OR^(h11))(OR^(i11))and BR^(j11)R^(k11), wherein said C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆alkynyl, C₁₋₆ haloalkyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10membered heterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(1B) substituents;

each R^(1B) is independently selected from D, halo, CN, NO₂, C₁₋₆ alkyl,C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄alkyl, phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, 5-6membered heteroaryl-C₁₋₄ alkyl, OR^(a12), SR^(a12), NHOR^(a12),C(O)R^(b12), C(O)NR^(c12)R^(d12), C(O)NR^(c12)(OR^(a12)), C(O)OR^(a12),OC(O)R^(b12), OC(O)NR^(c12)R^(d12), NR^(c12)R^(d12),NR^(c12)NR^(c12)R^(d12), NR^(c12)C(O)R^(b12), NR^(c12)C(O)OR^(a12),NR^(c12)C(O)NR^(c12)R^(d12), C(═NR^(e12))R^(b12),C(═NR^(e12))NR^(c12)R^(d12), NR^(c12)C(═NR^(e12))NR^(c12)R^(d12),NR^(c12)C(═NR^(e12))R^(b12), NR^(c12)S(O)NR^(c12)R^(d12),NR^(c12)S(O)R^(b12), NR^(c12)S(O)₂R^(b12),R^(c12)S(O)(═NR^(e12))R^(b12), NR^(c12)S(O)₂NR^(c12)R^(d12),S(O)R^(b12), S(O)NR^(c12)R^(d12), S(O)₂R^(b12), S(O)₂NR^(c12)R^(d12),OS(O)(═NR^(e12))R^(b12), OS(O)₂R^(b12), S(O)(═NR^(e12))R^(b12), SF₅,P(O)R^(f12)R^(gl2), OP(O)(OR^(h12))(OR^(i12)), P(O)(OR^(h12))(OR^(i12)),and BR^(j12)R^(k12), wherein said C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆alkynyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl are each optionally substituted with 1,2, 3, or 4 independently selected R^(G) substituents;

each R^(2A) is independently selected from D, halo, CN, NO₂, C₁₋₆ alkyl,C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₁₋₆ haloalkyl, C₃₋₁₀ cycloalkyl, 6-10membered aryl, 4-10 membered heterocycloalkyl, 5-10 membered heteroaryl,C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10membered heterocycloalkyl-C₁₋₄ alkyl, 5-10 membered heteroaryl-C₁₋₄alkyl, OR^(a21), SR^(a21), NHOR^(a21), C(O)R^(b21), C(O)NR^(c21)R^(d21),C(O)NR^(c21)(OR^(a21)), C(O)OR^(a21), OC(O)R^(b21),OC(O)NR^(c21)R^(d21), NR^(c21)R^(d21), NR^(c21)NR^(c21)R^(d21),NR^(c21)C(O)R^(b21), NR^(c21)C(O)OR^(a21), NR^(c21)C(O)NR^(c21)R^(d21),C(═NR^(e21))R^(b21), C(═NR^(e21))NR^(c21)R^(d21),NR^(c21)C(═NR^(e21))NR^(c21)R^(d21), NR^(c21)C(═NR^(e21))R^(b21),NR^(c21)S(O)NR^(c21)R^(d21), NR^(c21)S(O)R^(b21), NR^(c21)S(O)₂R^(b21),NR^(c21)S(O)(═NR^(e21))R^(b21), NR^(c21)S(O)₂NR^(c21)R^(d21),S(O)R^(b21), S(O)NR^(c21)R^(d21), S(O)₂R^(b21), S(O)₂NR^(c21)R^(d21),OS(O)(═NR^(e21))R^(b21), OS(O)₂R^(b21), S(O)(═NR^(e21))R^(b21), SF₅,P(O)R^(f21)R^(g21), OP(O)(OR^(h21))(OR^(i21)), P(O)(OR^(h21))(OR^(i21)),and BR^(j2)R^(k21), wherein said C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl,C₁₋₆ haloalkyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10 memberedheterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl,6-10 membered aryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl are each optionallysubstituted with 1, 2, 3, or 4 independently selected R^(2B)substituents;

each R^(2B) is independently selected from D, halo, CN, NO₂, C₁₋₆ alkyl,C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄alkyl, phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, 5-6membered heteroaryl-C₁₋₄ alkyl, OR^(a22), SR^(a22), NHOR^(a22),C(O)R^(b22), C(O)NR^(c22)R^(d22), C(O)NR^(c22)(OR^(a22)), C(O)OR^(a22),OC(O)R^(b22), OC(O)NR^(c22)R^(d22), NR^(c22)R^(d22),NR^(c22)NR^(c22)R^(d22), NR^(c22)C(O)R^(b22), NR^(c22)C(O)OR^(a22),NR^(c22)C(O)NR^(c22)R^(d22), C(═NR^(e22))R^(b22),C(═NR^(e22))NR^(c22)R^(d22), NR^(c22)C(═NR^(e22))NR^(c22)R^(d22),NR^(c22)C(═NR^(e22))R^(b22), NR^(c22)S(O)NR^(c22)R^(d22),NR^(c22)S(O)R^(b22), NR^(c22)S(O)₂R^(b22),NR^(c22)S(O)(═NR^(e22))R^(b22), NR^(c22)S(O)₂NR^(c22)R^(d22),S(O)R^(b22), S(O)NR^(c22)R^(d22), S(O)₂R^(b22), S(O)₂NR^(c22)R^(d22),OS(O)(═NR^(e22))R^(b22), OS(O)₂R^(b22), S(O)(═NR^(e22))R^(b22), SF₅,P(O)R^(f22)R^(g22), OP(O)(OR^(h2))(OR^(i22)), P(O)(OR^(h22))(OR^(i22)),and BR^(j22)R^(k22), wherein said C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆alkynyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl are each optionally substituted with 1,2, 3, or 4 independently selected R^(G) substituents;

each R^(8A) is selected from D, halo, CN, NO₂, C₁₋₆ alkyl, C₂₋₆ alkenyl,C₂₋₆ alkynyl, C₁₋₆ haloalkyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10membered heterocycloalkyl, 5-membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10 memberedheterocycloalkyl-C₁₋₄ alkyl, 5-10 membered heteroaryl-C₁₋₄ alkyl,OR^(a81), SR^(a81), NHOR^(a81), C(O)R^(b81), C(O)NR^(c81)R^(d81),C(O)NR^(c81)(OR^(a81)), C(O)OR^(a81), OC(O)R^(b81),OC(O)NR^(c81)R^(d81), NR^(c81)R^(d81), NR^(c81)NR^(c81)R^(d81),NR^(c81)C(O)R^(b81), NR^(c81)C(O)OR^(a81), NR^(c81)C(O)NR^(c81)R^(d81),C(═NR^(e81))R^(b81), C(═NR^(e81))NR^(c81)R^(d81),NR^(c81)C(═NR^(e81))NR^(c81)R^(d81), NR^(c81)C(═NR^(e81))R^(b81),NR^(c81)S(O)NR^(c81)R^(d81), NR^(c81)S(O)R^(b81), NR^(c81)S(O)₂R^(b81),NR^(c81)S(O)(═NR^(e81))R^(b81), NR^(c81)S(O)₂NR^(c81)R^(d81),S(O)R^(b81), S(O)NR^(c81)R^(d81), S(O)₂R^(b81), S(O)₂NR^(c81)R^(d81),OS(O)(═NR^(e81))R^(b81), OS(O)₂R^(b81), S(O)(═NR^(e81))R^(b81), SF₅,P(O)R^(f81)R^(g81), OP(O)(OR^(h81))(OR^(i81)), P(O)(OR^(h81))(OR^(i81)),and BR^(j81)R^(k81); wherein said C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆alkynyl, C₁₋₆ haloalkyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10membered heterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted by 1, 2, 3, or 4 independently selectedR^(8B) substituents;

each R^(8B) is selected from D, halo, CN, NO₂, C₁₋₆ alkyl, C₂₋₆ alkenyl,C₂₋₆ alkynyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, 5-6membered heteroaryl-C₁₋₄ alkyl, OR^(a82), SR^(a82), NHOR^(a82),C(O)R^(b82), C(O)NR^(c82)R^(d82), C(O)NR^(c82)(OR^(a82)), C(O)OR^(a82),OC(O)R^(b82), OC(O)NR^(c82)R^(d82), NR^(c82)R^(d82),NR^(c82)NR^(c82)NR^(d82), NR^(c82)C(O)R^(b82),NR^(c82)C(O)OR^(c82)R^(d82), C(O)NR^(c82)R^(d82), C(═NR^(e82))R^(b82),C(═NR^(e82))R^(c82)R^(d82), NR^(c82)C(═NR^(e82))NR^(c82)R^(d82),NR^(c82)C(═NR^(e82))R^(b82), NR^(c82)S(O)NR^(c82)R^(d82),NR^(c82)S(O)R^(b82), NR^(c82)S(O)₂R^(b82),NR^(c82)S(O)(═NR^(e82))R^(b82), NR^(c82)S(O)₂NR^(c82)R^(d82),S(O)R^(b82), S(O)NR^(c82)R^(d82), S(O)₂R^(b82), S(O)₂NR^(c82)R^(d82),OS(O)(═NR^(e82))R^(b82), OS(O)₂R^(b82), S(O)(═NR^(e82))R^(b82), SF₅,P(O)R^(f82)R^(g82), OP(O)(OR^(h82))(OR^(i82)), P(O)(OR^(h82))(OR^(i82)),and BR^(j82)R^(k82); wherein said C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆alkynyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl are each optionally substituted by 1, 2,3, or 4 independently selected R^(G) substituents;

each R^(a1), R^(b1), and R^(d1) is independently selected from H, C₁₋₆alkyl, C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀ cycloalkyl,6-10 membered aryl, 4-10 membered heterocycloalkyl, 5-10 memberedheteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl,4-10 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-10 memberedheteroaryl-C₁₋₄ alkyl, wherein said C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆alkynyl, C₁₋₆ haloalkyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10membered heterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(1A) substituents;

or, any R^(c1) and R^(d1) attached to the same N atom, together with theN atom to which they are attached, form a 4-10 membered heterocycloalkylgroup, which is optionally substituted with 1, 2, 3, or 4 independentlyselected R^(1A) substituents;

each R^(b1) is independently selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10membered heterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl,which are each optionally substituted with 1, 2, 3, or 4 independentlyselected R^(1A) substituents;

each R^(e1) is independently selected from H, OH, CN, C₁₋₆ alkyl, C₁₋₆alkoxy, C₁₋₆ haloalkyl, C₁₋₆ haloalkoxy, C₂₋₆ alkenyl, C₂₋₆ alkynyl,C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10 membered heterocycloalkyl,5-10 membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 memberedaryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄ alkyl, and5-membered heteroaryl-C₁₋₄ alkyl;

each R^(f1) and R^(g1) are independently selected from H, C₁₋₆ alkyl,C₁₋₆ alkoxy, C₁₋₆ haloalkyl, C₁₋₆ haloalkoxy, C₂₋₆ alkenyl, C₂₋₆alkynyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10 memberedheterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl,6-10 membered aryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄alkyl, and 5-membered heteroaryl-C₁₋₄ alkyl;

each R^(h1) and R^(i1) is independently selected from H, C₁₋₆ alkyl,C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀ cycloalkyl, 6-10membered aryl, 4-10 membered heterocycloalkyl, 5-10 membered heteroaryl,C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10membered heterocycloalkyl-C₁₋₄ alkyl, and 5-10 membered heteroaryl-C₁₋₄alkyl;

each R^(j1) and R^(k1) is independently selected from OH, C₁₋₆ alkoxy,and C₁₋₆ haloalkoxy;

or any R^(j1) and R^(k1) attached to the same B atom, together with theB atom to which they are attached, form a 5- or 6-memberedheterocycloalkyl group optionally substituted with 1, 2, 3, or 4substituents independently selected from C₁₋₆ alkyl and C₁₋₆ haloalkyl;

each R^(a11), R^(c11), and R^(d11) is independently selected from H,C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀cycloalkyl, 6-10 membered aryl, 4-10 membered heterocycloalkyl, 5-10membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 memberedaryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-10membered heteroaryl-C₁₋₄ alkyl, wherein said C₁₋₆ alkyl, C₂₋₆ alkenyl,C₂₋₆ alkynyl, C₁₋₆ haloalkyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10membered heterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(8B) substituents;

or, any R^(c11) and R^(d11) attached to the same N atom, together withthe N atom to which they are attached, form a 4-7 memberedheterocycloalkyl group, which is optionally substituted with 1, 2, 3, or4 independently selected R^(8B) substituents;

each R^(b11) is independently selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10membered heterocycloalkyl, 5-membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl,which are each optionally substituted with 1, 2, 3, or 4 independentlyselected R^(8B) substituents;

each R^(e11) is independently selected from H, OH, CN, C₁₋₆ alkyl, C₁₋₆alkoxy, C₁₋₆ haloalkyl, C₁₋₆ haloalkoxy, C₂₋₆ alkenyl, C₂₋₆ alkynyl,C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10 membered heterocycloalkyl,5-10 membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 memberedaryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄ alkyl, and5-membered heteroaryl-C₁₋₄ alkyl;

each R^(f11) and R^(g11) are independently selected from H, C₁₋₆ alkyl,C₁₋₆ alkoxy, C₁₋₆ haloalkyl, C₁₋₆ haloalkoxy, C₂₋₆ alkenyl, C₂₋₆alkynyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10 memberedheterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl,6-10 membered aryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄alkyl, and 5-membered heteroaryl-C₁₋₄ alkyl;

each R^(h1) and R^(i11) is independently selected from H, C₁₋₆ alkyl,C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀ cycloalkyl, 6-10membered aryl, 4-10 membered heterocycloalkyl, 5-10 membered heteroaryl,C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10membered heterocycloalkyl-C₁₋₄ alkyl, and 5-10 membered heteroaryl-C₁₋₄alkyl;

each R^(j11) and R^(k11) is independently selected from OH, C₁₋₆ alkoxy,and C₁₋₆ haloalkoxy;

or any R^(j11) and R^(k11) attached to the same B atom, together withthe B atom to which they are attached, form a 5- or 6-memberedheterocycloalkyl group optionally substituted with 1, 2, 3, or 4substituents independently selected from C₁₋₆ alkyl and C₁₋₆ haloalkyl;

each R^(a12), R^(c12), and R^(d12) is independently selected from H,C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₇ cycloalkyl,phenyl, 4-7 membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl,wherein said C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(G) substituents;

or, any R^(c12) and R^(d12) attached to the same N atom, together withthe N atom to which they are attached, form a 4-7 memberedheterocycloalkyl group, which is optionally substituted with 1, 2, 3, or4 independently selected R^(G) substituents;

each R^(b12) is independently selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl, which are each optionally substitutedwith 1, 2, 3, or 4 independently selected R^(G) substituents;

each R^(e12) is independently selected from H, OH, CN, C₁₋₆ alkyl, C₁₋₆alkoxy, C₁₋₆ haloalkyl, C₁₋₆ haloalkoxy, C₂₋₆ alkenyl, C₂₋₆ alkynyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl;

each R^(f12) and R^(g12) are independently selected from H, C₁₋₆ alkyl,C₁₋₆ alkoxy, C₁₋₆ haloalkyl, C₁₋₆ haloalkoxy, C₂₋₆ alkenyl, C₂₋₆alkynyl, C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄alkyl;

each R^(h12) and R^(i12) is independently selected from H, C₁₋₆ alkyl,C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄alkyl, phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and5-6 membered heteroaryl-C₁₋₄ alkyl;

each R^(j12) and R^(k12) is independently selected from OH, C₁₋₆ alkoxy,and C₁₋₆ haloalkoxy;

or any R^(j12) and R^(k12) attached to the same B atom, together withthe B atom to which they are attached, form a 5- or 6-memberedheterocycloalkyl group optionally substituted with 1, 2, 3, or 4substituents independently selected from C₁₋₆ alkyl and C₁₋₆ haloalkyl;

each R^(a2), R^(c2), and R^(d2) is independently selected from H, C₁₋₆alkyl, C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀ cycloalkyl,6-10 membered aryl, 4-10 membered heterocycloalkyl, 5-10 memberedheteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl,4-10 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-10 memberedheteroaryl-C₁₋₄ alkyl, wherein said C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆alkynyl, C₁₋₆ haloalkyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10membered heterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(2A) substituents;

or, any R^(c2) and R^(d2) attached to the same N atom, together with theN atom to which they are attached, form a 4-10 membered heterocycloalkylgroup, which is optionally substituted with 1, 2, 3, or 4 independentlyselected R^(2A) substituents;

each R^(b2) is independently selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10membered heterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl,which are each optionally substituted with 1, 2, 3, or 4 independentlyselected R^(2A) substituents;

each R^(e2) is independently selected from H, OH, CN, C₁₋₆ alkyl, C₁₋₆alkoxy, C₁₋₆ haloalkyl, C₁₋₆ haloalkoxy, C₂₋₆ alkenyl, C₂₋₆ alkynyl,C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10 membered heterocycloalkyl,5-10 membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 memberedaryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄ alkyl, and5-membered heteroaryl-C₁₋₄ alkyl;

each R^(f2) and R^(g2) are independently selected from H, C₁₋₆ alkyl,C₁₋₆ alkoxy, C₁₋₆ haloalkyl, C₁₋₆ haloalkoxy, C₂₋₆ alkenyl, C₂₋₆alkynyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10 memberedheterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl,6-10 membered aryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄alkyl, and 5-membered heteroaryl-C₁₋₄ alkyl;

each R^(j1) and R^(k2) is independently selected from H, C₁₋₆ alkyl,C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀ cycloalkyl, 6-10membered aryl, 4-10 membered heterocycloalkyl, 5-10 membered heteroaryl,C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10membered heterocycloalkyl-C₁₋₄ alkyl, and 5-10 membered heteroaryl-C₁₋₄alkyl;

each R^(j2) and R^(k2) is independently selected from OH, C₁₋₆ alkoxy,and C₁₋₆ haloalkoxy;

or any R^(j2) and R^(k2) attached to the same B atom, together with theB atom to which they are attached, form a 5- or 6-memberedheterocycloalkyl group optionally substituted with 1, 2, 3, or 4substituents independently selected from C₁₋₆ alkyl and C₁₋₆ haloalkyl;

each R^(a21), R^(c21), and R^(d21) is independently selected from H,C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀cycloalkyl, 6-10 membered aryl, 4-10 membered heterocycloalkyl, 5-10membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 memberedaryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-10membered heteroaryl-C₁₋₄ alkyl, wherein said C₁₋₆ alkyl, C₂₋₆ alkenyl,C₂₋₆ alkynyl, C₁₋₆ haloalkyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10membered heterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(2B) substituents;

or, any R^(c21) and R^(d21) attached to the same N atom, together withthe N atom to which they are attached, form a 4-7 memberedheterocycloalkyl group, which is optionally substituted with 1, 2, 3, or4 independently selected R^(2B) substituents;

each R^(b21) is independently selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10membered heterocycloalkyl, 5-membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl,which are each optionally substituted with 1, 2, 3, or 4 independentlyselected R^(2B) substituents;

each R^(e21) is independently selected from H, OH, CN, C₁₋₆ alkyl, C₁₋₆alkoxy, C₁₋₆ haloalkyl, C₁₋₆ haloalkoxy, C₂₋₆ alkenyl, C₂₋₆ alkynyl,C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10 membered heterocycloalkyl,5-10 membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 memberedaryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄ alkyl, and5-membered heteroaryl-C₁₋₄ alkyl;

each R^(f21) and R^(g21) are independently selected from H, C₁₋₆ alkyl,C₁₋₆ alkoxy, C₁₋₆ haloalkyl, C₁₋₆ haloalkoxy, C₂₋₆ alkenyl, C₂₋₆alkynyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10 memberedheterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl,6-10 membered aryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄alkyl, and 5-membered heteroaryl-C₁₋₄ alkyl;

each R^(h21) and R^(i21) is independently selected from H, C₁₋₆ alkyl,C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀ cycloalkyl, 6-10membered aryl, 4-10 membered heterocycloalkyl, 5-10 membered heteroaryl,C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10membered heterocycloalkyl-C₁₋₄ alkyl, and 5-10 membered heteroaryl-C₁₋₄alkyl;

each R^(j21) and R^(k21) is independently selected from OH, C₁₋₆ alkoxy,and C₁₋₆ haloalkoxy;

or any R^(j21) and R^(k21) attached to the same B atom, together withthe B atom to which they are attached, form a 5- or 6-memberedheterocycloalkyl group optionally substituted with 1, 2, 3, or 4substituents independently selected from C₁₋₆ alkyl and C₁₋₆ haloalkyl;

each R^(a22), R^(c22), and R^(d22) is independently selected from H,C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₇ cycloalkyl,phenyl, 4-7 membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl,wherein said C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(G) substituents;

or, any R^(c22) and R^(d22) attached to the same N atom, together withthe N atom to which they are attached, form a 4-7 memberedheterocycloalkyl group, which is optionally substituted with 1, 2, 3, or4 independently selected R^(G) substituents;

each R^(b22) is independently selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl, which are each optionally substitutedwith 1, 2, 3, or 4 independently selected R^(G) substituents;

each R^(e22) is independently selected from H, OH, CN, C₁₋₆ alkyl, C₁₋₆alkoxy, C₁₋₆ haloalkyl, C₁₋₆ haloalkoxy, C₂₋₆ alkenyl, C₂₋₆ alkynyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl;

each R^(f22) and R^(g22) are independently selected from H, C₁₋₆ alkyl,C₁₋₆ alkoxy, C₁₋₆ haloalkyl, C₁₋₆ haloalkoxy, C₂₋₆ alkenyl, C₂₋₆alkynyl, C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄alkyl;

each R^(h22) and R^(i22) is independently selected from H, C₁₋₆ alkyl,C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄alkyl, phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and5-6 membered heteroaryl-C₁₋₄ alkyl;

each R^(j22) and R^(k22) is independently selected from OH, C₁₋₆ alkoxy,and C₁₋₆ haloalkoxy;

or any R^(j22) and R^(k22) attached to the same B atom, together withthe B atom to which they are attached, form a 5- or 6-memberedheterocycloalkyl group optionally substituted with 1, 2, 3, or 4substituents independently selected from C₁₋₆ alkyl and C₁₋₆ haloalkyl;

each R^(a8), R^(c8), and R^(d8) is independently selected from H, C₁₋₆alkyl, C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀ cycloalkyl,6-10 membered aryl, 4-10 membered heterocycloalkyl, 5-10 memberedheteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl,4-10 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-10 memberedheteroaryl-C₁₋₄ alkyl, wherein said C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆alkynyl, C₁₋₆ haloalkyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10membered heterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(8A) substituents;

or, any R^(c8) and R^(d8) attached to the same N atom, together with theN atom to which they are attached, form a 4-10 membered heterocycloalkylgroup, which is optionally substituted with 1, 2, 3, or 4 independentlyselected R^(8A) substituents;

each R^(b8) is independently selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10membered heterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl,which are each optionally substituted with 1, 2, 3, or 4 independentlyselected R^(8A) substituents;

each R^(e8) is independently selected from H, OH, CN, C₁₋₆ alkyl, C₁₋₆alkoxy, C₁₋₆ haloalkyl, C₁₋₆ haloalkoxy, C₂₋₆ alkenyl, C₂₋₆ alkynyl,C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10 membered heterocycloalkyl,5-10 membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 memberedaryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄ alkyl, and5-membered heteroaryl-C₁₋₄ alkyl;

each R^(f8) and R^(g8) are independently selected from H, C₁₋₆ alkyl,C₁₋₆ alkoxy, C₁₋₆ haloalkyl, C₁₋₆ haloalkoxy, C₂₋₆ alkenyl, C₂₋₆alkynyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10 memberedheterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl,6-10 membered aryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄alkyl, and 5-membered heteroaryl-C₁₋₄ alkyl;

each R^(h8) and R^(i8) is independently selected from H, C₁₋₆ alkyl,C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀ cycloalkyl, 6-10membered aryl, 4-10 membered heterocycloalkyl, 5-10 membered heteroaryl,C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10membered heterocycloalkyl-C₁₋₄ alkyl, and 5-10 membered heteroaryl-C₁₋₄alkyl;

each R^(j8) and R^(k8) is independently selected from OH, C₁₋₆ alkoxy,and C₁₋₆ haloalkoxy;

or any R^(j8) and R^(k8) attached to the same B atom, together with theB atom to which they are attached, form a 5- or 6-memberedheterocycloalkyl group optionally substituted with 1, 2, 3, or 4substituents independently selected from C₁₋₆ alkyl and C₁₋₆ haloalkyl;

each R^(a81), R^(c81), and R^(d81) is independently selected from H,C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀cycloalkyl, 6-10 membered aryl, 4-10 membered heterocycloalkyl, 5-10membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 memberedaryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-10membered heteroaryl-C₁₋₄ alkyl, wherein said C₁₋₆ alkyl, C₂₋₆ alkenyl,C₂₋₆ alkynyl, C₁₋₆ haloalkyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10membered heterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(8B) substituents;

or, any R^(c81) and R^(d81) attached to the same N atom, together withthe N atom to which they are attached, form a 4-7 memberedheterocycloalkyl group, which is optionally substituted with 1, 2, 3, or4 independently selected R^(8B) substituents;

each R^(b81) is independently selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10membered heterocycloalkyl, 5-membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl,which are each optionally substituted with 1, 2, 3, or 4 independentlyselected R^(8B) substituents;

each R^(c81) is independently selected from H, OH, CN, C₁₋₆ alkyl, C₁₋₆alkoxy, C₁₋₆ haloalkyl, C₁₋₆ haloalkoxy, C₂₋₆ alkenyl, C₂₋₆ alkynyl,C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10 membered heterocycloalkyl,5-10 membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 memberedaryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄ alkyl, and5-membered heteroaryl-C₁₋₄ alkyl;

each R^(f81) and R^(g81) are independently selected from H, C₁₋₆ alkyl,C₁₋₆ alkoxy, C₁₋₆ haloalkyl, C₁₋₆ haloalkoxy, C₂₋₆ alkenyl, C₂₋₆alkynyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10 memberedheterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl,6-10 membered aryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄alkyl, and 5-membered heteroaryl-C₁₋₄ alkyl;

each R^(h81) and R^(i81) is independently selected from H, C₁₋₆ alkyl,C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀ cycloalkyl, 6-10membered aryl, 4-10 membered heterocycloalkyl, 5-10 membered heteroaryl,C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10membered heterocycloalkyl-C₁₋₄ alkyl, and 5-10 membered heteroaryl-C₁₋₄alkyl;

each R^(j81) and R^(k81) is independently selected from OH, C₁₋₆ alkoxy,and C₁₋₆ haloalkoxy;

or any R^(j81) and R^(k81) attached to the same B atom, together withthe B atom to which they are attached, form a 5- or 6-memberedheterocycloalkyl group optionally substituted with 1, 2, 3, or 4substituents independently selected from C₁₋₆ alkyl and C₁₋₆ haloalkyl;

each R^(a82), R^(c82), and R^(d82) is independently selected from H,C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₇ cycloalkyl,phenyl, 4-7 membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl,wherein said C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(G) substituents;

or, any R^(c82) and R^(d82) attached to the same N atom, together withthe N atom to which they are attached, form a 4-7 memberedheterocycloalkyl group, which is optionally substituted with 1, 2, 3, or4 independently selected R^(G) substituents;

each R^(b82) is independently selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl, which are each optionally substitutedwith 1, 2, 3, or 4 independently selected R^(G) substituents;

each R^(e82) is independently selected from H, OH, CN, C₁₋₆ alkyl, C₁₋₆alkoxy, C₁₋₆ haloalkyl, C₁₋₆ haloalkoxy, C₂₋₆ alkenyl, C₂₋₆ alkynyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl;

each R^(f82) and R^(g82) are independently selected from H, C₁₋₆ alkyl,C₁₋₆ alkoxy, C₁₋₆ haloalkyl, C₁₋₆ haloalkoxy, C₂₋₆ alkenyl, C₂₋₆alkynyl, C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄alkyl;

each R^(h82) and R^(i82) is independently selected from H, C₁₋₆ alkyl,C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄alkyl, phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and5-6 membered heteroaryl-C₁₋₄ alkyl;

each R^(j82) and R^(k82) is independently selected from OH, C₁₋₆ alkoxy,and C₁₋₆ haloalkoxy;

or any R^(j82) and R^(k82) attached to the same B atom, together withthe B atom to which they are attached, form a 5- or 6-memberedheterocycloalkyl group optionally substituted with 1, 2, 3, or 4substituents independently selected from C₁₋₆ alkyl and C₁₋₆ haloalkyl;and

each R^(G) is independently selected from OH, NO₂, CN, halo, C₁₋₃ alkyl,C₂₋₃ alkenyl, C₂₋₃ alkynyl, C₁₋₃ haloalkyl, cyano-C₁₋₃ alkyl, HO—C₁₋₃alkyl, C₁₋₃ alkoxy-C₁₋₃ alkyl, C₃₋₇ cycloalkyl, C₁₋₃ alkoxy, C₁₋₃haloalkoxy, amino, C₁₋₃ alkylamino, di(C₁₋₃ alkyl)amino, thio, C₁₋₃alkylthio, C₁₋₃ alkylsulfinyl, C₁₋₃ alkylsulfonyl, carbamyl, C₁₋₃alkylcarbamoyl, di(C₁₋₃ alkyl)carbamyl, carboxy, C₁₋₃ alkylcarbonyl,C₁₋₃ alkoxycarbonyl, C₁₋₃ alkylcarbonyloxy, C₁₋₃ alkylcarbonylamino,C₁₋₃ alkoxycarbonylamino, C₁₋₃ alkylaminocarbonyloxy, di(C₁₋₃alkyl)aminocarbonyloxy, C₁₋₃ alkylsulfonylamino, aminosulfonyl, C₁₋₃alkylaminosulfonyl, di(C₁₋₃ alkyl)aminosulfonyl, aminosulfonylamino,C₁₋₃ alkylaminosulfonylamino, di(C₁₋₃ alkyl)aminosulfonylamino,aminocarbonylamino, C₁₋₃ alkylaminocarbonylamino, and di(C₁₋₃alkyl)aminocarbonylamino.

In some embodiments, X¹ is NR^(1x).

In some embodiments, X¹ is CR¹.

In some embodiments, X² is NR^(2x).

In some embodiments, X² is CR².

In some embodiments, X³ is C.

In some embodiments, Ring

In some embodiments, Ring

In some embodiments, R¹ is selected from H, halo, CN, NO₂, C₁₋₆ alkyl,C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₁₋₆ haloalkyl, C₃₋₁₀ cycloalkyl, 6-10membered aryl, 4-10 membered heterocycloalkyl, 5-10 membered heteroaryl,C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10membered heterocycloalkyl-C₁₋₄ alkyl, 5-10 membered heteroaryl-C₁₋₄alkyl, OR^(a1), SR^(a1), NHOR^(a1), C(O)R^(b1), C(O)NR^(c1)R^(d1),C(O)OR^(a1), OC(O)R^(b1), OC(O)NR^(c1)R^(d1), NR^(c1)R^(d1),NR^(c1)C(O)R^(b1), NR^(c1)C(O)OR^(a1), NR^(c1)C(O)NR^(c1)R^(d1),NR^(c1)S(O)₂R^(b1), NR^(c1)S(O)₂NR^(c1)R^(d1), S(O)₂R^(b1), andS(O)₂NR^(c1)R^(d1); wherein said C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl,C₁₋₆ haloalkyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10 memberedheterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl,6-10 membered aryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl are each optionallysubstituted by 1, 2, 3, or 4 independently selected R^(1A) substituents.

In some embodiments, R¹ is selected from H, halo, CN, C₁₋₆ alkyl, C₁₋₆haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7membered heterocycloalkyl-C₁₋₄ alkyl, 5-6 membered heteroaryl-C₁₋₄alkyl, OR^(a1), SR^(a1), C(O)R^(b1), C(O)NR^(c1)R^(d1), C(O)OR^(a1),OC(O)R^(b1), OC(O)NR^(c1)R^(d1), NR^(c1)R^(d1), NR^(c1)C(O)R^(b1),NR^(c)C(O)OR^(a1), NR^(c1)C(O)NR^(c1)R^(d1), NR^(c1)S(O)₂R^(b1),NR^(c1)S(O)₂NR^(c1)R^(d1), S(O)₂R^(b1), and S(O)₂NR^(c1)R^(d1); whereinsaid C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl are each optionally substituted by 1, 2,3, or 4 independently selected R^(1A) substituents.

In some embodiments, R¹ is selected from H, halo, CN, C₁₋₆ alkyl, C₁₋₆haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6membered heteroaryl, OR^(a1), C(O)R^(b1), C(O)NR^(c1)R^(d1),C(O)OR^(a1), OC(O)R^(b1), OC(O)NR^(c1)R^(d1), NR^(c1)R^(d1),NR^(c1)C(O)R^(b1), NR^(c1)S(O)₂R^(b1), S(O)₂R^(b1), andS(O)₂NR^(c1)R^(d1); wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, and 5-6 memberedheteroaryl are each optionally substituted by 1, 2, or 3 independentlyselected R^(1A) substituents.

In some embodiments, R¹ is selected from H, halo, CN, C₁₋₆ alkyl, C₁₋₆haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄alkyl; wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl,4-7 membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted by 1, 2, 3, or 4 independently selectedR^(1A) substituents.

In some embodiments, R¹ is selected from H, halo, C₁₋₆ alkyl, C₁₋₆haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, and5-6 membered heteroaryl;

wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, and 5-6 membered heteroaryl are eachoptionally substituted by 1, 2, or 3 independently selected R^(1A)substituents.

In some embodiments, R¹ is selected from H, halo, C₁₋₆ alkyl, phenyl,and 5-6 membered heteroaryl, wherein said C₁₋₆ alkyl, phenyl and 5-6membered heteroaryl are each optionally substituted by 1 or 2independently selected R^(1A) substituents.

In some embodiments, R¹ is selected from H, Br, methyl, ethyl,isopropyl, phenyl, 2-fluorophenyl, 2,5-difluorophenyl, and1,3-dimethylpyrazol-4-yl.

In some embodiments, R^(1x) is selected from H, C₁₋₆ alkyl, C₁₋₆haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄alkyl; wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl,4-7 membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted by 1, 2, or 3 independently selected R^(1A)substituents.

In some embodiments, R^(1x) is selected from H, C₁₋₆ alkyl, C₁₋₆haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, and5-6 membered heteroaryl;

wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, and 5-6 membered heteroaryl are eachoptionally substituted by 1, 2, or 3 independently selected R^(1A)substituents.

In some embodiments, R^(1x) is selected from H, C₁₋₆ alkyl, C₃₋₇cycloalkyl, phenyl, and 4-7 membered heterocycloalkyl, wherein said C₁₋₆alkyl, C₃₋₇ cycloalkyl, phenyl, and 4-7 membered heterocycloalkyl areeach optionally substituted by 1 or 2 independently selected R^(1A)substituents.

In some embodiments, RX is selected from methyl, —CH₂CH₂OH, cyclopropyl,2,5-difluorophenyl, and tetrahydrofuran-3-yl.

In some embodiments:

each R^(a1), R^(c1), and R^(d1) is independently selected from H, C₁₋₆alkyl, C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀ cycloalkyl,6-10 membered aryl, 4-10 membered heterocycloalkyl, 5-10 memberedheteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl,4-10 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-10 memberedheteroaryl-C₁₋₄ alkyl, wherein said C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆alkynyl, C₁₋₆ haloalkyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10membered heterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(1A) substituents;

each R^(b1) is independently selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10membered heterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl,which are each optionally substituted with 1, 2, 3, or 4 independentlyselected R^(1A) substituents;

each R^(1A) is independently selected from halo, CN, NO₂, C₁₋₆ alkyl,C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄alkyl, phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, 5-6membered heteroaryl-C₁₋₄ alkyl, OR^(a11), SR^(a11), C(O)R^(b11),C(O)NR^(c11)R^(d11), C(O)OR^(d11), OC(O)R^(b11), OC(O)NR^(c11)R^(d11),NR^(c11)R^(d11), NR^(c11)C(O)R^(b11), NR^(c11)C(O)OR^(d11),NR^(c11)C(O)NR^(c11)R^(d11), NR^(c11)S(O)₂R^(b11),NR^(c11)S(O)₂NR^(c11)R^(d11), S(O)₂R^(b11), and S(O)₂NR^(c11)R^(d11),wherein said C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl, and5-10 membered heteroaryl-C₁₋₄ alkyl are each optionally substituted with1, 2, 3, or 4 independently selected R^(1B) substituents;

each R^(a11), R^(c11), and R^(d11) is independently selected from H,C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₇ cycloalkyl,phenyl, 4-7 membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl,wherein said C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(1B) substituents;

each R^(b11) is independently selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl, which are each optionally substitutedwith 1, 2, 3, or 4 independently selected R^(1B) substituents;

each R^(1B) is independently selected from halo, CN, NO₂, C₁₋₆ alkyl,C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄alkyl, phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, 5-6membered heteroaryl-C₁₋₄ alkyl, OR^(a12), SR^(a12), C(O)R^(b12),C(O)NR^(c12)R^(d12), C(O)OR^(a12), OC(O)R^(b12), OC(O)NR^(c12)R^(d12),NR^(c12)R^(d12), NR^(c12)C(O)R^(b12), NR^(c12)C(O)OR^(a12),NR^(c12)C(O)NR^(c12)R^(d12), NR^(c12)S(O)₂R^(b12),NR^(c12)S(O)₂NR^(c12)R^(d12), S(O)₂R^(b12), and S(O)₂NR^(c12)R^(d12);

each R^(a12), R^(c12) and R^(d12) is independently selected from H, C₁₋₆alkyl, and C₁₋₆ haloalkyl; and

each R^(b12) is independently selected from C₁₋₆ alkyl and C₁₋₆haloalkyl.

In some embodiments:

each R^(a1), R^(c1), and R^(d1) is independently selected from H, C₁₋₆alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl, wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(1A) substituents;

each R^(b1) is independently selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl,which are each optionally substituted with 1, 2, 3, or 4 independentlyselected R^(1A) substituents;

each R^(1A) is independently selected from halo, CN, NO₂, C₁₋₆ alkyl,C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl,5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl,4-7 membered heterocycloalkyl-C₁₋₄ alkyl, 5-6 membered heteroaryl-C₁₋₄alkyl, OR^(a11), SR^(a11), C(O)R^(b11), C(O)NR^(c11)R^(d11),C(O)OR^(a11), OC(O)R^(b11), OC(O)NR^(c11)R^(d11), NR^(c11)R^(d11),NR^(c11)C(O)R^(b11), NR^(c11)C(O)OR^(a11), NR^(c11)C(O)NR^(c11)R^(d11),NR^(c11)S(O)₂R^(b11), NR^(c11)S(O)₂NR^(c11)R^(d11), S(O)₂R^(b11), andS(O)₂NR^(c11)R^(d11), wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(1B) substituents;

each R^(a11), R^(c11), and R^(d11) is independently selected from H,C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl, wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(1B) substituents;

each R^(b11) is independently selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl,which are each optionally substituted with 1, 2, 3, or 4 independentlyselected R^(1B) substituents;

each R^(1B) is independently selected from halo, CN, NO₂, C₁₋₆ alkyl,C₁₋₆ haloalkyl, C₃₋₄ cycloalkyl, 4-5 membered heterocycloalkyl,OR^(a12), and NR^(c12)R^(d12); and

each R^(a12), R^(c12), and R^(d12) is independently selected from H,C₁₋₆ alkyl, and C₁₋₆ haloalkyl.

In some embodiments:

each R^(a1), R^(c1), and R^(d1) is independently selected from H, C₁₋₆alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl, wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(1A) substituents;

each R^(b1) is independently selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl,which are each optionally substituted with 1, 2, 3, or 4 independentlyselected R^(1A) substituents;

each R^(1A) is independently selected from halo, CN, NO₂, C₁₋₆ alkyl,C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl,5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl,4-7 membered heterocycloalkyl-C₁₋₄ alkyl, 5-6 membered heteroaryl-C₁₋₄alkyl, OR^(a11), SR^(a11), C(O)R^(b11), C(O)NR^(c11)R^(d11),C(O)OR^(a11), OC(O)R^(b11), OC(O)NR^(c11)R^(d11), NR^(c11)R^(d11),NR^(c11)C(O)R^(b11), NR^(c11)C(O)OR^(a11), NR^(c11)C(O)NR^(c11)R^(d11),NR^(c11)S(O)₂R^(b11), NR^(c11)S(O)₂NR^(c11)R^(d11), S(O)₂R^(b11), andS(O)₂NR^(c11)R^(d11);

each R^(a11), R^(c11), and R^(d11) is independently selected from H,C₁₋₆ alkyl and C₁₋₆ haloalkyl; and

each R^(b11) is independently selected from C₁₋₆ alkyl and C₁₋₆haloalkyl.

In some embodiments:

each R^(a1), R^(c1), and R^(d1) is independently selected from H, C₁₋₆alkyl, and C₁₋₆ haloalkyl, wherein said C₁₋₆ alkyl and C₁₋₆ haloalkylare each optionally substituted with 1, 2, 3, or 4 independentlyselected R^(1A) substituents;

each R^(b1) is independently selected from C₁₋₆ alkyl and C₁₋₆haloalkyl, which are each optionally substituted with 1, 2, 3, or 4independently selected R^(1A) substituents;

each R^(1A) is independently selected from halo, CN, C₁₋₆ alkyl, C₁₋₆haloalkyl, C₃₋₇ cycloalkyl, OR^(a1), and NR^(c11)R^(d11); and

each R^(a11), R^(c11), and R^(d11) is independently selected from H,C₁₋₆ alkyl and C₁₋₆ haloalkyl.

In some embodiments, R² is selected from H, halo, CN, NO₂, C₁₋₆ alkyl,C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₁₋₆ haloalkyl, C₃₋₁₀ cycloalkyl, 6-10membered aryl, 4-10 membered heterocycloalkyl, 5-10 membered heteroaryl,C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10membered heterocycloalkyl-C₁₋₄ alkyl, 5-10 membered heteroaryl-C₁₋₄alkyl, OR^(a2), SR^(a2), NOR^(a2), C(O)R^(b2), C(O)NR^(c2)R^(d2),C(O)OR^(a2), OC(O)R^(b2), OC(O)NR^(c2)R^(d2), NR^(c2)R^(d2),NR^(c2)C(O)R^(b2), NR^(c2)C(O)OR^(a2), NR^(c2)C(O)NR^(c2)R^(d2),NR^(c2)S(O)₂R^(b2), NR^(c2)S(O)₂NR^(c2)R^(d2), S(O)₂R^(b2), andS(O)₂NR^(c2)R^(d2); wherein said C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl,C₁₋₆ haloalkyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10 memberedheterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl,6-10 membered aryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl are each optionallysubstituted by 1, 2, 3, or 4 independently selected R^(2A) substituents.

In some embodiments, R² is selected from H, halo, CN, C₁₋₆ alkyl, C₁₋₆haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7membered heterocycloalkyl-C₁₋₄ alkyl, 5-6 membered heteroaryl-C₁₋₄alkyl, OR^(a2), SR^(a2), C(O)R^(b2), C(O)NR^(c2)R^(d2), C(O)OR^(a2),OC(O)R^(b2), OC(O)NR^(c2)R^(d2), NR^(c2)R^(d2), NR^(c2)C(O)R^(b2),NR^(c2)C(O)OR^(a2), NR^(c2)C(O)NR^(c2)R^(d2), NR^(c2)S(O)₂R^(b2),NR^(c2)S(O)₂NR^(c2)R^(d2), S(O)₂R^(b2), and S(O)₂NR^(c2)R^(d2); whereinsaid C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl are each optionally substituted by 1, 2,3, or 4 independently selected R^(2A) substituents.

In some embodiments, R² is selected from H, halo, CN, C₁₋₆ alkyl, C₁₋₆haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6membered heteroaryl, OR^(a2), C(O)R^(b2), C(O)NR^(c2)R^(d2),C(O)OR^(a2), OC(O)R^(b2), OC(O)NR^(c2)R^(d2), NR^(c2)R^(d2),NR^(c2)C(O)R^(b2), NR^(c2)S(O)₂R^(b2), S(O)₂R^(b2), andS(O)₂NR^(c2)R^(d2); wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, and 5-6 memberedheteroaryl are each optionally substituted by 1, 2, or 3 independentlyselected R^(2A) substituents.

In some embodiments, R² is selected from H, halo, CN, C₁₋₆ alkyl, C₁₋₆haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄alkyl; wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl,4-7 membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted by 1, 2, 3, or 4 independently selectedR^(2A) substituents.

In some embodiments, R² is selected from H, halo, CN, C₁₋₆ alkyl, C₁₋₆haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, and5-6 membered heteroaryl;

wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, and 5-6 membered heteroaryl are eachoptionally substituted by 1, 2, or 3 independently selected R^(2A)substituents.

In some embodiments, R² is selected from H, halo, CN, C₁₋₆ alkyl, C₁₋₆haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, and5-6 membered heteroaryl.

In some embodiments, R² is selected from H, C₁₋₆ alkyl, and C₃₋₇cycloalkyl.

In some embodiments, R² is selected from H, methyl, isopropyl, andcyclopropyl.

In some embodiments, R^(2x) is selected from H, C₁₋₆ alkyl, C₁₋₆haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄alkyl, wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl,4-7 membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted by 1, 2, or 3 independently selected R^(2A)substituents.

In some embodiments, R^(2x) is selected from H, C₁₋₆ alkyl, C₁₋₆haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, and5-6 membered heteroaryl, wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, and 5-6 memberedheteroaryl are each optionally substituted by 1, 2, or 3 independentlyselected R^(2A) substituents.

In some embodiments, R^(2x) is selected from H, C₁₋₆ alkyl, C₁₋₆haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄alkyl.

In some embodiments, R^(2x) is selected from H, C₁₋₆ alkyl, C₃₋₇cycloalkyl, and phenyl-C₁₋₃ alkyl.

In some embodiments, R^(2x) is selected from H, methyl, isopropyl,cyclopropyl, and benzyl.

In some embodiments:

each R^(a2), R^(c2), and R^(d2) is independently selected from H, C₁₋₆alkyl, C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀ cycloalkyl,6-10 membered aryl, 4-10 membered heterocycloalkyl, 5-10 memberedheteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl,4-10 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-10 memberedheteroaryl-C₁₋₄ alkyl, wherein said C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆alkynyl, C₁₋₆ haloalkyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10membered heterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(2A) substituents;

each R^(b2) is independently selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10membered heterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl,which are each optionally substituted with 1, 2, 3, or 4 independentlyselected R^(2A) substituents;

each R^(2A) is independently selected from halo, CN, NO₂, C₁₋₆ alkyl,C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄alkyl, phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, 5-6membered heteroaryl-C₁₋₄ alkyl, OR^(a21), SR^(a21), C(O)R^(b21),C(O)NR^(c21)R^(d21), C(O)OR^(a21), OC(O)R^(b21), OC(O)NR^(c21)R^(d21),NR^(c21)R^(d21), NR^(c21)C(O)R^(b21), NR^(c21)C(O)OR^(a21),NR^(c21)C(O)NR^(c21)R^(d21), NR^(c21)S(O)₂R^(b21),NR^(c21)S(O)₂NR^(c21)R^(d21), S(O)₂R^(b21), and S(O)₂NR^(c21)R^(d21),wherein said C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl, and5-10 membered heteroaryl-C₁₋₄ alkyl are each optionally substituted with1, 2, 3, or 4 independently selected R^(2B) substituents;

each R^(a21), R^(c21), and R^(d21) is independently selected from H,C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₇ cycloalkyl,phenyl, 4-7 membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl,wherein said C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(2B) substituents;

each R^(b21) is independently selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl, which are each optionally substitutedwith 1, 2, 3, or 4 independently selected R^(2B) substituents;

each R^(2B) is independently selected from halo, CN, NO₂, C₁₋₆ alkyl,C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄alkyl, phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, 5-6membered heteroaryl-C₁₋₄ alkyl, OR^(a22), SR^(a22), C(O)R^(b22),C(O)NR^(c22)R^(d22), C(O)OR^(a22), OC(O)R^(b22), OC(O)NR^(c22)R^(d22),NR^(c22)R^(d22), NR^(c22)C(O)R^(b22), NR^(c22)C(O)OR^(a22),NR^(c22)C(O)NR^(c22)R^(d22), NR^(c22)S(O)₂R^(b22),NR^(c22)S(O)₂NR^(c22)R^(d22), S(O)₂R^(b22), and S(O)₂NR^(c22)R^(d22);

each R^(a22), R^(c22), and R^(d22) is independently selected from H,C₁₋₆ alkyl, and C₁₋₆ haloalkyl; and

each R^(b22) is independently selected from C₁₋₆ alkyl and C₁₋₆haloalkyl.

In some embodiments:

each R^(a2), R^(c2), and R^(d2) is independently selected from H, C₁₋₆alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl, wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(2A) substituents;

each R^(b2) is independently selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl,which are each optionally substituted with 1, 2, 3, or 4 independentlyselected R^(2A) substituents;

each R^(2A) is independently selected from halo, CN, NO₂, C₁₋₆ alkyl,C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl,5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl,4-7 membered heterocycloalkyl-C₁₋₄ alkyl, 5-6 membered heteroaryl-C₁₋₄alkyl, OR^(a21), SR^(a21), C(O)R^(b21), C(O)NR^(c21)R^(d21),C(O)OR^(a21), OC(O)R^(b21), OC(O)NR^(c21)R^(d21), NR^(c21)R^(d21),NR^(c21)C(O)R^(b21), NR^(c21)C(O)OR^(a21), NR²¹C(O)NR^(c21)R^(d21),NR^(c21)S(O)₂R^(b21), NR^(c21)S(O)₂NR^(c21)R^(d21), S(O)₂R^(b21), andS(O)₂NR^(c21)R^(d21), wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(2B) substituents;

each R^(a21), R^(c21), and R^(d21) is independently selected from H,C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl, wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(2B) substituents;

each R^(b21) is independently selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl,which are each optionally substituted with 1, 2, 3, or 4 independentlyselected R^(2B) substituents;

each R^(2B) is independently selected from halo, CN, NO₂, C₁₋₆ alkyl,C₁₋₆ haloalkyl, C₃₋₄ cycloalkyl, 4-5 membered heterocycloalkyl,OR^(a22), and NR^(c22)R^(d22); and

each R^(a22), R^(c22), and R^(d22) is independently selected from H,C₁₋₆ alkyl, and C₁₋₆ haloalkyl.

In some embodiments:

each R^(a2), R^(c2), and R^(d2) is independently selected from H, C₁₋₆alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl, wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(2A) substituents;

each R^(b2) is independently selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl,which are each optionally substituted with 1, 2, 3, or 4 independentlyselected R^(2A) substituents;

each R^(2A) is independently selected from halo, CN, NO₂, C₁₋₆ alkyl,C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl,5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl,4-7 membered heterocycloalkyl-C₁₋₄ alkyl, 5-6 membered heteroaryl-C₁₋₄alkyl, OR^(a21), SR^(a21), C(O)R^(b21), C(O)NR^(c21)R^(d21),C(O)OR^(a21), OC(O)R^(b21), OC(O)NR^(c21)R^(d21), NR^(c21)R^(d21),NR^(c21)C(O)R^(b21), NR^(c21)C(O)OR^(a21), NR²¹C(O)NR^(c21)R^(d21),NR^(c21)S(O)₂R^(b21), NR^(c21)S(O)₂NR^(c21)R^(d21), S(O)₂R^(b21), andS(O)₂NR^(c21)R^(d21).

each R^(a21), R^(c21), and R^(d21) is independently selected from H,C₁₋₆ alkyl and C₁₋₆ haloalkyl; and

each R^(b21) is independently selected from C₁₋₆ alkyl and C₁₋₆haloalkyl.

In some embodiments:

each R^(a2), R^(c2), and R^(d2) is independently selected from H, C₁₋₆alkyl, and C₁₋₆ haloalkyl, wherein said C₁₋₆ alkyl and C₁₋₆ haloalkylare each optionally substituted with 1, 2, 3, or 4 independentlyselected R^(2A) substituents;

each R^(b2) is independently selected from C₁₋₆ alkyl and C₁₋₆haloalkyl, which are each optionally substituted with 1, 2, 3, or 4independently selected R^(2A) substituents;

each R^(2A) is independently selected from halo, CN, NO₂, C₁₋₆ alkyl,C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, OR^(a21),and NR^(c21)R^(d21); and

each R^(a21), R^(c21), and R^(d21) is independently selected from H,C₁₋₆ alkyl and C₁₋₆ haloalkyl.

In some embodiments, R³ is selected from H, halo, CN, C₁₋₃ alkyl, andC₁₋₃ haloalkyl.

In some embodiments, R³ is selected from H, F, CH₃, and CF₃.

In some embodiments, R³ is H.

In some embodiments, Y is N.

In some embodiments, Y is CR⁷.

In some embodiments, R⁷ is selected from H, halo, CN, C₁₋₃ alkyl, andC₁₋₃ haloalkyl.

In some embodiments, R⁷ is selected from H, F, CH₃, and CF₃.

In some embodiments, R⁷ is H.

In some embodiments, Z¹ is CR⁴R⁵; and Z² is CR^(4z)R^(5z) or O.

In some embodiments, Z¹ is CH₂; and Z² is CH₂ or O.

In some embodiments, Z¹ is CR⁴R⁵ or O; and Z² is CR^(4z)R^(5z).

In some embodiments, Z¹ is CH₂ or O; and Z² is CH₂.

In some embodiments:

R⁴, R^(4z), R⁵ and R^(5z) are independently selected from H, halo, CN,C₁₋₃ alkyl, and C₁₋₃ haloalkyl;

or R⁴ and R⁵, together with the carbon atom to which they are attached,form a cyclopropyl or cyclobutyl ring;

or R^(4z) and R^(5z), together with the carbon atom to which they areattached, form a 3 cyclopropyl or cyclobutyl ring.

In some embodiments, R⁴, R^(4z), R⁵ and R^(5z) are independentlyselected from H, F, CH₃ and CF₃; or R⁴ and R⁵, together with the carbonatom to which they are attached, form a cyclopropyl ring.

In some embodiments, R⁴, R^(4z), R⁵ and R^(5z) are each independentlyselected from H and C₁₋₃ alkyl.

In some embodiments, R⁴, R^(4z), R⁵ and R^(5z) are each H.

In some embodiments, R⁶ and R^(6z) are each independently selected fromH, C₁₋₃ alkyl, and C₁₋₃ haloalkyl.

In some embodiments, R⁶ and R^(6z) are each independently selected fromH, CH₃, and CF₃.

In some embodiments, Ring moiety A is monocyclic or bicyclic 4-10membered heterocycloalkyl having 1, 2, 3, or 4 ring-forming heteroatomsindependently selected from N, O, and S, wherein 1, 2, 3, or 4ring-forming carbon or heteroatoms can be optionally substituted by oneor more oxo or sulfido, wherein said heterocycloalkyl does not comprisean aromatic ring.

In some embodiments, Ring moiety A is monocyclic C₃₋₇ cycloalkyl.

In some embodiments, Ring moiety A is monocyclic 4-7 memberedheterocycloalkyl having 1 or 2 ring-forming heteroatoms independentlyselected from N, O, and S, wherein 1, 2 or 3 ring-forming carbon orheteroatoms can be optionally substituted by one or more oxo or sulfido.

In some embodiments, Ring moiety A is an azetidine ring, a pyrrolidinering, a piperidine ring, or an azepane ring.

In some embodiments, Ring moiety A is azetidin-3-yl, piperidin-3-yl, orpiperidin-4-yl.

In some embodiments, Ring moiety A is a piperidine ring.

In some embodiments, Ring moiety A is piperidin-4-yl.

In some embodiments, n is 1, 2, 3, or 4.

In some embodiments, n is 1, 2, or 3.

In some embodiments, n is 1 or 2.

In some embodiments, n is 2.

In some embodiments, n is 1.

In some embodiments, R⁸ is selected from H, halo, CN, NO₂, C₁₋₆ alkyl,C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₁₋₆ haloalkyl, C₃₋₁₀ cycloalkyl, 6-10membered aryl, 4-10 membered heterocycloalkyl, 5-10 membered heteroaryl,C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10membered heterocycloalkyl-C₁₋₄ alkyl, 5-10 membered heteroaryl-C₁₋₄alkyl, OR^(a8), SR^(a8), NHOR^(a8), C(O)R^(b8), C(O)NR^(c8)R^(d8),C(O)OR^(a8), OC(O)R^(b8), OC(O)NR^(c8)R^(d8), NR^(c8)R^(d8),NR^(c8)C(O)R^(b8), NR^(c8)C(O)OR^(a8), NR^(c8)C(O)NR^(c8)R^(d8),NR^(c8)S(O)₂R^(b8), NR^(c8)S(O)₂NR^(c8)R^(d8), S(O)₂R^(b8), andS(O)₂NR^(c8)R^(d8); wherein said C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl,C₁₋₆ haloalkyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10 memberedheterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀cycloalkyl-C₁₋₄ alkyl,6-10 membered aryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl are each optionallysubstituted by 1, 2, 3, or 4 independently selected R^(8A) substituents.

In some embodiments, R⁸ is selected from H, halo, CN, C₁₋₆ alkyl, C₁₋₆haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7membered heterocycloalkyl-C₁₋₄ alkyl, 5-6 membered heteroaryl-C₁₋₄alkyl, OR^(a8), SR^(a8), C(O)R^(b8), C(O)NR^(c8)R^(d8), C(O)OR^(a8),OC(O)R^(b8), OC(O)NR^(c8)R^(d8), NR^(c8)R^(d8), NR^(c8)C(O)R^(b8),NR^(c8)C(O)OR^(a8), NR^(c8)C(O)NR^(c8)R^(d8), NR^(c8)S(O)₂R^(b8),NR^(c8)S(O)₂NR^(c8)R^(d8), S(O)₂R^(b8), and S(O)₂NR^(c8)R^(d8); whereinsaid C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl are each optionally substituted by 1, 2,3, or 4 independently selected R^(8A) substituents.

In some embodiments, at least one R⁸ is S(O)₂R^(b8).

In some embodiments, one R⁸ is S(O)₂R^(b8); and each remaining R⁸ isindependently selected from H, halo, CN, C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, 5-6 membered heteroaryl-C₁₋₄ alkyl,OR^(a8), SR^(a8), C(O)R^(b8), C(O)NR^(c8)R^(d8), C(O)OR^(a8),OC(O)R^(b8), OC(O)NR^(c8)R^(d8), NR^(c8)R^(d8), NR^(c8)C(O)R^(b8),NR^(c8)C(O)OR^(a8), NR^(c8)C(O)NR^(c8)CR^(d8), NR^(c8)S(O)₂R^(b8),NR^(c8)S(O)₂NR^(c8)R^(d8), S(O)₂R^(b8), and S(O)₂NR^(c8)R^(d8); whereinsaid C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl are each optionally substituted by 1, 2,3, or 4 independently selected R^(8A) substituents.

In some embodiments, one R⁸ is S(O)₂R^(b8); and each remaining R⁸ isindependently selected from halo, CN, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl,OR^(a8), and NR^(c8)R^(d8); wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted by 1, 2, 3, or 4 independently selectedR^(8A) substituents.

In some embodiments, one R⁸ is S(O)₂R^(b8); and each remaining R⁸ isindependently selected from halo, CN, C₁₋₆ alkyl, C₁₋₆ haloalkyl,OR^(a8), and NR^(c8)R^(d8); wherein said C₁₋₆ alkyl and C₁₋₆ haloalkylare each optionally substituted by 1, 2, 3, or 4 independently selectedR^(8A) substituents.

In some embodiments, one R⁸ is S(O)₂R^(b8); and each remaining R⁸ isindependently selected from C₁₋₆ alkyl and C₁₋₆ haloalkyl.

In some embodiments, one R⁸ is S(O)₂R^(b8), wherein R^(b8) isindependently selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl,phenyl, 4-7 membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl,which are each optionally substituted with 1, 2, 3, or 4 independentlyselected R^(8A) substituents.

In some embodiments, one R⁸ is halo or C₁₋₆ alkyl and one R⁸ isS(O)₂R^(b8), wherein R^(b8) is selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, and 5-6 memberedheteroaryl, each of which is optionally substituted by 1, 2, 3, or 4independently selected R^(8A) substituents.

In some embodiments, one R⁸ is S(O)₂R^(b8), wherein R^(b8) isindependently selected from selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, and 5-6 memberedheteroaryl, each of which is optionally substituted by 1, 2, 3, or 4independently selected R^(8A) substituents.

In some embodiments, one R⁸ is S(O)₂R^(b8), wherein R^(b8) is selectedfrom methyl, ethyl, cyclopropyl, 3-cyanophenyl, pyrazol-4-yl,1,2-dimethyl-1H-imidazol-4-yl, 1-methyl-1H-imidazol-4-yl,6-(4-methylpiperazin-1-yl)pyridin-2-yl,2-[(3-hydroxy)pyrrolidinyl]-pyridin-5-yl, and 1-methyl-pyrazol-4-yl.

In some embodiments, one R⁸ is S(O)₂R^(b8), wherein R^(b8) is selectedfrom methyl, ethyl, cyclopropyl, 3-cyanophenyl, pyrazol-4-yl,1,2-dimethyl-1H-imidazol-4-yl and 1-methyl-pyrazol-4-yl.

In some embodiments, one R⁸ is halo or C₁₋₆ alkyl and one R⁸ isS(O)₂R^(b8), wherein R^(b8) is selected from methyl, ethyl, cyclopropyl,3-cyanophenyl, pyrazol-4-yl, 1,2-dimethyl-1H-imidazol-4-yl,1-methyl-1H-imidazol-4-yl, 6-(4-methylpiperazin-1-yl)pyridin-2-yl,2-[(3-hydroxy)pyrrolidinyl]-pyridin-5-yl, and 1-methyl-pyrazol-4-yl.

In some embodiments, one R is C₁₋₆ alkyl and one R⁸ is S(O)₂R^(b8),wherein R^(b8) is selected from methyl, ethyl, cyclopropyl,3-cyanophenyl, pyrazol-4-yl, 1,2-dimethyl-1H-imidazol-4-yl,1-methyl-1H-imidazol-4-yl, 6-(4-methylpiperazin-1-yl)pyridin-2-yl,2-[(3-hydroxy)pyrrolidinyl]-pyridin-5-yl, and 1-methyl-pyrazol-4-yl.

In some embodiments, one R⁸ is methyl and one R⁸ is S(O)₂R^(b8), whereinR^(b8) is selected from methyl, ethyl, cyclopropyl, 3-cyanophenyl,pyrazol-4-yl, 1,2-dimethyl-1H-imidazol-4-yl, 1-methyl-1H-imidazol-4-yl,6-(4-methylpiperazin-1-yl)pyridin-2-yl,2-[(3-hydroxy)pyrrolidinyl]-pyridin-5-yl, and 1-methyl-pyrazol-4-yl.

In some embodiments:

each R^(a8), R^(c8), and R^(d8) is independently selected from H, C₁₋₆alkyl, C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀ cycloalkyl,6-10 membered aryl, 4-10 membered heterocycloalkyl, 5-10 memberedheteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl,4-10 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-10 memberedheteroaryl-C₁₋₄ alkyl, wherein said C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆alkynyl, C₁₋₆ haloalkyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10membered heterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(8A) substituents;

each R^(b8) is independently selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10membered heterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl,which are each optionally substituted with 1, 2, 3, or 4 independentlyselected R^(8A) substituents;

each R^(8A) is independently selected from halo, CN, NO₂, C₁₋₆ alkyl,C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄alkyl, phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, 5-6membered heteroaryl-C₁₋₄ alkyl, OR^(a81), SR^(a81), C(O)R^(b81),C(O)NR^(c81)R^(d81), C(O)OR^(a81), OC(O)R^(a81), OC(O)NR^(c81)R^(d81),NR^(c81)R^(d81), NR^(c81)C(O)R^(b81), NR^(c81)C(O)OR^(a81),NR^(c81)C(O)NR^(c81)R^(d81), NR^(c81)S(O)₂R^(b81),NR^(c81)S(O)₂NR^(c81)R^(d81), S(O)₂R^(b81), and S(O)₂NR^(c81)R^(d81),wherein said C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(8B) substituents;

each R^(a81), R^(c81), and R^(d81) is independently selected from H,C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₇ cycloalkyl,phenyl, 4-7 membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl,wherein said C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(8B) substituents;

each R^(b81) is independently selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl, which are each optionally substitutedwith 1, 2, 3, or 4 independently selected R^(8B) substituents;

each R^(8B) is independently selected from halo, CN, NO₂, C₁₋₆ alkyl,C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄alkyl, phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, 5-6membered heteroaryl-C₁₋₄ alkyl, OR^(a82), SR^(a82), C(O)R^(b82),C(O)NR^(c82)R^(d82), C(O)OR^(a82), OC(O)R^(b82), OC(O)NR^(c82)R^(d82),NR^(c82)R^(d82), NR^(c82)C(O)R^(b82), NR^(c82)C(O)OR^(a82),NR^(c82)C(O)NR^(c82)R^(d82), NR^(c82)S(O)₂R^(b82),NR^(c82)S(O)₂NR^(c82)R^(d82), S(O)₂R^(b82), and S(O)₂NR^(c82)R^(d82);

each R^(a82), R^(c82), and R^(d82) is independently selected from H,C₁₋₆ alkyl, and C₁₋₆ haloalkyl; and

each R^(b82) is independently selected from C₁₋₆ alkyl and C₁₋₆haloalkyl.

In some embodiments:

each R^(a8), R^(c8), and R^(d8) is independently selected from H, C₁₋₆alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl, wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(8A) substituents;

each R^(b8) is independently selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl,which are each optionally substituted with 1, 2, 3, or 4 independentlyselected R^(8A) substituents;

each R^(8A) is independently selected from halo, CN, NO₂, C₁₋₆ alkyl,C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl,5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl,4-7 membered heterocycloalkyl-C₁₋₄ alkyl, 5-6 membered heteroaryl-C₁₋₄alkyl, OR^(a81), SR^(a81), C(O)R^(b81), C(O)NR^(c81)R^(d81),C(O)OR^(a81), OC(O)R^(a81), OC(O)NR^(c81)R^(d81), NR^(c81)R^(d81),NR^(c81)C(O)R^(b81), NR^(c11)C(O)OR^(a81), R^(c81)C(O)NR^(c81)R^(d81),NR^(c81)S(O)₂R^(b81), NR^(c81)S(O)₂NR^(c81)R^(d81), S(O)₂R^(b81), andS(O)₂NR^(c81)R^(d81), wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selected RBsubstituents;

each R^(a81), R^(c81), and R^(d81) is independently selected from H,C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl, wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(8B) substituents;

each R^(b81) is independently selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl,which are each optionally substituted with 1, 2, 3, or 4 independentlyselected R^(8B) substituents;

each R^(8B) is independently selected from halo, CN, NO₂, C₁₋₆ alkyl,C₁₋₆ haloalkyl, C₃₋₄ cycloalkyl, 4-5 membered heterocycloalkyl,OR^(b82), and NR^(c82)R^(d82); and

each R^(a82), R^(c82), and R^(d82) is independently selected from H,C₁₋₆ alkyl, and C₁₋₆ haloalkyl.

In some embodiments:

each R^(a8), R^(c8), and R^(d8) is independently selected from H, C₁₋₆alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl, wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(8A) substituents;

each R^(b8) is independently selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl,which are each optionally substituted with 1, 2, 3, or 4 independentlyselected R^(8A) substituents;

each R^(8A) is independently selected from halo, CN, NO₂, C₁₋₆ alkyl,C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl,5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl,4-7 membered heterocycloalkyl-C₁₋₄ alkyl, 5-6 membered heteroaryl-C₁₋₄alkyl, OR^(a81), SR^(a81), C(O)R^(b81), C(O)NR^(c81)R^(d81),C(O)OR^(a81), OC(O)R^(a81), OC(O)NR^(c81)R^(d81), NR^(c81)R^(d81),NR^(c81)C(O)R^(b81), NR^(c81)C(O)OR^(a81) NR^(c81)C(O)NR^(c81)R^(d81),NR^(c81)S(O)₂R^(b81), NR^(c81)S(O)₂NR^(c81)R^(d81), S(O)₂R^(b811), andS(O)₂NR^(c81)R^(d81);

each R^(a81), R^(c81), and R^(d81) is independently selected from H,C₁₋₆ alkyl and C₁₋₆ haloalkyl; and

each R^(b81) is independently selected from C₁₋₆ alkyl and C₁₋₆haloalkyl.

In some embodiments:

each R^(8A) is independently selected from halo, CN, C₁₋₆ alkyl, C₁₋₆haloalkyl, and 4-7 membered heterocycloalkyl; wherein said C₁₋₆ alkyl,C₁₋₆ haloalkyl, and 4-7 membered heterocycloalkyl are each optionallysubstituted by 1 or 2 independently selected R^(8B) substituents;

each R^(8B) is selected from C₁₋₆ alkyl and OR^(a82); and

each R^(a82) is independently selected from Hand C₁₋₆ alkyl.

In some embodiments:

each R^(a8), R^(c8), and R^(d8) is independently selected from H, C₁₋₆alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl, wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(8A) substituents;

each R^(b8) is independently selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl,which are each optionally substituted with 1, 2, 3, or 4 independentlyselected R^(8A) substituents;

each R^(8A) is independently selected from halo, CN, NO₂, C₁₋₆ alkyl,C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₁₋₆ haloalkyl, C₃₋₄ cycloalkyl, OR^(a81),and NR^(c81)R^(d81); and

each R^(a81), R^(c81), and R^(d81) is independently selected from H,C₁₋₆ alkyl and C₁₋₆ haloalkyl.

In some embodiments:

n is 1, 2, 3, or 4;

Z¹ is CR⁴R⁵; and Z² is NR^(6z), O, or S; or

Z¹ is CR⁴R⁵, NR⁶, O, or S; and Z² is CR^(4z)R^(5z);

Y is CR⁷ or N;

X¹ is NR^(1x) or CR¹;

X² is NR^(2x) or CR²;

X³ is N or C;

wherein Ring

is an aromatic ring selected from:

Ring moiety A is monocyclic or bicyclic 4-10 membered heterocycloalkylhaving 1, 2, 3, or 4 ring-forming heteroatoms independently selectedfrom N, O, and S, wherein 1, 2, 3, or 4 ring-forming carbon orheteroatoms can be optionally substituted by one or more oxo or sulfido,wherein said heterocycloalkyl does not comprise an aromatic ring;

R¹ is selected from H, halo, CN, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, 5-6 membered heteroaryl-C₁₋₄ alkyl,OR^(a1), SR^(a1), C(O)R^(b1), C(O)NR^(c1)R^(d1), C(O)OR^(a1),OC(O)R^(b1), OC(O)NR^(c1)R^(d1), NR^(c1)R^(d1), NR^(c1)C(O)R^(b1),NR^(c1)C(O)OR^(a1), NR^(c1)C(O)NR^(c1)R^(d1), NR^(c1)S(O)₂R^(b1),NR^(c1)S(O)₂NR^(c1)R^(d1), S(O)₂R^(b1), and S(O)₂NR^(c1)R^(d1); whereinsaid C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl are each optionally substituted by 1, 2,3, or 4 independently selected R^(1A) substituents;

R^(1x) is selected from H, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl,phenyl, 4-7 membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl;wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄alkyl, phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and5-6 membered heteroaryl-C₁₋₄ alkyl are each optionally substituted by 1,2, or 3 independently selected R^(1A) substituents;

R² is selected from H, halo, CN, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, 5-6 membered heteroaryl-C₁₋₄ alkyl,OR^(a2), SR^(a2), C(O)R^(b2), C(O)NR^(c2)R^(d2), C(O)OR^(a2),OC(O)R^(b2), OC(O)NR^(c2)R^(d2), NR^(c2)R^(d2), NR^(c2)C(O)R^(b2),NR^(c2)C(O)OR^(a2), NR^(c2)C(O)NR^(c2)R^(d2), NR^(c2)S(O)₂R^(b2),NR^(c2)S(O)₂NR^(c2)R^(d2), S(O)₂R^(b2), and S(O)₂NR^(c2)R^(d2); whereinsaid C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl are each optionally substituted by 1, 2,3, or 4 independently selected R^(2A) substituents;

R^(2x) is selected from H, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl,phenyl, 4-7 membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl,wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄alkyl, phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and5-6 membered heteroaryl-C₁₋₄ alkyl are each optionally substituted by 1,2, or 3 independently selected R^(2A) substituents;

R³ is selected from H, halo, CN, C₁₋₃ alkyl, and C₁₋₃ haloalkyl;

R⁴, R^(4z), R⁵ and R^(5z) are independently selected from H, halo, CN,C₁₋₃ alkyl, and C₁₋₃ haloalkyl;

or R⁴ and R⁵, together with the carbon atom to which they are attached,form a cyclopropyl or cyclobutyl ring;

or R^(4z) and R^(5z), together with the carbon atom to which they areattached, form a 3 cyclopropyl or cyclobutyl ring;

R⁷ is selected from H, halo, CN, C₁₋₃ alkyl, and C₁₋₃ haloalkyl;

R⁸ is selected from H, halo, CN, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, 5-6 membered heteroaryl-C₁₋₄ alkyl,OR^(a8), SR^(a8), C(O)R^(b8), C(O)NR^(c8)R^(d8), C(O)OR^(a8),OC(O)R^(b8), OC(O)NR^(c8)R^(d8), NR^(c8)R^(d8), NR^(c8)C(O)R^(b8),NR^(c8)C(O)OR^(a8), NR^(c8)C(O)NR^(c8)R^(d8), NR^(c8)S(O)₂R^(b8),NR^(c8)S(O)₂NR^(c8)R^(d8), S(O)₂R^(b8), and S(O)₂NR^(c8)R^(d8); whereinsaid C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl are each optionally substituted by 1, 2,3, or 4 independently selected R^(8A) substituents;

each R^(a1), R^(c1), and R^(d1) is independently selected from H, C₁₋₆alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl, wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(1A) substituents;

each R^(b1) is independently selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl,which are each optionally substituted with 1, 2, 3, or 4 independentlyselected R^(1A) substituents;

each R^(1A) is independently selected from halo, CN, NO₂, C₁₋₆ alkyl,C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl,5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl,4-7 membered heterocycloalkyl-C₁₋₄ alkyl, 5-6 membered heteroaryl-C₁₋₄alkyl, OR^(a11), SR^(a11), C(O)R^(b11), C(O)NR^(c11)R^(d11),C(O)OR^(a11), OC(O)R^(b11), OC(O)NR^(c11)R^(d11), NR^(c11)R^(d11),NR^(c11)C(O)R^(b11), NR^(c11)C(O)OR^(a11), NR^(c11)C(O)NR^(c11)R^(d11),NR^(c11)S(O)₂R^(b11), NR^(c11)S(O)₂NR^(c11)R^(d11), S(O)₂R^(b11), andS(O)₂NR^(c11)R^(d11), wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(1B) substituents;

each R^(a11), R^(c11), and R^(d11) is independently selected from H,C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl, wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(1B) substituents;

each R^(b11) is independently selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl,which are each optionally substituted with 1, 2, 3, or 4 independentlyselected R^(1B) substituents;

each R^(1B) is independently selected from halo, CN, NO₂, C₁₋₆ alkyl,C₁₋₆ haloalkyl, C₃₋₄ cycloalkyl, 4-5 membered heterocycloalkyl,OR^(a12), and NR^(c12)R^(d12);

each R^(a12), R^(c12), and R^(d12) is independently selected from H,C₁₋₆ alkyl, and C₁₋₆ haloalkyl;

each R^(a2), R^(c2), and R^(d2) is independently selected from H, C₁₋₆alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl, wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(2A) substituents;

each R^(b2) is independently selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl,which are each optionally substituted with 1, 2, 3, or 4 independentlyselected R^(2A) substituents;

each R^(2A) is independently selected from halo, CN, NO₂, C₁₋₆ alkyl,C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl,5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl,4-7 membered heterocycloalkyl-C₁₋₄ alkyl, 5-6 membered heteroaryl-C₁₋₄alkyl, OR^(a21), SR^(a21), C(O)R^(b21), C(O)NR^(c21)R^(d21),C(O)OR^(a21), OC(O)R^(b21), OC(O)NR^(c21)R^(d21), NR^(c21)R^(d21),NR^(c21)C(O)R^(b21), NR^(c21)C(O)OR^(a21), NR²¹C(O)NR^(c21)R^(d21),NR^(c21)S(O)₂R^(b21), NR^(c21)S(O)₂NR^(c21)R^(d21), S(O)₂R^(b21), andS(O)₂NR^(c21)R^(d21), wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(2B) substituents;

each R^(a21), R^(c21), and R^(d21) is independently selected from H,C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl, wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(2B) substituents;

each R^(b21) is independently selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl,which are each optionally substituted with 1, 2, 3, or 4 independentlyselected R^(2B) substituents;

each R^(2B) is independently selected from halo, CN, NO₂, C₁₋₆ alkyl,C₁₋₆ haloalkyl, C₃₋₄ cycloalkyl, 4-5 membered heterocycloalkyl,OR^(a22), and NR^(c22)R^(d22) each R^(a22), R^(c22), and R^(d22) isindependently selected from H, C₁₋₆ alkyl, and C₁₋₆ haloalkyl;

each R^(a8), R^(c8), and R^(d8) is independently selected from H, C₁₋₆alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl, wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(8A) substituents;

each R^(b8) is independently selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl,which are each optionally substituted with 1, 2, 3, or 4 independentlyselected R^(8A) substituents;

each R^(8A) is independently selected from halo, CN, NO₂, C₁₋₆ alkyl,C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl,5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl,4-7 membered heterocycloalkyl-C₁₋₄ alkyl, 5-6 membered heteroaryl-C₁₋₄alkyl, OR^(a81), SR^(a81), C(O)R^(b81), C(O)NR^(c81)R^(d81),C(O)OR^(a81), OC(O)R^(a81), OC(O)NR^(c81)R^(d81), NR^(c81)R^(d81),NR^(c81)C(O)R^(b11), NR^(c11)C(O)OR^(a81), NR^(c81)C(O)NR^(c81)R^(d81),NR^(c81)S(O)₂R^(b81), NR^(c81)S(O)₂NR^(c81)R^(d81), S(O)₂R^(b81), andS(O)₂NR^(c81)R^(d81), wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selected RBsubstituents;

each R^(a81), R^(c81), and R^(d81) is independently selected from H,C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl, wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(8B) substituents;

each R^(b81) is independently selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl,which are each optionally substituted with 1, 2, 3, or 4 independentlyselected R^(8B) substituents;

each R^(8B) is independently selected from halo, CN, NO₂, C₁₋₆ alkyl,C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄alkyl, phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, 5-6membered heteroaryl-C₁₋₄ alkyl, OR^(a82), SR^(a82), C(O)R^(b82),C(O)NR^(c82)R^(b82), C(O)OR^(a82), OC(O)R^(b82), OC(O)NR^(c82)R^(d82),NR^(c82)R^(d82), NR^(c82)C(O)R^(b82), NR^(c82)C(O)OR^(a82),NR^(c2)C(O)NR^(c82)R^(d82), NR^(c82)S(O)₂R^(b82),NR^(c82)S(O)₂NR^(c82)R^(d82), S(O)₂R^(b82), and S(O)₂NR^(c82)R^(d82);

each R^(a82), R^(c82), and R^(d82) is independently selected from H,C₁₋₆ alkyl, and C₁₋₆ haloalkyl; and

each R^(b82) is independently selected from C₁₋₆ alkyl and C₁₋₆haloalkyl.

In some embodiments:

n is 1, 2, or 3;

Z¹ is CR⁴R⁵; and Z² is O; or

Z¹ is CR⁴R⁵ or O; and Z² is CR^(4z)R^(5z);

Y is N or CH;

X¹ is NR^(1x) or CR¹;

X² is NR^(2x) or CR²;

X³ is N or C;

wherein Ring

is an aromatic ring selected from:

Ring moiety A is monocyclic 4-7 membered heterocycloalkyl having 1 or 2ring-forming heteroatoms independently selected from N, O, and S,wherein 1, 2, or 3 ring-forming carbon or heteroatoms can be optionallysubstituted by one or more oxo or sulfido;

R¹ is selected from H, halo, CN, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl;wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄alkyl, phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and5-6 membered heteroaryl-C₁₋₄ alkyl are each optionally substituted by 1,2, 3, or 4 independently selected R^(1A) substituents;

R^(1x) is selected from H, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl,phenyl, 4-7 membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl;wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄alkyl, phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and5-6 membered heteroaryl-C₁₋₄ alkyl are each optionally substituted by 1,2, or 3 independently selected R^(1A) substituents;

R² is selected from H, halo, CN, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl;wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄alkyl, phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and5-6 membered heteroaryl-C₁₋₄ alkyl are each optionally substituted by 1,2, 3, or 4 independently selected R^(2A) substituents;

R^(2x) is selected from H, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl,phenyl, 4-7 membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl,wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄alkyl, phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and5-6 membered heteroaryl-C₁₋₄ alkyl are each optionally substituted by 1,2, or 3 independently selected R^(2A) substituents;

R³ is selected from H, F, CH₃, and CF₃;

R⁴, R^(4z), R⁵ and R^(5z) are independently selected from H, F, CH₃ andCF₃; or

R⁴ and R⁵, together with the carbon atom to which they are attached,form a cyclopropyl ring;

at least one R⁸ is S(O)₂RV; and each remaining R⁸ is independentlyselected from halo, CN, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl,phenyl, 4-7 membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, 5-6 membered heteroaryl-C₁₋₄ alkyl,OR^(a8), and NR^(c8)R^(d8); wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted by 1, 2, 3, or 4 independently selectedR^(8A) substituents;

each R^(1A) is independently selected from halo, CN, NO₂, C₁₋₆ alkyl,C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl,5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl,4-7 membered heterocycloalkyl-C₁₋₄ alkyl, 5-6 membered heteroaryl-C₁₋₄alkyl, OR^(a11), SR^(a11), C(O)R^(b1), C(O)NR^(c11)R^(d11),C(O)OR^(a11), OC(O)R^(b11), OC(O)NR^(c11)R^(d11), NR^(c11)R^(d11),NR^(c11)C(O)R^(b11), NR^(c11)C(O)OR^(a11), NR^(c11)C(O)NR^(c11)R^(d11),NR^(c11)S(O)₂R^(b11), NR^(c11)S(O)₂R^(c11)R^(d11), S(O)₂R^(b11), andS(O)₂NR^(c11)R^(d11).

each R^(a11), R^(c11), and R^(d11) is independently selected from H,C₁₋₆ alkyl and C₁₋₆ haloalkyl; and

each R^(b11) is independently selected from C₁₋₆ alkyl and C₁₋₆haloalkyl.

each R^(2A) is independently selected from halo, CN, NO₂, C₁₋₆ alkyl,C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl,5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl,4-7 membered heterocycloalkyl-C₁₋₄ alkyl, 5-6 membered heteroaryl-C₁₋₄alkyl, OR^(a21), SR^(a21), C(O)R^(b21), C(O)NR^(c21)R^(d21),C(O)OR^(a21), OC(O)R^(b21), OC(O)NR^(c21)R^(d21), NR^(c21)R^(d21),NR^(c21)C(O)R^(b21), NR^(c21)C(O)OR^(a21), NR^(c21)C(O)NR^(c21)R^(d21),NR^(c21)S(O)₂R^(b21), NR^(c21)S(O)₂R^(c21)R^(d21), S(O)₂R^(b21), andS(O)₂NR^(c21)R^(d21).

each R^(a21), R²¹, and R^(d21) is independently selected from H, C₁₋₆alkyl and C₁₋₆ haloalkyl;

each R^(b21) is independently selected from C₁₋₆ alkyl and C₁₋₆haloalkyl;

each R^(a8), R^(c8), and R^(d8) is independently selected from H, C₁₋₆alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl, wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(8A) substituents;

each R^(b8) is independently selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl,which are each optionally substituted with 1, 2, 3, or 4 independentlyselected R^(8A) substituents;

each R^(8A) is independently selected from halo, CN, NO₂, C₁₋₆ alkyl,C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl,5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl,4-7 membered heterocycloalkyl-C₁₋₄ alkyl, 5-6 membered heteroaryl-C₁₋₄alkyl, OR^(a81), C(O)R^(a81), C(O)NR^(c81)R^(d81), C(O)OR^(a81),OC(O)R^(b81), NR^(c81)R^(d81), NR^(c81)C(O)R^(b81), S(O)₂R^(b81), andS(O)₂NR^(c81)R^(d81);

each R^(a81), R^(c81), and R^(d81) is independently selected from H,C₁₋₆ alkyl and C₁₋₆ haloalkyl; and

each R^(b81) is independently selected from C₁₋₆ alkyl and C₁₋₆haloalkyl.

In some embodiments:

n is 1 or 2;

Z¹ is CR⁴R⁵; and Z² is O; or

Z¹ is CR⁴R⁵ or O; and Z² is CR^(4z)R^(5z);

Y is N or CH;

X¹ is NR^(1x) or CR¹;

X² is NR^(2x) or CR²;

X³ is N or C;

wherein Ring

is an aromatic ring selected from:

Ring moiety A is an azetidine ring, a pyrrolidine ring, a piperidinering, or an azepane ring;

R¹ is selected from H, halo, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, and 5-6 memberedheteroaryl; wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl,phenyl, 4-7 membered heterocycloalkyl, and 5-6 membered heteroaryl areeach optionally substituted by 1, 2, or 3 independently selected R^(1A)substituents;

R^(1x) is selected from H, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl,phenyl, 4-7 membered heterocycloalkyl, and 5-6 membered heteroaryl;wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, and 5-6 membered heteroaryl are eachoptionally substituted by 1, 2, or 3 independently selected R^(1A)substituents.

R² is selected from H, halo, CN, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, and 5-6 memberedheteroaryl; wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl,phenyl, 4-7 membered heterocycloalkyl, and 5-6 membered heteroaryl areeach optionally substituted by 1, 2, or 3 independently selected R^(2A)substituents;

R^(2x) is selected from H, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl,phenyl, 4-7 membered heterocycloalkyl, and 5-6 membered heteroaryl,wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, and 5-6 membered heteroaryl are eachoptionally substituted by 1, 2, or 3 independently selected R^(2A)substituents;

R³ is selected from H;

R⁴, R^(4z), R⁵ and R^(5z) are independently selected from H; or

R⁴ and R⁵, together with the carbon atom to which they are attached,form a cyclopropyl ring;

one R⁸ is S(O)₂R^(b8); and each remaining R⁸ is independently selectedfrom H, halo, CN, C₁₋₆ alkyl, C₁₋₆ haloalkyl, OR^(a8), andNR^(c8)R^(d8); wherein said C₁₋₆ alkyl and C₁₋₆ haloalkyl are eachoptionally substituted by 1, 2, 3, or 4 independently selected R^(8A)substituents;

each R^(1A) is independently selected from halo, CN, C₁₋₆ alkyl, C₁₋₆haloalkyl, C₃₋₇ cycloalkyl, OR^(a11), and NR^(c11)R^(d11);

-   -   each R^(a11), R^(c11), and R^(d11) is independently selected        from H, C₁₋₆ alkyl and C₁₋₆ haloalkyl;

each R^(2A) is independently selected from halo, CN, NO₂, C₁₋₆ alkyl,C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, OR^(a21),and NR^(c21)R^(d21);

-   -   each R^(a21), R^(c21), and R^(d21) is independently selected        from H, C₁₋₆ alkyl and C₁₋₆ haloalkyl;

each R^(a8), R^(c8), and R^(d8) is independently selected from H, C₁₋₆alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl, wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(8A) substituents;

each R^(b8) is independently selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl,which are each optionally substituted with 1, 2, 3, or 4 independentlyselected R^(8A) substituents;

each R^(8A) is independently selected from halo, CN, NO₂, C₁₋₆ alkyl,C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₁₋₆ haloalkyl, C₃₋₄ cycloalkyl, OR^(a81),and NR^(c81)R^(d81); and

each R^(a81), R^(c81), and R^(d81) is independently selected from H,C₁₋₆ alkyl and C₁₋₆ haloalkyl.

In some embodiments:

n is 1 or 2;

Z¹ is CR⁴R⁵; and Z² is O; or

Z¹ is CR⁴R⁵; and Z² is CR^(4z)R^(5z);

R⁴ and R⁵ are each independently selected from H and C₁₋₃ alkyl;

or R⁴ and R⁵, together with the carbon atom to which they are attached,form a cyclopropyl or cyclobutyl ring;

R^(4z) and R^(5z) are each H;

Y is N;

X¹ is NR^(1x) or CR¹;

X² is NR^(2x) or CR²;

X³ is C;

wherein Ring

is an aromatic ring selected from:

Ring moiety A is azetinidin-3-yl, pyrrolidin-3-yl, piperidin-3-yl, orpiperidin-4-yl;

R¹ is selected from H, halo, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl;wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄alkyl, phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and5-6 membered heteroaryl-C₁₋₄ alkyl are each optionally substituted by 1,2, or 3 independently selected R^(1A) substituents;

R^(1x) is selected from H, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl,phenyl, 4-7 membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl;wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄alkyl, phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and5-6 membered heteroaryl-C₁₋₄ alkyl are each optionally substituted by 1,2, or 3 independently selected R^(1A) substituents;

each R^(1A) is independently selected from halo, CN, C₁₋₆ alkyl, C₁₋₆haloalkyl, C₃₋₄ cycloalkyl, OR^(a11), and NR^(c11)R^(d11);

each R^(a11), R^(c11), and R^(d11) is independently selected from H andC₁₋₆ alkyl;

R² is selected from H, halo, CN, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl;wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄alkyl, phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and5-6 membered heteroaryl-C₁₋₄ alkyl are each optionally substituted by 1,2, or 3 independently selected R^(2A) substituents;

R^(2x) is selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl,phenyl, 4-7 membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl;wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄alkyl, phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and5-6 membered heteroaryl-C₁₋₄ alkyl are each optionally substituted by 1,2, or 3 independently selected R^(2A) substituents;

each R^(2A) is independently selected from halo, CN, C₁₋₆ alkyl, C₁₋₆haloalkyl, C₃₋₄ cycloalkyl, OR^(a21), and NR^(c21)R^(d21);

each R^(a21), R^(c21), and R^(d21) is independently selected from H andC₁₋₆ alkyl;

one R⁸ is S(O)₂R^(b8);

the remaining R⁸ is selected from halo or C₁₋₃ alkyl;

each R^(b8) is independently selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl,which are each optionally substituted with 1, 2, or 3 independentlyselected R^(8A) substituents;

each R^(8A) is independently selected from halo, CN, NO₂, C₁₋₆ alkyl,C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl,5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl,4-7 membered heterocycloalkyl-C₁₋₄ alkyl, 5-6 membered heteroaryl-C₁₋₄alkyl, OR^(a81), SR^(a81), C(O)R^(b81), C(O)NR^(c81)R^(d81),C(O)OR^(a81), OC(O)R^(a81), OC(O)NR^(c81)R^(d81), NR^(c81)R^(d81),NR^(c81)C(O)R^(b81), NR^(c11)C(O)OR^(a81), NR^(c81)C(O)NR^(c81)R^(d81),NR^(c81)S(O)₂R^(b81), NR^(c81)S(O)₂NR^(c81)R^(d81), S(O)₂R^(b81), andS(O)₂NR^(c81)R^(d81), wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(8B) substituents;

each R^(a81), R^(c81), and R^(d81) is independently selected from H,C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl, wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(8B) substituents;

each R^(b81) is independently selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl,which are each optionally substituted with 1, 2, 3, or 4 independentlyselected R^(8B) substituents;

each R^(8B) is independently selected from halo, CN, C₁₋₆ alkyl, C₁₋₆haloalkyl, C₃₋₄ cycloalkyl, OR^(a82), SR^(a82), C(O)R^(b82),C(O)NR^(c82)R^(b82), C(O)OR^(a82), OC(O)R^(b82), OC(O)NR^(c82)R^(d82),NR^(c82)R^(d82), NR^(c82)C(O)R^(b82), NR^(c82)C(O)OR^(a82),NR^(c82)C(O)NR^(c82)R^(d82), NR^(c82)S(O)₂R^(b82),NR^(c82)S(O)₂NR^(c82)R^(d82), S(O)₂R^(b82), and S(O)₂NR^(c82)R^(d82);

each R^(a82), R^(c82), and R^(d82) is independently selected from H,C₁₋₆ alkyl, and C₁₋₆ haloalkyl; and

each R^(b82) is independently selected from C₁₋₆ alkyl and C₁₋₆haloalkyl.

In some embodiments:

n is 1 or 2;

Z¹ is CR⁴R⁵; and Z² is O; or

Z¹ is CR⁴R⁵; and Z² is CH₂;

R⁴ and R⁵ are each independently selected from H and methyl;

R^(4z) and R^(5z) are each H;

Y is N;

X¹ is NR^(1x) or CR¹;

X² is NR^(2x) or CR²;

X³ is C;

wherein Ring

is an aromatic ring selected from:

wherein k is n−1;

R¹ is selected from H, halo, C₁₋₆ alkyl, phenyl, and 5-6 memberedheteroaryl, wherein said C₁₋₆ alkyl, phenyl and 5-6 membered heteroarylare each optionally substituted by 1 or 2 independently selected R^(1A)substituents;

R^(1x) is selected from H, C₁₋₆ alkyl, C₃₋₇ cycloalkyl, phenyl, and 4-7membered heterocycloalkyl, wherein said C₁₋₆ alkyl, C₃₋₇ cycloalkyl,phenyl, and 4-7 membered heterocycloalkyl are each optionallysubstituted by 1 or 2 independently selected R^(1A) substituents;

each R^(1A) is independently selected from halo, CN, C₁₋₆ alkyl, C₁₋₆haloalkyl, C₃₋₄ cycloalkyl, OR^(a11), and NR^(c11)R^(d11);

each R^(a11), R^(c11), and R^(d11) is independently selected from H andC₁₋₆ alkyl;

R² is selected from H, halo, CN, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, and 5-6 memberedheteroaryl;

R^(2x) is selected from H, C₁₋₆ alkyl, C₃₋₇ cycloalkyl, and phenyl-C₁₋₃alkyl;

R⁸ is C₁₋₃ alkyl;

each R^(b8) is independently selected from C₁₋₆ alkyl, C₃₋₇ cycloalkyl,phenyl, 4-7 membered heterocycloalkyl, and 5-6 membered heteroaryl,which are each optionally substituted with 1 or 2 independently selectedR^(8A) substituents;

each R^(8A) is independently selected from halo, CN, C₁₋₆ alkyl, C₁₋₆haloalkyl, and 4-7 membered heterocycloalkyl; wherein said C₁₋₆ alkyl,C₁₋₆ haloalkyl, and 4-7 membered heterocycloalkyl are each optionallysubstituted by 1 or 2 independently selected R^(8B) substituents;

each R^(8B) is selected from C₁₋₆ alkyl and OR^(a82); and

each R^(a82) is independently selected from Hand C₁₋₆ alkyl.

In some embodiments, the compound is a compound of Formula (IIa):

or a pharmaceutically acceptable salt thereof.

In some embodiments, the compound is a compound of Formula (IIb):

or a pharmaceutically acceptable salt thereof.

In some embodiments, the compound is a compound of Formula (IIc):

or a pharmaceutically acceptable salt thereof.

In some embodiments, the compound is a compound of Formula (IId):

or a pharmaceutically acceptable salt thereof.

In some embodiments, the compound is a compound of Formula (IIIa):

or a pharmaceutically acceptable salt thereof, wherein k is n−1.

In some embodiments, the compound is a compound of Formula (IIIb):

or a pharmaceutically acceptable salt thereof, wherein k is n−1.

In some embodiments, the compound is a compound of Formula (IIIc):

or a pharmaceutically acceptable salt thereof, wherein k is n−1.

In some embodiments, the compound is a compound of Formula (IIId):

or a pharmaceutically acceptable salt thereof, wherein k is n−1.

In some embodiments, the compound is a compound of Formula (IVa):

or a pharmaceutically acceptable salt thereof, wherein k is n−1.

In some embodiments, the compound is a compound of Formula (IVb):

or a pharmaceutically acceptable salt thereof, wherein k is n−1.

In some embodiments, the compound is a compound of Formula (IVc):

or a pharmaceutically acceptable salt thereof, wherein k is n−1.

In some embodiments, the compound is a compound of Formula (IVd):

or a pharmaceutically acceptable salt thereof, wherein k is n−1.

In some embodiments, 1, 2, 3, 4, 5, 6, 7, or 8 hydrogen atoms, attachedto carbon atoms of “alkyl”, “alkenyl”, “alkynyl”, “aryl”, “phenyl”,“cycloalkyl”, “heterocycloalkyl”, or “heteroaryl” substituents or “—C₁₋₄alkyl-” and “alkylene” linking groups, as described herein, areoptionally replaced by deuterium atoms.

It is further appreciated that certain features of the invention, whichare, for clarity, described in the context of separate embodiments, canalso be provided in combination in a single embodiment. Conversely,various features of the invention which are, for brevity, described inthe context of a single embodiment, can also be provided separately orin any suitable subcombination.

When an embodiment that recites “one R⁸ is S(O)₂R^(b8); and eachremaining R⁸ is independently selected from” is combined throughmultiple dependencies with a formula showing a floating —S(O)₂Rsubstituent (e.g., Formula (IIIa)), then the floating —S(O)₂RVsubstituent on the formula replaces the “one R⁸ is S(O)₂R^(b8)” phrase.In the case of such an embodiment combined with Formula (IIIa), one ofthe R⁸ substituents (of n possible R⁸ substituents) is replaced by theS(O)₂R substituent on Formula (IIIa), wherein each of the remaining R⁸substituents (there being k remaining R⁸ substituents) is independentlyselected from the “each remaining R⁸” list.

wherein k is n−1.

At various places in the present specification, divalent linkingsubstituents are described. Unless otherwise specified, it isspecifically intended that each divalent linking substituent includeboth the forward and backward forms of the linking substituent. Forexample, —NR(CR′R″)_(n)— includes both —NR(CR′R″)_(n)— and—(CR′R″)_(n)NR—. Where the structure clearly requires a linking group,the Markush variables listed for that group are understood to be linkinggroups.

The term “n-membered” where n is an integer typically describes thenumber of ring-forming atoms in a moiety where the number ofring-forming atoms is n. For example, piperidinyl is an example of a6-membered heterocycloalkyl ring, pyrazolyl is an example of a5-membered heteroaryl ring, pyridyl is an example of a 6-memberedheteroaryl ring, and 1,2,3,4-tetrahydro-naphthalene is an example of a10-membered cycloalkyl group.

As used herein, the phrase “optionally substituted” means unsubstitutedor substituted. The substituents are independently selected, andsubstitution may be at any chemically accessible position. As usedherein, the term “substituted” means that a hydrogen atom is removed andreplaced by a substituent. A single divalent substituent, e.g., oxo, canreplace two hydrogen atoms. It is to be understood that substitution ata given atom is limited by valency, that the designated atom's normalvalency is not exceeded, and that the substitution results in a stablecompound.

As used herein, the term “independently selected from” means that eachoccurrence of a variable or substituent are independently selected ateach occurrence from the applicable list.

When any variable (e.g., R^(G)) occurs more than one time in anyconstituent or formula for a compound, its definition at each occurrenceis independent of its definition at every other occurrence. Thus, forexample, if a group is shown to be substituted with 1, 2, 3, or 4 R^(G),then said group may optionally be substituted with up to four R^(G)groups and R^(G) at each occurrence is selected independently from thedefinition of R^(G). Also, combinations of substituents and/or variablesare permissible only if such combinations result in stable compounds.

In some embodiments, when an optionally multiple substituent isdesignated in the form:

then it is to be understood that substituent R can occur p number oftimes on the ring, and R can be a different moiety at each occurrence.It is to be understood that each R group may replace any hydrogen atomattached to a ring atom, including one or both of the (CH₂)_(n) hydrogenatoms. Further, in the above example, should the variable Q be definedto include hydrogens, such as when Q is said to be CH₂, NH, etc., anyfloating substituent such as R in the above example, can replace ahydrogen of the Q variable as well as a hydrogen in any othernon-variable component of the ring.

Throughout the definitions, the term “C_(n-m)” indicates a range whichincludes the endpoints, wherein n and m are integers and indicate thenumber of carbons. Examples include C₁₋₃, C₁₋₄, C₁₋₆, and the like.

As used herein, the term “C_(n-m) alkyl”, employed alone or incombination with other terms, refers to a saturated hydrocarbon groupthat may be straight-chain or branched, having n to m carbons. Examplesof alkyl moieties include, but are not limited to, chemical groups suchas methyl (Me), ethyl (Et), n-propyl (n-Pr), isopropyl (i-Pr), n-butyl,tert-butyl, isobutyl, sec-butyl; higher homologs such as2-methyl-1-butyl, n-pentyl, 3-pentyl, n-hexyl, 1,2,2-trimethylpropyl,and the like. In some embodiments, the alkyl group contains from 1 to 6carbon atoms, from 1 to 4 carbon atoms, from 1 to 3 carbon atoms, or 1to 2 carbon atoms.

As used herein, “C_(n-m) alkenyl” refers to an alkyl group having one ormore double carbon-carbon bonds and having n to m carbons. Examplealkenyl groups include, but are not limited to, ethenyl, n-propenyl,isopropenyl, n-butenyl, sec-butenyl, and the like. In some embodiments,the alkenyl moiety contains 2 to 6, 2 to 4, or 2 to 3 carbon atoms.

As used herein, “C_(n-m) alkynyl” refers to an alkyl group having one ormore triple carbon-carbon bonds and having n to m carbons. Examplealkynyl groups include, but are not limited to, ethynyl, propyn-1-yl,propyn-2-yl, and the like. In some embodiments, the alkynyl moietycontains 2 to 6, 2 to 4, or 2 to 3 carbon atoms. As used herein, theterm “C_(n-m) alkoxy”, employed alone or in combination with otherterms, refers to a group of formula-O-alkyl, wherein the alkyl group hasn to m carbons. Example alkoxy groups include, but are not limited to,methoxy, ethoxy, propoxy (e.g., n-propoxy and isopropoxy), butoxy (e.g.,n-butoxy and tert-butoxy), and the like. In some embodiments, the alkylgroup has 1 to 6, 1 to 4, or 1 to 3 carbon atoms.

As used herein, the term “amino” refers to a group of formula —NH₂.

As used herein, the term “aryl,” employed alone or in combination withother terms, refers to an aromatic hydrocarbon group, which may bemonocyclic or polycyclic (e.g., having 2 or 3 fused rings). The term“C_(n-m) aryl” refers to an aryl group having from n to m ring carbonatoms. Aryl groups include, e.g., phenyl, naphthyl, anthracenyl,phenanthrenyl, indanyl, indenyl, and the like. In some embodiments, thearyl group has from 6 to 10 carbon atoms. In some embodiments, the arylgroup is phenyl or naphthyl. In some embodiments, the aryl is phenyl.

As used herein, “halo” refers to F, Cl, Br, or I. In some embodiments,halo is F, Cl, or Br. In some embodiments, halo is F or Cl. In someembodiments, halo is F. In some embodiments, halo is Cl.

As used herein, “C_(n-m) haloalkoxy” refers to a group of formula—O-haloalkyl having n to m carbon atoms. Example haloalkoxy groupsinclude OCF₃ and OCHF₂. In some embodiments, the haloalkoxy group isfluorinated only. In some embodiments, the alkyl group has 1 to 6, 1 to4, or 1 to 3 carbon atoms.

As used herein, the term “C_(n-m) haloalkyl”, employed alone or incombination with other terms, refers to an alkyl group having from onehalogen atom to 2s+1 halogen atoms which may be the same or different,where “s” is the number of carbon atoms in the alkyl group, wherein thealkyl group has n to m carbon atoms. In some embodiments, the haloalkylgroup is fluorinated only. In some embodiments, the alkyl group has 1 to6, 1 to 4, or 1 to 3 carbon atoms. Example haloalkyl groups include CF₃,C₂F₅, CHF₂, CH₂F, CCl₃, CHCl₂, C₂Cl₅ and the like.

As used herein, the term “C_(n-m) fluoroalkyl” refers to an alkyl grouphaving from one fluoro atom to 2s+1 fluoro atoms, where “s” is thenumber of carbon atoms in the alkyl group, wherein the alkyl group has nto m carbon atoms. In some embodiments, the alkyl group has 1 to 6, 1 to4, or 1 to 3 carbon atoms. Example haloalkyl groups include CF₃, C₂F₅,CHF₂, CH₂F, and the like.

As used herein, the term “thio” refers to a group of formula —SH.

As used herein, the term “C_(n-m) alkylamino” refers to a group offormula —NH(alkyl), wherein the alkyl group has n to m carbon atoms. Insome embodiments, the alkyl group has 1 to 6, 1 to 4, or 1 to 3 carbonatoms.

As used herein, the term “C_(n-m) alkoxycarbonyl” refers to a group offormula —C(O)O-alkyl, wherein the alkyl group has n to m carbon atoms.In some embodiments, the alkyl group has 1 to 6, 1 to 4, or 1 to 3carbon atoms.

As used herein, the term “C_(n-m) alkylcarbonyl” refers to a group offormula —C(O)— alkyl, wherein the alkyl group has n to m carbon atoms.In some embodiments, the alkyl group has 1 to 6, 1 to 4, or 1 to 3carbon atoms.

As used herein, the term “C_(n-m) alkylcarbonylamino” refers to a groupof formula —NHC(O)-alkyl, wherein the alkyl group has n to m carbonatoms. In some embodiments, the alkyl group has 1 to 6, 1 to 4, or 1 to3 carbon atoms.

As used herein, the term “C_(n-m) alkoxycarbonylamino” refers to a groupof formula —NHC(O)O(C_(n-m) alkyl), wherein the alkyl group has n to mcarbon atoms. In some embodiments, the alkyl group has 1 to 6, 1 to 4,or 1 to 3 carbon atoms.

As used herein, the term “C_(n-m) alkylsulfonylamino” refers to a groupof formula —NHS(O)₂-alkyl, wherein the alkyl group has n to m carbonatoms. In some embodiments, the alkyl group has 1 to 6, 1 to 4, or 1 to3 carbon atoms.

As used herein, the term “aminosulfonyl” refers to a group of formula—S(O)₂NH₂.

As used herein, the term “C_(n-m) alkylaminosulfonyl” refers to a groupof formula —S(O)₂NH(alkyl), wherein the alkyl group has n to m carbonatoms. In some embodiments, the alkyl group has 1 to 6, 1 to 4, or 1 to3 carbon atoms.

As used herein, the term “di(C_(n-m) alkyl)aminosulfonyl” refers to agroup of formula —S(O)₂N(alkyl)₂, wherein each alkyl group independentlyhas n to m carbon atoms. In some embodiments, each alkyl group has,independently, 1 to 6, 1 to 4, or 1 to 3 carbon atoms.

As used herein, the term “aminosulfonylamino” refers to a group offormula —NHS(O)₂NH₂.

As used herein, the term “C_(n-m) alkylaminosulfonylamino” refers to agroup of formula —NHS(O)₂NH(alkyl), wherein the alkyl group has n to mcarbon atoms. In some embodiments, the alkyl group has 1 to 6, 1 to 4,or 1 to 3 carbon atoms.

As used herein, the term “di(C_(n-m) alkyl)aminosulfonylamino” refers toa group of formula —NHS(O)₂N(alkyl)₂, wherein each alkyl groupindependently has n to m carbon atoms. In some embodiments, each alkylgroup has, independently, 1 to 6, 1 to 4, or 1 to 3 carbon atoms.

As used herein, the term “aminocarbonylamino”, employed alone or incombination with other terms, refers to a group of formula —NHC(O)NH₂.

As used herein, the term “C_(n-m) alkylaminocarbonylamino” refers to agroup of formula —NHC(O)NH(alkyl), wherein the alkyl group has n to mcarbon atoms. In some embodiments, the alkyl group has 1 to 6, 1 to 4,or 1 to 3 carbon atoms.

As used herein, the term “di(C_(n-m) alkyl)aminocarbonylamino” refers toa group of formula —NHC(O)N(alkyl)₂, wherein each alkyl groupindependently has n to m carbon atoms. In some embodiments, each alkylgroup has, independently, 1 to 6, 1 to 4, or 1 to 3 carbon atoms.

As used herein, the term “C_(n-m) alkylcarbamoyl” refers to a group offormula —C(O)—NH(alkyl), wherein the alkyl group has n to m carbonatoms. In some embodiments, the alkyl group has 1 to 6, 1 to 4, or 1 to3 carbon atoms.

As used herein, the term “C_(n-m) alkylthio” refers to a group offormula —S-alkyl, wherein the alkyl group has n to m carbon atoms. Insome embodiments, the alkyl group has 1 to 6, 1 to 4, or 1 to 3 carbonatoms.

As used herein, the term “C_(n-m) alkylsulfinyl” refers to a group offormula —S(O)— alkyl, wherein the alkyl group has n to m carbon atoms.In some embodiments, the alkyl group has 1 to 6, 1 to 4, or 1 to 3carbon atoms.

As used herein, the term “C_(n-m) alkylsulfonyl” refers to a group offormula —S(O)₂-alkyl, wherein the alkyl group has n to m carbon atoms.In some embodiments, the alkyl group has 1 to 6, 1 to 4, or 1 to 3carbon atoms.

As used herein, the term “cyano-C_(n-m) alkyl” refers to a group offormula —(C_(n-m) alkylene)-CN, wherein the alkylene group has n to mcarbon atoms. As used herein, the term “cyano-C₁₋₃ alkyl” refers to agroup of formula —(C₁₋₃ alkylene)-CN.

As used herein, the term “HO—C_(n-m) alkyl” refers to a group of formulaC_(n-m) alkylene)-OH, wherein the alkylene group has n to m carbonatoms. As used herein, the term “HO—C₁₋₃ alkyl” refers to a group offormula —(C₁₋₃ alkylene)-OH.

As used herein, the term “C_(n-m) alkoxy-C_(o-p) alkyl” refers to agroup of formula —(C_(n-m) alkylene)-O(C_(o-p) alkyl), wherein thealkylene group has n to m carbon atoms and the alkyl group has o to pcarbon atoms. As used herein, the term “C₁₋₃ alkoxy-C₁₋₃ alkyl” refersto a group of formula —(C₁₋₃ alkylene)-O(C₁₋₃ alkyl).

As used herein, the term “carboxy” refers to a group of formula —C(O)OH.

As used herein, the term “di(C_(n-m)-alkyl)amino” refers to a group offormula —N(alkyl)₂, wherein the two alkyl groups each has,independently, n to m carbon atoms. In some embodiments, each alkylgroup independently has 1 to 6, 1 to 4, or 1 to 3 carbon atoms.

As used herein, the term “di(C_(n-m)-alkyl)carbamyl” refers to a groupof formula —C(O)N(alkyl)₂, wherein the two alkyl groups each has,independently, n to m carbon atoms. In some embodiments, each alkylgroup independently has 1 to 6, 1 to 4, or 1 to 3 carbon atoms.

As used herein, the term “C_(n-m) alkylcarbonyloxy” is a group offormula —OC(O)— alkyl, wherein the alkyl group has n to m carbon atoms.In some embodiments, the alkyl group has 1 to 6, 1 to 4, or 1 to 3carbon atoms.

As used herein, “aminocarbonyloxy” is a group of formula —OC(O)—NH₂.

As used herein, “C_(n-m) alkylaminocarbonyloxy” is a group of formula—OC(O)—NH— alkyl, wherein the alkyl group has n to m carbon atoms. Insome embodiments, the alkyl group has 1 to 6, 1 to 4, or 1 to 3 carbonatoms.

As used herein, “di(C_(n-m)alkyl)aminocarbonyloxy” is a group of formula—OC(O)—N(alkyl)₂, wherein each alkyl group has, independently, n to mcarbon atoms. In some embodiments, each alkyl group independently has 1to 6, 1 to 4, or 1 to 3 carbon atoms.

As used herein C_(n-m) alkoxycarbonylamino refers to a group of formula—NHC(O)—O-alkyl, wherein the alkyl group has n to m carbon atoms.

As used herein, the term “carbamyl” to a group of formula —C(O)NH₂.

As used herein, the term “carbonyl”, employed alone or in combinationwith other terms, refers to a —C(O)— group.

As used herein, “cycloalkyl” refers to non-aromatic cyclic hydrocarbonsincluding cyclized alkyl and alkenyl groups. Cycloalkyl groups caninclude mono- or polycyclic (e.g., having 2, 3 or 4 fused rings) groups,spirocycles, and bridged rings (e.g., a bridged bicycloalkyl group).Ring-forming carbon atoms of a cycloalkyl group can be optionallysubstituted by oxo or sulfido (e.g., C(O) or C(S)). Also included in thedefinition of cycloalkyl are moieties that have one or more aromaticrings fused (i.e., having a bond in common with) to the cycloalkyl ring,for example, benzo or thienyl derivatives of cyclopentane, cyclohexane,and the like. A cycloalkyl group containing a fused aromatic ring can beattached through any ring-forming atom including a ring-forming atom ofthe fused aromatic ring. Cycloalkyl groups can have 3, 4, 5, 6, 7, 8, 9,10, 11, 12, 13 or 14 ring-forming carbons (i.e., C₃₋₁₄). In someembodiments, cycloalkyl is C₃₋₁₄ cycloalkyl, wherein 1, 2, 3, or 4ring-forming carbon atoms of said C₃₋₁₄ cycloalkyl can be optionallysubstituted by one or more oxo or sulfido. In some embodiments, thecycloalkyl is a C₃₋₁₀ monocyclic or bicyclic cycloalkyl. In someembodiments, the cycloalkyl is a C₃₋₇ monocyclic cycloalkyl. In someembodiments, the cycloalkyl is a C₄₋₇ monocyclic cycloalkyl. In someembodiments, the cycloalkyl is a C₄₋₁₄ spirocycle or bridged cycloalkyl(e.g., a bridged bicycloalkyl group). Example cycloalkyl groups includecyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl,cyclopentenyl, cyclohexenyl, cyclohexadienyl, cycloheptatrienyl,norbornyl, norpinyl, norcarnyl, cubane, adamantane,bicyclo[1.1.1]pentyl, bicyclo[2.1.1]hexyl, bicyclo[2.2.1]heptanyl,bicyclo[3.1.1]heptanyl, bicyclo[2.2.2]octanyl, spiro[3.3]heptanyl, andthe like. In some embodiments, cycloalkyl is cyclopropyl, cyclobutyl,cyclopentyl, or cyclohexyl.

As used herein, “heteroaryl” refers to a monocyclic or polycyclic (e.g.,having 2, 3, or 4 fused rings) aromatic heterocycle having at least oneheteroatom ring member selected from N, O, S and B. In some embodiments,the heteroaryl ring has 1, 2, 3, or 4 heteroatom ring membersindependently selected from N, O, S and B. In some embodiments, anyring-forming N in a heteroaryl moiety can be an N-oxide. In someembodiments, the heteroaryl is a monocyclic, bicyclic, or tricyclic 5-14membered heteroaryl having 1, 2, 3, or 4 heteroatom ring membersindependently selected from N, O, and S. In some embodiments, theheteroaryl is a 5-10 membered monocyclic or bicyclic heteroaryl having1, 2, 3, or 4 heteroatom ring members independently selected from N, O,and S. In some embodiments, the heteroaryl is a 5-6 monocyclicheteroaryl having 1 or 2 heteroatom ring members independently selectedfrom N, O, and S. In some embodiments, the heteroaryl is a 5-6monocyclic heteroaryl having 1 or 2 heteroatom ring membersindependently selected from N, O, and S. In some embodiments, theheteroaryl group contains 5 to 14, 5 to 10, 5 to 6, or 5 to 6ring-forming atoms. In some embodiments, the heteroaryl group has 1 to 4ring-forming heteroatoms, 1 to 3 ring-forming heteroatoms, 1 to 2ring-forming heteroatoms or 1 ring-forming heteroatom. When theheteroaryl group contains more than one heteroatom ring member, theheteroatoms may be the same or different. Example heteroaryl groupsinclude, but are not limited to, pyridyl, pyrimidinyl, pyrazinyl,pyridazinyl, pyrrolyl, pyrazolyl, azolyl, oxazolyl, isoxazolyl,thiazolyl, isothiazolyl, imidazolyl, furyl, thienyl, triazolyl (e.g.,1,2,3-triazolyl, 1,2,4-triazolyl, 1,3,4-triazolyl), tetrazolyl,thiadiazolyl (e.g., 1,2,3-thiadiazolyl, 1,2,4-thiadiazolyl,1,3,4-thiadiazolyl), quinolinyl, isoquinolinyl, indolyl, benzothienyl,benzofuran, benzisoxazole, imidazo[1, 2-b]thiazolyl, purinyl, triazinyl,thieno[3,2-b]pyridinyl, imidazo[1,2-a]pyridinyl, 1,5-naphthyridinyl,1H-pyrazolo[4,3-b]pyridinyl, and oxadiazolyl (e.g., 1,2,3-oxadiazolyl,1,2,4-oxadiazolyl, 1,3,4-oxadiazolyl).

As used herein, “heterocycloalkyl” refers to monocyclic or polycyclicheterocycles having at least one non-aromatic ring (saturated orpartially unsaturated ring), wherein one or more of the ring-formingcarbon atoms of the heterocycloalkyl is replaced by a heteroatomselected from N, O, S, and B, and wherein the ring-forming carbon atomsand heteroatoms of the heterocycloalkyl group can be optionallysubstituted by one or more oxo or sulfido (e.g., C(O), S(O), C(S), orS(O)₂, etc.). Heterocycloalkyl groups include monocyclic and polycyclic(e.g., having 2 fused rings) systems. Included in heterocycloalkyl aremonocyclic and polycyclic 4-14, 4-12, 4-10, 5-7, 4-7, 4-6, and5-6-membered heterocycloalkyl groups. Heterocycloalkyl groups can alsoinclude spirocycles and bridged rings (e.g., a 5-10 membered bridgedbiheterocycloalkyl ring having one or more of the ring-forming carbonatoms replaced by a heteroatom independently selected from N, O, S, andB). The heterocycloalkyl group can be attached through a ring-formingcarbon atom or a ring-forming heteroatom. In some embodiments, theheterocycloalkyl group contains 0 to 3 double bonds. In someembodiments, the heterocycloalkyl group contains 0 to 2 double bonds.

Also included in the definition of heterocycloalkyl are moieties thathave one or more aromatic rings fused (i.e., having a bond in commonwith) to the non-aromatic heterocyclic ring, for example, benzo orthienyl derivatives of piperidine, morpholine, azepine, etc. Aheterocycloalkyl group containing a fused aromatic ring can be attachedthrough any ring-forming atom including a ring-forming atom of the fusedaromatic ring. In some embodiments, the heterocycloalkyl group contains4 to 14 ring-forming atoms, 4 to 10 ring-forming atoms, 4 to 7ring-forming atoms, 4 to 6 ring-forming atoms, or 5 to 6 ring-formingatoms. In some embodiments, the heterocycloalkyl group has 1 to 4heteroatoms, 1 to 3 heteroatoms, 1 to 2 heteroatoms or 1 heteroatom.

In some embodiments, the heterocycloalkyl is a 4-14 membered monocyclic,bicyclic, or tricyclic heterocycloalkyl having 1, 2, 3, or 4ring-forming heteroatoms independently selected from N, O, and S,wherein 1, 2, 3, or 4 ring-forming carbon or heteroatoms can beoptionally substituted by one or more oxo or sulfido. In someembodiments, the heterocycloalkyl is a 4-10 membered monocyclic,bicyclic, or tricyclic heterocycloalkyl having 1, 2, 3, or 4ring-forming heteroatoms independently selected from N, O, and S,wherein 1, 2, 3, or 4 ring-forming carbon or heteroatoms can beoptionally substituted by one or more oxo or sulfido. In someembodiments, the heterocycloalkyl is a 4-7 membered monocyclicheterocycloalkyl having 1 or 2 ring-forming heteroatoms independentlyselected from N, O, and S, and wherein 1, 2 or 3 ring-forming carbon orheteroatoms can be optionally substituted by one or more oxo or sulfido.In some embodiments, the heterocycloalkyl is a monocyclic 4-6 memberedheterocycloalkyl having 1 or 2 heteroatoms independently selected fromN, O, and S and having one or more oxidized ring members. Exampleheterocycloalkyl groups include pyrrolidin-2-one,1,3-isoxazolidin-2-one, pyranyl, tetrahydropyran, oxetanyl, azetidinyl,morpholino, thiomorpholino, piperazinyl, tetrahydrofuranyl,tetrahydrothienyl, piperidinyl, pyrrolidinyl, isoxazolidinyl,isothiazolidinyl, pyrazolidinyl, oxazolidinyl, thiazolidinyl,imidazolidinyl, azepanyl, benzazapene, 1,2,3,4-tetrahydroisoquinoline,azabicyclo[3.1.0]hexanyl, diazabicyclo[3.1.0]hexanyl,oxabicyclo[2.1.1]hexanyl, azabicyclo[2.2.1]heptanyl,diazabicyclo[2.2.1]heptanyl, azabicyclo[3.1.1]heptanyl,diazabicyclo[3.1.1]heptanyl, azabicyclo[3.2.1]octanyl,diazabicyclo[3.2.1]octanyl, oxabicyclo[2.2.2]octanyl,azabicyclo[2.2.2]octanyl, azaadamantanyl, diazaadamantanyl,oxa-adamantanyl, azaspiro[3.3]heptanyl, diazaspiro[3.3]heptanyl,oxa-azaspiro[3.3]heptanyl, azaspiro[3.4]octanyl, diazaspiro[3.4]octanyl,oxa-azaspiro[3.4]octanyl, azaspiro[2.5]octanyl, diazaspiro[2.5]octanyl,azaspiro[4.4]nonanyl, diazaspiro[4.4]nonanyl, oxa-azaspiro[4.4]nonanyl,azaspiro[4.5]decanyl, diazaspiro[4.5]decanyl, diazaspiro[4.4]nonanyl,oxa-diazaspiro[4.4]nonanyl, and the like.

As used herein, “C_(o-p) cycloalkyl-C_(n-m) alkyl-” refers to a group offormula cycloalkyl-alkylene-, wherein the cycloalkyl has o to p carbonring members and the alkylene linking group has n to m carbon atoms.

As used herein “C_(o-p) aryl-C_(n-m) alkyl-” refers to a group offormula aryl-alkylene-, wherein the aryl has o to p carbon ring membersand the alkylene linking group has n to m carbon atoms.

As used herein, “heteroaryl-C_(n-m) alkyl-” refers to a group of formulaheteroaryl-alkylene-, wherein alkylene linking group has n to m carbonatoms.

As used herein “heterocycloalkyl-C_(n-m) alkyl-” refers to a group offormula heterocycloalkyl-alkylene-, wherein alkylene linking group has nto m carbon atoms.

As used herein, an “alkyl linking group” is a bivalent straight chain orbranched alkyl linking group (“alkylene group”). For example, “C_(o-p)cycloalkyl-C_(n-m) alkyl-”, “C_(o-p) aryl-C_(n-m) alkyl-”,“phenyl-C_(n-m) alkyl-”, “heteroaryl-C_(n-m) alkyl-”, and“heterocycloalkyl-C_(n-m) alkyl-” contain alkyl linking groups. Examplesof “alkyl linking groups” or “alkylene groups” include methylene,ethan-1,1-diyl, ethan-1,2-diyl, propan-1,3-dilyl, propan-1,2-diyl,propan-1,1-diyl and the like.

As used herein, the term “oxo” refers to an oxygen atom (i.e., ═O) as adivalent substituent, forming a carbonyl group when attached to a carbon(e.g., C═O or C(O)), or attached to a nitrogen or sulfur heteroatomforming a nitroso, sulfinyl or sulfonyl group.

At certain places, the definitions or embodiments refer to specificrings (e.g., an azetidine ring, a pyridine ring, etc.). Unless otherwiseindicated, these rings can be attached to any ring member provided thatthe valency of the atom is not exceeded. For example, an azetidine ringmay be attached at any position of the ring, whereas a pyridin-3-yl ringis attached at the 3-position.

The compounds described herein can be asymmetric (e.g., having one ormore stereocenters). All stereoisomers, such as enantiomers anddiastereomers, are intended unless otherwise indicated. Compounds of thepresent disclosure that contain asymmetrically substituted carbon atomscan be isolated in optically active or racemic forms. Methods on how toprepare optically active forms from optically inactive startingmaterials are known in the art, such as by resolution of racemicmixtures or by stereoselective synthesis. Many geometric isomers ofolefins, C═N double bonds, and the like can also be present in thecompounds described herein, and all such stable isomers are contemplatedin the present invention. Cis and trans geometric isomers of thecompounds of the present disclosure are described and may be isolated asa mixture of isomers or as separated isomeric forms. In someembodiments, the compound has the (R)-configuration. In someembodiments, the compound has the (S)-configuration. The Formulas (e.g.,Formula (I), (II), etc.) provided herein include stereoisomers of thecompounds.

Resolution of racemic mixtures of compounds can be carried out by any ofnumerous methods known in the art. An example method includes fractionalrecrystallization using a chiral resolving acid which is an opticallyactive, salt-forming organic acid. Suitable resolving agents forfractional recrystallization methods are, for example, optically activeacids, such as the D and L forms of tartaric acid, diacetyltartaricacid, dibenzoyltartaric acid, mandelic acid, malic acid, lactic acid orthe various optically active camphorsulfonic acids such asβ-camphorsulfonic acid. Other resolving agents suitable for fractionalcrystallization methods include stereoisomerically pure forms ofα-methylbenzylamine (e.g., S and R forms, or diastereomerically pureforms), 2-phenylglycinol, norephedrine, ephedrine, N-methylephedrine,cyclohexylethylamine, 1,2-diaminocyclohexane, and the like.

Resolution of racemic mixtures can also be carried out by elution on acolumn packed with an optically active resolving agent (e.g.,dinitrobenzoylphenylglycine). Suitable elution solvent composition canbe determined by one skilled in the art.

Compounds provided herein also include tautomeric forms. Tautomericforms result from the swapping of a single bond with an adjacent doublebond together with the concomitant migration of a proton. Tautomericforms include prototropic tautomers which are isomeric protonationstates having the same empirical formula and total charge. Exampleprototropic tautomers include ketone-enol pairs, amide-imidic acidpairs, lactam-lactim pairs, enamine-imine pairs, and annular forms wherea proton can occupy two or more positions of a heterocyclic system, forexample, 1H- and 3H-imidazole, 1H-, 2H- and 4H-1,2,4-triazole, 1H- and2H-isoindole, 2-hydroxypyridine and 2-pyridone, and 1H- and 2H-pyrazole.Tautomeric forms can be in equilibrium or sterically locked into oneform by appropriate substitution.

All compounds, and pharmaceutically acceptable salts thereof, can befound together with other substances such as water and solvents (e.g.,hydrates and solvates) or can be isolated.

In some embodiments, preparation of compounds can involve the additionof acids or bases to affect, for example, catalysis of a desiredreaction or formation of salt forms such as acid addition salts.

In some embodiments, the compounds provided herein, or salts thereof,are substantially isolated. By “substantially isolated” is meant thatthe compound is at least partially or substantially separated from theenvironment in which it was formed or detected. Partial separation caninclude, for example, a composition enriched in the compounds providedherein. Substantial separation can include compositions containing atleast about 50%, at least about 60%, at least about 70%, at least about80%, at least about 90%, at least about 95%, at least about 97%, or atleast about 99% by weight of the compounds provided herein, or saltthereof. Methods for isolating compounds and their salts are routine inthe art.

The term “compound” as used herein is meant to include allstereoisomers, geometric isomers, tautomers, and isotopes of thestructures depicted. Compounds herein identified by name or structure asone particular tautomeric form are intended to include other tautomericforms unless otherwise specified.

The phrase “pharmaceutically acceptable” is employed herein to refer tothose compounds, materials, compositions, and/or dosage forms which are,within the scope of sound medical judgment, suitable for use in contactwith the tissues of human beings and animals without excessive toxicity,irritation, allergic response, or other problem or complication,commensurate with a reasonable benefit/risk ratio.

The present application also includes pharmaceutically acceptable saltsof the compounds described herein. As used herein, “pharmaceuticallyacceptable salts” refers to derivatives of the disclosed compoundswherein the parent compound is modified by converting an existing acidor base moiety to its salt form. Examples of pharmaceutically acceptablesalts include, but are not limited to, mineral or organic acid salts ofbasic residues such as amines; alkali or organic salts of acidicresidues such as carboxylic acids; and the like. The pharmaceuticallyacceptable salts of the present disclosure include the conventionalnon-toxic salts of the parent compound formed, for example, fromnon-toxic inorganic or organic acids. The pharmaceutically acceptablesalts of the present disclosure can be synthesized from the parentcompound which contains a basic or acidic moiety by conventionalchemical methods. Generally, such salts can be prepared by reacting thefree acid or base forms of these compounds with a stoichiometric amountof the appropriate base or acid in water or in an organic solvent, or ina mixture of the two; generally, non-aqueous media like ether, ethylacetate, alcohols (e.g., methanol, ethanol, iso-propanol, or butanol) oracetonitrile (ACN) are preferred. Lists of suitable salts are found inRemington's Pharmaceutical Sciences, 17th ed., Mack Publishing Company,Easton, Pa., 1985, p. 1418 and Journal of Pharmaceutical Science, 66, 2(1977), each of which is incorporated herein by reference in itsentirety.

Synthesis

As will be appreciated by those skilled in the art, the compoundsprovided herein, including salts and stereoisomers thereof, can beprepared using known organic synthesis techniques and can be synthesizedaccording to any of numerous possible synthetic routes, such as thoseprovided in the in the Schemes below.

The reactions for preparing compounds described herein can be carriedout in suitable solvents which can be readily selected by one of skillin the art of organic synthesis. Suitable solvents can be substantiallynon-reactive with the starting materials (reactants), the intermediatesor products at the temperatures at which the reactions are carried out,e.g., temperatures which can range from the solvent's freezingtemperature to the solvent's boiling temperature. A given reaction canbe carried out in one solvent or a mixture of more than one solvent.Depending on the particular reaction step, suitable solvents for aparticular reaction step can be selected by the skilled artisan.

The expressions, “ambient temperature” or “room temperature” or “rt” asused herein, are understood in the art, and refer generally to atemperature, e.g., a reaction temperature, that is about the temperatureof the room in which the reaction is carried out, for example, atemperature from about 20° C. to about 30° C.

Preparation of compounds described herein can involve the protection anddeprotection of various chemical groups. The need for protection anddeprotection, and the selection of appropriate protecting groups, can bereadily determined by one skilled in the art. The chemistry ofprotecting groups can be found, for example, e.g., Wuts et al.,Protective Groups in Organic Synthesis, 4th Ed., (Wiley, 2006).

Reactions can be monitored according to any suitable method known in theart. For example, product formation can be monitored by spectroscopicmeans, such as nuclear magnetic resonance spectroscopy (e.g., ¹H or¹³C), infrared spectroscopy, spectrophotometry (e.g., UV-visible), massspectrometry or by chromatographic methods such as high performanceliquid chromatography (HPLC), liquid chromatography-mass spectroscopy(LCMS), or thin layer chromatography (TLC). Compounds can be purified bythose skilled in the art by a variety of methods, including highperformance liquid chromatography (HPLC) and normal phase silicachromatography.

The Schemes below provide general guidance in connection with preparingthe compounds of the invention. One skilled in the art would understandthat the preparations shown in the Schemes can be modified or optimizedusing general knowledge of organic chemistry to prepare variouscompounds of the invention.

Compounds of formula 1-4 can be prepared by the general syntheticprocedure illustrated in Scheme 1. Appropriately substituted compoundsof formula 1-1 can react with1-tert-butoxy-N,N,N′,N′-tetramethylmethanediamine to provide compoundsof formula 1-2. Compounds of formula 1-2 can then be converted intocompounds of formula 1-4 via reaction with appropriately substitutedcompounds of formula 1-3 in the presence of a suitable base such asNaOEt.

Compounds of formula 2-7 with a variety of substitution can be preparedusing the processes illustrated in Scheme 2. As shown in Scheme 2,appropriately substituted compounds of formula 2-1 can react withappropriately substituted compounds of formula 2-2 in the presence of asuitable base such as DIPEA to provide compounds of formula 2-3.Compounds of formula 2-3 can then be converted into compounds of formula2-4 via rearrangement reaction in the presence of a suitable Lewis acidsuch as AlCl₃. Compounds of formula 2-5 can be accessed via the reactionof compounds of formula 2-4 with oxalyl chloride. Compounds of formula2-5 can react with appropriately substituted compounds of formula 2-6 inthe presence of a suitable base such as DIPEA to yield compounds offormula 2-7. Alternatively, compounds of formula 2-5 can react withappropriately substituted compounds of formula 2-8 in the presence of asuitable base such as DIPEA to provide compounds of formula 2-9 whichcan be then converted into compounds of formula 2-7 by deprotection ofBoc protecting group under acidic condition.

Compounds of formula 3-7 with a variety of substitution can be preparedusing the processes illustrated in Scheme 3. As shown in Scheme 3,appropriately substituted compounds of formula 3-1 can react with NBS toprovide compounds of formula 3-2. Compounds of formula 3-4 can beaccessed via the reaction of compounds of formula 3-2 with appropriatelysubstituted compounds of formula 3-3 and a suitable base such as K₂CO₃.Compounds of formula 3-4 can then be converted into compounds of formula3-7 by a number of methods, e.g., by nucleophilic substitution withappropriately substituted halides of formula 3-6 (X=Cl, Br or I) in asuitable solvent (e.g., DMSO, DMF, 1,4-dioxane) with or without asuitable base (e.g., triethylamine, N,N-diisopropylethylamine, orCs₂CO₃), or by a suitable C—N cross-coupling, including Chan-Lamcoupling with appropriately substituted boronic acids of formula 3-5(e.g., in the presence of a copper catalyst such as Cu(OAc)₂, a ligandsuch as 2,2′-bipyridine and a base such as K₂CO₃) in a suitable solvent(e.g., 1,4-dioxane or 1,2-dichloroethane).

Alternatively, compounds of formula 3-7 with a variety of substitutioncan also be prepared using the processes illustrated in Scheme 4. Asshown in Scheme 4, appropriately substituted compounds of formula 4-1can react with appropriately substituted compounds of formula 4-2 in thepresence of a suitable base such as Et₃N to provide compounds of formula4-3. Compounds of formula 4-3 can then be converted into compounds offormula 3-7 by reacting with appropriately substituted compounds offormula 3-2 in the presence of a suitable base such as Et₃N.

Compounds of formula 1-3 with a variety of substitution can be preparedusing the processes illustrated in Scheme 5. Appropriately substitutedcompounds of formula 5-1 can react with 1H-pyrazole-1-carboximidamidehydrochloride in the presence of a suitable base such as DIPEA toprovide compounds of formula 1-3.

METHODS OF USE

Compounds of the present disclosure can inhibit CDK2 and therefore areuseful for treating diseases wherein the underlying pathology is, whollyor partially, mediated by CDK2. Such diseases include cancer and otherdiseases with proliferation disorder. In some embodiments, the presentdisclosure provides treatment of an individual or a patient in vivousing a compound of Formula (I) or a salt or stereoisomer thereof suchthat growth of cancerous tumors is inhibited. A compound of Formula (I)or of any of the formulas as described herein, or a compound as recitedin any of the claims and described herein, or a salt or stereoisomerthereof, can be used to inhibit the growth of cancerous tumors withaberrations that activate the CDK2 kinase activity. These include, butnot limited to, disease (e.g., cancers) that are characterized byamplification or overexpression of CCNE1 such as ovarian cancer, uterinecarcinosarcoma and breast cancer and p27 inactivation such as breastcancer and melanomas. Accordingly, in some embodiments of the methods,the patient has been previously determined to have an amplification ofthe cyclin E1 (CCNE1) gene and/or an expression level of CCNE1 in abiological sample obtained from the human subject that is higher than acontrol expression level of CCNE1. Alternatively, a compound of Formula(I) or of any of the formulas as described herein, or a compound asrecited in any of the claims and described herein, or a salt orstereoisomer thereof, can be used in conjunction with other agents orstandard cancer treatments, as described below. In one embodiment, thepresent disclosure provides a method for inhibiting growth of tumorcells in vitro. The method includes contacting the tumor cells in vitrowith a compound of Formula (I) or of any of the formulas as describedherein, or of a compound as recited in any of the claims and describedherein, or of a salt or stereoisomer thereof. In another embodiment, thepresent disclosure provides a method for inhibiting growth of tumorcells with CCNE1 amplification and overexpression in an individual or apatient. The method includes administering to the individual or patientin need thereof a therapeutically effective amount of a compound ofFormula (I) or of any of the formulas as described herein, or of acompound as recited in any of the claims and described herein, or a saltor a stereoisomer thereof.

In some embodiments, provided herein is a method of inhibiting CDK2,comprising contacting the CDK2 with a compound of Formula (I) or any ofthe formulas as described herein, a compound as recited in any of theclaims and described herein, or a salt thereof. In some embodiments,provided herein is a method of inhibiting CDK2 in a patient, comprisingadministering to the patient a compound of Formula (I) or any of theformulas as described herein, a compound as recited in any of the claimsand described herein, or a salt thereof.

In some embodiments, provided herein is a method for treating cancer.The method includes administering to a patient (in need thereof), atherapeutically effective amount of a compound of Formula (I) or any ofthe formulas as described herein, a compound as recited in any of theclaims and described herein, or a salt thereof. In another embodiment,the cancer is characterized by amplification or overexpression of CCNE1.In some embodiments, the cancer is ovarian cancer or breast cancer,characterized by amplification or overexpression of CCNE1.

In some embodiments, provided herein is a method of treating a diseaseor disorder associated with CDK2 in a patient, comprising administeringto the patient a therapeutically effective amount of a compound ofFormula (I) or any of the formulas as described herein, a compound asrecited in any of the claims and described herein, or a salt thereof. Insome embodiments, the disease or disorder associated with CDK2 isassociated with an amplification of the cyclin E1 (CCNE1) gene and/oroverexpression of CCNE1.

In some embodiments, the disease or disorder associated with CDK2 isN-myc amplified neuroblastoma cells (See Molenaar et al., Proc Natl AcadSci USA, 106(31):12968-12973), K-Ras mutant lung cancers (see Hu, S., etal., Mol Cancer Ther, 2015, 14(11):2576-85, and cancers with FBW7mutation and CCNE1 overexpression (see Takada, et al., Cancer Res, 2017,77(18):4881-4893).

In some embodiments, the disease or disorder associated with CDK2 islung squamous cell carcinoma, lung adenocarcinoma, pancreaticadenocarcinoma, breast invasive carcinoma, uterine carcinosarcoma,ovarian serous cystadenocarcinoma, stomach adenocarcinoma, esophagealcarcinoma, bladder urothelial carcinoma, mesothelioma, or sarcoma.

In some embodiments, the disease or disorder associated with CDK2 islung adenocarcinoma, breast invasive carcinoma, uterine carcinosarcoma,ovarian serous cystadenocarcinoma, or stomach adenocarcinoma.

In some embodiments, the disease or disorder associated with CDK2 is anadenocarcinoma, carcinoma, or cystadenocarcinoma.

In some embodiments, the disease or disorder associated with CDK2 isuterine cancer, ovarian cancer, stomach cancer, esophageal cancer, lungcancer, bladder cancer, pancreatic cancer, or breast cancer.

In some embodiments, the disease or disorder associated with CDK2 is acancer.

In some embodiments, the cancer is characterized by amplification oroverexpression of CCNE1. In some embodiments, the cancer is ovariancancer or breast cancer, characterized by amplification oroverexpression of CCNE1.

In some embodiments, the breast cancer is chemotherapy or radiotherapyresistant breast cancer, endocrine resistant breast cancer, trastuzumabresistant breast cancer, or breast cancer demonstrating primary oracquired resistance to CDK4/6 inhibition. In some embodiments, thebreast cancer is advanced or metastatic breast cancer.

Examples of cancers that are treatable using the compounds of thepresent disclosure include, but are not limited to, bone cancer,pancreatic cancer, skin cancer, cancer of the head or neck, cutaneous orintraocular malignant melanoma, uterine cancer, ovarian cancer, rectalcancer, cancer of the anal region, stomach cancer, testicular cancer,uterine cancer, carcinoma of the fallopian tubes, carcinoma of theendometrium, endometrial cancer, carcinoma of the cervix, carcinoma ofthe vagina, carcinoma of the vulva, Hodgkin's Disease, non-Hodgkin'slymphoma, cancer of the esophagus, cancer of the small intestine, cancerof the endocrine system, cancer of the thyroid gland, cancer of theparathyroid gland, cancer of the adrenal gland, sarcoma of soft tissue,cancer of the urethra, cancer of the penis, chronic or acute leukemiasincluding acute myeloid leukemia, chronic myeloid leukemia, acutelymphoblastic leukemia, chronic lymphocytic leukemia, solid tumors ofchildhood, lymphocytic lymphoma, cancer of the bladder, cancer of thekidney or urethra, carcinoma of the renal pelvis, neoplasm of thecentral nervous system (CNS), primary CNS lymphoma, tumor angiogenesis,spinal axis tumor, brain stem glioma, pituitary adenoma, Kaposi'ssarcoma, epidermoid cancer, squamous cell cancer, T-cell lymphoma,environmentally induced cancers including those induced by asbestos, andcombinations of said cancers. The compounds of the present disclosureare also useful for the treatment of metastatic cancers.

In some embodiments, cancers treatable with compounds of the presentdisclosure include melanoma (e.g., metastatic malignant melanoma, BRAFand HSP90 inhibition-resistant melanoma), renal cancer (e.g., clear cellcarcinoma), prostate cancer (e.g., hormone refractory prostateadenocarcinoma), breast cancer, colon cancer, lung cancer (e.g.,non-small cell lung cancer and small cell lung cancer), squamous cellhead and neck cancer, urothelial cancer (e.g., bladder) and cancers withhigh microsatellite instability (MSI^(high)). Additionally, thedisclosure includes refractory or recurrent malignancies whose growthmay be inhibited using the compounds of the disclosure.

In some embodiments, cancers that are treatable using the compounds ofthe present disclosure include, but are not limited to, solid tumors(e.g., prostate cancer, colon cancer, esophageal cancer, endometrialcancer, ovarian cancer, uterine cancer, renal cancer, hepatic cancer,pancreatic cancer, gastric cancer, breast cancer, lung cancer, cancersof the head and neck, thyroid cancer, glioblastoma, sarcoma, bladdercancer, etc.), hematological cancers (e.g., lymphoma, leukemia such asacute lymphoblastic leukemia (ALL), acute myelogenous leukemia (AML),chronic lymphocytic leukemia (CLL), chronic myelogenous leukemia (CML),DLBCL, mantle cell lymphoma, Non-Hodgkin lymphoma (including relapsed orrefractory NHL and recurrent follicular), Hodgkin lymphoma or multiplemyeloma) and combinations of said cancers.

In some embodiments, cancers that are treatable using the compounds ofthe present disclosure include, but are not limited to,cholangiocarcinoma, bile duct cancer, triple negative breast cancer,rhabdomyosarcoma, small cell lung cancer, leiomyosarcoma, hepatocellularcarcinoma, Ewing's sarcoma, brain cancer, brain tumor, astrocytoma,neuroblastoma, neurofibroma, basal cell carcinoma, chondrosarcoma,epithelioid sarcoma, eye cancer, Fallopian tube cancer, gastrointestinalcancer, gastrointestinal stromal tumors, hairy cell leukemia, intestinalcancer, islet cell cancer, oral cancer, mouth cancer, throat cancer,laryngeal cancer, lip cancer, mesothelioma, neck cancer, nasal cavitycancer, ocular cancer, ocular melanoma, pelvic cancer, rectal cancer,renal cell carcinoma, salivary gland cancer, sinus cancer, spinalcancer, tongue cancer, tubular carcinoma, urethral cancer, and ureteralcancer.

In some embodiments, the compounds of the present disclosure can be usedto treat sickle cell disease and sickle cell anemia.

In some embodiments, diseases and indications that are treatable usingthe compounds of the present disclosure include, but are not limited tohematological cancers, sarcomas, lung cancers, gastrointestinal cancers,genitourinary tract cancers, liver cancers, bone cancers, nervous systemcancers, gynecological cancers, and skin cancers.

Exemplary hematological cancers include lymphomas and leukemias such asacute lymphoblastic leukemia (ALL), acute myelogenous leukemia (AML),acute promyelocytic leukemia (APL), chronic lymphocytic leukemia (CLL),chronic myelogenous leukemia (CML), diffuse large B-cell lymphoma(DLBCL), mantle cell lymphoma, Non-Hodgkin lymphoma (including relapsedor refractory NHL and recurrent follicular), Hodgkin lymphoma,myeloproliferative diseases (e.g., primary myelofibrosis (PMF),polycythemia vera (PV), and essential thrombocytosis (ET)),myelodysplasia syndrome (MDS), T-cell acute lymphoblastic lymphoma(T-ALL) and multiple myeloma (MM).

Exemplary sarcomas include chondrosarcoma, Ewing's sarcoma,osteosarcoma, rhabdomyosarcoma, angiosarcoma, fibrosarcoma, liposarcoma,myxoma, rhabdomyoma, rhabdosarcoma, fibroma, lipoma, harmatoma, andteratoma.

Exemplary lung cancers include non-small cell lung cancer (NSCLC), smallcell lung cancer (SCLC), bronchogenic carcinoma, squamous cell,undifferentiated small cell, undifferentiated large cell,adenocarcinoma, alveolar (bronchiolar) carcinoma, bronchial adenoma,chondromatous hamartoma, and mesothelioma.

Exemplary gastrointestinal cancers include cancers of the esophagus(squamous cell carcinoma, adenocarcinoma, leiomyosarcoma, lymphoma),stomach (carcinoma, lymphoma, leiomyosarcoma), pancreas (ductaladenocarcinoma, insulinoma, glucagonoma, gastrinoma, carcinoid tumors,vipoma), small bowel (adenocarcinoma, lymphoma, carcinoid tumors,Kaposi's sarcoma, leiomyoma, hemangioma, lipoma, neurofibroma, fibroma),large bowel (adenocarcinoma, tubular adenoma, villous adenoma,hamartoma, leiomyoma), and colorectal cancer.

Exemplary genitourinary tract cancers include cancers of the kidney(adenocarcinoma, Wilm's tumor [nephroblastoma]), bladder and urethra(squamous cell carcinoma, transitional cell carcinoma, adenocarcinoma),prostate (adenocarcinoma, sarcoma), and testis (seminoma, teratoma,embryonal carcinoma, teratocarcinoma, choriocarcinoma, sarcoma,interstitial cell carcinoma, fibroma, fibroadenoma, adenomatoid tumors,lipoma).

Exemplary liver cancers include hepatoma (hepatocellular carcinoma),cholangiocarcinoma, hepatoblastoma, angiosarcoma, hepatocellularadenoma, and hemangioma.

Exemplary bone cancers include, for example, osteogenic sarcoma(osteosarcoma), fibrosarcoma, malignant fibrous histiocytoma,chondrosarcoma, Ewing's sarcoma, malignant lymphoma (reticulum cellsarcoma), multiple myeloma, malignant giant cell tumor chordoma,osteochronfroma (osteocartilaginous exostoses), benign chondroma,chondroblastoma, chondromyxofibroma, osteoid osteoma, and giant celltumors

Exemplary nervous system cancers include cancers of the skull (osteoma,hemangioma, granuloma, xanthoma, osteitis deformans), meninges(meningioma, meningiosarcoma, gliomatosis), brain (astrocytoma,medulloblastoma, glioma, ependymoma, germinoma (pinealoma),glioblastoma, glioblastoma multiform, oligodendroglioma, schwannoma,retinoblastoma, congenital tumors), and spinal cord (neurofibroma,meningioma, glioma, sarcoma), as well as neuroblastoma andLhermitte-Duclos disease.

Exemplary gynecological cancers include cancers of the uterus(endometrial carcinoma), cervix (cervical carcinoma, pre-tumor cervicaldysplasia), ovaries (ovarian carcinoma (serous cystadenocarcinoma,mucinous cystadenocarcinoma, unclassified carcinoma), granulosa-thecalcell tumors, Sertoli-Leydig cell tumors, dysgerminoma, malignantteratoma), vulva (squamous cell carcinoma, intraepithelial carcinoma,adenocarcinoma, fibrosarcoma, melanoma), vagina (clear cell carcinoma,squamous cell carcinoma, botryoid sarcoma (embryonal rhabdomyosarcoma),and fallopian tubes (carcinoma).

Exemplary skin cancers include melanoma, basal cell carcinoma, Merkelcell carcinoma, squamous cell carcinoma, Kaposi's sarcoma, molesdysplastic nevi, lipoma, angioma, dermatofibroma, and keloids. In someembodiments, diseases and indications that are treatable using thecompounds of the present disclosure include, but are not limited to,sickle cell disease (e.g., sickle cell anemia), triple-negative breastcancer (TNBC), myelodysplastic syndromes, testicular cancer, bile ductcancer, esophageal cancer, and urothelial carcinoma.

It is believed that compounds of Formula (I), or any of the embodimentsthereof, may possess satisfactory pharmacological profile and promisingbiopharmaceutical properties, such as toxicological profile, metabolismand pharmacokinetic properties, solubility, and permeability. It will beunderstood that determination of appropriate biopharmaceuticalproperties is within the knowledge of a person skilled in the art, e.g.,determination of cytotoxicity in cells or inhibition of certain targetsor channels to determine potential toxicity.

The terms “individual” or “patient,” used interchangeably, refer to anyanimal, including mammals, preferably mice, rats, other rodents,rabbits, dogs, cats, swine, cattle, sheep, horses, or primates, and mostpreferably humans.

The phrase “therapeutically effective amount” refers to the amount ofactive compound or pharmaceutical agent that elicits the biological ormedicinal response in a tissue, system, animal, individual or human thatis being sought by a researcher, veterinarian, medical doctor or otherclinician.

As used herein, the term “treating” or “treatment” refers to one or moreof (1) inhibiting the disease; e.g., inhibiting a disease, condition ordisorder in an individual who is experiencing or displaying thepathology or symptomatology of the disease, condition or disorder (i.e.,arresting further development of the pathology and/or symptomatology);and (2) ameliorating the disease; e.g., ameliorating a disease,condition or disorder in an individual who is experiencing or displayingthe pathology or symptomatology of the disease, condition or disorder(i.e., reversing the pathology and/or symptomatology) such as decreasingthe severity of disease.

In some embodiments, the compounds of the invention are useful inpreventing or reducing the risk of developing any of the diseasesreferred to herein; e.g., preventing or reducing the risk of developinga disease, condition or disorder in an individual who may be predisposedto the disease, condition or disorder but does not yet experience ordisplay the pathology or symptomatology of the disease.

Combination Therapies I. Cancer Therapies

Cancer cell growth and survival can be impacted by dysfunction inmultiple signaling pathways. Thus, it is useful to combine differentenzyme/protein/receptor inhibitors, exhibiting different preferences inthe targets which they modulate the activities of, to treat suchconditions. Targeting more than one signaling pathway (or more than onebiological molecule involved in a given signaling pathway) may reducethe likelihood of drug-resistance arising in a cell population, and/orreduce the toxicity of treatment.

One or more additional pharmaceutical agents such as, for example,chemotherapeutics, anti-inflammatory agents, steroids,immunosuppressants, immune-oncology agents, metabolic enzyme inhibitors,chemokine receptor inhibitors, and phosphatase inhibitors, as well astargeted therapies such as Bcr-Abl, Flt-3, EGFR, HER2, JAK, c-MET,VEGFR, PDGFR, c-Kit, IGF-1R, RAF, FAK, and CDK4/6 kinase inhibitors suchas, for example, those described in WO 2006/056399 can be used incombination with the compounds of the present disclosure for treatmentof CDK2-associated diseases, disorders or conditions. Other agents suchas therapeutic antibodies can be used in combination with the compoundsof the present disclosure for treatment of CDK2-associated diseases,disorders or conditions. The one or more additional pharmaceuticalagents can be administered to a patient simultaneously or sequentially.

In some embodiments, the CDK2 inhibitor is administered or used incombination with a BCL2 inhibitor or a CDK4/6 inhibitor.

The compounds as disclosed herein can be used in combination with one ormore other enzyme/protein/receptor inhibitors therapies for thetreatment of diseases, such as cancer and other diseases or disordersdescribed herein. Examples of diseases and indications treatable withcombination therapies include those as described herein. Examples ofcancers include solid tumors and non-solid tumors, such as liquidtumors, blood cancers. Examples of infections include viral infections,bacterial infections, fungus infections or parasite infections. Forexample, the compounds of the present disclosure can be combined withone or more inhibitors of the following kinases for the treatment ofcancer: Akt1, Akt2, Akt3, BCL2, CDK4/6, TGF-DR, PKA, PKG, PKC,CaM-kinase, phosphorylase kinase, MEKK, ERK, MAPK, mTOR^(a1), EGFR,HER2, HER3, HER4, INS-R, IDH2, IGF-1R, IR-R, PDGFαR, PDGFβR, PI3K(alpha, beta, gamma, delta, and multiple or selective), CSF1R, KIT,FLK-II, KDR/FLK-1, FLK-4, fit-1, FGFR1, FGFR2, FGFR3, FGFR4, c-Met,PARP, Ron, Sea, TRKA, TRKB, TRKC, TAM kinases (Axl, Mer, Tyro3), FLT3,VEGFR/Flt2, Flt4, EphA1, EphA2, EphA3, EphB2, EphB4, Tie2, Src, Fyn,Lck, Fgr, Btk, Fak, SYK, FRK, JAK, ABL, ALK and B-Raf. In someembodiments, the compounds of the present disclosure can be combinedwith one or more of the following inhibitors for the treatment of canceror infections. Non-limiting examples of inhibitors that can be combinedwith the compounds of the present disclosure for treatment of cancer andinfections include an FGFR inhibitor (FGFR1, FGFR2, FGFR3 or FGFR4,e.g., pemigatinib (INCB54828), INCB62079), an EGFR inhibitor (also knownas ErB-1 or HER-1; e.g., erlotinib, gefitinib, vandetanib, orsimertinib,cetuximab, necitumumab, or panitumumab), a VEGFR inhibitor or pathwayblocker (e.g., bevacizumab, pazopanib, sunitinib, sorafenib, axitinib,regorafenib, ponatinib, cabozantinib, vandetanib, ramucirumab,lenvatinib, ziv-aflibercept), a PARP inhibitor (e.g., olaparib,rucaparib, veliparib or niraparib), a JAK inhibitor (JAK1 and/or JAK2,e.g., ruxolitinib or baricitinib; JAK1, e.g., itacitinib (INCB39110),INCB052793, or INCB054707), an IDO inhibitor (e.g., epacadostat, NLG919,or BMS-986205, MK7162), an LSD1 inhibitor (e.g., GSK2979552, INCB59872and INCB60003), a TDO inhibitor, a PI3K-delta inhibitor (e.g.,parsaclisib (INCB50465) or INCB50797), a PI3K-gamma inhibitor such asPI3K-gamma selective inhibitor, a Pim inhibitor (e.g., INCB53914), aCSF1R inhibitor, a TAM receptor tyrosine kinases (Tyro-3, Axl, and Mer;e.g., INCB081776), an adenosine receptor antagonist (e.g., A2a/A2breceptor antagonist), an HPK1 inhibitor, a chemokine receptor inhibitor(e.g., CCR2 or CCR5 inhibitor), a SHP1/2 phosphatase inhibitor, ahistone deacetylase inhibitor (HDAC) such as an HDAC8 inhibitor, anangiogenesis inhibitor, an interleukin receptor inhibitor, bromo andextra terminal family members inhibitors (for example, bromodomaininhibitors or BET inhibitors such as INCB54329 and INCB57643), c-METinhibitors (e.g., capmatinib), an anti-CD19 antibody (e.g.,tafasitamab), an ALK2 inhibitor (e.g., INCB00928); or combinationsthereof.

In some embodiments, the compound or salt described herein isadministered with a PI3K6 inhibitor. In some embodiments, the compoundor salt described herein is administered with a JAK inhibitor. In someembodiments, the compound or salt described herein is administered witha JAK1 or JAK2 inhibitor (e.g., baricitinib or ruxolitinib). In someembodiments, the compound or salt described herein is administered witha JAK1 inhibitor. In some embodiments, the compound or salt describedherein is administered with a JAK1 inhibitor, which is selective overJAK2.

Example antibodies for use in combination therapy include, but are notlimited to, trastuzumab (e.g., anti-HER2), ranibizumab (e.g.,anti-VEGF-A), bevacizumab (AVASTIN™, e.g., anti-VEGF), panitumumab(e.g., anti-EGFR), cetuximab (e.g., anti-EGFR), rituxan (e.g.,anti-CD20), and antibodies directed to c-MET.

One or more of the following agents may be used in combination with thecompounds of the present disclosure and are presented as a non-limitinglist: a cytostatic agent, cisplatin, doxorubicin, taxotere, taxol,etoposide, irinotecan, camptosar, topotecan, paclitaxel, docetaxel,epothilones, tamoxifen, 5-fluorouracil, methotrexate, temozolomide,cyclophosphamide, SCH 66336, R115777, L778,123, BMS 214662, IRESSA™(gefitinib), TARCEVA™ (erlotinib), antibodies to EGFR, intron, ara-C,adriamycin, cytoxan, gemcitabine, uracil mustard, chlormethine,ifosfamide, melphalan, chlorambucil, pipobroman, triethylenemelamine,triethylenethiophosphoramine, busulfan, carmustine, lomustine,streptozocin, dacarbazine, floxuridine, cytarabine, 6-mercaptopurine,6-thioguanine, fludarabine phosphate, oxaliplatin, leucovirin, ELOXATIN™(oxaliplatin), pentostatine, vinblastine, vincristine, vindesine,bleomycin, dactinomycin, daunorubicin, doxorubicin, epirubicin,idarubicin, mithramycin, deoxycoformycin, mitomycin-C, L-asparaginase,teniposide 17.alpha.-ethinylestradiol, diethylstilbestrol, testosterone,Prednisone, Fluoxymesterone, Dromostanolone propionate, testolactone,megestrolacetate, methylprednisolone, methyltestosterone, prednisolone,triamcinolone, chlorotrianisene, hydroxyprogesterone, aminoglutethimide,estramustine, medroxyprogesteroneacetate, leuprolide, flutamide,toremifene, goserelin, carboplatin, hydroxyurea, amsacrine,procarbazine, mitotane, mitoxantrone, levamisole, navelbene,anastrazole, letrazole, capecitabine, reloxafine, droloxafine,hexamethylmelamine, avastin, HERCEPTIN™ (trastuzumab), BEXXAR™(tositumomab), VELCADE™ (bortezomib), ZEVALIN™ (ibritumomab tiuxetan),TRISENOX™ (arsenic trioxide), XELODA™ (capecitabine), vinorelbine,porfimer, ERBITUX™ (cetuximab), thiotepa, altretamine, melphalan,trastuzumab, lerozole, fulvestrant, exemestane, ifosfomide, rituximab,C225 (cetuximab), Campath (alemtuzumab), clofarabine, cladribine,aphidicolon, rituxan, sunitinib, dasatinib, tezacitabine, Smll,fludarabine, pentostatin, triapine, didox, trimidox, amidox, 3-AP, andMDL-101,731.

The compounds of the present disclosure can further be used incombination with other methods of treating cancers, for example bychemotherapy, irradiation therapy, tumor-targeted therapy, adjuvanttherapy, immunotherapy or surgery. Examples of immunotherapy includecytokine treatment (e.g., interferons, GM-CSF, G-CSF, IL-2), CRS-207immunotherapy, cancer vaccine, monoclonal antibody, bispecific ormulti-specific antibody, antibody drug conjugate, adoptive T celltransfer, Toll receptor agonists, RIG-I agonists, oncolytic virotherapyand immunomodulating small molecules, including thalidomide or JAK1/2inhibitor, PI3K6 inhibitor and the like. The compounds can beadministered in combination with one or more anti-cancer drugs, such asa chemotherapeutic agent. Examples of chemotherapeutics include any ofabarelix, aldesleukin, alemtuzumab, alitretinoin, allopurinol,altretamine, anastrozole, arsenic trioxide, asparaginase, azacitidine,bevacizumab, bexarotene, baricitinib, bleomycin, bortezomib, busulfanintravenous, busulfan oral, calusterone, capecitabine, carboplatin,carmustine, cetuximab, chlorambucil, cisplatin, cladribine, clofarabine,cyclophosphamide, cytarabine, dacarbazine, dactinomycin, dalteparinsodium, dasatinib, daunorubicin, decitabine, denileukin, denileukindiftitox, dexrazoxane, docetaxel, doxorubicin, dromostanolonepropionate, eculizumab, epirubicin, erlotinib, estramustine, etoposidephosphate, etoposide, exemestane, fentanyl citrate, filgrastim,floxuridine, fludarabine, fluorouracil, fulvestrant, gefitinib,gemcitabine, gemtuzumab ozogamicin, goserelin acetate, histrelinacetate, ibritumomab tiuxetan, idarubicin, ifosfamide, imatinibmesylate, interferon alfa 2a, irinotecan, lapatinib ditosylate,lenalidomide, letrozole, leucovorin, leuprolide acetate, levamisole,lomustine, meclorethamine, megestrol acetate, melphalan, mercaptopurine,methotrexate, methoxsalen, mitomycin C, mitotane, mitoxantrone,nandrolone phenpropionate, nelarabine, nofetumomab, oxaliplatin,paclitaxel, pamidronate, panitumumab, pegaspargase, pegfilgrastim,pemetrexed disodium, pentostatin, pipobroman, plicamycin, procarbazine,quinacrine, rasburicase, rituximab, ruxolitinib, sorafenib,streptozocin, sunitinib, sunitinib maleate, tamoxifen, temozolomide,teniposide, testolactone, thalidomide, thioguanine, thiotepa, topotecan,toremifene, tositumomab, trastuzumab, tretinoin, uracil mustard,valrubicin, vinblastine, vincristine, vinorelbine, vorinostat, andzoledronate.

Additional examples of chemotherapeutics include proteasome inhibitors(e.g., bortezomib), thalidomide, revlimid, and DNA-damaging agents suchas melphalan, doxorubicin, cyclophosphamide, vincristine, etoposide,carmustine, and the like.

Example steroids include corticosteroids such as dexamethasone orprednisone.

Example Bcr-Abl inhibitors include imatinib mesylate (GLEEVAC™),nilotinib, dasatinib, bosutinib, and ponatinib, and pharmaceuticallyacceptable salts. Other example suitable Bcr-Abl inhibitors include thecompounds, and pharmaceutically acceptable salts thereof, of the generaand species disclosed in U.S. Pat. No. 5,521,184, WO 04/005281, and U.S.Ser. No. 60/578,491.

Example suitable Flt-3 inhibitors include midostaurin, lestaurtinib,linifanib, sunitinib, sunitinib, maleate, sorafenib, quizartinib,crenolanib, pacritinib, tandutinib, PLX3397 and ASP2215, and theirpharmaceutically acceptable salts. Other example suitable Flt-3inhibitors include compounds, and their pharmaceutically acceptablesalts, as disclosed in WO 03/037347, WO 03/099771, and WO 04/046120.

Example suitable RAF inhibitors include dabrafenib, sorafenib, andvemurafenib, and their pharmaceutically acceptable salts. Other examplesuitable RAF inhibitors include compounds, and their pharmaceuticallyacceptable salts, as disclosed in WO 00/09495 and WO 05/028444.

Example suitable FAK inhibitors include VS-4718, VS-5095, VS-6062,VS-6063, BI853520, and GSK2256098, and their pharmaceutically acceptablesalts. Other example suitable FAK inhibitors include compounds, andtheir pharmaceutically acceptable salts, as disclosed in WO 04/080980,WO 04/056786, WO 03/024967, WO 01/064655, WO 00/053595, and WO01/014402.

Example suitable CDK4/6 inhibitors include palbociclib, ribociclib,trilaciclib, lerociclib, and abemaciclib, and their pharmaceuticallyacceptable salts. Other example suitable CDK4/6 inhibitors includecompounds, and their pharmaceutically acceptable salts, as disclosed inWO 09/085185, WO 12/129344, WO 11/101409, WO 03/062236, WO 10/075074,and WO 12/061156.

In some embodiments, the compounds of the disclosure can be used incombination with one or more other kinase inhibitors including imatinib,particularly for treating patients resistant to imatinib or other kinaseinhibitors.

In some embodiments, the compounds of the disclosure can be used incombination with a chemotherapeutic in the treatment of cancer, and mayimprove the treatment response as compared to the response to thechemotherapeutic agent alone, without exacerbation of its toxic effects.In some embodiments, the compounds of the disclosure can be used incombination with a chemotherapeutic provided herein. For example,additional pharmaceutical agents used in the treatment of multiplemyeloma, can include, without limitation, melphalan, melphalan plusprednisone [MP], doxorubicin, dexamethasone, and Velcade (bortezomib).Further additional agents used in the treatment of multiple myelomainclude Bcr-Abl, Flt-3, RAF and FAK kinase inhibitors. In someembodiments, the agent is an alkylating agent, a proteasome inhibitor, acorticosteroid, or an immunomodulatory agent. Examples of an alkylatingagent include cyclophosphamide (CY), melphalan (MEL), and bendamustine.In some embodiments, the proteasome inhibitor is carfilzomib. In someembodiments, the corticosteroid is dexamethasone (DEX). In someembodiments, the immunomodulatory agent is lenalidomide (LEN) orpomalidomide (POM). Additive or synergistic effects are desirableoutcomes of combining a CDK2 inhibitor of the present disclosure with anadditional agent.

The agents can be combined with the present compound in a single orcontinuous dosage form, or the agents can be administered simultaneouslyor sequentially as separate dosage forms.

The compounds of the present disclosure can be used in combination withone or more other inhibitors or one or more therapies for the treatmentof infections. Examples of infections include viral infections,bacterial infections, fungus infections or parasite infections.

In some embodiments, a corticosteroid such as dexamethasone isadministered to a patient in combination with the compounds of thedisclosure where the dexamethasone is administered intermittently asopposed to continuously.

The compounds of Formula (I) or any of the formulas as described herein,a compound as recited in any of the claims and described herein, orsalts thereof can be combined with another immunogenic agent, such ascancerous cells, purified tumor antigens (including recombinantproteins, peptides, and carbohydrate molecules), cells, and cellstransfected with genes encoding immune stimulating cytokines.Non-limiting examples of tumor vaccines that can be used includepeptides of melanoma antigens, such as peptides of gp100, MAGE antigens,Trp-2, MARTI and/or tyrosinase, or tumor cells transfected to expressthe cytokine GM-CSF.

The compounds of Formula (I) or any of the formulas as described herein,a compound as recited in any of the claims and described herein, orsalts thereof can be used in combination with a vaccination protocol forthe treatment of cancer. In some embodiments, the tumor cells aretransduced to express GM-CSF. In some embodiments, tumor vaccinesinclude the proteins from viruses implicated in human cancers such asHuman Papilloma Viruses (HPV), Hepatitis Viruses (HBV and HCV) andKaposi's Herpes Sarcoma Virus (KHSV). In some embodiments, the compoundsof the present disclosure can be used in combination with tumor specificantigen such as heat shock proteins isolated from tumor tissue itself.In some embodiments, the compounds of Formula (I) or any of the formulasas described herein, a compound as recited in any of the claims anddescribed herein, or salts thereof can be combined with dendritic cellsimmunization to activate potent anti-tumor responses.

The compounds of the present disclosure can be used in combination withbispecific macrocyclic peptides that target Fe alpha or Fe gammareceptor-expressing effectors cells to tumor cells. The compounds of thepresent disclosure can also be combined with macrocyclic peptides thatactivate host immune responsiveness.

In some further embodiments, combinations of the compounds of thedisclosure with other therapeutic agents can be administered to apatient prior to, during, and/or after a bone marrow transplant or stemcell transplant. The compounds of the present disclosure can be used incombination with bone marrow transplant for the treatment of a varietyof tumors of hematopoietic origin.

The compounds of Formula (I) or any of the formulas as described herein,a compound as recited in any of the claims and described herein, orsalts thereof can be used in combination with vaccines, to stimulate theimmune response to pathogens, toxins, and self-antigens. Examples ofpathogens for which this therapeutic approach may be particularlyuseful, include pathogens for which there is currently no effectivevaccine, or pathogens for which conventional vaccines are less thancompletely effective. These include, but are not limited to, HIV,Hepatitis (A, B, & C), Influenza, Herpes, Giardia, Malaria, Leishmania,Staphylococcus aureus, Pseudomonas Aeruginosa.

Viruses causing infections treatable by methods of the presentdisclosure include, but are not limit to human papillomavirus,influenza, hepatitis A, B, C or D viruses, adenovirus, poxvirus, herpessimplex viruses, human cytomegalovirus, severe acute respiratorysyndrome virus, Ebola virus, measles virus, herpes virus (e.g., VZV,HSV-1, HAV-6, HSV-II, and CMV, Epstein Barr virus), flaviviruses,echovirus, rhinovirus, coxsackie virus, cornovirus, respiratorysyncytial virus, mumps virus, rotavirus, measles virus, rubella virus,parvovirus, vaccinia virus, HTLV virus, dengue virus, papillomavirus,molluscum virus, poliovirus, rabies virus, JC virus and arboviralencephalitis virus.

Pathogenic bacteria causing infections treatable by methods of thedisclosure include, but are not limited to, chlamydia, rickettsialbacteria, mycobacteria, staphylococci, streptococci, pneumococci,meningococci and conococci, klebsiella, proteus, serratia, pseudomonas,legionella, diphtheria, salmonella, bacilli, cholera, tetanus, botulism,anthrax, plague, leptospirosis, and Lyme's disease bacteria.

Pathogenic fungi causing infections treatable by methods of thedisclosure include, but are not limited to, Candida (albicans, krusei,glabrata, tropicalis, etc.), Cryptococcus neoformans, Aspergillus(fumigatus, niger, etc.), Genus Mucorales (mucor, absidia, rhizophus),Sporothrix schenkii, Blastomyces dermatitidis, Paracoccidioidesbrasiliensis, Coccidioides immitis and Histoplasma capsulatum.

Pathogenic parasites causing infections treatable by methods of thedisclosure include, but are not limited to, Entamoeba histolytica,Balantidium coli, Naegleria fowleri, Acanthamoeba sp., Giardia lambia,Cryptosporidium sp., Pneumocystis carinii, Plasmodium vivax, Babesiamicroti, Trypanosoma brucei, Trypanosoma cruzi, Leishmania donovani,Toxoplasma gondi, and Nippostrongylus brasiliensis.

When more than one pharmaceutical agent is administered to a patient,they can be administered simultaneously, separately, sequentially, or incombination (e.g., for more than two agents).

Methods for the safe and effective administration of most of thesechemotherapeutic agents are known to those skilled in the art. Inaddition, their administration is described in the standard literature.For example, the administration of many of the chemotherapeutic agentsis described in the “Physicians' Desk Reference” (PDR, e.g., 1996edition, Medical Economics Company, Montvale, N.J.), the disclosure ofwhich is incorporated herein by reference as if set forth in itsentirety.

II. Immune-Checkpoint Therapies

Compounds of the present disclosure can be used in combination with oneor more immune checkpoint inhibitors for the treatment of diseases, suchas cancer or infections. Exemplary immune checkpoint inhibitors includeinhibitors against immune checkpoint molecules such as CBL-B, CD20,CD28, CD40, CD122, CD96, CD73, CD47, GITR, CSF1R, JAK, PI3K delta, PI3Kgamma, TAM, arginase, HPK1, CD137 (also known as 4-1B), ICOS, A2AR,B7-H3, B7-H4, BTLA, CTLA-4, LAG3, TIM3, TIGIT, CD112R, VISTA, PD-1,PD-L1 and PD-L2. In some embodiments, the immune checkpoint molecule isa stimulatory checkpoint molecule selected from CD27, CD28, CD40, ICOS,OX40, GITR and CD137. In some embodiments, the immune checkpointmolecule is an inhibitory checkpoint molecule selected from A2AR, B7-H3,B7-H4, BTLA, CTLA-4, IDO, KIR, LAG3, PD-1, TIM3, TIGIT, and VISTA. Insome embodiments, the compounds provided herein can be used incombination with one or more agents selected from KIR inhibitors, TIGITinhibitors, LAIR1 inhibitors, CD160 inhibitors, 2B4 inhibitors and TGFRbeta inhibitors.

In some embodiments, the compounds provided herein can be used incombination with one or more agonists of immune checkpoint molecules,e.g., OX40, CD27, GITR, and CD137 (also known as 4-1B).

In some embodiments, the inhibitor of an immune checkpoint molecule isanti-PD1 antibody, anti-PD-L1 antibody, or anti-CTLA-4 antibody.

In some embodiments, the inhibitor of an immune checkpoint molecule isan inhibitor of PD-1, e.g., an anti-PD-1 monoclonal antibody. In someembodiments, the anti-PD-1 monoclonal antibody is nivolumab,pembrolizumab (also known as MK-3475), pidilizumab, SHR-1210, PDR001,MGA012, PDR001, AB122, or AMP-224. In some embodiments, the anti-PD-1monoclonal antibody is nivolumab or pembrolizumab. In some embodiments,the anti-PD1 antibody is pembrolizumab. In some embodiments, theanti-PD-1 monoclonal antibody is MGA012. In some embodiments, theanti-PD1 antibody is SHR-1210. Other anti-cancer agent(s) includeantibody therapeutics such as 4-1BB (e.g., urelumab, utomilumab).

In some embodiments, the inhibitor of an immune checkpoint molecule isan inhibitor of PD-L1, e.g., an anti-PD-L1 monoclonal antibody. In someembodiments, the anti-PD-L1 monoclonal antibody is BMS-935559, MEDI4736,MPDL3280A (also known as RG7446), or MSB0010718C. In some embodiments,the anti-PD-L1 monoclonal antibody is MPDL3280A or MEDI4736. In someembodiments, the inhibitor of an immune checkpoint molecule is aninhibitor of PD-1 and PD-L1, e.g., an anti-PD-1/PD-L1 bispecificantibody. In some embodiments, the anti-PD-1/PD-L1 is MCLA-136.

In some embodiments, the inhibitor is MCLA-145.

In some embodiments, the inhibitor of an immune checkpoint molecule isan inhibitor of CTLA-4, e.g., an anti-CTLA-4 antibody. In someembodiments, the anti-CTLA-4 antibody is ipilimumab, tremelimumab,AGEN1884, or CP-675,206.

In some embodiments, the inhibitor of an immune checkpoint molecule isan inhibitor of LAG3, e.g., an anti-LAG3 antibody. In some embodiments,the anti-LAG3 antibody is BMS-986016, LAG525, or INCAGN2385.

In some embodiments, the inhibitor of an immune checkpoint molecule isan inhibitor of TIM3, e.g., an anti-TIM3 antibody. In some embodiments,the anti-TIM3 antibody is INCAGN2390, MBG453, or TSR-022.

In some embodiments, the inhibitor of an immune checkpoint molecule isan inhibitor of GITR, e.g., an anti-GITR antibody. In some embodiments,the anti-GITR antibody is TRX518, MK-4166, INCAGN1876, MK-1248, AMG228,BMS-986156, GWN323, or MEDI1873.

In some embodiments, the inhibitor of an immune checkpoint molecule isan agonist of OX40, e.g., OX40 agonist antibody or OX40L fusion protein.In some embodiments, the anti-OX40 antibody is MEDI0562, MOXR-0916,PF-04518600, GSK3174998, or BMS-986178. In some embodiments, the OX40Lfusion protein is MEDI6383.

In some embodiments, the inhibitor of an immune checkpoint molecule isan inhibitor of CD20, e.g., an anti-CD20 antibody. In some embodiments,the anti-CD20 antibody is obinutuzumab or rituximab.

The compounds of the present disclosure can be used in combination withbispecific antibodies. In some embodiments, one of the domains of thebispecific antibody targets PD-1, PD-L1, CTLA-4, GITR, OX40, TIM3, LAG3,CD137, ICOS, CD3 or TGFβ receptor.

In some embodiments, the compounds of the disclosure can be used incombination with one or more metabolic enzyme inhibitors. In someembodiments, the metabolic enzyme inhibitor is an inhibitor of IDO1,TDO, or arginase. Examples of IDO1 inhibitors include epacadostat,NLG919, BMS-986205, PF-06840003, IOM2983, RG-70099 and LY338196.

As provided throughout, the additional compounds, inhibitors, agents,etc. can be combined with the present compound in a single or continuousdosage form, or they can be administered simultaneously or sequentiallyas separate dosage forms.

Pharmaceutical Formulations and Dosage Forms

When employed as pharmaceuticals, the compounds of the disclosure can beadministered in the form of pharmaceutical compositions. Thesecompositions can be prepared in a manner well known in thepharmaceutical art, and can be administered by a variety of routes,depending upon whether local or systemic treatment is desired and uponthe area to be treated. Administration may be topical (includingtransdermal, epidermal, ophthalmic and to mucous membranes includingintranasal, vaginal and rectal delivery), pulmonary (e.g., by inhalationor insufflation of powders or aerosols, including by nebulizer;intratracheal or intranasal), oral, or parenteral. Parenteraladministration includes intravenous, intraarterial, subcutaneous,intraperitoneal intramuscular or injection or infusion; or intracranial,e.g., intrathecal or intraventricular, administration.

Parenteral administration can be in the form of a single bolus dose, ormay be, for example, by a continuous perfusion pump. Pharmaceuticalcompositions and formulations for topical administration may includetransdermal patches, ointments, lotions, creams, gels, drops,suppositories, sprays, liquids and powders. Conventional pharmaceuticalcarriers, aqueous, powder or oily bases, thickeners and the like may benecessary or desirable.

This disclosure also includes pharmaceutical compositions which contain,as the active ingredient, the compound of the disclosure or apharmaceutically acceptable salt thereof, in combination with one ormore pharmaceutically acceptable carriers (excipients). In someembodiments, the composition is suitable for topical administration. Inmaking the compositions of the disclosure, the active ingredient istypically mixed with an excipient, diluted by an excipient or enclosedwithin such a carrier in the form of, for example, a capsule, sachet,paper, or other container. When the excipient serves as a diluent, itcan be a solid, semi-solid, or liquid material, which acts as a vehicle,carrier or medium for the active ingredient. Thus, the compositions canbe in the form of tablets, pills, powders, lozenges, sachets, cachets,elixirs, suspensions, emulsions, solutions, syrups, aerosols (as a solidor in a liquid medium), ointments containing, for example, up to 10% byweight of the active compound, soft and hard gelatin capsules,suppositories, sterile injectable solutions, and sterile packagedpowders.

In preparing a formulation, the active compound can be milled to providethe appropriate particle size prior to combining with the otheringredients. If the active compound is substantially insoluble, it canbe milled to a particle size of less than 200 mesh. If the activecompound is substantially water soluble, the particle size can beadjusted by milling to provide a substantially uniform distribution inthe formulation, e.g. about 40 mesh.

The compounds of the disclosure may be milled using known millingprocedures such as wet milling to obtain a particle size appropriate fortablet formation and for other formulation types. Finely divided(nanoparticulate) preparations of the compounds of the disclosure can beprepared by processes known in the art, e.g., see International App. No.WO 2002/000196.

Some examples of suitable excipients include lactose, dextrose, sucrose,sorbitol, mannitol, starches, gum acacia, calcium phosphate, alginates,tragacanth, gelatin, calcium silicate, microcrystalline cellulose,polyvinylpyrrolidone, cellulose, water, syrup, and methyl cellulose. Theformulations can additionally include: lubricating agents such as talc,magnesium stearate, and mineral oil; wetting agents; emulsifying andsuspending agents; preserving agents such as methyl- andpropylhydroxy-benzoates; sweetening agents; and flavoring agents. Thecompositions of the disclosure can be formulated so as to provide quick,sustained or delayed release of the active ingredient afteradministration to the patient by employing procedures known in the art.

The compositions can be formulated in a unit dosage form, each dosagecontaining from about 5 to about 1000 mg (1 g), more usually about 100to about 500 mg, of the active ingredient. The term “unit dosage forms”refers to physically discrete units suitable as unitary dosages forhuman subjects and other mammals, each unit containing a predeterminedquantity of active material calculated to produce the desiredtherapeutic effect, in association with a suitable pharmaceuticalexcipient.

In some embodiments, the compositions of the disclosure contain fromabout 5 to about 50 mg of the active ingredient. One having ordinaryskill in the art will appreciate that this embodies compositionscontaining about 5 to about 10, about 10 to about 15, about 15 to about20, about 20 to about 25, about 25 to about 30, about 30 to about 35,about 35 to about 40, about 40 to about 45, or about 45 to about 50 mgof the active ingredient.

In some embodiments, the compositions of the disclosure contain fromabout 50 to about 500 mg of the active ingredient. One having ordinaryskill in the art will appreciate that this embodies compositionscontaining about 50 to about 100, about 100 to about 150, about 150 toabout 200, about 200 to about 250, about 250 to about 300, about 350 toabout 400, or about 450 to about 500 mg of the active ingredient.

In some embodiments, the compositions of the disclosure contain fromabout 500 to about 1000 mg of the active ingredient. One having ordinaryskill in the art will appreciate that this embodies compositionscontaining about 500 to about 550, about 550 to about 600, about 600 toabout 650, about 650 to about 700, about 700 to about 750, about 750 toabout 800, about 800 to about 850, about 850 to about 900, about 900 toabout 950, or about 950 to about 1000 mg of the active ingredient.

Similar dosages may be used of the compounds described herein in themethods and uses of the disclosure.

The active compound can be effective over a wide dosage range and isgenerally administered in a pharmaceutically effective amount. It willbe understood, however, that the amount of the compound actuallyadministered will usually be determined by a physician, according to therelevant circumstances, including the condition to be treated, thechosen route of administration, the actual compound administered, theage, weight, and response of the individual patient, the severity of thepatient's symptoms, and the like.

For preparing solid compositions such as tablets, the principal activeingredient is mixed with a pharmaceutical excipient to form a solidpreformulation composition containing a homogeneous mixture of acompound of the present disclosure. When referring to thesepreformulation compositions as homogeneous, the active ingredient istypically dispersed evenly throughout the composition so that thecomposition can be readily subdivided into equally effective unit dosageforms such as tablets, pills and capsules. This solid preformulation isthen subdivided into unit dosage forms of the type described abovecontaining from, for example, about 0.1 to about 1000 mg of the activeingredient of the present disclosure.

The tablets or pills of the present disclosure can be coated orotherwise compounded to provide a dosage form affording the advantage ofprolonged action. For example, the tablet or pill can comprise an innerdosage and an outer dosage component, the latter being in the form of anenvelope over the former. The two components can be separated by anenteric layer which serves to resist disintegration in the stomach andpermit the inner component to pass intact into the duodenum or to bedelayed in release. A variety of materials can be used for such entericlayers or coatings, such materials including a number of polymeric acidsand mixtures of polymeric acids with such materials as shellac, cetylalcohol, and cellulose acetate.

The liquid forms in which the compounds and compositions of the presentdisclosure can be incorporated for administration orally or by injectioninclude aqueous solutions, suitably flavored syrups, aqueous or oilsuspensions, and flavored emulsions with edible oils such as cottonseedoil, sesame oil, coconut oil, or peanut oil, as well as elixirs andsimilar pharmaceutical vehicles.

Compositions for inhalation or insufflation include solutions andsuspensions in pharmaceutically acceptable, aqueous or organic solvents,or mixtures thereof, and powders. The liquid or solid compositions maycontain suitable pharmaceutically acceptable excipients as describedsupra. In some embodiments, the compositions are administered by theoral or nasal respiratory route for local or systemic effect.Compositions can be nebulized by use of inert gases. Nebulized solutionsmay be breathed directly from the nebulizing device or the nebulizingdevice can be attached to a face mask, tent, or intermittent positivepressure breathing machine. Solution, suspension, or powder compositionscan be administered orally or nasally from devices which deliver theformulation in an appropriate manner.

Topical formulations can contain one or more conventional carriers. Insome embodiments, ointments can contain water and one or morehydrophobic carriers selected from, for example, liquid paraffin,polyoxyethylene alkyl ether, propylene glycol, white Vaseline, and thelike. Carrier compositions of creams can be based on water incombination with glycerol and one or more other components, e.g.,glycerinemonostearate, PEG-glycerinemonostearate and cetylstearylalcohol. Gels can be formulated using isopropyl alcohol and water,suitably in combination with other components such as, for example,glycerol, hydroxyethyl cellulose, and the like. In some embodiments,topical formulations contain at least about 0.1, at least about 0.25, atleast about 0.5, at least about 1, at least about 2, or at least about 5wt % of the compound of the disclosure. The topical formulations can besuitably packaged in tubes of, for example, 100 g which are optionallyassociated with instructions for the treatment of the select indication,e.g., psoriasis or other skin condition.

The amount of compound or composition administered to a patient willvary depending upon what is being administered, the purpose of theadministration, such as prophylaxis or therapy, the state of thepatient, the manner of administration, and the like. In therapeuticapplications, compositions can be administered to a patient alreadysuffering from a disease in an amount sufficient to cure or at leastpartially arrest the symptoms of the disease and its complications.Effective doses will depend on the disease condition being treated aswell as by the judgment of the attending clinician depending uponfactors such as the severity of the disease, the age, weight and generalcondition of the patient, and the like.

The compositions administered to a patient can be in the form ofpharmaceutical compositions described above. These compositions can besterilized by conventional sterilization techniques, or may be sterilefiltered. Aqueous solutions can be packaged for use as is, orlyophilized, the lyophilized preparation being combined with a sterileaqueous carrier prior to administration. The pH of the compoundpreparations typically will be between 3 and 11, more preferably from 5to 9 and most preferably from 7 to 8. It will be understood that use ofcertain of the foregoing excipients, carriers, or stabilizers willresult in the formation of pharmaceutical salts.

The therapeutic dosage of a compound of the present disclosure can varyaccording to, for example, the particular use for which the treatment ismade, the manner of administration of the compound, the health andcondition of the patient, and the judgment of the prescribing physician.The proportion or concentration of a compound of the disclosure in apharmaceutical composition can vary depending upon a number of factorsincluding dosage, chemical characteristics (e.g., hydrophobicity), andthe route of administration. For example, the compounds of thedisclosure can be provided in an aqueous physiological buffer solutioncontaining about 0.1 to about 10% w/v of the compound for parenteraladministration. Some typical dose ranges are from about 1 μg/kg to about1 g/kg of body weight per day. In some embodiments, the dose range isfrom about 0.01 mg/kg to about 100 mg/kg of body weight per day. Thedosage is likely to depend on such variables as the type and extent ofprogression of the disease or disorder, the overall health status of theparticular patient, the relative biological efficacy of the compoundselected, formulation of the excipient, and its route of administration.Effective doses can be extrapolated from dose-response curves derivedfrom in vitro or animal model test systems.

The compositions of the disclosure can further include one or moreadditional pharmaceutical agents such as a chemotherapeutic, steroid,anti-inflammatory compound, or immunosuppressant, examples of which arelisted herein.

Labeled Compounds and Assay Methods

Another aspect of the present disclosure relates to labeled compounds ofthe disclosure (radio-labeled, fluorescent-labeled, etc.) that would beuseful not only in imaging techniques but also in assays, both in vitroand in vivo, for localizing and quantitating CDK2 in tissue samples,including human, and for identifying CDK2 activators by inhibitionbinding of a labeled compound. Substitution of one or more of the atomsof the compounds of the present disclosure can also be useful ingenerating differentiated ADME (Adsorption, Distribution, Metabolism andExcretion.) Accordingly, the present disclosure includes CDK2 assaysthat contain such labeled or substituted compounds.

The present disclosure further includes isotopically-labeled compoundsof the disclosure. An “isotopically” or “radio-labeled” compound is acompound of the disclosure where one or more atoms are replaced orsubstituted by an atom having an atomic mass or mass number differentfrom the atomic mass or mass number typically found in nature (i.e.,naturally occurring). Suitable radionuclides that may be incorporated incompounds of the present disclosure include but are not limited to ²H(also written as D for deuterium), ³H (also written as T for tritium),¹¹C, ¹³C, ¹⁴C, ¹³N, ¹⁵N, ¹⁵O, ¹⁷O, ¹⁸O, ¹⁸F, ³⁵S, ³⁶Cl, ⁸²Br, ⁷⁵Br,⁷⁶Br, ⁷⁷Br, ¹²³I, ¹²⁴I, ¹²⁵I and ¹³¹I. For example, one or more hydrogenatoms in a compound of the present disclosure can be replaced bydeuterium atoms (e.g., one or more hydrogen atoms of a C₁₋₆ alkyl groupof Formula (I) can be optionally substituted with deuterium atoms, suchas —CD₃ being substituted for —CH₃). In some embodiments, alkyl groupsof the disclosed Formulas (e.g., Formula (I)) can be perdeuterated.

One or more constituent atoms of the compounds presented herein can bereplaced or substituted with isotopes of the atoms in natural ornon-natural abundance. In some embodiments, the compound includes atleast one deuterium atom. For example, one or more hydrogen atoms in acompound presented herein can be replaced or substituted by deuterium(e.g., one or more hydrogen atoms of a C₁₋₆ alkyl group can be replacedby deuterium atoms, such as —CD₃ being substituted for —CH₃). In someembodiments, the compound includes two or more deuterium atoms. In someembodiments, the compound includes 1-2, 1-3, 1-4, 1-5, or 1-6 deuteriumatoms. In some embodiments, all of the hydrogen atoms in a compound canbe replaced or substituted by deuterium atoms.

In some embodiments, 1, 2, 3, 4, 5, 6, 7, or 8 hydrogen atoms, attachedto carbon atoms of alkyl, alkenyl, alkynyl, aryl, phenyl, cycloalkyl,heterocycloalkyl, or heteroaryl substituents or —C₁₋₄ alkyl-, alkylene,alkenylene and alkynylene linking groups, as described herein, areoptionally replaced by deuterium atoms.

Synthetic methods for including isotopes into organic compounds areknown in the art (Deuterium Labeling in Organic Chemistry by Alan F.Thomas (New York, N.Y., Appleton-Century-Crofts, 1971; The Renaissanceof H/D Exchange by Jens Atzrodt, Volker Derdau, Thorsten Fey and JochenZimmermann, Angew. Chem. Int. Ed. 2007, 7744-7765; The Organic Chemistryof Isotopic Labelling by James R. Hanson, Royal Society of Chemistry,2011). Isotopically labeled compounds can be used in various studiessuch as NMR spectroscopy, metabolism experiments, and/or assays.

Substitution with heavier isotopes, such as deuterium, may affordcertain therapeutic advantages resulting from greater metabolicstability, for example, increased in vivo half-life or reduced dosagerequirements, and hence may be preferred in some circumstances. (seee.g., A. Kerekes et al. J. Med. Chem. 2011, 54, 201-210; R. Xu et al. J.Label Compd. Radiopharm. 2015, 58, 308-312). In particular, substitutionat one or more metabolism sites may afford one or more of thetherapeutic advantages.

The radionuclide that is incorporated in the instant radio-labeledcompounds will depend on the specific application of that radio-labeledcompound. For example, for in vitro CDK2 labeling and competitionassays, compounds that incorporate ³H, ¹⁴C, ⁸²Br, ¹²⁵I, ¹³¹I, or ³⁵S canbe useful. For radio-imaging applications ¹¹C, ¹⁸F, ¹²⁵I, ¹²³I, ¹²⁴I,¹³¹I, ⁷⁵Br, ⁷⁶Br, or ⁷⁷Br can be useful.

It is understood that a “radio-labeled” or “labeled compound” is acompound that has incorporated at least one radionuclide. In someembodiments, the radionuclide is selected from the group consisting of³H, ¹⁴C, ¹²⁵I, ³⁵S, and ⁸²Br.

The present disclosure can further include synthetic methods forincorporating radio-isotopes into compounds of the disclosure. Syntheticmethods for incorporating radio-isotopes into organic compounds are wellknown in the art, and an ordinary skill in the art will readilyrecognize the methods applicable for the compounds of disclosure.

A labeled compound of the disclosure can be used in a screening assay toidentify/evaluate compounds. For example, a newly synthesized oridentified compound (i.e., test compound) which is labeled can beevaluated for its ability to bind activate CDK2 by monitoring itsconcentration variation when contacting with CDK2, through tracking ofthe labeling. For example, a test compound (labeled) can be evaluatedfor its ability to reduce binding of another compound which is known toinhibit CDK2 (i.e., standard compound). Accordingly, the ability of atest compound to compete with the standard compound for binding to CDK2directly correlates to its binding affinity. Conversely, in some otherscreening assays, the standard compound is labeled and test compoundsare unlabeled. Accordingly, the concentration of the labeled standardcompound is monitored in order to evaluate the competition between thestandard compound and the test compound, and the relative bindingaffinity of the test compound is thus ascertained.

Kits

The present disclosure also includes pharmaceutical kits useful, forexample, in the treatment or prevention of CDK2-associated diseases ordisorders (such as, e.g., cancer, an inflammatory disease, acardiovascular disease, or a neurodegenerative disease) which includeone or more containers containing a pharmaceutical compositioncomprising a therapeutically effective amount of a compound of thedisclosure. Such kits can further include, if desired, one or more ofvarious conventional pharmaceutical kit components, such as, forexample, containers with one or more pharmaceutically acceptablecarriers, additional containers, etc., as will be readily apparent tothose skilled in the art. Instructions, either as inserts or as labels,indicating quantities of the components to be administered, guidelinesfor administration, and/or guidelines for mixing the components, canalso be included in the kit.

Biomarkers and Pharmacodynamics Markers

The disclosure further provides predictive markers (e.g., biomarkers andpharmacodynamic markers, e.g., gene copy number, gene sequence,expression levels, or phosphorylation levels) to identify those humansubjects having, suspected of having, or at risk of developing a diseaseor disorder associated with CDK2 for whom administering a CDK2 inhibitor(“a CDK2 inhibitor” as used herein refers to a compound of thedisclosure, or a pharmaceutically acceptable salt thereof) is likely tobe effective. The disclosure also provides pharmacodynamic markers(e.g., phosphorylation levels) to identify those human subjects having,suspected of having, or at risk of developing a disease or disorderassociated with CDK2 whom are responding to a CDK2 inhibitor.

The methods are based, at least in part, on the discovery that thefunctional status of cyclin dependent kinase inhibitor 2A (“CDKN2A”;also referred to as “p16”) is a biomarker for predicting sensitivity toCDK2-targeting therapies in G1/S-specific cyclin-E1- (“CCNE1-”)amplified cells suitable for use in patient stratification. In addition,the present invention is based, at least in part, on the discovery that,in CCNE1-amplified cell lines, the level of human retinoblastomaassociated protein (“Rb”) phosphorylation at the serine corresponding toamino acid position 780 of SEQ ID NO:3 is a pharmacodynamic marker forCDK2 activity and is suitable for use in measuring CDK2 enzymaticactivity in cellular assay or preclinical and clinical applications,such as, e.g., monitoring the progress of or responsiveness to treatmentwith a CDK2 inhibitor.

CCNE1 and p16

CCNE1 and p16 have been identified in the Examples as genes, incombination, useful in predicting responsiveness (e.g., improvement indisease as evidenced by disease remission/resolution) of a subjecthaving a disease or disorder associated with CDK2 to a CDK2 inhibitor.

p16 (also known as cyclin-dependent kinase inhibitor 2A,cyclin-dependent kinase 4 inhibitor A, multiple tumor suppressor 1, andp16-INK4a) acts as a negative regulator of the proliferation of normalcells by interacting with CDK4 and CDK6. p16 is encoded by the cyclindependent kinase inhibitor 2A (“CDKN2A”) gene (GenBank Accession No.NM_000077). The cytogenic location of the CDKN2A gene is 9p21.3, whichis the short (p) arm of chromosome 9 at position 21.3. The molecularlocation of the CDKN2A gene is base pairs 21,967,752 to 21,995,043 onchromosome 9 (Homo sapiens Annotation Release 109, GRCh38.p12). Geneticand epigenetic abnormalities in the gene encoding p16 are believed tolead to escape from senescence and cancer formation (Okamoto et al.,1994, PNAS 91(23):11045-9). Nonlimiting examples of geneticabnormalities in the gene encoding p16 are described in Table A, below.The amino acid sequence of human p16 is provided below (GenBankAccession No. NP_000068/UniProtKB Accession No. P42771):

(SEQ ID NO: 1) 1 MEPAAGSSME PSADWLATAA ARGRVEEVRA LLEAGALPNAPNSYGRRPIQ VMMMGSARVA 61 ELLLLHGAEP NCADPATLTR PVHDAAREGF LDTLVVLHRAGARLDVRDAW GRLPVDLAEE 121 LGHRDVARYL RAAAGGTRGS NHARIDAAEG PSDIPD 

CCNE1 is a cell cycle factor essential for the control of the cell cycleat the G1/S transition (Ohtsubo et al., 1995, Mol. Cell. Biol.15:2612-2624). CCNE1 acts as a regulatory subunit of CDK2, interactingwith CDK2 to form a serine/threonine kinase holoenzyme complex. TheCCNE1 subunit of this holoenzyme complex provides the substratespecificity of the complex (Honda et al., 2005, EMBO 24:452-463). CCNE1is encoded by the cyclin E1 (“CCNE1”) gene (GenBank Accession No.NM_001238). The amino acid sequence of human CCNE1 is provided below(GenBank Accession No. NP_001229/UniProtKB Accession No. P24864):

(SEQ ID NO: 2) 1 mprerrerda kerdtmkedg gaefsarsrk rkanvtvflqdpdeemakid rtardqcgsq 61 pwdnnavcad pcsliptpdk edddrvypns tckpriiapsrgsplpvlsw anreevwkim 121 lnkektylrd qhfleghpll qpkmrailld wlmevcevyklhretfylaq dffdrymatq 181 envvktllql igisslfiaa kleeiyppkl hqfayvtdgacsgdeiltme lmimkalkwr 241 lspltivswl nvymqvayln dlhevllpqy pqqifiqiaelldlcvldvd clefpygila 301 asalyhfsss elmqkvsgyq wcdiencvkw mvpfamviretgssklkhfr gvadedahni 361 qthrdsldll dkarakkaml seqnrasplp sglltppqsgkkqssgpema.

The Examples demonstrate CDK2-knockdown inhibits proliferation ofCCNE1-amplified cell lines, but not of CCNE-non-amplified cell lines.Conversely, the Examples show that CDK4/6 inhibition inhibitsproliferation of CCNE1-non-amplified cell lines, but not ofCCNE-amplified cell lines. The Examples further demonstrate thatpresence of a normal (e.g., non-mutated or non-deleted) p16 gene isrequired for the observed inhibition of cell proliferation inCCNE1-amplified cells treated with a CDK2-inhibitor. Accordingly, CCNE1and p16 are, together, a combination biomarker: cells that respond totreatment with a CDK2 inhibitor display an amplification of the CCNE1gene and/or an expression level of CCNE1 that is higher than a controlexpression level of CCNE1, and have a nucleotide sequence (e.g., a geneor an mRNA) that encodes the p16 protein (e.g., a p16 protein comprisingthe amino acid sequence of SEQ ID NO:1) and/or have p16 protein present,while control cells that do not respond to treatment with a CDK2inhibitor do not have an amplification of the CCNE1 gene and/or anexpression level of CCNE1 that is higher than a control expression levelof CCNE1, and tend to have a mutated or deleted gene that encodes thep16 protein and/or lack expression of p16 protein.

Thus, the disclosure provides a method of treating a human subjecthaving, suspected of having, or at risk of developing a disease ordisorder associated with CDK2, comprising administering to the humansubject a CDK2 inhibitor, wherein the human subject has been previouslydetermined to: (i) (a) have a nucleotide sequence encoding a p16 proteincomprising the amino acid sequence of SEQ ID NO:1, (b) have a CDKN2Agene lacking one or more inactivating nucleic acid substitutions and/ordeletions, and/or (c) express a p16 protein, and (ii) (a) have anamplification of the CCNE1 gene and/or (b) have an expression level ofCCNE1 in a biological sample obtained from the human subject that ishigher than a control expression level of CCNE1. In certain embodiments,the predictive methods described herein predict that the subject willrespond to treatment with the CDK2 inhibitor with at least 50%, at least60%, at least 70%, at least 80%, at least 90%, at least 95%, at least98% or 100% accuracy. For example, in some embodiments, if thepredictive methods described herein are applied to 10 subjects having,suspected of having, or at risk of developing a disease or disorderassociated with CDK2, and 8 of those 10 subjects are predicted torespond to treatment with a CDK2 inhibitor based on a predictive methoddescribed herein, and 7 of those 8 subjects do indeed respond totreatment with a CDK2 inhibitor, then the predictive method has anaccuracy of 87.5% (7 divided by 8). A subject is considered to respondto the CDK2 inhibitor if the subject shows any improvement in diseasestatus as evidenced by, e.g., reduction or alleviation in symptoms,disease remission/resolution, etc.

In some embodiments, the subject has a disease or disorder associatedwith CDK2. In some embodiments, the human subject has been previouslydetermined to: (i) (a) have a nucleotide sequence encoding a p16 proteincomprising the amino acid sequence of SEQ ID NO:1 and/or (b) a CDKN2Agene lacking one or more inactivating nucleic acid substitutions and/ordeletions, and (ii) have an amplification of the CCNE1 gene in abiological sample obtained from the human subject. In some embodiments,the CDKN2A gene encodes a protein comprising the amino acid sequence ofSEQ ID NO:1. In specific embodiments, the CDKN2A gene encodes a proteincomprising the amino acid sequence of SEQ ID NO:1.

In specific embodiments, the one or more inactivating nucleic acidsubstitutions and/or deletions in the CDKN2A gene is as described inTable A. In specific embodiments, the one or more inactivating nucleicacid substitutions and/or deletions in the CDKN2A gene is as describedin Yarbrough et al., Journal of the National Cancer Institute,91(18):1569-1574, 1999; Liggett and Sidransky, Biology of Neoplasia,Journal of Oncology, 16(3):1197-1206, 1998, and Cairns et al., NatureGenetics, 11:210-212, 1995, each of which is incorporated by referenceherein in its entirety.

TABLE A CDKN2A gene substitutions, deletions, and modificationsDescription Reference(s) C to T transition converting codon 232 of theRefSNP Accession No. CDKN2A gene from an arginine codon to ars121913388; Kamb et al., Science stop codon 264: 436-440, 199419-basepair germline deletion at nucleotide 225 RefSNP Accession No.causing a reading-frame shift predicted to rs587776716; Gruis et al.,Nature severely truncate p16 protein Genet. 10: 351-353, 1995 6-basepairdeletion at nucleotides 363-368 of ClinVar Accession No. the CDKN2A geneRCV000010017.2; Liu et al., Oncogene 11: 405-412, 1995 Mutation atchromosome 9:21971058 predicted RefSNP Accession No. to substituteglycine corresponding to amino rs104894094; Ciotti et al., Am. J. acidposition 101 of SEQ ID NO: 1 with a Hum. Genet. 67: 311-319, 2000tryptophan Germline mutation constituting an in-frame 3- ClinVarAccession No. basepair duplication at nucleotide 332 in exon 2RCV000010020.3; Borg et al., of the CDKN2A gene Cancer Res. 56:2497-2500, 1996 Mutation predicted to substitute methionine RefSNPAccession No. corresponding to amino acid position 53 of rs104894095;Harland et al., Hum. SEQ ID NO: 1 with an isoleucine Molec. Genet. 6:2061-2067, 1997 Mutation predicted to substitute arginine RefSNPAccession No. corresponding to amino acid position 24 of rs104894097;Monzon et al., New SEQ ID NO: 1 with a proline Eng. J. Med. 338:879-887, 1998 24-basepair repeat inserted at chromosome 9 RefSNPAccession No. between 21974795 and 21974796 (forward rs587780668;Pollock et al., Hum. strand) Mutat. 11: 424-431, 1998) G-to-Ttransversion at nucleotide −34 of the ClinVar Accession No. CDKN2A geneRCV000010024.5; Liu et al., Nature Genet. 21: 128-132, 1999 Deletion ofthe p14(ARF)-specific exon 1-beta ClinVar Accession No. of CDKN2ARCV000010026.2; Randerson-Moor et al., Hum. Molec. Genet. 10: 55- 62,2001 Mutation predicted to substitute valine RefSNP Accession No.corresponding to amino acid position 126 of rs104894098; Goldstein etal., Brit. SEQ ID NO: 1 with an isoleucine J. Cancer 85: 527-530, 2001Transition (IVS2-105 A-G) in intron 2 of the ClinVar Accession No.CDKN2A gene creating a false GT splice donor RCV000010028.3; Harland etal., site 105 bases 5-prime of exon 3 resulting in Hum. Molec. Genet.10: 2679-2686, aberrant splicing of the mRNA 2001 Mutation predicted toresult in substitution of RefSNP Accession No. glycine corresponding toamino acid position rs113798404; Hewitt et al., Hum. 122 of SEQ ID NO: 1with an arginine Molec. Genet. 11: 1273-1279, 2002 Mutation predicted toresult in substitution of RefSNP Accession No. valine corresponding toamino acid position 59 rs113798404; Yakobson et al., of SEQ ID NO: 1with an arginine Melanoma Res. 11: 569-570, 2001 Tandem germline339G-Ctransversion and a RefSNP Accession Nos. 340C-T transition in the CDKN2Agene rsl 13798404 and rs104894104; resulting in substitution of prolineKannengiesser et al., Genes corresponding to amino acid position 114 ofChromosomes Cancer 46: 751-760, SEQ ID NO: 1 with a serine 2007 Mutationpredicted to result in substitution of RefSNP Accession No. serinecorresponding to amino acid position 56 rs104894109; Kannengiesser etal., of SEQ ID NO: 1 with an isoleucine Genes Chromosomes Cancer 46:751-760, 2007 Mutation predicted to result in substitution of RefSNPAccession No. glycine corresponding to amino acid position rs137854599;Goldstein et al., J. 89 of SEQ ID NO: 1 with an aspartic acid Med.Genet. 45: 284-289, 2008 Heterozygous A-to-G transition in exon 1B ofClinVar Accession no. the CDKN2A gene, affecting splicing of theRCV000022943.3; Binni et al., Clin. p14(ARF) isoform Genet. 77: 581-586,2010 Heterozygous 5-bp duplication (19_23dup) in ClinVar Accession No.the CDKN2A gene, resulting in a frameshift RCV000030680.6; Harinck, F.,and premature termination Kluijt et al., J. Med. Genet. 49: 362- 365,2012 Mutation predicted to result in substitution of Yarbrough et al.,Journal of the aspartic acid corresponding to amino acid National CancerInstitute, position 84 of SEQ ID NO: 1 with a valine 91(18): 1569-1574Mutation predicted to result in substitution of Yarbrough et al.,Journal of the aspartic acid corresponding to amino acid National CancerInstitute, position 84 of SEQ ID NO: 1 with a glycine 91(18): 1569-1574Mutation predicted to result in substitution of Yarbrough et al.,Journal of the arginine corresponding to amino acid position NationalCancer Institute, 87 of SEQ ID NO: 1 with a proline 91(18): 1569-1574Mutation predicted to result in substitution of Yarbrough et al.,Journal of the proline corresponding to amino acid position 48 NationalCancer Institute, of SEQ ID NO: 1 with a leucine 91(18): 1569-1574Mutation predicted to result in substitution of Yarbrough et al.,Journal of the aspartic acid corresponding to amino acid National CancerInstitute, position 74 of SEQ ID NO: 1 with a asparagine 91(18):1569-1574 Mutation predicted to result in substitution of Yarbrough etal., Journal of the arginine corresponding to amino acid positionNational Cancer Institute, 87 of SEQ ID NO: 1 with a leucine 91(18):1569-1574 Mutation predicted to result in substitution of Yarbrough etal., Journal of the asparagine corresponding to amino acid NationalCancer Institute, position 71 of SEQ ID NO: 1 with a serine 91(18):1569-1574 Mutation predicted to result in substitution of Yarbrough etal., Journal of the arginine corresponding to amino acid positionNational Cancer Institute, 80 of SEQ ID NO: 1 with a leucine 91(18):1569-1574 Mutation predicted to result in substitution of Yarbrough etal., Journal of the histidine corresponding to amino acid positionNational Cancer Institute, 83 of SEQ ID NO: 1 with a tyrosine 91(18):1569-1574

The disclosure also features a method of treating a human subjecthaving, suspected of having, or at risk of developing a disease ordisorder associated with CDK2, comprising: (i) identifying, in abiological sample obtained from the human subject: (a) a nucleotidesequence encoding a p16 protein comprising the amino acid sequence ofSEQ ID NO:1, (b) a CDKN2A gene lacking one or more inactivating nucleicacid substitutions, and/or (c) the presence of a p16 protein; (ii)identifying, in a biological sample obtained from the human subject: (a)an amplification of the CCNE1 gene and/or (b) an expression level ofCCNE1 that is higher than a control expression level of CCNE1; and (iii)administering a CDK2 inhibitor to the human subject. In someembodiments, the subject has a disease or disorder associated with CDK2.In some embodiments, the subject is suspected of having or is at risk ofdeveloping a disease or disorder associated with CDK2. In someembodiments, the method comprises: (i) identifying, in a biologicalsample obtained from the human subject: (a) a nucleotide sequenceencoding a p16 protein comprising the amino acid sequence of SEQ IDNO:1, (b) a CDKN2A gene lacking one or more inactivating nucleic acidsubstitutions and/or deletions, and/or (c) the presence of a p16protein; (ii) identifying, in a biological sample obtained from thehuman subject: (a) an amplification of the CCNE1 gene; and (iii)administering a CDK2 inhibitor to the human subject.

The disclosure also features a method of predicting the response of ahuman subject having, suspected of having, or at risk of developing adisease or disorder associated with CDK2 to a CDK2 inhibitor,comprising: (i) determining, from a biological sample obtained from thehuman subject: (a) the nucleotide sequence of a CDKN2A gene, (b) thepresence of a CDKN2A gene lacking one or more inactivating nucleic acidsubstitutions and/or deletions, and/or (c) the presence of a p16protein; and (ii) determining, from a biological sample obtained fromthe human subject: (a) the copy number of the CCNE1 gene and/or (b) theexpression level of CCNE1, wherein (1) (a) the presence of a CDKN2A geneencoding a p16 protein comprising the amino acid sequence of SEQ IDNO:1, (b) the presence of a CDKN2A gene lacking one or more inactivatingnucleic acid substitutions and/or deletions, and/or (c) the presence ofa p16 protein, and (2) (a) an amplification of the CCNE1 gene and/or (b)an expression level of CCNE1 that is higher than a control expressionlevel of CCNE1, is predictive that the human subject will respond to theCDK2 inhibitor. In some embodiments, the subject has a disease ordisorder associated with CDK2. In some embodiments, the subject issuspected of having or is at risk of developing a disease or disorderassociated with CDK2. In some embodiments, the method comprises: (i)determining, from a biological sample obtained from the human subject:(a) the nucleotide sequence of a CDKN2A gene and/or (b) the presence ofa CDKN2A gene lacking one or more inactivating nucleic acidsubstitutions and/or deletions; and (ii) determining, from a biologicalsample obtained from the human subject: (a) the copy number of the CCNE1gene, wherein (1) (a) the presence of a CDKN2A gene encoding a p16protein comprising the amino acid sequence of SEQ ID NO:1 and/or (b) thepresence of a CDKN2A gene lacking one or more inactivating nucleic acidsubstitutions and/or deletions, and (2) (a) an amplification of theCCNE1 gene, is predictive that the human subject will respond to theCDK2 inhibitor.

In specific embodiments, the (i) determining of (a) the nucleotidesequence of a CDKN2A gene, (b) the presence of a CDKN2A gene lacking oneor more inactivating nucleic acid substitutions and/or deletions, and/or(c) the presence of a p16 protein is performed before (e.g., at least 1day, at least 2 days, at least 3 days, at least 4 days, at least 5 days,at least 6 days, at least 7 days, at least 2 weeks, at least 3 weeks, orat least 4 weeks, or from 6 hours to 16 hours, from 6 hours to 20 hours,or from 6 hours to 24 hours, from 2 days to 3 days, from 2 days to 4days, from 2 days to 5 days, from 2 days to 6 days, from 2 days to 7days, from 1 week to 2 weeks, from 1 week to 3 weeks, or from 1 week to4 weeks before) administering to the human subject the CDK2 inhibitor.In specific embodiments, the (ii) determining of (a) the copy number ofthe CCNE1 gene and/or (b) the expression level of CCNE1 in thebiological sample obtained from the human subject is performed before(e.g., at least 1 day, at least 2 days, at least 3 days, at least 4days, at least 5 days, at least 6 days, at least 7 days, at least 2weeks, at least 3 weeks, or at least 4 weeks, or from 6 hours to 16hours, from 6 hours to 20 hours, or from 6 hours to 24 hours, from 2days to 3 days, from 2 days to 4 days, from 2 days to 5 days, from 2days to 6 days, from 2 days to 7 days, from 1 week to 2 weeks, from 1week to 3 weeks, or from 1 week to 4 weeks before) administering to thehuman subject the CDK2 inhibitor.

An amplification of the CCNE1 gene and/or an expression level of CCNE1that is higher than a control expression level of CCNE1, combined withthe presence of a CDKN2A gene encoding a p16 protein comprising theamino acid sequence of SEQ ID NO:1, the presence of a CDKN2A genelacking one or more inactivating nucleic acid substitutions and/ordeletions, and/or the presence of a p16 protein (e.g., a p16 proteincomprising the amino acid sequence of SEQ ID NO:1), isindicative/predictive that a human subject having, suspected of having,or at risk of developing a disease or disorder associated with CDK2 willrespond to a CDK2 inhibitor.

In some embodiments, the CCNE1 gene is amplified to a gene copy numberfrom 3 to 25. In specific embodiments, the CCNE1 gene is amplified to agene copy number of at least 3. In specific embodiments, the CCNE1 geneis amplified to a gene copy number of at least 5. In specificembodiments, the CCNE1 gene is amplified to a gene copy number of atleast 7. In specific embodiments, the CCNE1 gene is amplified to a genecopy number of at least 10. In specific embodiments, the CCNE1 gene isamplified to a gene copy number of at least 12. In specific embodiments,the CCNE1 gene is amplified to a gene copy number of at least 14. Inspecific embodiments, the CCNE1 gene is amplified to a gene copy numberof at least 21.

In specific embodiments, the expression level of CCNE1 is the level ofCCNE1 mRNA. In specific embodiments, the expression level of CCNE1 isthe level of CCNE1 protein.

In some embodiments of the foregoing methods, the control expressionlevel of CCNE1 is a pre-established cut-off value. In some embodimentsof the foregoing methods, the control expression level of CCNE1 is theexpression level of CCNE1 in a sample or samples obtained from one ormore subjects that have not responded to treatment with the CDK2inhibitor.

In some embodiments of the foregoing methods, the expression level ofCCNE1 is the expression level of CCNE1 mRNA. In some embodiments of theforegoing methods, the expression level of CCNE1 is the expression levelof CCNE1 protein. In some embodiments in which the expression level ofCCNE1 is the expression level of CCNE1 mRNA, the expression level ofCCNE1 is measured by RNA sequencing, quantitative polymerase chainreaction (PCR), in situ hybridization, nucleic acid array or RNAsequencing. In some embodiments in which the expression level of CCNE1is the expression level of CCNE1 protein, the expression level of CCNE1is measured by western blot, enzyme-linked immunosorbent assay, orimmunohistochemistry staining.

Rb S780

The disclosure also features a method for assessing the CDKN2A gene andthe CCNE1 gene, comprising determining, from a biological sample orbiological samples obtained from a human subject having a disease ordisorder associated with CDK2, (i) (a) the nucleotide sequence of aCDKN2A gene or (b) the presence of a CDKN2A gene lacking one or moreinactivating nucleic acid substitutions and/or deletions, and (ii) thecopy number of the CCNE1 gene.

The disclosure also features a method of evaluating the response of ahuman subject having, suspected of having, or at risk of developing adisease or disorder associated with CDK2 to a CDK2 inhibitor,comprising: (a) administering a CDK2 inhibitor to the human subject,wherein the human subject has been previously determined to have anamplification of the CCNE1 gene and/or an expression level of CCNE1 thatis higher than a control expression level of CCNE1; (b) measuring, in abiological sample of obtained from the subject subsequent to theadministering of step (a), the level of retinoblastoma (Rb) proteinphosphorylation at the serine corresponding to amino acid position 780of SEQ ID NO:3, wherein a reduced level of Rb phosphorylation at theserine corresponding to amino acid position 780 of SEQ ID NO:3, ascompared to a control level of Rb phosphorylation at the serinecorresponding to amino acid position 780 of SEQ ID NO:3, is indicativethat the human subject responds to the CDK2 inhibitor. In someembodiments, the subject has a disease or disorder associated with CDK2.In some embodiments, the subject is suspected of having or is at risk ofdeveloping a disease or disorder associated with CDK2. In someembodiments, the biological sample comprises a blood sample or a tumorbiopsy sample.

Phosphorylation of Rb at the serine corresponding to amino acid position780 of SEQ ID NO:3 (referred to herein as “Ser780” or “S780”) has beenidentified in the Examples as a pharmacodynamic marker useful inassessing responsiveness (e.g., inhibition by CDK2) of a human subjecthaving a disease or disorder having CCNE1 amplification to a CDK2inhibitor.

Rb is a regulator of the cell cycle and acts as a tumor suppressor. Rbis activated upon phosphorylation by cyclin D-CDK4/6 at Ser780 andSer795 and by cyclin E/CDK2 at Ser807 and Ser811. Rb is encoded by theRB transcriptional corepressor I (“RB”) gene (GenBank Accession No.NM_000321). The amino acid sequence of human Rb is provided below(GenBank Accession No. NP_000312/UniProtKB Accession No. P06400) (S780is in bold and underlined):

(SEQ ID NO: 3) 1 MPPKTPRKTA ATAAAAAAEP PAPPPPPPPE EDPEQDSGPEDLPLVRLEFE ETEEPDFTAL 61 CQKLKIPDHV RERAWLTWEK VSSVDGVLGG YIQKKKELWGICIFIAAVDL DEMSFTFTEL 121 QKNIEISVHK FFNLLKEIDT STKVDNAMSR LLKKYDVLFALFSKLERTCE LIYLTQPSSS 181 ISTEINSALV LKVSWITFLL AKGEVLQMED DLVISFQLMLCVLDYFIKLS PPMLLKEPYK 241 TAVIPINGSP RTPRRGQNRS ARIAKQLEND TRIIEVLCKEHECNIDEVKN VYFKNFIPFM 301 NSLGLVTSNG LPEVENLSKR YEEIYLKNKD LDARLFLDHDKTLQTDSIDS FETQRTPRKS 361 NLDEEVNVIP PHTPVRTVMN TIQQLMMILN SASDQPSENLISYFNNCTVN PKESILKRVK 421 DIGYIFKEKF AKAVGQGCVE IGSQRYKLGV RLYYRVMESMLKSEEERLSI QNFSKLLNDN 481 IFHMSLLACA LEVVMATYSR STSQNLDSGT DLSFPWILNVLNLKAFDFYK VIESFIKAEG 541 NLTREMIKHL ERCEHRIMES LAWLSDSPLF DLIKQSKDREGPTDHLESAC PLNLPLQNNH 601 TAADMYLSPV RSPKKKGSTT RVNSTANAET QATSAFQTQKPLKSTSLSLF YKKVYRLAYL 661 RLNTLCERLL SEHPELEHII WTLFQHTLQN EYELMRDRHLDQIMMCSMYG ICKVKNIDLK 721 FKIIVTAYKD LPHAVQETFK RVLIKEEEYD SIIVFYNSVFMQRLKTNILQ YASTRPPTL S 781 PIPHIPRSPY KFPSSPLRIP GGNIYISPLK SPYKISEGLPTPTKMTPRSR ILVSIGESFG 841 TSEKFQKINQ MVCNSDRVLK RSAEGSNPPK PLKKLRFDIEGSDEADGSKH LPGESKFQQK 901 LAEMTSTRTR MQKQKMNDSM DTSNKEEK

As stated above, the Examples demonstrate CDK2-knockdown inhibitsproliferation in CCNE1-amplified cell lines, but not inCCNE1-non-amplified cell lines. The Examples further demonstrateCDK2-knockdown or inhibition blocks Rb phosphorylation at the S780 inCCNE1-amplified cell lines, but not in CCNE1-non-amplified cell lines.Accordingly, Rb phosphorylation at the serine corresponding to aminoacid position 780 of SEQ ID NO:3 is a pharmacodynamic marker forassessing response to CDK2 inhibition in CCNE1 amplified cancer cells orpatients with diseases or disorders having CCNE1 amplification. Thus,provided herein are methods relating to the use of the level of Rbphosphorylation at the serine corresponding to amino acid position 780of SEQ ID NO:3 in a human subject having, suspected of having, or atrisk of developing a disease or disorder associated with CDK2 as amarker for indicating the response of the human subject to a CDK2inhibitor, wherein the human subject has an increased expression levelof CCNE1.

Thus, the disclosure features a method for measuring the amount of aprotein in a sample, comprising: (a) providing a biological sampleobtained from a human subject having a disease or disorder associatedwith CDK2; and (b) measuring the level of Rb protein phosphorylation atthe serine corresponding to amino acid position 780 of SEQ ID NO:3 inthe biological sample. In some embodiments, the biological samplecomprises a blood sample or a tumor biopsy sample. In a specificembodiment, provided herein is a method of evaluating the response of ahuman subject having, suspected of having, or at risk of developing adisease or disorder associated with CDK2 to a CDK2 inhibitor,comprising: (a) administering a CDK2 inhibitor to the human subject,wherein the human subject has been previously determined to have anamplification of the CCNE1 gene and/or an expression level of CCNE1 thatis higher than a control expression level of CCNE1; and (b) measuring,in a biological sample obtained from the human subject subsequent to theadministering of step (a), the level of Rb phosphorylation at the serinecorresponding to amino acid position 780 of SEQ ID NO:3, wherein areduced level of Rb phosphorylation at the serine corresponding to aminoacid position 780 of SEQ ID NO:3, as compared to a control level of Rbphosphorylation at the serine corresponding to amino acid position 780of SEQ ID NO:3, is indicative that the human subject responds to theCDK2 inhibitor. In specific embodiments, the human subject has a diseaseor disorder associated with CDK2.

A reduced level of Rb phosphorylation at the serine corresponding toamino acid position 780 of SEQ ID NO:3, as compared to a control levelof Rb phosphorylation at the serine corresponding to amino acid position780 of SEQ ID NO:3, combined with an amplification of the CCNE1 geneand/or an expression level of CCNE1 that is higher than a controlexpression level of CCNE1, is indicative that a human subject having,suspected of having, or at risk of developing a disease or disorderassociated with CDK2 responds to a CDK2 inhibitor. For example, in asubject having an amplification of the CCNE1 gene and/or an expressionlevel of CCNE1 that is higher than a control expression level of CCNE1,a biological sample, obtained from the subject after treatment with aCDK2 inhibitor, having low (e.g., reduced as compared to a control) orundetectable levels of Rb phosphorylation at serine corresponding toamino acid position 780 of SEQ ID NO:3 is indicative that the subjectresponds to the CDK2 inhibitor.

A biological sample, obtained from a subject after administration of aCDK2 inhibitor to the subject, having a reduced level of Rbphosphorylation at the serine corresponding to amino acid position 780of SEQ ID NO:3, as compared to a control level of Rb phosphorylation atthe serine corresponding to amino acid position 780 of SEQ ID NO:3,combined with: (i) an amplification of the CCNE1 gene and/or anexpression level of CCNE1 that is higher than a control expression levelof CCNE1, and (ii) presence of a CDKN2A gene encoding a p16 proteincomprising the amino acid sequence of SEQ ID NO:1, presence of a CDKN2Agene lacking one or more inactivating nucleic acid substitutions and/ordeletions, and/or presence of a p16 protein (e.g., a p16 proteincomprising the amino acid sequence of SEQ ID NO:1), is indicative that ahuman subject having, suspected of having, or at risk of developing adisease or disorder associated with CDK2 responds to a CDK2 inhibitor.For example, in a human subject having (i) an amplification of the CCNE1gene and/or an expression level of CCNE1 that is higher than a controlexpression level of CCNE1, and (ii) the presence of a CDKN2A geneencoding a p16 protein comprising the amino acid sequence of SEQ IDNO:1, the presence of a CDKN2A gene lacking one or more inactivatingnucleic acid substitutions and/or deletions, and/or the presence of ap16 protein (e.g., a p16 protein comprising the amino acid sequence ofSEQ ID NO:1), a biological sample, obtained from the human subject afteradministration of a CDK2 inhibitor to the subject, having low (e.g.,reduced as compared to a control) or undetectable levels of Rbphosphorylation at the serine corresponding to amino acid position 780of SEQ ID NO:3 is indicative that the human subject responds to the CDK2inhibitor

In some embodiments, the CCNE1 gene is amplified to a gene copy numberfrom 3 to 25. In specific embodiments, the CCNE1 gene is amplified to agene copy number of at least 3. In specific embodiments, the CCNE1 geneis amplified to a gene copy number of at least 5. In specificembodiments, the CCNE1 gene is amplified to a gene copy number of atleast 7. In specific embodiments, the CCNE1 gene is amplified to a genecopy number of at least 10. In specific embodiments, the CCNE1 gene isamplified to a gene copy number of at least 12. In specific embodiments,the CCNE1 gene is amplified to a gene copy number of at least 14. Inspecific embodiments, the CCNE1 gene is amplified to a gene copy numberof at least 21. In specific embodiments, the expression level of CCNE1is the level of CCNE1 mRNA. In specific embodiments, the expressionlevel of CCNE1 is the level of CCNE1 protein.

Controls

As described above, the methods related to biomarkers andpharmacodynamic markers can involve, measuring one or more markers(e.g., a biomarker or a pharmacodynamics marker, e.g., the amplificationof the CCNE1 gene, the expression level of CCNE1, the presence of aCDKN2A gene encoding a p16 protein comprising the amino acid sequence ofSEQ ID NO:1, the presence of a CDKN2A gene lacking one or moreinactivating nucleic acid substitutions and/or deletions, the presenceof a p16 protein (e.g., a p16 protein comprising the amino acid sequenceof SEQ ID NO:1), and Rb phosphorylation at the serine corresponding toamino acid position 780 of SEQ ID NO:3) in a biological sample from ahuman subject having, suspected of having or at risk of developing adisease or disorder associated with CDK2. In specific embodiments, thehuman subject has a disease or disorder associated with CDK2. Inspecific embodiments, the human subject is suspected of having or is atrisk of developing a disease or disorder associated with CDK2. Incertain aspects, the level (e.g., amplification (e.g., for the CCNE1gene), expression level (e.g., for CCNE1 or p16 protein), orphosphorylation level (e.g., for Rb)) of one or more biomarkers,compared to a control level of the one or more biomarkers,predicts/indicates the response of a human subject to treatmentcomprising a CDK2 inhibitor. In certain embodiments, when (i) the CCNE1gene is amplified and/or an expression level of CCNE1 that is higherthan a control expression level of CCNE1, and (ii) a CDKN2A geneencoding a p16 protein comprising the amino acid sequence of SEQ ID NO:1is present, a CDKN2A gene lacking one or more inactivating nucleic acidsubstitutions and/or deletions is present, and/or a p16 protein (e.g., ap16 protein comprising the amino acid sequence of SEQ ID NO:1) ispresent, the human subject is identified as likely to respond to a CDK2inhibitor. In other embodiments, when (i) the CCNE1 gene is amplifiedand/or an expression level of CCNE1 that is higher than a controlexpression level of CCNE1, and (ii) in a biological sample from thehuman subject after the human subject has been administered a CDK2inhibitor, the level of Rb phosphorylation at the serine correspondingto amino acid position 780 of SEQ ID NO:3 is less than the control levelof Rb phosphorylation at the serine corresponding to amino acid position780 of SEQ ID NO:3, the human subject is identified as responding to aCDK2 inhibitor. In yet another embodiment, when (i) the CCNE1 gene isamplified and/or an expression level of CCNE1 that is higher than acontrol expression level of CCNE1, (ii) a CDKN2A gene encoding a p16protein comprising the amino acid sequence of SEQ ID NO:1 is present, aCDKN2A gene lacking one or more inactivating nucleic acid substitutionsand/or deletions is present, and/or a p16 protein (e.g., a p16 proteincomprising the amino acid sequence of SEQ ID NO:1) is present, and (iii)in a biological sample from the human subject after the human subjecthas been administered a CDK2 inhibitor, the level of Rb phosphorylationat the serine corresponding to amino acid position 780 of SEQ ID NO:3 isless than the control level of Rb phosphorylation at the serinecorresponding to amino acid position 780 of SEQ ID NO:3, the humansubject is identified as responding to a CDK2 inhibitor. In thiscontext, the term “control” includes a sample (from the same tissuetype) obtained from a human subject who is known to not respond to aCDK2 inhibitor. The term “control” also includes a sample (from the sametissue type) obtained in the past from a human subject who is known tonot respond to a CDK2 inhibitor and used as a reference for futurecomparisons to test samples taken from human subjects for whichtherapeutic responsiveness is to be predicted. The “control” level(e.g., gene copy number, expression level, or phosphorylation level) fora particular biomarker (e.g., CCNE1, p16, or Rb phosphorylation) in aparticular cell type or tissue may be pre-established by an analysis ofbiomarker level (e.g., expression level or phosphorylation level) in oneor more (e.g., 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 35, or 40 ormore) human subjects that have not responded to treatment with a CDK2inhibitor. This pre-established reference value (which may be an averageor median level (e.g., gene copy number, expression level, orphosphorylation level) taken from multiple human subjects that have notresponded to the therapy) may then be used for the “control” level ofthe biomarker (e.g., CCNE, p16, or Rb phosphorylation) in the comparisonwith the test sample. In such a comparison, the human subject ispredicted to respond to a CDK2 inhibitor if the CCNE1 gene is amplifiedand/or the expression level of CCNE is higher than the pre-establishedreference, and a CDKN2A gene encoding a p16 protein comprising the aminoacid sequence of SEQ ID NO:1 is present, a CDKN2A gene lacking one ormore inactivating nucleic acid substitutions and/or deletions ispresent, and/or a p16 protein (e.g., a p16 protein comprising the aminoacid sequence of SEQ ID NO:1) is present. In another such a comparison,the human subject is predicted to respond to a CDK2 inhibitor if (i)CCNE1 gene is amplified and/or the expression level of CCNE is higherthan the pre-established reference, and (ii) after administering to thehuman subject a CDK2 inhibitor, the level of Rb phosphorylation at theserine corresponding to amino acid position 780 of SEQ ID NO:3 is lowerthan the pre-established reference. In yet another such a comparison,the human subject is indicated to respond to a CDK2 inhibitor if (i)CCNE1 gene is amplified and/or the expression level of CCNE is higherthan the pre-established reference, (ii) a CDKN2A gene encoding a p16protein comprising the amino acid sequence of SEQ ID NO:1 is present, aCDKN2A gene lacking one or more inactivating nucleic acid substitutionsand/or deletions is present, and/or a p16 protein (e.g., a p16 proteincomprising the amino acid sequence of SEQ ID NO:1) is present, and (iii)after administering to the human subject a CDK2 inhibitor, the level ofRb phosphorylation at the serine corresponding to amino acid position780 of SEQ ID NO:3 is lower than the pre-established reference.

The “control” level for a particular biomarker in a particular cell typeor tissue may alternatively be pre-established by an analysis ofbiomarker level in one or more human subjects that have responded totreatment with a CDK2 inhibitor. This pre-established reference value(which may be an average or median level (e.g., expression level orphosphorylation level) taken from multiple human subjects that haveresponded to the therapy) may then be used as the “control” level (e.g.,expression level or phosphorylation level) in the comparison with thetest sample. In such a comparison, the human subject is indicated torespond to a CDK2 inhibitor if the level (e.g., copy number of the CCNE1gene, expression level of CCNE1, expression level of p16, orphosphorylation level of Rb at the serine corresponding to amino acidposition 780 of SEQ ID NO:3) of the biomarker being analyzed is equal orcomparable to (e.g., at least 85% but less than 115% of), thepre-established reference.

In certain embodiments, the “control” is a pre-established cut-offvalue. A cut-off value is typically a level (e.g., a copy number, anexpression level, or a phosphorylation level) of a biomarker above orbelow which is considered predictive of responsiveness of a humansubject to a therapy of interest. Thus, in accordance with the methodsand compositions described herein, a reference level (e.g., of CCNE1gene copy number, CCNE1 expression, p16 expression, or Rbphosphorylation at the serine corresponding to amino acid position 780of SEQ ID NO:3) is identified as a cut-off value, above or below ofwhich is predictive of responsiveness to a CDK2 inhibitor. Cut-offvalues determined for use in the methods described herein can becompared with, e.g., published ranges of concentrations but can beindividualized to the methodology used and patient population.

In some embodiments, the expression level of CCNE1 is increased ascompared to the expression level of CCNE1 in a control. For example, theexpression level of CCNE1 analyzed can be at least 1.5, at least 2, atleast 3, at least 4, at least 5, at least 6, at least 7, at least 8, atleast 9, at least 10, at least 20, at least 25, at least 50, at least75, or at least 100 times higher, or at least 10%, at least 20%, atleast 30%, at least 40%, at least 50%, at least 60%, at least 70%, atleast 80%, at least 90%, at least 100%, at least 200%, at least 300%, atleast 400%, at least 500%, at least 600%, at least 700%, at least 800%,at least 900%, at least 1,000%, at least 1,500%, at least 2,000%, atleast 2,500%, at least 3,000%, at least 3,500%, at least 4,000%, atleast 4,500%, or at least 5,000% higher, than the expression level ofCCNE1 in a control.

A p16 protein is present if the protein is detectable by any assay knownin the art or described herein, such as, for example, western blot,immunohistochemistry, fluorescence-activated cell sorting, andenzyme-linked immunoassay. In some embodiments, a p16 protein is presentat an expression level that is within at least 5%, at least 10%, atleast 20%, or at least 30% of the p16 expression level in a healthycontrol.

In some embodiments, the level of Rb phosphorylation at the serinecorresponding to amino acid position 780 of SEQ ID NO:3 being analyzedis reduced as compared to the level of Rb phosphorylation at the serinecorresponding to amino acid position 780 of SEQ ID NO:3 in a control.For example, the level of the Rb phosphorylation at the serinecorresponding to amino acid position 780 of SEQ ID NO:3 being analyzedcan be at least 1.5, at least 2, at least 3, at least 4, at least 5, atleast 6, at least 7, at least 8, at least 9, at least 10, at least 20,at least 25, at least 50, at least 75, or at least 100 times lower, orat least 10%, at least 20%, at least 30%, at least 40%, at least 50%, atleast 60%, at least 70%, at least 80%, at least 90%, or 100% lower, thanthe level of Rb phosphorylation at the serine corresponding to aminoacid position 780 of SEQ ID NO:3 in a control.

Biological Samples

Suitable biological samples for the methods described herein include anysample that contains blood or tumor cells obtained or derived from thehuman subject in need of treatment. For example, a biological sample cancontain tumor cells from biopsy from a patient suffering from a solidtumor. A tumor biopsy can be obtained by a variety of means known in theart. Alternatively, a blood sample can be obtained from a patientssuffering from a hematological cancer.

A biological sample can be obtained from a human subject having,suspected of having, or at risk of developing, a disease or disorderassociated with CDK2. In some embodiments, the disease or disorderassociated with CDK2 is a cancer (such as those described supra).

Methods for obtaining and/or storing samples that preserve the activityor integrity of molecules (e.g., nucleic acids or proteins) in thesample are well known to those skilled in the art. For example, abiological sample can be further contacted with one or more additionalagents such as buffers and/or inhibitors, including one or more ofnuclease, protease, and phosphatase inhibitors, which preserve orminimize changes in the molecules in the sample.

Evaluating Biomarkers and Pharmacodynamic Markers

Expression levels of CCNE1 or p16 can be detected as, e.g., RNAexpression of a target gene (i.e., the genes encoding CCNE1 or p16).That is, the expression level (amount) of CCNE1 or p16 can be determinedby detecting and/or measuring the level of mRNA expression of the geneencoding CCNE1. Alternatively, expression levels of CCNE1 or p16 can bedetected as, e.g., protein expression of target gene (i.e., the genesencoding CCNE1 or p16). That is, the expression level (amount) of CCNE1or p16 can be determined by detecting and/or measuring the level ofprotein expression of the genes encoding CCNE1 or p16.

In some embodiments, the expression level of CCNE1 or p16 is determinedby measuring RNA levels. A variety of suitable methods can be employedto detect and/or measure the level of mRNA expression of a gene. Forexample, mRNA expression can be determined using Northern blot or dotblot analysis, reverse transcriptase-PCR (RT-PCR; e.g., quantitativeRT-PCR), in situ hybridization (e.g., quantitative in situhybridization), nucleic acid array (e.g., oligonucleotide arrays or genechips) and RNA sequencing analysis. Details of such methods aredescribed below and in, e.g., Sambrook et al., Molecular Cloning: ALaboratory Manual Second Edition vol. 1, 2 and 3. Cold Spring HarborLaboratory Press: Cold Spring Harbor, N.Y., USA, November 1989; Gibsonet al. (1999) Genome Res., 6(10):995-1001; and Zhang et al. (2005)Environ. Sci. Technol., 39(8):2777-2785; U.S. Publication No.2004086915; European Patent No. 0543942; and U.S. Pat. No. 7,101,663;Kukurba et al. (2015) Cold Spring Harbor Protocols., 2015 (11): 951-69;the disclosures of each of which are incorporated herein by reference intheir entirety.

In one example, the presence or amount of one or more discrete mRNApopulations in a biological sample can be determined by isolating totalmRNA from the biological sample (see, e.g., Sambrook et al. (supra) andU.S. Pat. No. 6,812,341) and subjecting the isolated mRNA to agarose gelelectrophoresis to separate the mRNA by size. The size-separated mRNAsare then transferred (e.g., by diffusion) to a solid support such as anitrocellulose membrane. The presence or amount of one or more mRNApopulations in the biological sample can then be determined using one ormore detectably-labeled-polynucleotide probes, complementary to the mRNAsequence of interest, which bind to and thus render detectable theircorresponding mRNA populations. Detectable-labels include, e.g.,fluorescent (e.g., umbelliferone, fluorescein, fluoresceinisothiocyanate, rhodamine, dichlorotriazinylamine fluorescein, dansylchloride, allophycocyanin, or phycoerythrin), luminescent (e.g.,europium, terbium, Qdot™ nanoparticles supplied by the Quantum DotCorporation, Palo Alto, Calif.), radiological (e.g., 125I, 131I, 35S,32P, 33P, or 3H), and enzymatic (horseradish peroxidase, alkalinephosphatase, beta-galactosidase, or acetylcholinesterase) labels.

In some embodiments, the expression level of CCNE1 or p16 is determinedby measuring protein levels. A variety of suitable methods can beemployed to detect and/or measure the level of protein expression oftarget genes. For example, CCNE1 or p16 protein expression can bedetermined using western blot, enzyme-linked immunosorbent assay(“ELISA”), fluorescence activated cell sorting, or immunohistochemistryanalysis (e.g., using a CCNE1-specific or p16-specific antibody,respectively). Details of such methods are described below and in, e.g.,Sambrook et al., supra.

In one example, the presence or amount of one or more discrete proteinpopulations (e.g., CCNE1 or p16) in a biological sample can bedetermined by western blot analysis, e.g., by isolating total proteinfrom the biological sample (see, e.g., Sambrook et al. (supra)) andsubjecting the isolated protein to agarose gel electrophoresis toseparate the protein by size. The size-separated proteins are thentransferred (e.g., by diffusion) to a solid support such as anitrocellulose membrane. The presence or amount of one or more proteinpopulations in the biological sample can then be determined using one ormore antibody probes, e.g., a first antibody specific for the protein ofinterest (e.g., CCNE1 or p16), and a second antibody, detectablylabeled, specific for the first antibody, which binds to and thusrenders detectable the corresponding protein population.Detectable-labels suitable for use in western blot analysis are known inthe art.

Methods for detecting or measuring gene expression (e.g., mRNA orprotein expression) can optionally be performed in formats that allowfor rapid preparation, processing, and analysis of multiple samples.This can be, for example, in multi-welled assay plates (e.g., 96 wellsor 386 wells) or arrays (e.g., nucleic acid chips or protein chips).Stock solutions for various reagents can be provided manually orrobotically, and subsequent sample preparation (e.g., RT-PCR, labeling,or cell fixation), pipetting, diluting, mixing, distribution, washing,incubating (e.g., hybridization), sample readout, data collection(optical data) and/or analysis (computer aided image analysis) can bedone robotically using commercially available analysis software,robotics, and detection instrumentation capable of detecting the signalgenerated from the assay. Examples of such detectors include, but arenot limited to, spectrophotometers, luminometers, fluorimeters, anddevices that measure radioisotope decay. Exemplary high-throughputcell-based assays (e.g., detecting the presence or level of a targetprotein in a cell) can utilize ArrayScan® VTI HCS Reader or KineticScan®HCS Reader technology (Cellomics Inc., Pittsburgh, Pa.).

In some embodiments, the presence of a CDKN2A gene encoding a p16protein comprising the amino acid sequence of SEQ ID NO:1 and/or thepresence of a CDKN2A gene lacking one or more inactivating nucleic acidsubstitutions and/or deletions is determined by evaluating the DNAsequence of the CDKN2A gene (e.g., genomic DNA or cDNA) or by evaluatingthe RNA sequence of the CDKN2A gene (e.g., RNA, e.g., mRNA). Methods ofperforming nucleic acid sequencing analyses are known in the art anddescribed above. Nonlimiting examples of inactivating nucleic acidsubstitutions and/or deletions preventing the CDKN2A gene from encodinga protein comprising the amino acid sequence of SEQ ID NO:1 aredescribed in Table A, above. In specific embodiments, the one or moreinactivating nucleic acid substitutions and/or deletions in the CDKN2Agene is as described in Yarbrough et al., Journal of the National CancerInstitute, 91(18):1569-1574, 1999; Liggett and Sidransky, Biology ofNeoplasia, Journal of Oncology, 16(3):1197-1206, 1998, and Cairns etal., Nature Genetics, 11:210-212, 1995, each of which is incorporated byreference herein in its entirety.

In some embodiments, the expression level of a gene or the presence of agene lacking one or more inactivating nucleic acid substitutions ordeletions is determined by evaluating the copy number variation (CNV) ofthe gene. The CNV of genes (e.g., the CCNE1 gene and/or the CDKN2A gene)can be determined/identified by a variety of suitable methods. Forexample, CNV can be determined using fluorescent in situ hybridization(FISH), multiplex ligation dependent probe amplification (MLPA), arraycomparative genomic hybridization (aCGH), single-nucleotidepolymorphisms (SNP) array, and next-generation sequencing (NGS)technologies.

In one example, the copy number variation of one or more discrete genesin a biological sample can be determined by MLPA, e.g., by extractingDNA specimens from the biological sample (see, e.g., Sambrook et al.(supra) and U.S. Pat. No. 6,812,341), and amplifying DNA sequence ofinterest (e.g., CCNE1 or CDKN2A) using a mixture of MLPA probes. EachMLPA probe consists of two oligonucleotides that hybridize toimmediately adjacent target DNA sequence (e.g., CCNE1 or CDKN2A) inorder to be ligated into a single probe. Ligated probes are amplifiedthough PCR with one PCR primer fluorescently labeled, enabling theamplification products to be visualized during fragment separation bycapillary electrophoresis. The presence, absence or amplification of oneor more genes of interest in the biological sample is calculated bymeasuring PCR derived fluorescence, quantifying the amount of PCRproduct after normalization and comparing it with control DNA samples.

The level of Rb phosphorylation at the serine corresponding to aminoacid position 780 of SEQ ID NO:3 can be detected by a variety ofsuitable methods. For example, phosphorylation status can be determinedusing western blot, ELISA, fluorescence activated cell sorting, orimmunohistochemistry analysis. Details of such methods are describedbelow and in, e.g., Sambrook et al., supra.

As with the methods for detecting or measuring gene expression (above),methods for detecting or measuring the level of Rb phosphorylation atthe serine corresponding to amino acid position 780 of SEQ ID NO:3 canoptionally be performed in formats that allow for rapid preparation,processing, and analysis of multiple samples.

The invention will be described in greater detail by way of specificexamples. The following examples are offered for illustrative purposes,and are not intended to limit the invention in any manner. Those ofskill in the art will readily recognize a variety of non-criticalparameters which can be changed or modified to yield essentially thesame results.

EXAMPLES

Experimental procedures for compounds of the invention are providedbelow. Preparatory LC-MS purifications of some of the compounds preparedwere performed on Waters mass directed fractionation systems. The basicequipment setup, protocols, and control software for the operation ofthese systems have been described in detail in the literature. See e.g.,“Two-Pump at-Column Dilution Configuration for Preparative LC-MS”, K.Blom, J. Combi. Chem., 4, 295 (2002); “Optimizing Preparative LC-MSConfigurations and Methods for Parallel Synthesis Purification”, K.Blom, R. Sparks, J. Doughty, G. Everlof, T. Haque, A. Combs, J Combi.Chem., 5, 670 (2003); and “Preparative LC-MS Purification: ImprovedCompound Specific Method Optimization”, K. Blom, B. Glass, R. Sparks, A.Combs, J Combi. Chem., 6, 874-883 (2004). The separated compounds weretypically subjected to analytical liquid chromatography massspectrometry (LCMS) for purity check under the following conditions:Instrument; Agilent 1100 series, LC/MSD, Column: Waters Sunfire™ C₁₈ 5μm particle size, 2.1×5.0 mm, Buffers: mobile phase A: 0.025% TFA inwater and mobile phase B: acetonitrile; gradient 2% to 80% of B in 3minutes with flow rate 2.0 mL/minute.

Some of the compounds prepared were also separated on a preparativescale by reverse-phase high performance liquid chromatography (RP-HPLC)with MS detector or flash chromatography (silica gel) as indicated inthe Examples. Typical preparative reverse-phase high performance liquidchromatography (RP-HPLC) column conditions are as follows:

pH=2 purifications: Waters Sunfire™ C₁₈ 5 μm particle size, 19×100 mmcolumn, eluting with mobile phase A: 0.1% TFA (trifluoroacetic acid) inwater and mobile phase B: acetonitrile; the flow rate was 30 mL/minute,the separating gradient was optimized for each compound using theCompound Specific Method Optimization protocol as described in theliterature (see “Preparative LCMS Purification: Improved CompoundSpecific Method Optimization”, K. Blom, B. Glass, R. Sparks, A. Combs,J. Comb. Chem., 6, 874-883 (2004)). Typically, the flow rate used withthe 30×100 mm column was 60 mL/minute.

pH=10 purifications: Waters XBridge Cis 5 μm particle size, 19×100 mmcolumn, eluting with mobile phase A: 0.15% NH₄OH in water and mobilephase B: acetonitrile; the flow rate was 30 mL/minute, the separatinggradient was optimized for each compound using the Compound SpecificMethod Optimization protocol as described in the literature (See“Preparative LCMS Purification: Improved Compound Specific MethodOptimization”, K. Blom, B. Glass, R. Sparks, A. Combs, J. Comb. Chem.,6, 874-883 (2004)). Typically, the flow rate used with 30×100 mm columnwas 60 mL/minute.

Intermediate 1. 2-Methyl-3,5,6,7-tetrahydro-4H-benzo[d]imidazol-4-one

Step 1: 2-Bromo-3-ethoxycyclohex-2-en-1-one

In a flask with a stir bar, a mixture of 3-ethoxycyclohex-2-en-1-one(Alfa Aesar, 9.84 g, 70.2 mmol) and N-bromosuccinimide (13.1 g, 73.6mmol) in CH₂Cl₂ (140 mL) was stirred at room temperature for 36 hours.The resultant mixture was quenched by saturated aqueous NaHCO₃,extracted by CH₂Cl₂ (500 mL×2), and the combined organic layers weredried over anhydrous Na₂SO₄, filtered, and concentrated. The residue wasthen slurry washed by hexanes, and filtered. The filtrate was discarded.The solid was dried in vacuo to afford crude2-bromo-3-ethoxycyclohex-2-en-1-one (15.3 g), which was used nextreaction without further purification. LCMS calculated for C₈H₁₂BrO₂(M+H)⁺: m/z=219.0; Found 219.0.

Step 2: 2-Methyl-3,5,6,7-tetrahydro-4H-benzo[d]imidazol-4-one

In a flask with a stir bar, a mixture of2-bromo-3-ethoxycyclohex-2-en-1-one (15.3 g), acetimidamidehydrochloride (7.96 g, 84.2 mmol), and potassium carbonate (35.0 g, 253mmol) in DMF (150 ml) was stirred at 110° C. for 2 hours. The solutionwas concentrated under reduced pressure, and the mixture was thenpurified via flash column chromatography (Agela Flash Column Silica-CS(120 g), eluting with a gradient of 0 to 20% CH₂Cl₂/methanol) to afford2-methyl-3,5,6,7-tetrahydro-4H-benzo[d]imidazol-4-one (3.34 g, 22.2mmol, 32% yield). LCMS calculated for C₈H₁₁N₂O (M+H)⁺: m/z=151.1; Found151.0.

Intermediate 2.2,3-Dimethyl-3,5,6,7-tetrahydro-4H-benzo[d]imidazol-4-one

In a flask with a stir bar, a mixture of2-methyl-3,5,6,7-tetrahydro-4H-benzo[d]imidazol-4-one (1.75 g, 11.7mmol) and Cs₂CO₃ (4.18 g, 12.8 mmol) in DMF (140 ml) was stirred at roomtemperature for 10 min. MeI (2 M in tert-BuOMe, 5.85 mL, 11.7 mmol) wasadded to the solution, and stirred at 0° C. for 3 hours. Then saturatedaqueous NH₄Cl solution was added to the mixture, and the resultantmixture was extracted by CH₂Cl₂ (600 mL×2), combined organic layers werewashed with brine (100 mL), dried over anhydrous Na₂SO₄, andconcentrated. The residue was purified via flash column chromatography(Agela Flash Column Silica-CS (120 g), eluting with a gradient of 0 to20% CH₂C2/methanol) to afford2,3-dimethyl-3,5,6,7-tetrahydro-4H-benzo[d]imidazol-4-one (1.15 g, 7.00mmol, 60.1% yield). LCMS calculated for C₉H₁₃N₂O (M+H)⁺: m/z=165.1;Found 165.1.

Intermediate 3. tert-Butyl4-((1,2-dimethyl-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-yl)amino)piperidine-1-carboxylate

A mixture of 2,3-dimethyl-3,5,6,7-tetrahydro-4H-benzo[d]imidazol-4-one(490 mg, 2.98 mmol) and1-tert-butoxy-N,N,N′,N′-tetramethylmethanediamine (4.0 mL, 19 mmol) waspurged with nitrogen and irradiated in a microwave reactor at 140° C.for 1 hour. After the resultant mixture was quenched by MeOH, themixture was concentrated in vacuo. The residue was added to tert-butyl4-guanidinopiperidine-1-carboxylate hydrochloride (723 mg, 2.98 mmol) inEtOH (2 mL), and the mixture was purged with nitrogen and irradiated ina microwave reactor at 120° C. for 1 hour. The resultant mixture wasfiltered and concentrated in vacuo. The residue was purified via flashcolumn chromatography (Agela Flash Column Silica-CS (40 g), eluting witha gradient of 0 to 3% CH₂Cl₂/methanol) to afford tert-butyl4-((1,2-dimethyl-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-yl)amino)piperidine-1-carboxylate(440 mg, 1.10 mmol, 37% yield). LCMS calculated for C₂₁H₃₁N₆O₂ (M+H)⁺:m/z=399.3; Found 399.5.

Intermediate 4. 1-(1-(Methylsulfonyl)piperidin-4-yl)guanidinehydrochloride

A mixture of 1H-pyrazole-1-carboximidamide hydrochloride (13.9 g, 95mmol), 1-(methylsulfonyl)piperidin-4-amine (16.90 g, 95 mmol) and DIPEA(16.56 mL, 95 mmol) in DMF (60 mL) was stirred at 60° C. for 18 h. Et₂O(400 mL) was added to precipitate the desired product as white solid.LCMS calculated for C₇H₁₇N₄O₂S (M−HCl+H)⁺: m/z=221.1; Found 221.0.

Example 1.N-(1-(Ethylsulfonyl)piperidin-4-yl)-1,2-dimethyl-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine

In a screw-cap vial equipped with a magnetic stir bar, trifluoroaceticacid (3 mL) was added to tert-butyl4-((1,2-dimethyl-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-yl)amino)piperidine-1-carboxylate(440 mg, 1.104 mmol). After stirring at room temperature for 1 hour,trifluoroacetic acid was removed under reduced pressure to afford crude1,2-dimethyl-N-(piperidin-4-yl)-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amineas the trifluoroacetic acid salt. To a solution of the obtained crudematerial (100 mg) in CH₂Cl₂ (3 mL) was added N,N-diisopropylethylamine(0.75 mL, 4.3 mmol) and ethanesulfonyl chloride. After the mixture wasstirred at room temperature for 1 hour, the solution was quenched byMeOH, and the resulting solution was concentrated. The residue wasdissolved in acetonitrile for prep-LCMS (XBridge C18 column, elutingwith a gradient of acetonitrile/water containing 0.1% NH₄OH, at flowrate of 60 m/min). LCMS calculated for C₁₈H₂₇N₆O₂S (M+H)⁺: m/z=391.2;Found 391.1.

Example 2.N-(1-(Cyclopropylsulfonyl)piperidin-4-yl)-1,2-dimethyl-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine

This compound was prepared according to the procedures described inExample 1, using cyclopropanesulfonyl chloride instead of ethanesulfonylchloride as starting material. CMS calculated for C₁₉H₂₇N₆O₂S (M+H)⁺:m/z=403.2; Found 403.1.

Example 3.1,2-Dimethyl-N-(1-((1-methyl-1H-pyrazol-4-yl)sulfonyl)piperidin-4-yl)-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine

This compound was prepared according to the procedures described inExample 1, using 1-methyl-1H-pyrazole-4-sulfonyl chloride instead ofethanesulfonyl chloride as starting material. LCMS calculated forC₂H₂₇N₈O₂S (M+H)⁺: m/z=443.2; Found 443.3.

Example 4.N-(1-((1H-Pyrazol-4-yl)sulfonyl)piperidin-4-yl)-1,2-dimethyl-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine

This compound was prepared according to the procedures described inExample 1, using 1H-pyrazole-4-sulfonyl chloride instead ofethanesulfonyl chloride as starting material. LCMS calculated forC₁₉H₂₅N₈O₂S (M+H)⁺: m/z=429.2; Found 429.1.

Example 5.3-((4-((1,2-Dimethyl-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-yl)amino)piperidin-1-yl)sulfonyl)benzonitrile

This compound was prepared according to the procedures described inExample 1, using 3-cyanobenzenesulfonyl chloride instead ofethanesulfonyl chloride as starting material. LCMS calculated forC₂₃H₂₆N₇O₂S (M+H)⁺: m/z=464.2; Found 464.1.

Example 6.N-(1-((1,2-Dimethyl-1H-imidazol-4-yl)sulfonyl)piperidin-4-yl)-1,2-dimethyl-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine

This compound was prepared according to the procedures described inExample 1, using 1,2-dimethyl-1H-imidazole-4-sulfonyl chloride insteadof ethanesulfonyl chloride as starting material. LCMS calculated forC₂₁H₂₉N₈O₂S (M+H)⁺: m/z=457.2; Found 457.5.

Example 7.2-Methyl-N-(1-(methylsulfonyl)piperidin-4-yl)-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine

A mixture of 2-methyl-3,5,6,7-tetrahydro-4H-benzo[d]imidazol-4-one (90mg, 0.60 mmol) and 1-tert-butoxy-N,N,N′,N′-tetramethylmethanediamine(2.0 mL, 9.7 mmol) was purged with nitrogen and irradiated in amicrowave reactor at 140° C. for 1 hour. After the resultant mixture wasquenched by MeOH, the mixture was concentrated in vacuo. The residue wasthen dissolved in EtOH (1 mL), and the solution was added to a mixtureof sodium ethoxide (21 wt % in ethanol, 0.19 mL, 0.57 mmol) and1-(1-(methylsulfonyl)piperidin-4-yl)guanidine hydrochloride (129 mg,0.599 mmol). The solution was purged with nitrogen and irradiated in amicrowave reactor at 120° C. for 1 hour. The resultant mixture wasfiltered and concentrated in vacuo. The residue was dissolved inacetonitrile and purified by prep-LCMS (Sunfire C18 column, eluting withgradient of acetonitrile/water containing 0.1% TFA, at flow rate of 60mL/min). The fractions containing product were then concentrated andfurther purified by prep-LCMS (XBridge C18 column, eluting with agradient of acetonitrile/water containing 0.1% NH₄OH, at flow rate of 60mL/min). LCMS calculated for C₁₆H₂₃N₆O₂S (M+H)⁺: m/z=363.2; Found 363.1.

Example 8.1-Benzyl-2-methyl-N-(1-(methylsulfonyl)piperidin-4-yl)-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine

Step 1: 3-Benzyl-2-methyl-3,5,6,7-tetrahydro-4H-benzo[d]imidazol-4-one

A screw-cap vial equipped with a magnetic stir bar was charged with2-methyl-3,5,6,7-tetrahydro-4H-benzo[d]imidazol-4-one (300 mg, 2.00mmol) and Cs₂CO₃ (716 mg, 2.20 mmol) in DMF (5 mL). Benzyl chloride(0.25 mL, 2.2 mmol) and sodium iodide (59.9 mg, 0.400 mmol) were addedto the solution respectively, and stirred at room temperature for 24hours. Then saturated aqueous NH₄Cl sol. (20 mL) was added to themixture, and the resultant mixture was extracted by CH₂Cl₂ (50 mL×2),and the combined organic layers were washed with brine (50 mL), driedwith anhydrous Na₂SO₄, and concentrated. The residue was purified viaflash column chromatography (Agela Flash Column Silica-CS (40 g),eluting with a gradient of 0 to 20% CH₂C2/methanol) to afford twoisomers of3-benzyl-2-methyl-3,5,6,7-tetrahydro-4H-benzo[d]imidazol-4-one and1-benzyl-2-methyl-1,5,6,7-tetrahydro-4H-benzo[d]imidazol-4-one (Total:480 mg, 2.00 mmol, 100% yield). LCMS calculated for C₁₅H₁₇N₂O (M+H)⁺:m/z=241.1; Found 241.2.

Step 2:1-Benzyl-2-methyl-N-(1-(methylsulfonyl)piperidin-4-yl)-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine

A mixture of3-benzyl-2-methyl-3,5,6,7-tetrahydro-4H-benzo[d]imidazol-4-one and1-benzyl-2-methyl-1,5,6,7-tetrahydro-4H-benzo[d]imidazol-4-one (Total:120 mg, 0.499 mmol) and1-tert-butoxy-N,N,N′,N′-tetramethylmethanediamine (2.0 mL, 9.7 mmol) waspurged with nitrogen and irradiated in a microwave reactor at 140° C.for 1 hour. After the resultant mixture was quenched by MeOH, themixture was concentrated in vacuo. The residue was then dissolved inEtOH (10 ml), and the solution was added to a mixture of sodium ethoxide(21 wt % in ethanol, 0.16 mL, 0.50 mmol) and1-(1-(methylsulfonyl)piperidin-4-yl)guanidine hydrochloride (128 mg,0.499 mmol). The solution was purged with nitrogen and irradiated in amicrowave reactor at 120° C. for 1 hour. The resultant mixture wasfiltered and concentrated in vacuo. The residue was dissolved inacetonitrile and purified by prep-LCMS (XBridge column, eluting with agradient of acetonitrile/water containing 0.1% NH₄OH, at flow rate of 60m/min). Fractions containing the desired product were then concentrated,and the material obtained was dissolved in acetonitrile and purified byprep-LCMS (Sunfire C18 column, eluting with a gradient ofacetonitrile/water containing 0.1% TFA, at flow rate of 60 mL/min). ¹HNMR (TFA salt, 600 MHz, DMSO-d₆, 343 K) δ 8.13 (s, 1H), 7.38 (m, 2H),7.31 (m, 1H), 7.21 (m, 2H), 5.96 (s, 2H), 3.59 (m, 1H), 3.42 (m, 2H),2.91 (m, 4H), 2.80 (s, 3H), 2.55-2.45 (m, 2H), 2.43 (s, 3H), 1.77 (m,2H), 1.46 (m, 2H). LCMS calculated for C₂₃H₂₉N₆O₂S (M+H)⁺: m/z=453.2;Found 453.2.

Example 9.8-Methyl-N-(1-(methylsulfonyl)piperidin-4-yl)-6,8-dihydro-5H-pyrazolo[3,4-h]quinazolin-2-amine

Step 1: 8-Methyl-6,8-dihydro-5H-pyrazolo[3,4-h]quinazolin-2-amine

A solution of cyclohexane-1,3-dione (147 mg, 1.31 mmol) andmethylhydrazine (0.069 mL, 1.31 mmol) in THF (1 mL) was stirred at RTfor 2 h. 1,1-Dimethoxy-N,N-dimethylmethanamine (1562 mg, 13.11 mmol) wasadded at RT. The mixture was purged with nitrogen and irradiated in amicrowave reactor at 120° C. for 30 min. Then, the resultant mixture wasconcentrated and added guanidine hydrochloride (1252 mg, 13.11 mmol) andNaOEt in EtOH (21 wt %, 4.89 mL, 13.11 mmol). The mixture was purgedwith nitrogen and irradiated in a microwave reactor at 140° C. for 30min. The resultant mixture was then purified by prep-LCMS (XBridge C18column, eluting with a gradient of acetonitrile/water containing 0.1%NH₄OH, at flow rate of 60 m/min). LCMS calculated for C₁₀H₁₂N₅ (M+H)⁺.m/z=202.1; Found 202.1.

Step 2:8-Methyl-N-(1-(methylsulfonyl)piperidin-4-yl)-6,8-dihydro-5H-pyrazolo[3,4-h]quinazolin-2-amine

A mixture of 8-methyl-6,8-dihydro-5H-pyrazolo[3,4-h]quinazolin-2-amine(10 mg, 0.050 mmol), 4-bromo-1-(methylsulfonyl)piperidine (120 mg, 0.497mmol) and cesium carbonate (162 mg, 0.497 mmol) in DMF (1 mL) wasstirred at 100° C. for 18 h. The resultant mixture was then purified byprep-LCMS (Sunfire C18 column, eluting with a gradient ofacetonitrile/water containing 0.1% TFA, at flow rate of 60 mL/min). LCMScalculated for C₁₆H₂₃N₆O₂S (M+H)⁺: m/z=363.2; Found 363.1.

Example 10.8-Methyl-N-(1-(methylsulfonyl)piperidin-4-yl)-6,8-dihydropyrazolo[4′,3′:4,5]pyrano[3,2-d]pyrimidin-2-amine

This compound was prepared according to the procedures described inExample 9, using 2H-pyran-3,5(4H,6H)-dione instead ofcyclohexane-1,3-dione as starting material. LCMS calculated forC₁₅H₂₁N₆O₃S (M+H)⁺: m/z=365.1; Found 365.0.

Example 11.8-(2,6-Difluorophenyl)-9-methyl-N-(1-(methylsulfonyl)piperidin-4-yl)-6,8-dihydro-5H-pyrazolo[3,4-h]quinazolin-2-amine

Step1:2-(2,6-Difluorophenyl)-3-methyl-2,5,6,7-tetrahydro-4H-indazol-4-one

A solution of cyclohexane-1,3-dione (160 mg, 1.42 mmol) and(2,6-difluorophenyl)hydrazine (206 mg, 1.42 mmol) in THE (1 mL) wasstirred at RT for 2 h. Then 1,1-dimethoxy-N,N-dimethylethan-1-amine (417μL, 2.85 mmol) was added. The mixture was purged with nitrogen andirradiated in a microwave reactor at 120° C. for 30 min. The resultantmixture was then purified by prep-LCMS (XBridge C18 column, eluting witha gradient of acetonitrile/water containing 0.1% NH₄OH, at flow rate of60 mL/min). LCMS calculated for C₁₄H₁₃F₂N₂O (M+H)⁺: m/z=263.1; Found263.2.

Step 2:8-(2,6-Difluorophenyl)-9-methyl-N-(1-(methylsulfonyl)piperidin-4-yl)-6,8-dihydro-5H-pyrazolo[3,4-h]quinazolin-2-amine

This compound was prepared according to the procedures described inExample 7, using2-(2,6-difluorophenyl)-3-methyl-2,5,6,7-tetrahydro-4H-indazol-4-oneinstead of 2-methyl-3,5,6,7-tetrahydro-4H-benzo[d]imidazol-4-one asstarting material. LCMS calculated for C₂₂H₂₅F₂N₆O₂S (M+H)⁺: m/z=475.2;Found 475.0.

Example 12.9-Cyclopropyl-8-methyl-N-(1-(methylsulfonyl)piperidin-4-yl)-6,8-dihydro-5H-pyrazolo[3,4-h]quinazolin-2-amine

Step 1: 3-Oxocyclohex-1-en-1-yl cyclopropanecarboxylate

To a solution of cyclohexane-1,3-dione (1.83 g, 16.3 mmol) andtriethylamine (2.96 mL, 21.2 mmol) in THF (15 mL) was addedcyclopropanecarbonyl chloride (1.64 mL, 17.95 mmol) at RT. The mixturewas stirred at RT for 2 h. The resultant mixture was concentrated underreduced pressure, and purified via flash column chromatography to givethe desired product. LCMS calculated for C₁₀H₁₃O₃ (M+H)⁺: m/z=181.1;Found 181.0.

Step 2: 2-(Cyclopropanecarbonyl)cyclohexane-1,3-dione

To a solution of 3-oxocyclohex-1-en-1-yl cyclopropanecarboxylate (2.62g, 14.5 mmol) in DCM (15 mL) was added aluminum chloride (3.88 g, 29.1mmol) at 0° C. The mixture was stirred at RT for 2 h and then aq. HCl(1.0 M, 20 mL) was added. Using a separatory funnel, the aqueous layerwas extracted with DCM (20 mL×3). The combined organic fractions weredried over Na₂SO₄, concentrated under reduced pressure, and purified viaflash column chromatography to give the desired product. LCMS calculatedfor C₁₀H₁₃O₃ (M+H)⁺: m/z=181.1; Found 181.0.

Step 3: tert-Butyl2-(2-(cyclopropanecarbonyl)-3-oxocyclohex-1-en-1-yl)-1-methylhydrazine-1-carboxylate

To a solution of 2-(cyclopropanecarbonyl)cyclohexane-1,3-dione (178 mg,0.988 mmol) in DCM (2 mL) was added oxalyl chloride (0.173 mL, 1.976mmol) at RT. The mixture was stirred at RT for 18 h and thenconcentrated under reduced pressure. The crude material and tert-butyl1-methylhydrazine-1-carboxylate (0.147 mL, 0.988 mmol), triethylamine(0.275 mL, 1.976 mmol) were dissolved in THE (2 mL). The resultantmixture was stirred at RT for 18 h and then water (5 mL) was added.Using a separatory funnel, the aqueous layer was extracted with DCM (20mL×3). The combined organic fractions were dried over Na₂SO₄,concentrated under reduced pressure, and purified via flash columnchromatography to give the desired product. LCMS calculated forC₁₆H₂₅N₂O₄ (M+H)⁺: m/z=309.2; Found 309.1.

Step 4: 3-Cyclopropyl-2-methyl-2,5,6,7-tetrahydro-4H-indazol-4-one

To tert-butyl2-(2-(cyclopropanecarbonyl)-3-oxocyclohex-1-en-1-yl)-1-methylhydrazine-1-carboxylate(from Step 3) was added HCl in dioxane (4 M, 3 mL) at RT. The resultantsolution was stirred at RT overnight. The resultant mixture wasconcentrated under reduced pressure and the crude product was useddirectly for the next step. LCMS calculated for C₁₁H₁₅N₂O (M+H)⁺:m/z=191.1; Found 191.0.

Step 5:9-Cyclopropyl-8-methyl-N-(1-(methylsulfonyl)piperidin-4-yl)-6,8-dihydro-5H-pyrazolo[3,4-h]quinazolin-2-amine

This compound was prepared according to the procedures described inExample 7, using3-cyclopropyl-2-methyl-2,5,6,7-tetrahydro-4H-indazol-4-one instead of2-methyl-3,5,6,7-tetrahydro-4H-benzo[d]imidazol-4-one as startingmaterial. LCMS calculated for C₁₉H₂₇N₆O₂S (M+H)⁺: m/z=403.2; Found403.0.

Example 13.9-Isopropyl-8-methyl-N-(1-(methylsulfonyl)piperidin-4-yl)-6,8-dihydro-5H-pyrazolo[3,4-h]quinazolin-2-amine

This compound was prepared according to the procedures described inExample 12, using isobutyryl chloride instead of cyclopropanecarbonylchloride as starting material. LCMS calculated for C₁₉H₂₉N₆O₂S (M+H)⁺:m/z=405.2; Found 405.1. ¹H NMR (TFA salt, 500 MHz, DMSO-d₆) δ 8.10 (s,1H), 3.95 (m, 1H), 3.87 (s, 3H), 3.75 (m, 1H), 3.62 (d, J=12.3 Hz, 2H),2.93 (s, 3H), 2.87-2.75 (m, 4H), 2.71 (t, J=6.8 Hz, 2H), 2.01 (d, J=10.5Hz, 2H), 1.64 (td, J=15.0, 4.0 Hz, 2H), 1.43 (d, J=7.1 Hz, 6H).

Example 14.9-Cyclopropyl-N-(1-(methylsulfonyl)piperidin-4-yl)-8-(tetrahydrofuran-3-yl)-6,8-dihydro-5H-pyrazolo[3,4-h]quinazolin-2-amine

This compound was prepared according to the procedures described inExample 12, using tert-butyl1-(tetrahydrofuran-3-yl)hydrazine-1-carboxylate instead of tert-butyl1-methylhydrazine-1-carboxylate as starting material. LCMS calculatedfor C₂₂H₃₁N₆O₃S (M+H)⁺: m/z=459.2; Found 459.0.

Example 15.2-(9-Cyclopropyl-2-((1-(methylsulfonyl)piperidin-4-yl)amino)-5,6-dihydro-8H-pyrazolo[3,4-h]quinazolin-8-yl)ethan-1-ol

This compound was prepared according to the procedures described inExample 12, using tert-butyl 1-(2-hydroxyethyl)hydrazine-1-carboxylateinstead of tert-butyl 1-methylhydrazine-1-carboxylate as startingmaterial. LCMS calculated for C₂H₂₉N₆O₃S (M+H)⁺: m/z=433.2; Found 433.0.

Example 16.8,9-Dicyclopropyl-N-(1-(methylsulfonyl)piperidin-4-yl)-6,8-dihydro-5H-pyrazolo[3,4-h]quinazolin-2-amine

This compound was prepared according to the procedures described inExample 12, using tert-butyl 1-cyclopropylhydrazine-1-carboxylateinstead of tert-butyl 1-methylhydrazine-1-carboxylate as startingmaterial. LCMS calculated for C₂₁H₂₉N₆O₂S (M+H)⁺: m/z=429.2; Found429.0.

Example 17.1,2-Dimethyl-N-(1-(methylsulfonyl)piperidin-4-yl)-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine

This compound was prepared according to the procedures described inExample 7, using2,3-dimethyl-3,5,6,7-tetrahydro-4H-benzo[d]imidazol-4-one instead of2-methyl-3,5,6,7-tetrahydro-4H-benzo[d]imidazol-4-one as startingmaterial. LCMS calculated for C₁₇H₂₅N₆O₂S (M+H)⁺: m/z=377.2; Found377.0.

Example 18.2-Isopropyl-1-methyl-N-(1-(methylsulfonyl)piperidin-4-yl)-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine

Step 1: 2-Isopropyl-3,5,6,7-tetrahydro-4H-benzo[d]imidazol-4-one

This compound was prepared according to the procedures described inIntermediate 1, using isobutyrimidamide hydrochloride instead ofacetimidamide hydrochloride as starting material. LCMS calculated forC₁₀H₁₅N₂O (M+H)⁺: m/z=179.1; Found 179.0.

Step 2:2-Isopropyl-1-methyl-N-(1-(methylsulfonyl)piperidin-4-yl)-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine

This compound was prepared according to the procedures described inIntermediate 2 and Example 7, using2-isopropyl-3,5,6,7-tetrahydro-4H-benzo[d]imidazol-4-one instead of2-methyl-3,5,6,7-tetrahydro-4H-benzo[d]imidazol-4-one as startingmaterial. LCMS calculated for C₁₉H₂₉N₆O₂S (M+H)⁺: m/z=405.2; Found405.1.

Example 19.1-Cyclopropyl-2-methyl-N-(1-(methylsulfonyl)piperidin-4-yl)-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine

Step1:3-Cyclopropyl-2-methyl-3,5,6,7-tetrahydro-4H-benzo[d]imidazol-4-one

A solution of copper (II) acetate (48.4 mg, 0.266 mmol) and2,2′-bipyridine (41.6 mg, 0.266 mmol) in 1,2-dichloroethane (2 mL) wasstirred at 60° C. for 15 min. Then the resultant mixture was added to asolution of 2-methyl-3,5,6,7-tetrahydro-4H-benzo[d]imidazol-4-one (40mg, 0.266 mmol), cyclopropylboronic acid (34.3 mg, 0.400 mmol) andpotassium carbonate (73.6 mg, 0.533 mmol) in 1,2-dichloroethane (2 mL)at RT. The mixture was stirred under air at 60° C. for 18 h. Theresultant mixture was then purified by prep-LCMS (XBridge C18 column,eluting with a gradient of acetonitrile/water containing 0.1% NH₄OH, atflow rate of 60 m/min). LCMS calculated for C₁₁H₁₅N₂O (M+H)⁺: m/z=191.1;Found 191.1.

Step 2:1-Cyclopropyl-2-methyl-N-(1-(methylsulfonyl)piperidin-4-yl)-4,5-dihydro-H-imidazo[4,5-h]quinazolin-8-amine

This compound was prepared according to the procedures described inExample 7, using3-cyclopropyl-2-methyl-3,5,6,7-tetrahydro-4H-benzo[d]imidazol-4-oneinstead of 2-methyl-3,5,6,7-tetrahydro-4H-benzo[d]imidazol-4-one asstarting material. LCMS calculated for C₁₉H₂₇N₆O₂S (M+H)⁺: m/z=403.2;Found 403.2. ¹H NMR (TFA salt, 500 MHz, DMSO-d₆) δ 8.16 (s, 1H), 3.95(m, 1H), 3.56 (m, 3H), 2.89 (s, 3H), 2.87 (m, 6H), 2.65 (s, 3H), 1.99(d, J=10.1 Hz, 2H), 1.60 (m, 2H), 1.27 (m, 2H), 1.06 (m, 2H).

Example 20.1-Cyclopropyl-N-(1-(methylsulfonyl)piperidin-4-yl)-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine

Step 1: 3-Cyclopropyl-3,5,6,7-tetrahydro-4H-benzo[d]imidazol-4-one

To a solution of ethyl formimidate hydrochloride (0.90 g, 8.22 mmol) iniPrOH (10 mL) was added triethylamine (2.29 mL, 16.43 mmol) at 0° C. Themixture was stirred at RT for 15 min. Cyclopropanamine (0.569 mL, 8.22mmol) was added at 0° C. The mixture was stirred at RT for 2 h.2-Bromo-3-ethoxycyclohex-2-en-1-one (1.44 g, 6.57 mmol) was added at RT.The mixture was stirred at 80° C. for 18 h. The resultant mixture wasconcentrated under reduced pressure, and purified via flash columnchromatography to give the desired product. LCMS calculated for C₁₀HN₂O(M+H)⁺: m/z=177.1; Found 177.0.

Step 2:1-Cyclopropyl-N-(1-(methylsulfonyl)piperidin-4-yl)-4,5-dihydro-H-imidazo[4,5-h]quinazolin-8-amine

This compound was prepared according to the procedures described inExample 7, using3-cyclopropyl-3,5,6,7-tetrahydro-4H-benzo[d]imidazol-4-one instead of2-methyl-3,5,6,7-tetrahydro-4H-benzo[d]imidazol-4-one as startingmaterial. LCMS calculated for C₁₈H₂₅N₆O₂S (M+H)⁺: m/z=389.2; Found389.1.

Example 21.2-Bromo-1-cyclopropyl-N-(1-(methylsulfonyl)piperidin-4-yl)-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine

In a vial wrapped by aluminum foil, to a solution of1-cyclopropyl-N-(1-(methylsulfonyl)piperidin-4-yl)-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine(7.2 mg, 0.019 mmol) in acetonitrile (1 mL) was added NBS (3.30 mg,0.019 mmol) at RT. The mixture was stirred at RT for 2 h. The resultantmixture was purified by prep-LCMS (Sunfire C18 column, eluting with agradient of acetonitrile/water containing 0.1% TFA, at flow rate of 60mL/min). LCMS calculated for C₁₈H₂₄BrN₆O₂S (M+H)⁺: m/z=467.1; Found467.2.

Example 22.1-Cyclopropyl-N-(1-(methylsulfonyl)piperidin-4-yl)-2-phenyl-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine

A solution of2-bromo-1-cyclopropyl-N-(1-(methylsulfonyl)piperidin-4-yl)-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine(15 mg, 0.032 mmol), phenylboronic acid (7.83 mg, 0.064 mmol),Pd(dppf)Cl₂ (11.74 mg, 0.016 mmol) and Na₃PO₄ (20.44 mg, 0.096 mmol) indioxane (1 mL) and water (0.2 mL) was stirred at 80° C. for 1 h. Theresultant mixture was purified by prep-LCMS (Sunfire C18 column, elutingwith a gradient of acetonitrile/water containing 0.1% TFA, at flow rateof 60 mL/min). LCMS calculated for C₂₄H₂₉N₆O₂S (M+H)⁺: m/z=465.2; Found465.0.

Example 23.1-Cyclopropyl-2-(2-fluorophenyl)-N-(1-(methylsulfonyl)piperidin-4-yl)-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine

This compound was prepared according to the procedures described inExample 22, using (2-fluorophenyl)boronic acid instead of phenylboronicacid as starting material. LCMS calculated for C₂₄H₂₈FN₆O₂S (M+H)⁺:m/z=483.2; Found 483.1.

Example 24.1-Cyclopropyl-2-(1,3-dimethyl-1H-pyrazol-4-yl)-N-(1-(methylsulfonyl)piperidin-4-yl)-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine

This compound was prepared according to the procedures described inExample 22, using1,3-dimethyl-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-1H-pyrazoleinstead of phenylboronic acid as starting material. LCMS calculated forC₂₃H₃₁N₈O₂S (M+H)⁺: m/z=483.2; Found 483.0.

Example 25.1-Cyclopropyl-2,4,4-trimethyl-N-(1-(methylsulfonyl)piperidin-4-yl)-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine

Step 1: 3-Methoxy-4,4-dimethylcyclohex-2-en-1-one

A solution of 4,4-dimethylcyclohexane-1,3-dione (1.23 g, 8.76 mmol) andp-toluenesulfonic acid monohydrate (0.083 g, 0.438 mmol) in methanol (15mL) was stirred at 80° C. for 1 h. The resultant mixture wasconcentrated under reduced pressure, and purified via flash columnchromatography to give the desired product. LCMS calculated for C₉H₁₅O₂(M+H)⁺: m/z=155.1; Found 155.0.

Step 2:1-Cyclopropyl-2,4,4-trimethyl-N-(1-(methylsulfonyl)piperidin-4-yl)-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine

This compound was prepared according to the procedures described inIntermediate 1 and Example 19, using3-methoxy-4,4-dimethylcyclohex-2-en-1-one instead of3-ethoxycyclohex-2-en-1-one as starting material. LCMS calculated forC₂₁H₃₁N₆O₂S (M+H)⁺: m/z=431.2; Found 431.0.

Example 26.1-Cyclopropyl-N-(1-(methylsulfonyl)piperidin-4-yl)-1,4-dihydroimidazo[4′,5′:4,5]pyrano[3,2-d]pyrimidin-8-amine

This compound was prepared according to the procedures described inExample (Step 1), Intermediate 1 (Step 1) and Example 20, using2H-pyran-3,5(4H,6H)-dione instead of 4,4-dimethylcyclohexane-1,3-dioneas starting material. LCMS calculated for C₁₇H₂₃N₆O₃S (M+H)⁺: m/z=391.2;Found 391.0.

Example 27.1-Isopropyl-2-methyl-N-(1-(methylsulfonyl)piperidin-4-yl)-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine

Step 1:3-Isopropyl-2-methyl-3,5,6,7-tetrahydro-4H-benzo[d]imidazol-4-one

This compound was prepared according to the procedures described inIntermediate 2, using 2-iodopropane instead of MeI (2 M in tert-BuOMe)as starting material. LCMS calculated for C₁₁H₁₇N₂O (M+H)⁺: m/z=193.1;Found 193.1.

Step 2:1-Isopropyl-2-methyl-N-(1-(methylsulfonyl)piperidin-4-yl)-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine

This compound was prepared according to the procedures described inExample 7, using3-isopropyl-2-methyl-3,5,6,7-tetrahydro-4H-benzo[d]imidazol-4-oneinstead of 2-methyl-3,5,6,7-tetrahydro-4H-benzo[d]imidazol-4-one asstarting material. LCMS calculated for C₁₉H₂₉N₆O₂S (M+H)⁺: m/z=405.2;Found 405.1.

Example 28.1,2-Dimethyl-N-((3R,4S)-3-methyl-1-((1-methyl-1H-imidazol-4-yl)sulfonyl)piperidin-4-yl)-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine

Step 1: tert-Butyl (3R,4S)-4-guanidino-3-methylpiperidine-1-carboxylatehydrochloride

This compound was prepared according to the procedures described inIntermediate 4, using tert-butyl(3R,4S)-4-amino-3-methylpiperidine-1-carboxylate instead of1-(methylsulfonyl)piperidin-4-amine as starting material. LCMScalculated for C₁₂H₂₅N₄O₂ (M−HCl+H)⁺: m/z=257.2; Found 257.2.

Step 2: tert-Butyl(3R,4S)-4-((1,2-dimethyl-4,5-dihydro-H-imidazo[4,5-h]quinazolin-8-yl)amino)-3-methylpiperidine-1-carboxylate

This compound was prepared according to the procedures described inIntermediate 3, using tert-butyl(3R,4S)-4-guanidino-3-methylpiperidine-1-carboxylate hydrochlorideinstead of tert-butyl 4-guanidinopiperidine-1-carboxylate hydrochlorideas starting material. LCMS calculated for C₂₂H₃₃N₆O₂ (M+H)⁺: m/z=413.3;Found 413.2.

Step 3:1,2-Dimethyl-N-((3R,4S)-3-methyl-1-((1-methyl-1H-imidazol-4-yl)sulfonyl)piperidin-4-yl)-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine

This compound was prepared according to the procedures described inExample 1, using tert-butyl(3R,4S)-4-((1,2-dimethyl-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-yl)amino)-3-methylpiperidine-1-carboxylateinstead of tert-butyl4-((1,2-dimethyl-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-yl)amino)piperidine-1-carboxylate,and 1-methyl-1H-imidazole-4-sulfonyl chloride instead of ethanesulfonylchloride as starting material. ¹H NMR (TFA salt, 500 MHz, DMSO-d₆) δ8.16 (brs, 1H), 7.82 (d, J=1.4 Hz, 1H), 7.79 (d, J=1.4 Hz, 1H), 7.61(brs, 1H), 4.06 (brs, 3H), 3.93 (m, 1H), 3.71 (s, 3H), 3.29 (m, 1H),3.17 (m, 1H), 3.02-2.79 (m, 6H), 2.58 (s, 3H), 2.20 (m, 1H), 1.84 (m,1H), 1.69 (m, 1H), 0.90 (d, J=6.9 Hz, 3H). LCMS calculated forC₂₁H₂₉N₈O₂S (M+H)⁺. m/z=457.2; Found 457.1.

Example 29.1,2-Dimethyl-N-((3R,4S)-3-methyl-1-((6-(4-methylpiperazin-1-yl)pyridin-2-yl)sulfonyl)piperidin-4-yl)-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine

Step 1:N-((3R,4S)-1-((6-Bromopyridin-2-yl)sulfonyl)-3-methylpiperidin-4-yl)-1,2-dimethyl-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine

This compound was prepared according to the procedures described inExample 28, using 6-bromopyridine-2-sulfonyl chloride instead of1-methyl-1H-imidazole-4-sulfonyl chloride as starting material. LCMScalculated for C₂₂H₂₇BrN₇O₂S (M+H)⁺: m/z=532.1; Found 532.2.

Step 2:1,2-Dimethyl-N-((3R,4S)-3-methyl-1-((6-(4-methylpiperazin-1-yl)pyridin-2-yl)sulfonyl)piperidin-4-yl)-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine

A solution ofN-((3R,4S)-1-((6-bromopyridin-2-yl)sulfonyl)-3-methylpiperidin-4-yl)-1,2-dimethyl-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amineand 1-methylpiperazine in DMF (1 mL) was heated at 100° C. for an hour.The resultant mixture was diluted with MeOH and purified by prep-LCMS(XBridge C18 column, eluting with a gradient of acetonitrile/watercontaining 0.1% NH₄OH, at flow rate of 60 mL/min) to afford the desiredproduct. LCMS calculated for C₂₇H₃N₉O₂S (M+H)⁺: m/z=552.3; Found 552.4.

Example 30.(R)-1-(5-(((3R,4S)-4-((1,2-Dimethyl-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-yl)amino)-3-methylpiperidin-1-yl)sulfonyl)pyridin-2-yl)pyrrolidin-3-ol

This compound was prepared according to the procedures described inExample 29, using 6-chloropyridine-3-sulfonyl chloride instead of6-bromopyridine-2-sulfonyl chloride, and (R)-pyrrolidin-3-ol instead of1-methylpiperazine as starting material. LCMS calculated for C₂₆H₃₅N₈O₃S(M+H)⁺: m/z=539.3; Found 539.4.

Example 31.1-Cyclopropyl-2-methyl-N-((3R,4S)-3-methyl-1-((1-methyl-1H-imidazol-4-yl)sulfonyl)piperidin-4-yl)-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine

This compound was prepared according to the procedures described inExample 28, using3-cyclopropyl-2-methyl-3,5,6,7-tetrahydro-4H-benzo[d]imidazol-4-oneinstead of 2,3-dimethyl-3,5,6,7-tetrahydro-4H-benzo[d]imidazol-4-one asstarting material. LCMS calculated for C₂₃H₃₁N₈O₂S (M+H)⁺: m/z=483.2;Found 483.2.

Example 32.1-Cyclopropyl-2-methyl-N-((3R,4S)-3-methyl-1-((1-methyl-1H-pyrazol-4-yl)sulfonyl)piperidin-4-yl)-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine

This compound was prepared according to the procedures described inExample 31, using 1-methyl-1H-pyrazole-4-sulfonyl chloride instead of1-methyl-1H-imidazole-4-sulfonyl chloride as starting material. LCMScalculated for C₂₃H₃₁N₈O₂S (M+H)⁺: m/z=483.2; Found 483.2.

Example A. CDK2/Cyclin E1 HTRF Enzyme Activity Assay

CDK2/Cyclin E1 enzyme activity assays utilize full-length human CDK2co-expressed as N-terminal GST-tagged protein with FLAG-Cyclin E1 in abaculovirus expression system (Carna Product Number 04-165). Assays areconducted in white 384-well polystyrene plates in a final reactionvolume of 8 μL. CDK2/Cyclin E1 (0.25 nM) is incubated with compounds (40nL serially diluted in DMSO) in the presence of ATP (50 μM or 1 mM) and50 nM ULight™-labeled eIF4E-binding protein 1 (THR37/46) peptide(PerkinElmer) in assay buffer (containing 50 mM HEPES pH 7.5, 1 mM EGTA,10 mM MgCl₂, 2 mM DTT, 0.05 mg/ml BSA, and 0.01% Tween 20) for 60minutes at room temperature. The reactions are stopped by the additionof EDTA and Europium-labeled anti-phospho-4E-BP1 antibody (PerkinElmer),for a final concentration of 15 mM and 1.5 nM, respectively. HTRFsignals are read after 1 hour at room temperature on a PHERAstar FSplate reader (BMG Labtech). Data is analyzed with IDBS XLFit andGraphPad Prism 5.0 software using a three or four parameter doseresponse curve to determine IC₅₀ for each compound. The IC₅₀ data asmeasured for the Examples at 1 mM ATP in the assay of Example A is shownin Table 1.

TABLE 1 Example IC₅₀ (nM) 1 + 2 + 3 + 4 + 5 + 6 + 7 ++ 8 + 9 ++ 10 +++11 + 12 + 13 + 14 + 15 + 16 + 17 + 18 + 19 + 20 + 21 + 22 + 23 + 24 +25 + 26 + 27 + 28 + 29 + 30 + 31 + 32 + + refers to ≤50 nM ++ refersto >50 nM to 200 nM +++ refers to >200 nM to 500 nM ++++ refers to >500nM to 1000 nM

Example B1. Characterization of Cyclin E1 in Ovarian and EndometrialCancer Cell Lines

The cyclin E1 (“CCNE1”) gene was evaluated in various ovarian andendometrial cancer cell lines (FIGS. 1A and 1). CCNE1 was amplified inCOV318, OVCAR3 OVARY, Fu-OV1, and KLE cells, each of which displayed aCCNE1 gain of function by copy number (copy number (“CN”) >2) (FIG. 1A).In contrast, CCNE1 was not amplified in COV504, OV56, or Igrov1 cells,each of which displayed copy neutral (2) or loss of function of the gene(CN≤2). CN was obtained from the Broad Institute Cancer Cell LineEncyclopedia (“CCLE”) database (Barretina, et al., Nature, 2012,483(7391):603-7, which is incorporated herein by reference in itsentirety).

Western blot analysis was performed on protein samples from COV318,OVCAR3_OVARY, Fu-OV1, KLE, COV504, OV56, and Igrov1 cells to evaluateCCNE1 protein levels. CCNE1 protein levels were higher in cell lineswith CCNE1 gain of function by copy number (CN >2; i.e., COV318, OVCAR3OVARY, Fu-OV1, and KLE cells) compared to cell lines with copy neutralor loss of function of the gene (CN≤2; i.e., COV504, OV56, and Igrov1cells).

Example B2. CDK2-Knockdown by siRNA Inhibits Proliferation inCCNE1-Amplified, but not CCNE1-Non-Amplified Human Cancer Cell Lines

The effect of CDK2-knockdown in CCNE1-amplified versusCCNE1-non-amplified cell lines was evaluated. CCNE1-amplified cell lines(Fu-OV1 and KLE) or CCNE1-non-amplified cell lines (COV504 and Igrov1)were treated with a control (“ctrl”) or CDK2-specific small interferingRNAs (“siRNAs”) (“CDK2 siRNA-1” and “CDK2 siRNA-2”) (FIGS. 2A and 2B and3A and 3B). Seventy-two hours after transfection with the siRNAs, thecells were harvested and subjected to cell cycle analysis byfluorescence activated cell sorting (“FACS”) (FIGS. 2A and 3A).Knockdown of CDK2 was confirmed by western blot (FIGS. 2B and 3B).CDK2-knockdown inhibited proliferation in CCNE1-amplified cell lines,but not in CCNE1-non-amplified cell lines (FIGS. 2A and 3A).

A similar experiment was performed in additional CCNE1-amplified celllines (COV318, OVCAR3, Fu-OV1, and KLE) and CCNE1-non-amplified celllines (COV504, OV56, and Igrov1) (FIG. 4). The percentage of cells atthe S phase three days after treatment with CDK2-specific siRNAs wassignificantly decreased in CCNE1-amplified cell lines as compared totreatment with control siRNA (FIG. 4). Consistent with the results ofFIGS. 2A and 3A, the percentage of cells at the S phase three days aftertreatment with CDK2-specific siRNAs was not significantly different inCCNE1-non-amplified cell lines as compared to treatment with controlsiRNA (FIG. 4).

Example B3. Proliferation in CCNE1 Amplified and CCNE-Non-Amplified CellLines Upon CDK4/6 Inhibition

The effect of CDK4/6-inhibition in CCNE1-amplified versusCCNE1-non-amplified cell lines was evaluated. CCNE1-amplified cells(OVCAR3) or CCNE1-non-amplified cells (COV504) were treated withdimethyl sulfoxide (“DMSO”) control or increasing concentrations ofCDK4/6 inhibitor palbociclib (FIG. 5). Sixteen hours after treatmentwith DMSO or palbociclib, the cells were harvested and subjected to cellcycle analysis by FACS (FIG. 5). CDK4/6-inhibition resulted indose-dependent inhibition of the proliferation in CCNE1-non-amplifiedcells, but not in CCNE1-amplified cells (FIG. 5).

A similar experiment was performed in a larger set of CCNE1-amplifiedcell lines (COV318 and OVCAR3) and CCNE1-non-amplified cell lines(COV504, OV56, and Igrov1) (FIG. 6). The percentage of cells at the Sphase 16 hours after treatment with palbociclib was decreased inCCNE1-non-amplified cell lines in a dose-dependent fashion as comparedto treatment with DMSO (FIG. 6). Consistent with the results of FIG. 5,the percentage of cells at the S phase 16 hours after treatment withpalbociclib was not significantly different in CCNE1-amplified celllines as compared to treatment with DMSO (FIG. 6).

Example B4. CDK2-Knockdown Blocks Rb Phosphorylation at S780 inCCNE1-Amplified, but not in CCNE1-Non-Amplified, Cell Lines

The effect of CDK2-knockdown on Rb phosphorylation at Ser-780 of SEQ IDNO:3 (“S780”) in CCNE1-amplified versus CCNE1-non-amplified cell lineswas evaluated. CCNE1-amplified cell lines (COV318, Fu-OV1 and KLE) orCCNE1-non-amplified cell lines (COV504, OV56 and Igrov1) were treatedwith ctrl or CDK2-specific siRNAs (FIGS. 7A and 7B). 72 hours aftertransfection with the siRNAs, the cells were harvested and total proteinwas extracted and analyzed by western blot. Knockdown of CDK2 wasconfirmed by western blot. CDK2-knockdown blocked Rb phosphorylation atS780 in CCNE1-amplified cell lines (FIG. 7A), but not inCCNE-non-amplified cell lines (FIG. 7B).

Example B5. Palbociclib Blocks Rb Phosphorylation at S780 in CCNE1Non-Amplified, but not in CCNE1-Amplified, Cell Lines

The effect of CDK4/6-inhibition on Rb phosphorylation at 5780 inCCNE1-amplified versus CCNE1-non-amplified cell lines was evaluated.CCNE1-amplified cell lines (OVCAR3 and COV318) or CCNE1-non-amplifiedcell lines (COV504 and OV56) were treated with DMSO or various doses ofpalbociclib (FIGS. 8A and 8B). One or 15 hours after treatment, thecells were harvested and total protein was extracted and analyzed bywestern blot (FIG. 8). Palbociclib treatment blocked Rb phosphorylationat 5780 in CCNE1-non-amplified cell lines (FIG. 8B), but not inCCNE1-amplified cell lines (FIG. 8A).

Example B6. CDK2 Degradation by dTAG Decreases Rb Phosphorylation atS780

To further confirm that CDK2 knockdown decreases Rb phosphorylation atS780 in CCNE1-amplified cells (see Example B4), the dTAG system was usedto degrade CDK2 and the level of 5780-phosphorylated Rb was evaluated(Erb et al., Nature, 2017, 543(7644):270-274, which is incorporatedherein by reference in its entirety). Briefly, OVCAR3 cells wereengineered to express Cas9 by lentiviral transduction of Cas9 construct.The OVCAR3-Cas9 cells were then engineered to express CDK2-FKBP12F36V-HAfusion protein by lentiviral transduction of CDK2-FKBP12F36V-HAexpression construct. Next, to engineer the line to have endogenous CDK2inactivated, OVCAR3 (Cas9, CDK2-FKBP12F36V-HA) cells were transducedwith CDK2 sgRNA (“CDK2-gRNA”); OVCAR3 (Cas9, CDK2-FKBP12F36V-HA) cellstransduced with non-targeting sgRNA (“Ctl-gRNA”; Cellecta) served as acontrol cell line.

To degrade CDK2-FKBP12F36V-HA protein by dTAG (FIG. 9A), cells weretreated with DMSO or with a titration of concentrations of dTAG for 14hours. Cells were collected and processed for Western blot (FIG. 9B). Adose-responsive degradation of CDK2-FKBP12(F36V) was detected by westernblot after treatment with dTAG in both control- and CDK2-gRNA treatedcells (FIG. 9B). Degradation was further confirmed by western blot forHA-Tag. Endogenous CDK2 protein was detected in OVCAR3 cells treatedwith control gRNA, but not with CDK2-gRNA (FIG. 9B). CDK2-FKBP12(F36V)degradation inhibited Rb phosphorylation at S780 in CDK2 knockout OVCAR3cells, but not in OVCAR3 cells with endogenous CDK2 expression.

Example B7. p-Rb S780 HTRF Cellular Assay for Identification of CDK2Inhibitors

An in vitro CDK2/CCNE1 enzyme activity assay was used to measurephosphorylation of a peptide substrate using homogenous time-resolvedenergy transfer (“HTRF”). First, the specificity of8-((1R,2R)-2-hydroxy-2-methylcyclopentyl)-2-((1-(methylsulfonyl)piperidin-4-yl)amino)pyrido[2,3-d]pyrimidin-7(8H)-one(Compound A; see US Patent Application Publication No. 2018/0044344 atpage 51, paragraph [0987], which is incorporated by reference herein inits entirety) for CDK2 inhibition was confirmed via a kinase activityassay (FIG. 10A). To this end, the LANCE® Ultra kinase assay was usedwith a ULight™-labeled EIF4E-binding protein 1 (Thr37/46) peptide(PerkinElmer, TRF0128-M) as substrate and an Europium-labeledanti-phospho-EIF4E binding protein1 (Thr37/46) antibody (PerkinElmer,TRF0216-M). A ratio of fluorescence transferred to the labeled substrate(665 nm) relative to fluorescence of the Europium donor (620 nm)represents the extent of phosphorylation. The IC₅₀ for Compound A wasdetermined to be 1.1 nM (FIG. 10A). In contrast, the IC₅₀ for the CDK4/6inhibitor palbociclib was 10,000 nM (FIG. 10A).

Next, a CDK2 pRb (S780) HTRF cellular assay was performed, enabling thequantitative detection of Rb phosphorylated on serine 780 in CCNE1amplified COV318 cells upon treatment with Compound A or palbociclib(FIG. 10B). Treatment with Compound A, but not palbociclib, inhibited Rbphosphorylation on serine 780 in CCNE1 amplified cells (FIG. 10B). TheIC₅₀ for Compound A in this assay was 37 nM, while the IC₅₀ forpalbociclib was >3,000 nM (FIG. 10B).

Example B8. Bioinformatics Analysis of CCLE Dataset Reveals theSensitivity to CDK2 Inhibition in CCNE1 Amplified Cells Relies onFunctional p16

In an attempt to identify a biomarker for predicting sensitivity toCDK2-inhibition in CCNE1-amplified cells, 460 cell lines from CCLE wereanalyzed (Barretina, supra). First, the cell lines were filtered basedon CCNE1 copy number and expression and CDK2 sensitive score based onshRNA knockdown data. A total of 41 cell lines were identified as havingCCNE1 copy number of >3 and CCNE1 expression score (CCLE: >3). Of these41 cell lines, 18 (44%) were sensitive to CDK2 inhibition (CDK2sensitive score ≤−3), while 23 (56%) were insensitive to CDK2 inhibition(CDK2 sensitive score >−3).

Next, the p16 status was evaluated in the CDK2-sensitive andCDK2-insensitive cell lines (FIG. 11). Of the 18 cell lines that weresensitive to CDK2-inhibition, 100% expressed normal p16 gene (FIG. 11).In contrast, only 4 of the 23 CDK2-insensitive cell lines expressednormal p16 gene (FIG. 11). The majority of the 23 CDK2-insensitive celllines displayed dysfunctional p16 gene expression: the p16 gene wasdeleted in 10 of 23 cell lines; the p16 gene was silenced in 5 of the 23cell lines, and the p16 gene was mutated in 4 of the 23 cell lines (FIG.11).

A summary of CDK2 sensitivity and CDKN2A/p16 status in CCNE1 amplifiedcell lines is provided in Table 2, below.

TABLE 2 Cell lines with CDK2 sensitive Score ≤3 counted as CDK2Sensitive lines; ≥3 as CDK2 insensitive line. Cell lines verified inexperiments are in bold. NCIN87_STOMACH showed no CDKN2A/P16 proteinexpression in western blot. CCNE1 and CDKN2A/P16 copy number werecalculated based on CCLE dataset. Expression Score <0 counted as genesilencing. CDKN2A/ p16 CDK2 CCNE1 CDKN2A mRNA CDKN2a/ sensitive CopyCopy Expression p16 Cell Lines Score No. No. Score Dysfunction HCC1569_(—) BREAST −9.6 16 2 5.11 OVISE_OVARY −9.4 3 2 4.17 MKN1 _(—) STOMACH−8.9 5 1 4.28 EFE184_ENDOMETRIUM −8.7 3 2 3.97 KURAMOCHI_OVARY −8.2 3 23.60 MKN7 _(—) STOMACH −7.7 21 1 4.37 MDAMB157_BREAST −7.6 6 2 5.01HCC70_BREAST −7.6 4 4 4.88 NIHOVCAR3 _(—) OVARY −7.4 10 2 4.15 FUOV1_(—) OVARY −7 10 3 5.19 KLE _(—) ENDOMETRIUM −7 7 2 6.24 COV318 _(—)OVARY −7 14 2 5.09 CAOV4_OVARY −6.7 3 2 3.59 MFE280_ENDOMETRIUM −6.3 4 24.97 NCIH661_LUNG −6.2 5 2 3.73 OVCAR4_OVARY −4.3 4 1 4.77 SNU8_OVARY−3.8 5 3 5.35 OVCAR8_OVARY −3.7 3 2 5.21 RMUGS_OVARY −2.8 4 1 −0.08Silencing NCCSTCK140_STOMACH −2.7 3 0 −4.70 Deletion NCIH2286_LUNG −1.63 1 3.63 Mutation HOP62_LUNG −1.4 4 0 −1.21 DeletionLN340_CENTRAL_NERVOUS_SYSTEM −1.0 3 0 −5.47 Deletion NCIH1339_LUNG −0.83 2 2.42 Unknown NCIN87 _(—) STOMACH 0.1 3 2 4.67 No protein U2OS_BONE0.4 3 1 −5.72 Silencing SF172_CENTRAL_NERVOUS_SYSTEM 0.5 3 0 −2.35Deletion CAL120_BREAST 0.6 4 1 4.86 RMGI_OVARY 0.9 3 0 −3.33 DeletionOV90_OVARY 0.9 3 1 3.95 Mutation SNU601_STOMACH 1.1 4 2 −3.79 SilencingEW8_BONE 1.5 5 1 3.11 JHESOAD1_OESOPHAGUS 1.7 5 0 −5.52 DeletionHCC1806_BREAST 1.9 8 0 −4.61 Deletion NCIH2170_LUNG 2.0 3 0 −3.73Deletion HCC1428_BREAST 2.3 3 2 2.28 A549_LUNG 2.5 4 0 −6.13 DeletionLXF289_LUNG 2.6 4 3 4.10 Mutation AGS _(—) STOMACH 3.0 3 2 −5.56Silencing NCIH647_LUNG 3.0 4 0 −5.07 Deletion HLF_LIVER 3.9 3 2 3.40

Example B9. CCNE1 Amplified Cells with Dysfunctional p16 do not Respondto CDK2 Inhibition

To further evaluate the role of p16 in CDK2-sensitivity inCCNE-amplified cells, p16 protein expression in three gastric cell lineswith CCNE1-amplification was evaluated by western blot. AGS and NCI-N87cells displayed absent or dramatically reduced levels of p16 (FIG. 12A).In contrast, p16 protein was detected in MKN1 cellular protein extracts(FIG. 12A).

Next, the impact of CDK2-knockdown in these cells was evaluated. Mkn1,Ags, and NCI-N87 cells were treated with control or CDK2-specific siRNA.Three days-post-siRNA transfection, cell cycle phase distribution of thecells was evaluated by FACS.

The percentage of cells at the S phase in the Mkn1 cells(CCNE-amplified, p16 protein detected) was significantly decreased inthe CDK2 siRNA-treated cells as compared to control (FIG. 12B). Incontrast, the percentage of cells at the S phase was not significantlydecreased in Ags and NCI-N87 cells (CCNE1-amplified, dysfunctional p16protein levels) after treatment with CDK2 siRNA as compared to control(FIG. 12B).

Example B10. p16 Knockdown by siRNA Abolishes CDK2 Inhibition InducedCell Cycle Suppression in CCNE1 Amplified Cells

To confirm the role of p16 in CDK2-sensitivity of CCNE-amplified cells,COV318 cells were treated with control or p16-specific siRNA.Seventy-two hours after transfection, cells were treated with DMSO(control) or 100 nM of Compound A. Sixteen hours after treatment withDMSO or the CDK2-inhibitor, cells were harvested and subjected to cellcycle analysis by FACS. Consistent with the results described above, thepercentage of S phase cells significantly decreased in the controlsiRNA-treated cells treated with CDK2-inhibitor (Compound A), but notwith the DMSO control (FIG. 13). In contrast, the percentage of S phasecells was not significantly decreased after treatment with theCDK2-inhibitor (Compound A) in p16 knocked down cells as compared toDMSO control (FIG. 13).

Materials and Methods Used in Examples B1-B10

Cell Culture and Transfection

Human cyclin E1 (CCNE1) amplified ovarian cell lines OVCAR3, COV318,Fu-OV1, endometrial cell line KLE, gastric cell lines MKN1, AGS, NCIN87,and CCNE1 non-amplified ovarian cell lines COV504, OV56, Igrov1 werecultured in RPMI 1640 medium. The complete growth medium wassupplemented with 10% FBS, 0.1 mM non-essential amino acids, 2 mML-glutamine, 100 units/mL penicillin G and 100 μg/mL streptomycin in 37°C. humidified incubator and an atmosphere of 5% CO₂ in air. Fu-OV1 linewas purchased from Leibniz-Institute DSMZ—German Collection ofMicroorganisms and Cell Cultures; MKN1 was purchased from JapaneseCancer Research Resources Bank; and the rest of cell lines werepurchased from American Type Culture Collection. For transfection, cellswere seeded into 6-well for 24 hours and transiently transfected byLipofectamine 2000 Reagent (Thermo Fisher, 11668027). ON-TARGETplusHuman CKD2 siRNAs (GE Healthcare Dharmacon, J-003236-11-0002 andJ-003236-12-0002) and ON-TARGETplus Human CDKN2A/p16 siRNAs (GEHealthcare Dharmacon, J-011007-08-0002) were used to knockdown theendogenous CDK2 and CDKN2A/p16. ON-TARGETplus Non-targeting Pool (GEHealthcare Dharmacon, D-001810-10-20) was used as a negative control.

Western Blot Analysis

Whole cell extracts were prepared using RIPA buffer (Thermo Scientific,89900) with a Halt Protease and Phosphatase Inhibitor Cocktail (ThermoScientific, 78440). Protein concentration was quantified with a BCAProtein Assay Kit (Thermo Scientific, 23225) and 40 g of protein lysateswere loaded for SDS-PAGE using precast gradient gels (Bio-Rad, Hercules,No. 456-1094). Samples were diluted in 5× Laemmli buffer (300 mMTris-HCl pH 6.8, 10% SDS (w/v), 5% 2-mercaptoethanol, 25% glycerol(v/v), 0.1% bromophenol blue w/v) and boiled for 5 minutes. 35 g ofproteins were separated by 8-15% SDS-PAGE and transferred ontopolyvinylidene fluoride (PVDF) membranes. Unspecific binding sites onthe PVDF membranes were blocked with 5% non-fat milk in TBST (20 mMTris-HCl, pH 7.6, 137 mM NaCl, 1% Tween-20). Membranes were hybridizedwith antibodies against anti-CDKN2A/p16 (Cell Signaling Technology,92803S), anti-Cas9 (Cell Signaling Technology, 97982S), anti-HA (CellSignaling Technology, 3724S), anti-Rb (Cell Signaling Technology,9309S), anti-phospho-Rb (Ser780) (Cell Signaling Technology, 8180S),anti-CDK2 (Cell Signaling Technology, 2546S), anti-CCNE1 (Cell SignalingTechnology, 20808S) and anti-GAPDH (Cell Signaling Technology, 8884S)for overnight at 4° C., followed by incubation with horseradishperoxidase (HRP)-conjugated secondary antibodies for 1 hour at roomtemperature. The membranes were then developed using Immobilon Westernchemiluminescence HRP substrates (Millipore, WBKLS0500). Images werecaptured by Luminescence/Fluorescence Imaging System Odyssey CLx Imager(LI-COR).

Cell Cycle Analysis

Cells were seeded in six-well tissue culture plates and 24 hours laterwere treated with a titration of concentrations of Palbociclib orCompound A. After overnight treatment, cells exposed to 10 M EdU for 3hours before detection of EdU-DNA by Click-iT AlexaFluor® 647 azide kit(Life Technology, C10424) following the manufacturer's instructions.Bulk DNA was stained with DAPI. Compound-treated and DMSO treatedcontrol cells were acquired with CytoFlex (Beckman Coulter) and wereanalyzed using the FlowJo software. For cell cycle analysis of cellswith siRNA knockdown, 72 hours after siRNA transfection, cells exposedto 10 M EdU for 3 hours before detection of Click-iT Alexa Fluor® 647azide kit.

Plasmids

LentiCas9 plasmid pRCCH-CMV-Cas9-2A (Cellecta, SVC9-PS) was used forCas9 expression. sgRNA-CDK2 lentiviral construct, designed to targetAAGCAGAGATCTCTCGGA (SEQ ID NO:8) of CDK2, was cloned into sgRNAexpression vector pRSG-U6 and purchased from Cellecta (93661). ForCDK2-FKBP12F36V-HA expression, a 1306 base pair DNA fragment encodingCDK2 and FKBP12F36V-2xHA tag at the C-terminus was synthesized andcloned into EcoRI and BamHI digested pCDH-EF1α-MCS-T2A-Puro lentivector(Systembio, CD527A-1).

Sequence of 1306 bp DNA fragment: (SEQ ID NO: 4)CCTCGAATTCAGCTGCATGGAGAACTTCCAAAAGGTGGAAAAGATCGGAGAGGGCACGTACGGAGTTGTGTACAAAGCCAGAAACAAGTTGACGGGAGAGGTGGTGGCGCTTAAGAAAATCCGCCTGGACACTGAGACTGAGGGTGTGCCCAGTACTGCCATCCGAGAGATCTCTCTGCTTAAGGAGCTTAACCATCCTAATATTGTCAAGCTGCTGGATGTCATTCACACAGAAAATAAACTCTACCTGGTTTTTGAATTTCTGCACCAAGATCTCAAGAAATTCATGGATGCCTCTGCTCTCACTGGCATTCCTCTTCCCCTCATCAAGAGCTATCTGTTCCAGCTGCTCCAGGGCCTAGCTTTCTGCCATTCTCATCGGGTCCTCCACCGAGACCTTAAACCTCAGAATCTGCTTATTAACACAGAGGGGGCCATCAAGCTAGCAGACTTTGGACTAGCCAGAGCTTTTGGAGT A CCTGTTCGTACTTACACCCA TGA AGTGGTGACCCTGTGGTACCGAGCTCCTGAAATCCTCCTGGGCTGCAAATATTATTCCACAGCTGTGGACATCTGGAGCCTGGGCTGCATCTTTGCTGAGATGGTGACTCGCCGGGCCCTATTCCCTGGAGATTCTGAGATTGACC AGCTCTT TCGGATCTTTCGGACTCTGGGGACCCCAGATGAGGTGGTGTGGCCAGGAGTTACTTCTATGCCTGATTACAAGCCAAGTTTCCCCAAGTGGGCCCGGCAAGATTTTAGTAAAGTTGTACCTCCCCTGGATGAAGATGGACGGAGCTTGTTATCGCAAATGCTGCACTACGACCCTAACAAGCGGATTTCGGCCAAGGCAGCCCTGGCTCACCCTTTCTTCCAGGATGTGACCAAGCCAGTAC CCCATCTTCGACTCGGAGTGCAGGTGGAAACCATCTCCCCAGGAGACGGGCGCACCTTCCCCAAGCGCGGCCAGACCTGCGTGGTGCACTACACCGGGATGCTTGAAGATGGAAAGAAAGTTGATTCCTCCCGGGACAGAAACAAGCCCTTTAAGTTTATGCTAGGCAAGCAGGAGGTGATCCGAGGCTGGGAAGAAGGGGTTGCCCAGATGAGTGTGGGTCAGAGAGCCAAACTGACTATATCTCCAGATTATGCCTATGGTGCCACTGGGCACCCAGGCATCATCCCACCACATGCCACTCTCGTCTTCGATGTGGAGCTTCTAAAACTGGAAGGATACCCTTACGACGTTCCTGATTACGCTTACCCTTACGACGTTCCTGATTACGCT GGATCCTA ATTCGAAAGC

GAATTC (SEQ ID NO:5; EcoRI), GGATCC (SEQ ID NO:6; BamHI) and TTCGAA (SEQID NO:7; BstBI) restriction enzyme sites were underlined. Sequenceencoding CDK2 is in bold and sequence of FKBP12F36V-HA is in italics.Three nucleic acids underlined within the CDK2 sequence indicatedmodifications that abolished PAM sites to avoided CRISPR knockouteffect. These changes did not change amino acids encoded.

Lentivirus Production

Production of lentivirus was performed in 293T cells by co-transfectionof Lentiviral Packaging Mix (Sigma, SHP001), and a given lentiviralexpression plasmid using Lipofectamine 2000. Viral supernatants werecollected 48 and 72 hours after transfection, filtered through a 0.22 μmmembrane. All cells lines were transduced by spinoculation at 2000revolutions per minute (rpm) for 1 hour at room temperature with 8 μg/mLpolybrene (Santa Cruz, sc-134220).

CDK2-dTAG Cells

OVCAR3 cells were first engineered to express Cas9 by lentiviraltransduction of Cas9 construct. Cells were selected and maintained in100 μg/mL hygromycin (Life Technologies, 10687010) and verified toexpress Cas9 by immunoblot. OVCAR3-Cas9 cells were then engineered toexpress CDK2-FKBP12F36V-HA fusion protein by lentiviral transduction ofCDK2-FKBP12F36V-HA expression construct and selection with 2 μg/mLpuromycin dihydrochloride (Life Technologies, A1113803). Expression ofCDK2-FKBP12F36V-HA was verified by immunoblot using anti-CDK2 andanti-HA antibodies. Next, to engineer the line to have endogenous CDK2inactivated, OVCAR3 (Cas9, CDK2-FKBP12F36V-HA) cells were transducedwith CDK2 sgRNA and selected by 50 μg/mL Zeocin (Life Technologies,R25001). Inactivated expression of endogenous CDK2 in the expandedclones was tested by immunoblotting. OVCAR3 (Cas9, CDK2-FKBP12F36V-HA)cells transduced with non-targeting sgRNA (Cellecta) were served as acontrol cell line.

To degrade CDK2-FKBP12F36V-HA protein by dTAG, 200,000 cells were seededin 1 mL media in triplicate in a 24-well plate and treated with dimethylsulfoxide (DMSO) or with a titration of concentrations of dTAG for 14hours. Cells were collected and processed for Western blot.

CDK2 CCNE1 Enzymatic Assay

In vitro CDK2/CCNE1 enzyme activity assay measures phosphorylation of apeptide substrate using homogeneous time-resolved energy transfer(HTRF). The LANCE® Ultra kinase assay used a ULight™-labeledEIF4E-binding protein 1 (Thr37/46) peptide (PerkinElmer, TRF0128-M) assubstrate and an Europium-labeled anti-phospho-EIF4E binding protein1(Thr37/46) antibody (PerkinElmer, TRF0216-M). A ratio of fluorescencetransferred to the labeled substrate (665 nm) relative to fluorescenceof the Europium donor (620 nm) represents the extent of phosphorylation.Ratios for treated wells are normalized to DMSO only (100% activity) andno enzyme (0% activity) controls. Normalized data is analyzed using afour parameter dose response curve to determine IC₅₀ for each compound.

CDK2 pRb (S780) HTRF Cellular Assay

CDK2 pRb (S780) HTRF cellular assay enables the quantitative detectionof Rb phosphorylated on serine 780 in CCNE1 amplified COV318 cells. Theassay comprised two antibodies: Europium cryptate labeledanti-Phospho-Rb S780 antibody (donor) and d2 labeled anti-Rb antibody(acceptor). In brief, COV318 cells were seeded into the wells of 96-wellplate at a density of 25,000 per well with 9-point, 3-fold serialdiluted compounds and cultured overnight at 37 degree with 5% CO₂. Thefinal concentrations of compounds start from 3 μM. The next day cellswere lysed in 70 μL 1× Phospho-total protein lysis buffer #2 (Cisbio),supplemented with 0.7 μL blocking buffer (Cisbio) and 1.4 μL proteaseinhibitor cocktail set III, EDTA-free (Calbiochem, 539134). 16 μL ofcell lysates were mixed with 4 μL of the fluorophore-conjugatedantibodies to a final concentration of 0.188 nM cryptate-labeledanti-Phospho-Rb S780 antibody and 0.14 nM d2 labeled anti-Rb antibody.After 2 h of incubation at room temperature, HTRF signals were measuredon the PHERAstar microplate reader (BMG Labtech), using 340 nm asexcitation wavelength, a 620 nm filter for the Europium donorfluorescence, and a 665-nm filter for the acceptor fluorescencedetection. HTRF signals were calculated as the HTRF ratio (ratio offluorescence measured at 665 nm and 620 nm)×10000.

Various modifications of the invention, in addition to those describedherein, will be apparent to those skilled in the art from the foregoingdescription. Such modifications are also intended to fall within thescope of the appended claims. Each reference, including all patent,patent applications, and publications, cited in the present applicationis incorporated herein by reference in its entirety.

What is claimed is:
 1. A compound of Formula (I):

or a pharmaceutically acceptable salt thereof, wherein: n is 0, 1, 2, 3,4, 5, or 6; Z¹ is CR⁴R⁵; and Z² is NR^(6z), O, or S; or Z¹ is CR⁴R⁵,NR⁶, O, or S; and Z² is CR^(4z)R^(5z); Y is CR⁷ or N; X¹ is NR^(1x),CR¹, N, or O; X² is NR^(2x), CR², N, or O; X³ is N or C; wherein Ring

is an aromatic ring selected from:

Ring moiety A is selected from (1) C₃₋₁₄ cycloalkyl, wherein 1, 2, 3, or4 ring-forming carbon atoms of said C₃₋₁₄ cycloalkyl can be optionallysubstituted by one or more oxo or sulfido, and (2) 4-14 memberedheterocycloalkyl having 1, 2, 3, or 4 ring-forming heteroatomsindependently selected from N, O, and S, wherein 1, 2, 3, or 4ring-forming carbon or heteroatoms can be optionally substituted by oneor more oxo or sulfido; wherein Ring moiety A is attached to the —NH—group of Formula (I) at a ring member of a saturated or partiallysaturated ring of said C₃₋₁₄ cycloalkyl or said 4-14 memberedheterocycloalkyl; R¹ is selected from H, D, halo, CN, NO₂, C₁₋₆ alkyl,C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₁₋₆ haloalkyl, C₃₋₁₀ cycloalkyl, 6-10membered aryl, 4-10 membered heterocycloalkyl, 5-10 membered heteroaryl,C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10membered heterocycloalkyl-C₁₋₄ alkyl, 5-10 membered heteroaryl-C₁₋₄alkyl, OR^(a1), SR^(a1), NHOR^(a1), C(O)R^(b1), C(O)NR^(c1)R^(d1),C(O)NR^(c1)(OR^(a1)), C(O)OR^(a1), OC(O)R^(b1), OC(O)NR^(c1)R^(d1),NR^(c1)R^(d1), NR^(c1)NR^(d1), NR^(c1)C(O)R^(b1), NR^(c1)C(O)OR^(a1),NR^(c1)C(O)NR^(c1)R^(d1), C(═NR^(e1))R^(b1), C(═NR^(e8))NR^(c1)R^(d1),NR^(c1)C(═NR^(e1))NR^(c1)R^(d1), NR^(c1)C(═NR^(e1))R^(b1),NR^(c1)S(O)NR^(c1)R^(d1), NR^(c1)S(O)R^(b1), NR^(c1)S(O)₂R^(b1),NR^(c1)S(O)(═NR^(e1))R^(b1), NR^(c1)S(O)₂NR^(c1)R^(d1), S(O)R^(b1),S(O)NR^(c1)R^(d1), S(O)₂R^(b1), S(O)₂NR^(c1)R^(d1),OS(O)(═NR^(e1))R^(b1), OS(O)₂R^(b1), S(O)(═NR^(e1))R^(b1), SF₅,P(O)R^(f1)R^(g1), OP(O)(OR^(h1))(OR^(i1)), P(O)(OR^(h1))(OR^(i1)), andBR^(j1)R^(k1); wherein said C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₁₋₆haloalkyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10 memberedheterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl,6-10 membered aryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl are each optionallysubstituted by 1, 2, 3, or 4 independently selected R^(1A) substituents;R^(1x) is selected from H, C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₁₋₆haloalkyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10 memberedheterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl,6-10 membered aryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄alkyl, 5-10 membered heteroaryl-C₁₋₄ alkyl, C(O)R^(b1),C(O)NR^(c1)R^(d1), C(O)NR^(c1)(OR^(a1)), C(O)OR^(a1), C(═NR^(e1))R^(b1),C(═NR^(e1))NR^(c1)R^(d1), S(O)₂R^(b1), and S(O)₂NR^(c1)R^(d1); whereinsaid C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₁₋₆ haloalkyl, C₃₋₁₀cycloalkyl, 6-10 membered aryl, 4-10 membered heterocycloalkyl, 5-10membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 memberedaryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-10membered heteroaryl-C₁₋₄ alkyl are each optionally substituted by 1, 2,3, or 4 independently selected R^(1A) substituents; R² is selected fromH, D, halo, NO₂, CN, C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₁₋₆haloalkyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10 memberedheterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl,6-10 membered aryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄alkyl, 5-10 membered heteroaryl-C₁₋₄ alkyl, OR^(a2), SR^(a2), NHOR^(a2),C(O)R^(b2), C(O)NR^(c2)R^(d2), C(O)NR^(c2)(OR^(a2)), C(O)OR^(a2),OC(O)R^(b2), OC(O)NR^(c2)R^(d2), NR^(c2)R^(d2), NR^(c2)NR^(c2)R^(d2),NR^(c2)C(O)R^(b2), NR^(c2)C(O)OR^(a2), NR^(c2)C(O)NR^(c2)R^(d2),C(═NR^(e2))R^(b2), C(═NR^(e2))NR^(c2)R^(d2),NR^(c2)C(═NR^(e2))NR^(c2)R^(d2), NR^(c2)C(═NR^(e2))R^(b2),NR^(c2)S(O)NR^(c2)R^(d2), NR^(c2)S(O)R^(b2), NR^(c2)S(O)₂R^(b2),NR^(c2)S(O)(═NR^(e2))R^(b2), NR^(c2)S(O)₂NR^(c2)R^(d2), S(O)R^(b2),S(O)NR^(c2)R^(d2), S(O)₂R^(b2), S(O)₂NR^(c2)R^(d2),OS(O)(═NR^(e2))R^(b2), OS(O)₂R^(b2), S(O)(═NR^(e2))R^(b2), SF₅,P(O)R^(f2)R^(g2), OP(O)(OR^(h2))(OR^(i2)), P(O)(OR^(h2))(OR^(i2)), andBR^(j2)R^(k2); wherein said C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₁₋₆haloalkyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10 memberedheterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl,6-10 membered aryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl are each optionallysubstituted by 1, 2, 3, or 4 independently selected R^(2A) substituents;R^(2x) is selected from H, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₂₋₆ alkenyl,C₂₋₆ alkynyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10 memberedheterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl,6-10 membered aryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄alkyl, 5-10 membered heteroaryl-C₁₋₄ alkyl, C(O)R^(b2),C(O)NR^(c2)R^(d2), C(O)NR^(c2)(OR^(a2)), C(O)OR^(a2), C(═NR^(e2))R^(b2),C(═NR^(e2))NR^(c2)R^(d2), S(O)₂R^(b2), and S(O)₂NR^(c2)R^(d2); whereinsaid C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀cycloalkyl, 6-10 membered aryl, 4-10 membered heterocycloalkyl, 5-10membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 memberedaryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-10membered heteroaryl-C₁₋₄ alkyl are each optionally substituted by 1, 2,3, or 4 independently selected R^(2A) substituents; R³ is selected fromH, D, halo, CN, C₁₋₄ alkyl, C₁₋₄ haloalkyl, C₂₋₄ alkenyl, C₂₋₄ alkynyl,OH, C₁₋₃ alkoxy, C₁₋₃ haloalkoxy, amino, C₁₋₃ alkylamino, di(C₁₋₃alkyl)amino, and C₃₋₄ cycloalkyl; R⁴, R^(4z), R⁵ and R^(5z) areindependently selected from H, D, halo, CN, C₁₋₄ alkyl, C₁₋₄ haloalkyl,C₂₋₄ alkenyl, C₂₋₄ alkynyl, OH, C₁₋₃ alkoxy, C₁₋₃ haloalkoxy, amino,C₁₋₃ alkylamino, di(C₁₋₃ alkyl)amino, cyano-C₁₋₃ alkyl, HO—C₁₋₃ alkyl,C₁₋₃ alkoxy-C₁₋₃ alkyl, and C₃₋₄ cycloalkyl; or R⁴ and R⁵, together withthe carbon atom to which they are attached, form a 3-7 memberedcycloalkyl ring or a 4-7 membered heterocycloalkyl ring, which are eachoptionally substituted by 1, 2, 3, or 4 substituents independentlyselected from D, halo, CN, C₁₋₃ alkyl, C₁₋₃ haloalkyl, OH, C₁₋₃ alkoxy,C₁₋₃ haloalkoxy, amino, C₁₋₃ alkylamino, di(C₁₋₃ alkyl)amino, cyano-C₁₋₃alkyl, HO—C₁₋₃ alkyl, and C₁₋₃ alkoxy-C₁₋₃ alkyl; or R^(4z) and R^(5z),together with the carbon atom to which they are attached, form a 3-7membered cycloalkyl ring or a 4-7 membered heterocycloalkyl ring, whichare each optionally substituted by 1, 2, 3, or 4 substituentsindependently selected from D, halo, CN, C₁₋₃ alkyl, C₁₋₃ haloalkyl, OH,C₁₋₃ alkoxy, C₁₋₃ haloalkoxy, amino, C₁₋₃ alkylamino, di(C₁₋₃alkyl)amino, cyano-C₁₋₃ alkyl, HO—C₁₋₃ alkyl, and C₁₋₃ alkoxy-C₁₋₃alkyl; R⁶ and R^(6z) are each independently selected from H, C₁₋₄ alkyl,C₁₋₄ haloalkyl, C₂₋₄ alkenyl, C₂₋₄ alkynyl, cyano-C₁₋₃ alkyl, HO—C₁₋₃alkyl, C₁₋₃ alkoxy-C₁₋₃ alkyl, and C₃₋₄ cycloalkyl; R⁷ is selected fromH, D, halo, CN, C₁₋₄ alkyl, C₁₋₄ haloalkyl, C₂₋₄ alkenyl, C₂₋₄ alkynyl,OH, C₁₋₃ alkoxy, C₁₋₃ haloalkoxy, amino, C₁₋₃ alkylamino, di(C₁₋₃alkyl)amino, cyano-C₁₋₃ alkyl, HO—C₁₋₃ alkyl, C₁₋₃ alkoxy-C₁₋₃ alkyl,and C₃₋₄ cycloalkyl; each R⁸ is selected from D, halo, CN, NO₂, C₁₋₆alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₁₋₆ haloalkyl, C₃₋₁₀ cycloalkyl,6-10 membered aryl, 4-10 membered heterocycloalkyl, 5-10 memberedheteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl,4-10 membered heterocycloalkyl-C₁₋₄ alkyl, 5-10 membered heteroaryl-C₁₋₄alkyl, OR^(a8), SR^(a8), NHOR^(a8), C(O)R^(b8), C(O)NR^(c8)R^(d8),C(O)NR^(c8)(OR^(a8)), C(O)OR^(a8), OC(O)R^(b8), OC(O)NR^(c8)R^(d8),NR^(c8)R^(d8), NR^(c8)NR^(c8)R^(d8), NR^(c8)C(O)R^(b8),NR^(c8)C(O)OR^(a8), NR^(c8)C(O)NR^(c8)R^(d8), C(═NR^(e8))R^(b8),C(═NR^(e8))NR^(c8)R^(d8), NR^(c8)C(═NR^(e8))NR^(c8)R^(d8),NR^(c8)C(═NR^(e8))R^(b8), NR^(c8)S(O)NR^(c8)R^(d8), NR^(c8)S(O)R^(b8),NR^(c8)S(O)₂R^(b8), NR^(c8)S(O)(═NR^(e8))R^(b8),NR^(c8)S(O)₂NR^(c8)R^(d8), S(O)R^(b8), S(O)NR^(c8)R^(d8), S(O)₂R^(b8),S(O)₂NR^(c8)R^(d8), OS(O)(═NR^(e8))R^(b8), OS(O)₂R^(b8),S(O)(═NR^(e8))R^(b8), SF₅, P(O)R^(f8)R^(g8), OP(O)(OR^(h8))(OR^(i8)),P(O)(OR^(h8))(OR^(i8)), and BR^(j8)R^(k8); wherein said C₁₋₆ alkyl, C₂₋₆alkenyl, C₂₋₆ alkynyl, C₁₋₆ haloalkyl, C₃₋₁₀ cycloalkyl, 6-10 memberedaryl, 4-10 membered heterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted by 1, 2, 3, or 4 independently selectedR^(8A) substituents; each R^(1A) is independently selected from D, halo,CN, NO₂, C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₁₋₆ haloalkyl, C₃₋₁₀cycloalkyl, 6-10 membered aryl, 4-10 membered heterocycloalkyl, 5-10membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 memberedaryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄ alkyl, 5-10membered heteroaryl-C₁₋₄ alkyl, OR^(a11), SR^(a11), NHOR^(a11),C(O)R^(b11), C(O)NR^(c11)R^(d11), C(O)NR^(c11)(OR^(a11)), C(O)OR^(d11),OC(O)R^(b11), OC(O)NR^(c11)R^(d11), NR^(c11)R^(d11),NR^(c11)NR^(c11)R^(d11), NR^(c11)C(O)R^(b11), NR^(c11)C(O)OR^(a11),NR^(c11)C(O)NR^(c11)R^(d11), C(═NR^(e11))R^(b11),C(═NR^(e11))NR^(c11)R^(d11), NR^(c11)C(═NR^(e11))NR^(c11)R^(d11),NR^(c11)C(═NR^(e11))R^(b11), NR^(c11)S(O)NR^(c11)R^(d11),NR^(c11)S(O)R^(b11), NR^(c11)S(O)₂R^(b11),NR^(c11)S(O)(═NR^(e11))R^(b11), NR^(c11)S(O)₂NR^(c11)R^(d11),S(O)R^(b11), S(O)NR^(c11)R^(d11), S(O)₂R^(b11), S(O)₂NR^(c11)R^(d11),OS(O)(═NR^(e11))R^(b11), OS(O)₂R^(b11), S(O)(═NR^(e11))R^(b11), SF₅,P(O)R^(f11)R^(g11), OP(O)(OR^(h11))(OR^(i11)), P(O)(OR^(h11))(OR^(i11))and BR^(j11)R^(k11), wherein said C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆alkynyl, C₁₋₆ haloalkyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10membered heterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(1B) substituents; each R^(1B) is independently selected from D, halo,CN, NO₂, C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₁₋₆ haloalkyl, C₃₋₇cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, 5-6 membered heteroaryl-C₁₋₄ alkyl,OR^(a12), SR^(a12), NHOR^(a12), C(O)R¹², C(O)NR^(c12)R¹²,C(O)NR^(c12)(OR^(a12)), C(O)OR^(a12), OC(O)R^(b12),OC(O)NR^(c12)R^(d12), NR^(c12)R^(d12), NR^(c12)NR^(c12)R^(d12),NR^(c12)C(O)R^(b12), NR^(c12)C(O)OR^(a12), NR¹²C(O)NR^(c12)R^(d12),C(═NR^(e12))R^(b12), C(═NR^(e12))NR^(c12)R^(d12),NR^(c12)C(═NR^(e12))NR^(c12)R^(d12), NR^(c12)C(═NR^(e12))R^(b12),NR^(c12)S(O)NR^(c12)R^(d12), NR^(c12)S(O)R^(b12), NR^(c12)S(O)₂R^(b12),NR^(c12)S(O)(═NR^(e12))R^(b12), NR^(c12)S(O)₂NR^(c12)R^(d12),S(O)R^(b12), S(O)NR^(c11)R^(d12), S(O)₂R^(b12), S(O)₂NR^(c12)R^(d12),OS(O)(═NR^(e12))R^(b12), OS(O)₂R^(b12), S(O)(═NR^(e12))R^(b12), SF₅,P(O)R^(f1)R^(g12), OP(O)(OR^(h12))(OR^(i12)), P(O)(OR^(h12))(OR^(i12)),and BR^(j12)R^(k12), wherein said C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆alkynyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl are each optionally substituted with 1,2, 3, or 4 independently selected R^(G) substituents; each R^(2A) isindependently selected from D, halo, CN, NO₂, C₁₋₆ alkyl, C₂₋₆ alkenyl,C₂₋₆ alkynyl, C₁₋₆ haloalkyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10membered heterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10 memberedheterocycloalkyl-C₁₋₄ alkyl, 5-10 membered heteroaryl-C₁₋₄ alkyl,OR^(a21), SR²¹, NHOR^(a21), C(O)R^(b21), C(O)NR^(c21)R^(d21),C(O)NR^(c21)(OR^(a21)), C(O)OR^(a21), OC(O)R^(b21),OC(O)NR^(c21)R^(d21), NR^(c21)R^(d21), NR^(c21)NR^(c21)R^(d21),NR^(c21)C(O)R^(b21), NR^(c21)C(O)OR^(a21), NR^(c21)C(O)NR^(c21)R^(d21),C(═NR^(e21))R^(b21), C(═NR^(e21))NR^(c21)R^(d21),NR^(c21)C(═NR^(e21))NR^(c21)R^(d21), NR^(c21)C(═NR^(e21))R^(b21),NR^(c21)S(O)NR^(c21)R^(d21), NR^(c21)S(O)R^(b21), NR^(c21)S(O)₂R^(b21),NR^(c21)S(O)(═NR^(e21))R^(b21), NR^(c21)S(O)₂NR^(d21), S(O)R^(b21),S(O)NR^(c21)R^(d21), S(O)₂R^(b21), S(O)₂NR^(c21)R^(d21),OS(O)(═NR^(e21))R^(b21), OS(O)₂R^(b21), S(O)(═NR^(e21))R^(b21), SF₅,P(O)R^(f21)R^(g21), OP(O)(OR^(h21))(OR^(i21)), P(O)(OR^(h21))(OR^(i21)),and BR^(j21)R^(k21), wherein said C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆alkynyl, C₁₋₆ haloalkyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10membered heterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(2B) substituents; each R^(2B) is independently selected from D, halo,CN, NO₂, C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₁₋₆ haloalkyl, C₃₋₇cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, 5-6 membered heteroaryl-C₁₋₄ alkyl,OR^(a22), SR^(a22), NHOR^(a22), C(O)R^(b22), C(O)NR^(c22)R^(d22),C(O)NR^(c22)(OR^(a22)), C(O)OR^(a22), OC(O)R^(b22),OC(O)NR^(c22)R^(d22), NR^(c22)R^(d22), NR^(c22)NR^(c22)R^(d22),NR^(c22)C(O)R^(b22)NR^(c22)C(O)OR^(a22), NR^(c22)C(O)NR^(c22)R^(d22),C(═NR^(e22))R^(b22), C(═NR^(e22))NR^(c22)R^(d22),NR^(c22)C(═NR^(e22))NR^(c22)R^(d22), NR^(c22)C(═NR^(e22))R^(b22),NR^(c22)S(O)NR^(c22)R^(d22), NR^(c22)S(O)R^(b22), NR^(c22)S(O)₂R^(b22),NR^(c22)S(O)(═NR^(e22))R^(b22), NR^(c22)S(O)₂NR^(c22)R^(d22),S(O)R^(b22), S(O)NR^(c22)R^(d22), S(O)₂R^(b22), S(O)₂NR^(c22)R^(d22),OS(O)(═NR^(e22))R^(b22), OS(O)₂R^(b22), S(O)(═NR^(e22))R^(b22), SF₅,P(O)R^(f22)R^(g22), OP(O)(OR^(h22))(OR^(i22)), P(O)(OR^(h22))(OR^(i22)),and BR^(j22)R^(k22), wherein said C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆alkynyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl are each optionally substituted with 1,2, 3, or 4 independently selected R^(G) substituents; each R^(8A) isselected from D, halo, CN, NO₂, C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl,C₁₋₆ haloalkyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10 memberedheterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl,6-10 membered aryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄alkyl, 5-10 membered heteroaryl-C₁₋₄ alkyl, OR^(a81), SRS, NHOR^(a81),C(O)R^(a81), C(O)NR^(c81)R^(d81), C(O)NR^(c81)(OR^(a81)), C(O)OR^(a81),OC(O)R^(a81), OC(O)NR^(c81)R^(d81), NR^(c81)R^(d81),NR^(c81)NR^(c81)R^(d81), NR^(c81)C(O)R^(b811), NR^(c81)C(O)OR^(a81),NR^(c81)C(O)NR^(c81)R^(d81), C(═NR^(e81))R^(b81),C(═NR^(e81))NR^(c81)R^(d81), NR^(c81)C(═NR^(e81))NR^(c81)R^(d81),NR^(c81)C(═NR^(e81))R^(b81), NR^(c81)S(O)NR^(c81)R^(d81),NR^(c81)S(O)R^(b81), NR^(c81)S(O)₂R^(b81),NR^(c81)S(O)(═NR^(e81))R^(b81), NR^(c81)S(O)₂NR^(c81)R^(d81),S(O)R^(b81), S(O)NR^(c81)R^(d81), S(O)₂R^(b81), S(O)₂NR^(c81)R^(d81),OS(O)(═NR^(e81))R^(b81), OS(O)₂R^(b81), S(O)(═NR^(e81))R^(b81), SF₅,P(O)R^(f81)R^(g81), OP(O)(OR^(h81))(OR^(i81)), P(O)(OR^(h81))(OR^(i81)),and BR^(j81)R^(k81); wherein said C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆alkynyl, C₁₋₆ haloalkyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10membered heterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted by 1, 2, 3, or 4 independently selectedR^(8B) substituents; each R^(8B) is selected from D, halo, CN, NO₂, C₁₋₆alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl,phenyl, 4-7 membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, 5-6 membered heteroaryl-C₁₋₄ alkyl,OR^(a82), SR^(a82), NHOR^(a82), C(O)R^(b82), C(O)NR^(c82)R^(d82),C(O)NR^(c82)(OR^(a2)), C(O)OR^(a82), OC(O)R^(b82), OC(O)NR^(c82)R^(d82),NR^(c82)R^(d82), NR^(c82)NR^(c82)R^(d82), NR^(c82)C(O)R^(b82),NR^(c82)C(O)OR^(a82), NR^(c82)C(O)NR^(c82)R^(d82), C(═NR^(e82))R^(b82),C(═NR^(e2))NR^(c82)R^(d82), NR^(c82)C(═NR^(e82))R^(c82)R^(d82),NR^(c82)C(═NR^(e82))R^(b82), NR^(c82)S(O)NR^(c82)R^(d82),NR^(c82)S(O)R^(b82), NR^(c82)S(O)₂R^(b82),NR^(c82)S(O)(═NR^(e82))R^(b82), NR^(c82)S(O)₂NR^(c82)R^(d82),S(O)R^(b82), S(O)NR^(c82)R^(d82), S(O)₂R^(b82), S(O)₂NR^(c82)R^(d82),OS(O)(═NR^(e82))R^(b82), OS(O)₂R^(b82), S(O)(═NR^(e82))R^(b82), SF₅,P(O)R^(f82)R^(g82), OP(O)(OR^(h82))(OR^(i82)), P(O)(OR^(h82))(OR^(i82)),and BR^(j82)R^(i82); wherein said C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆alkynyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl are each optionally substituted by 1, 2,3, or 4 independently selected R^(G) substituents; each R^(a1), R^(c1),and R^(d1) is independently selected from H, C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10membered heterocycloalkyl, 5-membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl,wherein said C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₁₋₆ haloalkyl,C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10 membered heterocycloalkyl,5-10 membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 memberedaryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-10membered heteroaryl-C₁₋₄ alkyl are each optionally substituted with 1,2, 3, or 4 independently selected R^(1A) substituents; or, any R^(c1)and R^(d1) attached to the same N atom, together with the N atom towhich they are attached, form a 4-10 membered heterocycloalkyl group,which is optionally substituted with 1, 2, 3, or 4 independentlyselected R^(1A) substituents; each R^(b1) is independently selected fromC₁₋₆ alkyl, C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀cycloalkyl, 6-10 membered aryl, 4-10 membered heterocycloalkyl, 5-10membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 memberedaryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-10membered heteroaryl-C₁₋₄ alkyl, which are each optionally substitutedwith 1, 2, 3, or 4 independently selected R^(1A) substituents; eachR^(e1) is independently selected from H, OH, CN, C₁₋₆ alkyl, C₁₋₆alkoxy, C₁₋₆ haloalkyl, C₁₋₆ haloalkoxy, C₂₋₆ alkenyl, C₂₋₆ alkynyl,C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10 membered heterocycloalkyl,5-10 membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 memberedaryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-10membered heteroaryl-C₁₋₄ alkyl; each R^(f1) and R^(g1) are independentlyselected from H, C₁₋₆ alkyl, C₁₋₆ alkoxy, C₁₋₆ haloalkyl, C₁₋₆haloalkoxy, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀ cycloalkyl, 6-10 memberedaryl, 4-10 membered heterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl;each R^(h1) and R^(i1) is independently selected from H, C₁₋₆ alkyl,C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀ cycloalkyl, 6-10membered aryl, 4-10 membered heterocycloalkyl, 5-10 membered heteroaryl,C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10membered heterocycloalkyl-C₁₋₄ alkyl, and 5-10 membered heteroaryl-C₁₋₄alkyl; each R^(j1) and R^(k1) is independently selected from OH, C₁₋₆alkoxy, and C₁₋₆ haloalkoxy; or any R^(j1) and R^(k1) attached to thesame B atom, together with the B atom to which they are attached, form a5- or 6-membered heterocycloalkyl group optionally substituted with 1,2, 3, or 4 substituents independently selected from C₁₋₆ alkyl and C₁₋₆haloalkyl; each R^(a11), R^(c11), and R^(d11) is independently selectedfrom H, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀cycloalkyl, 6-10 membered aryl, 4-10 membered heterocycloalkyl,5-membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 memberedaryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-10membered heteroaryl-C₁₋₄ alkyl, wherein said C₁₋₆ alkyl, C₂₋₆ alkenyl,C₂₋₆ alkynyl, C₁₋₆ haloalkyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10membered heterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(1B) substituents; or, any R^(c11) and R^(d11) attached to the same Natom, together with the N atom to which they are attached, form a 4-7membered heterocycloalkyl group, which is optionally substituted with 1,2, 3, or 4 independently selected R^(1B) substituents; each R^(b11), isindependently selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₂₋₆ alkenyl,C₂₋₆ alkynyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10 memberedheterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl,6-10 membered aryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl, which are eachoptionally substituted with 1, 2, 3, or 4 independently selected R^(1B)substituents; each R^(e11) is independently selected from H, OH, CN,C₁₋₆ alkyl, C₁₋₆ alkoxy, C₁₋₆ haloalkyl, C₁₋₆ haloalkoxy, C₂₋₆ alkenyl,C₂₋₆ alkynyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10 memberedheterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl,6-10 membered aryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl; each R^(f11) and R^(g11)are independently selected from H, C₁₋₆ alkyl, C₁₋₆ alkoxy, C₁₋₆haloalkyl, C₁₋₆ haloalkoxy, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀cycloalkyl, 6-10 membered aryl, 4-10 membered heterocycloalkyl, 5-10membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 memberedaryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-10membered heteroaryl-C₁₋₄ alkyl; each R^(h11) and R^(i11) isindependently selected from H, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₂₋₆ alkenyl,C₂₋₆ alkynyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10 memberedheterocycloalkyl, 5-membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl,6-10 membered aryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl; each R^(j11) and R^(k11)is independently selected from OH, C₁₋₆ alkoxy, and C₁₋₆ haloalkoxy; orany R^(j11) and R^(k11) attached to the same B atom, together with the Batom to which they are attached, form a 5- or 6-memberedheterocycloalkyl group optionally substituted with 1, 2, 3, or 4substituents independently selected from C₁₋₆ alkyl and C₁₋₆ haloalkyl;each R^(a12), R^(c12), and R^(d12) is independently selected from H,C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₇ cycloalkyl,phenyl, 4-7 membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl,wherein said C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(G) substituents; or, any R^(c12) and R^(d12) attached to the same Natom, together with the N atom to which they are attached, form a 4-7membered heterocycloalkyl group, which is optionally substituted with 1,2, 3, or 4 independently selected R^(G) substituents; each R^(b12) isindependently selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₂₋₆ alkenyl,C₂₋₆ alkynyl, C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl,5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl,4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6 memberedheteroaryl-C₁₋₄ alkyl, which are each optionally substituted with 1, 2,3, or 4 independently selected R^(G) substituents; each R^(e12) isindependently selected from H, OH, CN, C₁₋₆ alkyl, C₁₋₆ alkoxy, C₁₋₆haloalkyl, C₁₋₆ haloalkoxy, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₇ cycloalkyl,phenyl, 4-7 membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl;each R^(f12) and R^(g12) are independently selected from H, C₁₋₆ alkyl,C₁₋₆ alkoxy, C₁₋₆ haloalkyl, C₁₋₆ haloalkoxy, C₂₋₆ alkenyl, C₂₋₆alkynyl, C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄alkyl; each R^(h12) and R^(i12) is independently selected from H, C₁₋₆alkyl, C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₇ cycloalkyl,phenyl, 4-7 membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl;each R^(j12) and R^(k12) is independently selected from OH, C₁₋₆ alkoxy,and C₁₋₆ haloalkoxy; or any R^(j12) and R^(k12) attached to the same Batom, together with the B atom to which they are attached, form a 5- or6-membered heterocycloalkyl group optionally substituted with 1, 2, 3,or 4 substituents independently selected from C₁₋₆ alkyl and C₁₋₆haloalkyl; each R^(a2), R^(c2), and R^(d2) is independently selectedfrom H, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀cycloalkyl, 6-10 membered aryl, 4-10 membered heterocycloalkyl,5-membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 memberedaryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-10membered heteroaryl-C₁₋₄ alkyl, wherein said C₁₋₆ alkyl, C₂₋₆ alkenyl,C₂₋₆ alkynyl, C₁₋₆ haloalkyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10membered heterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(2A) substituents; or, any R^(c2) and R^(d2) attached to the same Natom, together with the N atom to which they are attached, form a 4-10membered heterocycloalkyl group, which is optionally substituted with 1,2, 3, or 4 independently selected R^(2A) substituents; each R^(b2) isindependently selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₂₋₆ alkenyl,C₂₋₆ alkynyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10 memberedheterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl,6-10 membered aryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl, which are eachoptionally substituted with 1, 2, 3, or 4 independently selected R^(2A)substituents; each R^(e2) is independently selected from H, OH, CN, C₁₋₆alkyl, C₁₋₆ alkoxy, C₁₋₆ haloalkyl, C₁₋₆ haloalkoxy, C₂₋₆ alkenyl, C₂₋₆alkynyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10 memberedheterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl,6-10 membered aryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl; each R^(f2) and R^(g2)are independently selected from H, C₁₋₆ alkyl, C₁₋₆ alkoxy, C₁₋₆haloalkyl, C₁₋₆ haloalkoxy, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀cycloalkyl, 6-10 membered aryl, 4-10 membered heterocycloalkyl, 5-10membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 memberedaryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-10membered heteroaryl-C₁₋₄ alkyl; each R^(h2) and R^(i2) is independentlyselected from H, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl,C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10 membered heterocycloalkyl,5-10 membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 memberedaryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-10membered heteroaryl-C₁₋₄ alkyl; each R^(j2) and R^(k2) is independentlyselected from OH, C₁₋₆ alkoxy, and C₁₋₆ haloalkoxy; or any R^(j2) andR^(k2) attached to the same B atom, together with the B atom to whichthey are attached, form a 5- or 6-membered heterocycloalkyl groupoptionally substituted with 1, 2, 3, or 4 substituents independentlyselected from C₁₋₆ alkyl and C₁₋₆ haloalkyl; each R^(a21), R^(c21), andR^(d21) is independently selected from H, C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10membered heterocycloalkyl, 5-membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl,wherein said C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₁₋₆ haloalkyl,C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10 membered heterocycloalkyl,5-10 membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 memberedaryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-10membered heteroaryl-C₁₋₄ alkyl are each optionally substituted with 1,2, 3, or 4 independently selected R^(2B) substituents; or, any R^(c21)and R^(d21) attached to the same N atom, together with the N atom towhich they are attached, form a 4-7 membered heterocycloalkyl group,which is optionally substituted with 1, 2, 3, or 4 independentlyselected R^(2B) substituents; each R^(b21) is independently selectedfrom C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀cycloalkyl, 6-10 membered aryl, 4-10 membered heterocycloalkyl, 5-10membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 memberedaryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-10membered heteroaryl-C₁₋₄ alkyl, which are each optionally substitutedwith 1, 2, 3, or 4 independently selected R^(2B) substituents; eachR^(e21) is independently selected from H, OH, CN, C₁₋₆ alkyl, C₁₋₆alkoxy, C₁₋₆ haloalkyl, C₁₋₆ haloalkoxy, C₂₋₆ alkenyl, C₂₋₆ alkynyl,C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10 membered heterocycloalkyl,5-10 membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 memberedaryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-10membered heteroaryl-C₁₋₄ alkyl; each R^(f21) and R^(g21) areindependently selected from H, C₁₋₆ alkyl, C₁₋₆ alkoxy, C₁₋₆ haloalkyl,C₁₋₆ haloalkoxy, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀ cycloalkyl, 6-10membered aryl, 4-10 membered heterocycloalkyl, 5-10 membered heteroaryl,C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10membered heterocycloalkyl-C₁₋₄ alkyl, and 5-10 membered heteroaryl-C₁₋₄alkyl; each R^(h21) and R^(i21) is independently selected from H, C₁₋₆alkyl, C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀ cycloalkyl,6-10 membered aryl, 4-10 membered heterocycloalkyl, 5-memberedheteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl,4-10 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-10 memberedheteroaryl-C₁₋₄ alkyl; each R^(j21) and R^(k21) is independentlyselected from OH, C₁₋₆ alkoxy, and C₁₋₆ haloalkoxy; or any R^(j21) andR^(k21) attached to the same B atom, together with the B atom to whichthey are attached, form a 5- or 6-membered heterocycloalkyl groupoptionally substituted with 1, 2, 3, or 4 substituents independentlyselected from C₁₋₆ alkyl and C₁₋₆ haloalkyl; each R^(a22), R^(c22), andR^(d22) is independently selected from H, C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl, wherein said C₁₋₆ alkyl, C₂₋₆ alkenyl,C₂₋₆ alkynyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl are each optionally substituted with 1,2, 3, or 4 independently selected R^(G) substituents; or, any R^(c22)and R^(d22) attached to the same N atom, together with the N atom towhich they are attached, form a 4-7 membered heterocycloalkyl group,which is optionally substituted with 1, 2, 3, or 4 independentlyselected R^(G) substituents; each R^(b22) is independently selected fromC₁₋₆ alkyl, C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₇ cycloalkyl,phenyl, 4-7 membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl,which are each optionally substituted with 1, 2, 3, or 4 independentlyselected R^(G) substituents; each R^(e22) is independently selected fromH, OH, CN, C₁₋₆ alkyl, C₁₋₆ alkoxy, C₁₋₆ haloalkyl, C₁₋₆ haloalkoxy,C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl; each R^(f22) and R^(g22) areindependently selected from H, C₁₋₆ alkyl, C₁₋₆ alkoxy, C₁₋₆ haloalkyl,C₁₋₆ haloalkoxy, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₇ cycloalkyl, phenyl,4-7 membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl;each R^(h22) and R^(i22) is independently selected from H, C₁₋₆ alkyl,C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄alkyl, phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and5-6 membered heteroaryl-C₁₋₄ alkyl; each R^(j22) and R^(k22) isindependently selected from OH, C₁₋₆ alkoxy, and C₁₋₆ haloalkoxy; or anyR^(j22) and R^(k22) attached to the same B atom, together with the Batom to which they are attached, form a 5- or 6-memberedheterocycloalkyl group optionally substituted with 1, 2, 3, or 4substituents independently selected from C₁₋₆ alkyl and C₁₋₆ haloalkyl;each R^(a8), R^(c8), and R^(d8) is independently selected from H, C₁₋₆alkyl, C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀ cycloalkyl,6-10 membered aryl, 4-10 membered heterocycloalkyl, 5-memberedheteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl,4-10 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-10 memberedheteroaryl-C₁₋₄ alkyl, wherein said C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆alkynyl, C₁₋₆ haloalkyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10membered heterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(8A) substituents; or, any R^(c8) and R^(d8) attached to the same Natom, together with the N atom to which they are attached, form a 4-10membered heterocycloalkyl group, which is optionally substituted with 1,2, 3, or 4 independently selected R^(8A) substituents; each R^(b8) isindependently selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₂₋₆ alkenyl,C₂₋₆ alkynyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10 memberedheterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl,6-10 membered aryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl, which are eachoptionally substituted with 1, 2, 3, or 4 independently selected R^(8A)substituents; each R^(e8) is independently selected from H, OH, CN, C₁₋₆alkyl, C₁₋₆ alkoxy, C₁₋₆ haloalkyl, C₁₋₆ haloalkoxy, C₂₋₆ alkenyl, C₂₋₆alkynyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10 memberedheterocycloalkyl, 5-10 membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl,6-10 membered aryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl; each R^(f8) and R^(g8)are independently selected from H, C₁₋₆ alkyl, C₁₋₆ alkoxy, C₁₋₆haloalkyl, C₁₋₆ haloalkoxy, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀cycloalkyl, 6-10 membered aryl, 4-10 membered heterocycloalkyl, 5-10membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 memberedaryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-10membered heteroaryl-C₁₋₄ alkyl; each R^(h8) and R^(i8) is independentlyselected from H, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl,C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10 membered heterocycloalkyl,5-10 membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 memberedaryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-10membered heteroaryl-C₁₋₄ alkyl; each R^(j8) and R^(k8) is independentlyselected from OH, C₁₋₆ alkoxy, and C₁₋₆ haloalkoxy; or any R^(j8) andR^(k8) attached to the same B atom, together with the B atom to whichthey are attached, form a 5- or 6-membered heterocycloalkyl groupoptionally substituted with 1, 2, 3, or 4 substituents independentlyselected from C₁₋₆ alkyl and C₁₋₆ haloalkyl; each R^(a81), R^(c81), andR^(d81) is independently selected from H, C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10membered heterocycloalkyl, 5-membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-10 membered heteroaryl-C₁₋₄ alkyl,wherein said C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₁₋₆ haloalkyl,C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10 membered heterocycloalkyl,5-10 membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 memberedaryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-10membered heteroaryl-C₁₋₄ alkyl are each optionally substituted with 1,2, 3, or 4 independently selected R^(8B) substituents; or, any R^(c81)and R^(d81) attached to the same N atom, together with the N atom towhich they are attached, form a 4-7 membered heterocycloalkyl group,which is optionally substituted with 1, 2, 3, or 4 independentlyselected R^(8B) substituents; each R^(b81) is independently selectedfrom C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀cycloalkyl, 6-10 membered aryl, 4-10 membered heterocycloalkyl, 5-10membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 memberedaryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-10membered heteroaryl-C₁₋₄ alkyl, which are each optionally substitutedwith 1, 2, 3, or 4 independently selected R^(8B) substituents; eachR^(c81) is independently selected from H, OH, CN, C₁₋₆ alkyl, C₁₋₆alkoxy, C₁₋₆ haloalkyl, C₁₋₆ haloalkoxy, C₂₋₆ alkenyl, C₂₋₆ alkynyl,C₃₋₁₀ cycloalkyl, 6-10 membered aryl, 4-10 membered heterocycloalkyl,5-10 membered heteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 memberedaryl-C₁₋₄ alkyl, 4-10 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-10membered heteroaryl-C₁₋₄ alkyl; each R^(f81) and R^(g81) areindependently selected from H, C₁₋₆ alkyl, C₁₋₆ alkoxy, C₁₋₆ haloalkyl,C₁₋₆ haloalkoxy, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀ cycloalkyl, 6-10membered aryl, 4-10 membered heterocycloalkyl, 5-10 membered heteroaryl,C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl, 4-10membered heterocycloalkyl-C₁₋₄ alkyl, and 5-10 membered heteroaryl-C₁₋₄alkyl; each R^(h81) and R^(i81) is independently selected from H, C₁₋₆alkyl, C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀ cycloalkyl,6-10 membered aryl, 4-10 membered heterocycloalkyl, 5-memberedheteroaryl, C₃₋₁₀ cycloalkyl-C₁₋₄ alkyl, 6-10 membered aryl-C₁₋₄ alkyl,4-10 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-10 memberedheteroaryl-C₁₋₄ alkyl; each R^(j81) and R^(k81) is independentlyselected from OH, C₁₋₆ alkoxy, and C₁₋₆ haloalkoxy; or any R^(j81) andR^(k81) attached to the same B atom, together with the B atom to whichthey are attached, form a 5- or 6-membered heterocycloalkyl groupoptionally substituted with 1, 2, 3, or 4 substituents independentlyselected from C₁₋₆ alkyl and C₁₋₆ haloalkyl; each R^(a82), R^(c82), andR^(d82) is independently selected from H, C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl, wherein said C₁₋₆ alkyl, C₂₋₆ alkenyl,C₂₋₆ alkynyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl are each optionally substituted with 1,2, 3, or 4 independently selected R^(G) substituents; or, any R^(c82)and R^(d82) attached to the same N atom, together with the N atom towhich they are attached, form a 4-7 membered heterocycloalkyl group,which is optionally substituted with 1, 2, 3, or 4 independentlyselected R^(G) substituents; each R^(b82) is independently selected fromC₁₋₆ alkyl, C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₇ cycloalkyl,phenyl, 4-7 membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl,which are each optionally substituted with 1, 2, 3, or 4 independentlyselected R^(G) substituents; each R^(e82) is independently selected fromH, OH, CN, C₁₋₆ alkyl, C₁₋₆ alkoxy, C₁₋₆ haloalkyl, C₁₋₆ haloalkoxy,C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl; each R^(f82) and R^(g82) areindependently selected from H, C₁₋₆ alkyl, C₁₋₆ alkoxy, C₁₋₆ haloalkyl,C₁₋₆ haloalkoxy, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₇ cycloalkyl, phenyl,4-7 membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl;each R^(h82) and R^(i82) is independently selected from H, C₁₋₆ alkyl,C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄alkyl, phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and5-6 membered heteroaryl-C₁₋₄ alkyl; each R^(j82) and R^(k82) isindependently selected from OH, C₁₋₆ alkoxy, and C₁₋₆ haloalkoxy; or anyR^(j82) and R^(k82) attached to the same B atom, together with the Batom to which they are attached, form a 5- or 6-memberedheterocycloalkyl group optionally substituted with 1, 2, 3, or 4substituents independently selected from C₁₋₆ alkyl and C₁₋₆ haloalkyl;and each R^(G) is independently selected from OH, NO₂, CN, halo, C₁₋₃alkyl, C₂₋₃ alkenyl, C₂₋₃ alkynyl, C₁₋₃ haloalkyl, cyano-C₁₋₃ alkyl,HO—C₁₋₃ alkyl, C₁₋₃ alkoxy-C₁₋₃ alkyl, C₃₋₇ cycloalkyl, C₁₋₃ alkoxy,C₁₋₃ haloalkoxy, amino, C₁₋₃ alkylamino, di(C₁₋₃ alkyl)amino, thio, C₁₋₃alkylthio, C₁₋₃ alkylsulfinyl, C₁₋₃ alkylsulfonyl, carbamyl, C₁₋₃alkylcarbamoyl, di(C₁₋₃ alkyl)carbamyl, carboxy, C₁₋₃ alkylcarbonyl,C₁₋₃ alkoxycarbonyl, C₁₋₃ alkylcarbonyloxy, C₁₋₃ alkylcarbonylamino,C₁₋₃ alkoxycarbonylamino, C₁₋₃ alkylaminocarbonyloxy, di(C₁₋₃alkyl)aminocarbonyloxy, C₁₋₃ alkylsulfonylamino, aminosulfonyl, C₁₋₃alkylaminosulfonyl, di(C₁₋₃ alkyl)aminosulfonyl, aminosulfonylamino,C₁₋₃ alkylaminosulfonylamino, di(C₁₋₃ alkyl)aminosulfonylamino,aminocarbonylamino, C₁₋₃ alkylaminocarbonylamino, and di(C₁₋₃alkyl)aminocarbonylamino.
 2. The compound of claim 1, or apharmaceutically acceptable salt thereof, wherein Ring


3. The compound of any one of claims 1-2, or a pharmaceuticallyacceptable salt thereof, wherein R¹ is selected from H, halo, CN, C₁₋₆alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl; wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted by 1, 2, 3, or 4 independently selectedR^(1A) substituents.
 4. The compound of any one of claims 1-2, or apharmaceutically acceptable salt thereof, wherein R¹ is selected from H,halo, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, and 5-6 membered heteroaryl; wherein said C₁₋₆ alkyl,C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl,and 5-6 membered heteroaryl are each optionally substituted by 1, 2, or3 independently selected R^(1A) substituents.
 5. The compound of any oneof claims 1-2, or a pharmaceutically acceptable salt thereof, wherein R¹is selected from H, halo, C₁₋₆ alkyl, phenyl, and 5-6 memberedheteroaryl, wherein said C₁₋₆ alkyl, phenyl and 5-6 membered heteroarylare each optionally substituted by 1 or 2 independently selected R^(1A)substituents.
 6. The compound of any one of claims 1-5, or apharmaceutically acceptable salt thereof, wherein R^(2x) is selectedfrom H, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, and 5-6 membered heteroaryl, wherein saidC₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, and 5-6 membered heteroaryl are each optionallysubstituted by 1, 2, or 3 independently selected R^(2A) substituents. 7.The compound of any one of claims 1-5, or a pharmaceutically acceptablesalt thereof, wherein R^(2x) is selected from H, C₁₋₆ alkyl, C₃₋₇cycloalkyl, and phenyl-C₁₋₃ alkyl.
 8. The compound of claim 1, or apharmaceutically acceptable salt thereof, wherein Ring


9. The compound of claim 1 or 8, or a pharmaceutically acceptable saltthereof, wherein RX is selected from H, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl;wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄alkyl, phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and5-6 membered heteroaryl-C₁₋₄ alkyl are each optionally substituted by 1,2, or 3 independently selected R^(1A) substituents.
 10. The compound ofclaim 1 or 8, or a pharmaceutically acceptable salt thereof, wherein RXis selected from H, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl,4-7 membered heterocycloalkyl, and 5-6 membered heteroaryl; wherein saidC₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, and 5-6 membered heteroaryl are each optionallysubstituted by 1, 2, or 3 independently selected R^(1A) substituents.11. The compound of claim 1 or 8, or a pharmaceutically acceptable saltthereof, wherein RX is selected from H, C₁₋₆ alkyl, C₃₋₇ cycloalkyl,phenyl, and 4-7 membered heterocycloalkyl, wherein said C₁₋₆ alkyl, C₃₋₇cycloalkyl, phenyl, and 4-7 membered heterocycloalkyl are eachoptionally substituted by 1 or 2 independently selected R^(1A)substituents.
 12. The compound of any one of claims 1 and 8-11, or apharmaceutically acceptable salt thereof, wherein R² is selected from H,halo, CN, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄alkyl, phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and5-6 membered heteroaryl-C₁₋₄ alkyl; wherein said C₁₋₆ alkyl, C₁₋₆haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄alkyl are each optionally substituted by 1, 2, 3, or 4 independentlyselected R^(2A) substituents.
 13. The compound of any one of claims 1and 8-11, or a pharmaceutically acceptable salt thereof, wherein R² isselected from H, halo, CN, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl,phenyl, 4-7 membered heterocycloalkyl, and 5-6 membered heteroaryl;wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, and 5-6 membered heteroaryl are eachoptionally substituted by 1, 2, or 3 independently selected R^(2A)substituents.
 14. The compound of any one of claims 1 and 8-11, or apharmaceutically acceptable salt thereof, wherein R² is selected from H,C₁₋₆ alkyl, and C₃₋₇ cycloalkyl.
 15. The compound of any one of claims1-14 or a pharmaceutically acceptable salt thereof, wherein: eachR^(a1), R^(c1), and R^(d1) is independently selected from H, C₁₋₆ alkyl,and C₁₋₆ haloalkyl, wherein said C₁₋₆ alkyl and C₁₋₆ haloalkyl are eachoptionally substituted with 1, 2, 3, or 4 independently selected R^(1A)substituents; each R^(b1) is independently selected from C₁₋₆ alkyl andC₁₋₆ haloalkyl, which are each optionally substituted with 1, 2, 3, or 4independently selected R^(1A) substituents; each R^(1A) is independentlyselected from halo, CN, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl,OR^(a11), and NR^(c11)R^(d11); and each R^(a11), R^(c11), and R^(d11) isindependently selected from H, C₁₋₆ alkyl and C₁₋₆ haloalkyl.
 16. Thecompound of any one of claims 1-15, or a pharmaceutically acceptablesalt thereof, wherein: each R^(a2), R^(c2), and R^(d2) is independentlyselected from H, C₁₋₆ alkyl, and C₁₋₆ haloalkyl, wherein said C₁₋₆ alkyland C₁₋₆ haloalkyl are each optionally substituted with 1, 2, 3, or 4independently selected R^(2A) substituents; each R^(b2) is independentlyselected from C₁₋₆ alkyl and C₁₋₆ haloalkyl, which are each optionallysubstituted with 1, 2, 3, or 4 independently selected R^(2A)substituents; each R^(2A) is independently selected from halo, CN, NO₂,C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl,OR^(a21), and NR^(c21)R^(d21); and each R^(a21), R^(c21), and R^(d21) isindependently selected from H, C₁₋₆ alkyl and C₁₋₆ haloalkyl.
 17. Thecompound of any one of claims 1-16, or a pharmaceutically acceptablesalt thereof, wherein R³ is H.
 18. The compound of any one of claims1-17, or a pharmaceutically acceptable salt thereof, wherein Y is N. 19.The compound of any one of claims 1-18, or a pharmaceutically acceptablesalt thereof, wherein Z¹ is CR⁴R⁵; and Z² is CR^(4z)R^(5z) or O.
 20. Thecompound of any one of claims 1-19, or a pharmaceutically acceptablesalt thereof, wherein R⁴, R^(4z), R⁵ and R^(5z) are each independentlyselected from H and C₁₋₃ alkyl.
 21. The compound of any one of claims1-20, or a pharmaceutically acceptable salt thereof, wherein Ring moietyA is an azetidine ring, a pyrrolidine ring, a piperidine ring, or anazepane ring.
 22. The compound of any one of claims 1-20, or apharmaceutically acceptable salt thereof, wherein Ring moiety A ispiperidin-4-yl.
 23. The compound of any one of claims 1-22, or apharmaceutically acceptable salt thereof, wherein n is 1 or
 2. 24. Thecompound of any one of claims 1-23, or a pharmaceutically acceptablesalt thereof, wherein at least one R⁸ is S(O)₂R^(b8).
 25. The compoundof any one of claims 1-23, or a pharmaceutically acceptable saltthereof, wherein one R⁸ is S(O)₂R^(b8), wherein R^(b8) is independentlyselected from C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄alkyl, phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and5-6 membered heteroaryl-C₁₋₄ alkyl, which are each optionallysubstituted with 1, 2, 3, or 4 independently selected R^(8A)substituents.
 26. The compound of any one of claims 1-23, or apharmaceutically acceptable salt thereof, wherein one R⁸ is S(O)₂R^(b8),wherein R^(b8) is independently selected from selected from C₁₋₆ alkyl,C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl,and 5-6 membered heteroaryl, each of which is optionally substituted by1, 2, 3, or 4 independently selected R^(8A) substituents.
 27. Thecompound of any one of claims 1-23, or a pharmaceutically acceptablesalt thereof, wherein one R⁸ is halo or C₁₋₆ alkyl and one R⁸ isS(O)₂R^(b8), wherein R^(b8) is selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, and 5-6 memberedheteroaryl, each of which is optionally substituted by 1, 2, 3, or 4independently selected R^(8A) substituents.
 28. The compound of any oneof claims 1-26, or a pharmaceutically acceptable salt thereof, wherein:each R^(a8), R^(c8), and R^(d8) is independently selected from H, C₁₋₆alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl, wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(8A) substituents; each R^(b8) is independently selected from C₁₋₆alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl, which are each optionally substitutedwith 1, 2, 3, or 4 independently selected R^(8A) substituents; eachR^(8A) is independently selected from halo, CN, NO₂, C₁₋₆ alkyl, C₁₋₆haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7membered heterocycloalkyl-C₁₋₄ alkyl, 5-6 membered heteroaryl-C₁₋₄alkyl, OR^(a81), SR^(a81), C(O)R^(b81), C(O)NR^(c81)R^(d81),C(O)OR^(a81), OC(O)R^(b81), OC(O)NR^(c81)R^(d81), NR^(c81)R^(d81),NR^(c11)C(O)R^(b81), NR^(c11)C(O)OR^(a81), NR^(c81)C(O)NR^(c81)R^(d81),NR^(c81)S(O)₂R^(b81), NR^(c81)S(O)₂NR^(c81)R^(d81), S(O)₂R^(b81), andS(O)₂NR^(c81)R^(d81), wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(8B) substituents; each R^(a81), R^(c81), and R^(d81) is independentlyselected from H, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl,4-7 membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl,wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄alkyl, phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and5-6 membered heteroaryl-C₁₋₄ alkyl are each optionally substituted with1, 2, 3, or 4 independently selected R^(8B) substituents; each R^(b81)is independently selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl,which are each optionally substituted with 1, 2, 3, or 4 independentlyselected R^(8B) substituents; each R^(8B) is independently selected fromhalo, CN, NO₂, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₄ cycloalkyl, 4-5 memberedheterocycloalkyl, OR^(a82), and NR^(c82)R^(d82); and each R^(a82),R^(e82), and R^(d82) is independently selected from H, C₁₋₆ alkyl, andC₁₋₆ haloalkyl.
 29. The compound of any one of claims 1-27, or apharmaceutically acceptable salt thereof, wherein: each R^(8A) isindependently selected from halo, CN, C₁₋₆ alkyl, C₁₋₆ haloalkyl, and4-7 membered heterocycloalkyl; wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl,and 4-7 membered heterocycloalkyl are each optionally substituted by 1or 2 independently selected R^(8B) substituents; each R^(8B) is selectedfrom C₁₋₆ alkyl and OR^(a82); and each R^(a82) is independently selectedfrom Hand C₁₋₆ alkyl.
 30. The compound of claim 1, or a pharmaceuticallyacceptable salt thereof, wherein: n is 1, 2, 3, or 4; Z¹ is CR⁴R⁵; andZ² is NR^(6z), O, or S; or Z¹ is CR⁴R⁵, NR⁶, O, or S; and Z² isCR^(4z)R^(5z); Y is CR⁷ or N; X¹ is NR^(1x) or CR¹; X² is NR^(2x) orCR²; X³ is N or C; wherein Ring

is an aromatic ring selected from:

Ring moiety A is monocyclic or bicyclic 4-10 membered heterocycloalkylhaving 1, 2, 3, or 4 ring-forming heteroatoms independently selectedfrom N, O, and S, wherein 1, 2, 3, or 4 ring-forming carbon orheteroatoms can be optionally substituted by one or more oxo or sulfido,wherein said heterocycloalkyl does not comprise an aromatic ring; R¹ isselected from H, halo, CN, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl,phenyl, 4-7 membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, 5-6 membered heteroaryl-C₁₋₄ alkyl,OR^(a1), SR^(a1), C(O)R^(b1), C(O)NR^(c1)R^(d1), C(O)OR^(a1),OC(O)R^(b1), OC(O)NR^(c1)R^(d1), NR^(c1)R^(d1), NR C(O)R^(b1),NR^(c1)C(O)OR^(a1), NR^(c1)C(O)NR^(c1)R^(d1), NR^(c11)S(O)₂R^(b1),NR^(c1)S(O)₂NR^(c1)R^(d1), S(O)₂R^(b1), and S(O)₂NR^(c1)R^(d1); whereinsaid C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl are each optionally substituted by 1, 2,3, or 4 independently selected R^(1A) substituents; R^(1x) is selectedfrom H, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄alkyl, phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and5-6 membered heteroaryl-C₁₋₄ alkyl; wherein said C₁₋₆ alkyl, C₁₋₆haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄alkyl are each optionally substituted by 1, 2, or 3 independentlyselected R^(1A) substituents; R² is selected from H, halo, CN, C₁₋₆alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, 5-6membered heteroaryl-C₁₋₄ alkyl, OR^(a2) SR^(a2), C(O)R^(b2),C(O)NR^(c2)R^(d2), C(O)OR^(a2), OC(O)R^(b2), OC(O)NR^(c2)R^(d2),NR^(c2)R^(d2), NR^(c2)C(O)R^(b2),NR^(c2)C(O)OR^(a2)NR^(c2)C(O)NR^(c2)R^(d2), NR^(c2)S(O)₂R^(b2),NR^(c2)S(O)₂NR^(c2)R^(d2), S(O)₂R^(b2), and S(O)₂NR^(c2)R^(d2); whereinsaid C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl are each optionally substituted by 1, 2,3, or 4 independently selected R^(2A) substituents; R^(2x) is selectedfrom H, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄alkyl, phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and5-6 membered heteroaryl-C₁₋₄ alkyl, wherein said C₁₋₆ alkyl, C₁₋₆haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄alkyl are each optionally substituted by 1, 2, or 3 independentlyselected R^(2A) substituents; R³ is selected from H, halo, CN, C₁₋₃alkyl, and C₁₋₃ haloalkyl; R⁴, R^(4z), R⁵ and R^(5z) are independentlyselected from H, halo, CN, C₁₋₃ alkyl, and C₁₋₃ haloalkyl; or R⁴ and R⁵,together with the carbon atom to which they are attached, form acyclopropyl or cyclobutyl ring; or R^(4z) and R^(5z), together with thecarbon atom to which they are attached, form a 3 cyclopropyl orcyclobutyl ring; R⁷ is selected from H, halo, CN, C₁₋₃ alkyl, and C₁₋₃haloalkyl; R⁸ is selected from H, halo, CN, C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, 5-6 membered heteroaryl-C₁₋₄ alkyl,OR^(a8), SR^(a8), C(O)R^(b8), C(O)NR^(c8)R^(d8), C(O)OR^(a8),OC(O)R^(b8), OC(O)NR^(c8)R^(d8), NR^(c8)R^(d8), NR^(c8)C(O)R^(b8),NR^(c8)C(O)OR^(a8), NR^(c8)C(O)NR^(c8)R^(d8), NR^(c8)S(O)₂R^(b8),NR^(c8)S(O)₂NR^(c8)R^(d8), S(O)₂R^(b8), and S(O)₂NR^(c8)R^(d8); whereinsaid C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl are each optionally substituted by 1, 2,3, or 4 independently selected R^(8A) substituents; each R^(a1), R^(c1),and R^(d1) is independently selected from H, C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl,wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄alkyl, phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and5-6 membered heteroaryl-C₁₋₄ alkyl are each optionally substituted with1, 2, 3, or 4 independently selected R^(1A) substituents; each R^(b1) isindependently selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl,phenyl, 4-7 membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl,which are each optionally substituted with 1, 2, 3, or 4 independentlyselected R^(1A) substituents; each R^(1A) is independently selected fromhalo, CN, NO₂, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄alkyl, phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, 5-6membered heteroaryl-C₁₋₄ alkyl, OR^(a11), SR^(a11), C(O)R^(b11),C(O)NR^(c11)R^(d11), C(O)OR^(a11), OC(O)R^(b11), OC(O)NR^(c11)R^(d11),NR^(c11)R^(d11), NR^(c11)C(O)R^(b11), NR^(c11)C(O)OR^(a11),NR^(c11)C(O)NR^(c11)R^(d11), NR^(c11)S(O)₂R^(b11),NR^(c11)S(O)₂NR^(c11)R^(d11), S(O)₂R^(b11), and S(O)₂NR^(c11)R^(d11),wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄alkyl, phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and5-6 membered heteroaryl-C₁₋₄ alkyl are each optionally substituted with1, 2, 3, or 4 independently selected R^(1B) substituents; each R^(a11),R^(c11), and R^(d11) is independently selected from H, C₁₋₆ alkyl, C₁₋₆haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄alkyl, wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl,4-7 membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(1B) substituents; each R^(b11) is independently selected from C₁₋₆alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl, which are each optionally substitutedwith 1, 2, 3, or 4 independently selected R^(1B) substituents; eachR^(1B) is independently selected from halo, CN, NO₂, C₁₋₆ alkyl, C₁₋₆haloalkyl, C₃₋₄ cycloalkyl, 4-5 membered heterocycloalkyl, OR^(a12), andNR^(c12)R^(d12); each R^(a12), R^(c12), and R^(d12) is independentlyselected from H, C₁₋₆ alkyl, and C₁₋₆ haloalkyl; each R^(a2), R^(c2),and R^(d2) is independently selected from H, C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl,wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄alkyl, phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and5-6 membered heteroaryl-C₁₋₄ alkyl are each optionally substituted with1, 2, 3, or 4 independently selected R^(2A) substituents; each R^(b2) isindependently selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl,phenyl, 4-7 membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl,which are each optionally substituted with 1, 2, 3, or 4 independentlyselected R^(2A) substituents; each R^(2A) is independently selected fromhalo, CN, NO₂, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄alkyl, phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, 5-6membered heteroaryl-C₁₋₄ alkyl, OR^(a21), SR^(a21), C(O)R^(b21),C(O)NR^(c21)R^(d21), C(O)OR^(a21), OC(O)R^(b21), OC(O)NR^(c21)R^(d21),NR^(c21)R^(d21), NR^(c21)C(O)R^(b21), NR^(c21)C(O)OR^(a21),NR^(c21)C(O)NR^(c21)R^(d21), NR^(c21)S(O)₂R^(b21),NR^(c21)S(O)₂NR^(c21)R^(d21), S(O)₂R^(b21), and S(O)₂NR^(c21)R^(d21),wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄alkyl, phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and5-6 membered heteroaryl-C₁₋₄ alkyl are each optionally substituted with1, 2, 3, or 4 independently selected R^(2B) substituents; each R^(a21),R^(c21), and R^(d21) is independently selected from H, C₁₋₆ alkyl, C₁₋₆haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄alkyl, wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl,4-7 membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selected R²substituents; each R^(b21) is independently selected from C₁₋₆ alkyl,C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl,5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl,4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6 memberedheteroaryl-C₁₋₄ alkyl, which are each optionally substituted with 1, 2,3, or 4 independently selected R² substituents; each R^(2B) isindependently selected from halo, CN, NO₂, C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₄ cycloalkyl, 4-5 membered heterocycloalkyl, OR^(a22), andNR^(c22)R^(d22); each R^(a22), R^(c22), and R^(d22) is independentlyselected from H, C₁₋₆ alkyl, and C₁₋₆ haloalkyl; each R^(a8), R^(c8),and R^(d8) is independently selected from H, C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl,wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄alkyl, phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and5-6 membered heteroaryl-C₁₋₄ alkyl are each optionally substituted with1, 2, 3, or 4 independently selected R^(8A) substituents; each R^(b8) isindependently selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl,phenyl, 4-7 membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl,which are each optionally substituted with 1, 2, 3, or 4 independentlyselected R^(8A) substituents; each R^(8A) is independently selected fromhalo, CN, NO₂, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄alkyl, phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, 5-6membered heteroaryl-C₁₋₄ alkyl, OR^(a81), SR^(a81), C(O)R^(b81),C(O)NR^(c81)R^(d81), C(O)OR^(a81), OC(O)R^(b81), OC(O)NR^(c81)R^(d81),NR^(c81)R^(d81), NR^(c81)C(O)R^(b81), NR^(c81)C(O)OR^(a81),NR^(c81)C(O)NR^(c81)R^(d81), NR^(c1)S(O)₂R^(b81),NR^(c81)S(O)₂NR^(c81)R^(d81), S(O)₂R^(b81), and S(O)₂NR^(c81)R^(d81),wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄alkyl, phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and5-6 membered heteroaryl-C₁₋₄ alkyl are each optionally substituted with1, 2, 3, or 4 independently selected R^(8B) substituents; each R^(a81),R^(c81), and R^(d81) is independently selected from H, C₁₋₆ alkyl, C₁₋₆haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄alkyl, wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl,4-7 membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(8B) substituents; each R^(b81) is independently selected from C₁₋₆alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl, which are each optionally substitutedwith 1, 2, 3, or 4 independently selected R^(8B) substituents; eachR^(8B) is independently selected from halo, CN, NO₂, C₁₋₆ alkyl, C₂₋₆alkenyl, C₂₋₆ alkynyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄alkyl, phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, 5-6membered heteroaryl-C₁₋₄ alkyl, OR^(a82), SR^(a82), C(O)R^(b82),C(O)NR^(c82)R^(b82), C(O)OR^(a82), OC(O)R^(b82), OC(O)NR^(c82)R^(d82),NR^(c82)R^(d82), NR^(c82)C(O)R^(b82), NR^(c82)C(O)OR^(a82),NR^(c82)C(O)NR^(c82)R^(d82), NR^(c82)S(O)₂R^(b82),NR^(c82)S(O)₂NR^(c82)R^(d82), S(O)₂R^(b82), and S(O)₂NR^(c82)R^(d82);each R^(a82), R^(c82), and R^(d82) is independently selected from H,C₁₋₆ alkyl, and C₁₋₆ haloalkyl; and each R^(b82) is independentlyselected from C₁₋₆ alkyl and C₁₋₆ haloalkyl.
 31. The compound of claim1, or a pharmaceutically acceptable salt thereof, wherein: n is 1 or 2;Z¹ is CR⁴R⁵; and Z² is O; or Z¹ is CR⁴R⁵; and Z² is CR^(4z)R^(5z); R⁴and R⁵ are each independently selected from H and C₁₋₃ alkyl; or R⁴ andR⁵, together with the carbon atom to which they are attached, form acyclopropyl or cyclobutyl ring; R^(4z) and R^(5z) are each H; Y is N; X¹is NR^(1x) or CR¹; X² is NR^(2x) or CR²; X³ is C; wherein Ring

is an aromatic ring selected from:

Ring moiety A is azetinidin-3-yl, pyrrolidin-3-yl, piperidin-3-yl, orpiperidin-4-yl; R¹ is selected from H, halo, C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl;wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄alkyl, phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and5-6 membered heteroaryl-C₁₋₄ alkyl are each optionally substituted by 1,2, or 3 independently selected R^(1A) substituents; R^(1x) is selectedfrom H, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄alkyl, phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and5-6 membered heteroaryl-C₁₋₄ alkyl; wherein said C₁₋₆ alkyl, C₁₋₆haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄alkyl are each optionally substituted by 1, 2, or 3 independentlyselected R^(1A) substituents; each R^(1A) is independently selected fromhalo, CN, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₄ cycloalkyl, OR^(a11), andNR^(c11)R^(d11); each R^(a11), R^(c11), and R^(d11) is independentlyselected from H and C₁₋₆ alkyl; R² is selected from H, halo, CN, C₁₋₆alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl; wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted by 1, 2, or 3 independently selected R^(2A)substituents; R^(2x) is selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl;wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄alkyl, phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and5-6 membered heteroaryl-C₁₋₄ alkyl are each optionally substituted by 1,2, or 3 independently selected R^(2A) substituents; each R^(2A) isindependently selected from halo, CN, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₄cycloalkyl, OR^(a21), and NR^(c21)R^(d21); each R^(a2), R^(c21), andR^(d21) is independently selected from H and C₁₋₆ alkyl; one R⁸ isS(O)₂R^(b8); the remaining R⁸ is selected from halo or C₁₋₃ alkyl; eachR^(b8) is independently selected from C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6 memberedheteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl,which are each optionally substituted with 1, 2, or 3 independentlyselected R^(8A) substituents; each R^(8A) is independently selected fromhalo, CN, NO₂, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄alkyl, phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, 5-6membered heteroaryl-C₁₋₄ alkyl, OR^(a81), SR^(a81), C(O)R^(b81),C(O)NR^(c81)R^(d81), C(O)OR^(a81), OC(O)R^(b81), OC(O)NR^(c81)R^(d81),NR^(c81)R^(d81), NR^(c81)C(O)R^(b81), NR^(c81)C(O)OR^(a81),NR^(c81)C(O)NR^(c81)R^(d81), NR^(c81)S(O)₂R^(b81),NR^(c81)S(O)₂NR^(c81)R^(d81), S(O)₂R^(b81), and S(O)₂NR^(c81)R^(d81),wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄alkyl, phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and5-6 membered heteroaryl-C₁₋₄ alkyl are each optionally substituted with1, 2, 3, or 4 independently selected R^(8B) substituents; each R^(a81),R^(c81), and R^(d81) is independently selected from H, C₁₋₆ alkyl, C₁₋₆haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 membered heterocycloalkyl, 5-6membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄alkyl, wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl,4-7 membered heterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇cycloalkyl-C₁₋₄ alkyl, phenyl-C₁₋₄ alkyl, 4-7 memberedheterocycloalkyl-C₁₋₄ alkyl, and 5-6 membered heteroaryl-C₁₋₄ alkyl areeach optionally substituted with 1, 2, 3, or 4 independently selectedR^(8B) substituents; each R^(b81) is independently selected from C₁₋₆alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, 5-6 membered heteroaryl, C₃₋₇ cycloalkyl-C₁₋₄ alkyl,phenyl-C₁₋₄ alkyl, 4-7 membered heterocycloalkyl-C₁₋₄ alkyl, and 5-6membered heteroaryl-C₁₋₄ alkyl, which are each optionally substitutedwith 1, 2, 3, or 4 independently selected R^(8B) substituents; eachR^(8B) is independently selected from halo, CN, C₁₋₆ alkyl, C₁₋₆haloalkyl, C₃₋₄ cycloalkyl, OR^(a82), SR^(a82), C(O)R^(b82),C(O)NR^(c82)R^(b82), C(O)OR^(a82), OC(O)R^(b82), OC(O)NR^(c82)R^(d82),NR^(c82)R^(d82), NR^(c82)C(O)R^(b82), NR^(c82)C(O)OR^(a82),R^(c82)C(O)NR^(c82)R^(d82), NR^(c82)S(O)₂R^(b82),NR^(c82)S(O)₂NR^(c82)R^(d82)S(O)₂R^(b82), and S(O)₂NR^(c82)R^(d82); eachR^(a82), R^(c82), and R^(d82) is independently selected from H, C₁₋₆alkyl, and C₁₋₆ haloalkyl; and each R^(b82) is independently selectedfrom C₁₋₆ alkyl and C₁₋₆ haloalkyl.
 32. The compound of any one ofclaims 1-2, or a pharmaceutically acceptable salt thereof, wherein: n is1 or 2; Z¹ is CR⁴R⁵; and Z² is O; or Z¹ is CR⁴R⁵; and Z² is CH₂; R⁴ andR⁵ are each independently selected from H and methyl; R^(4z) and R^(5z)are each H; Y is N; X¹ is NR^(1x) or CR¹; X² is NR^(2x) or CR²; X³ is C;wherein Ring

is an aromatic ring selected from:

wherein k is n−1; R¹ is selected from H, halo, C₁₋₆ alkyl, phenyl, and5-6 membered heteroaryl, wherein said C₁₋₆ alkyl, phenyl and 5-6membered heteroaryl are each optionally substituted by 1 or 2independently selected R^(1A) substituents; R^(1x) is selected from H,C₁₋₆ alkyl, C₃₋₇ cycloalkyl, phenyl, and 4-7 membered heterocycloalkyl,wherein said C₁₋₆ alkyl, C₃₋₇ cycloalkyl, phenyl, and 4-7 memberedheterocycloalkyl are each optionally substituted by 1 or 2 independentlyselected R^(1A) substituents; each R^(1A) is independently selected fromhalo, CN, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₃₋₄ cycloalkyl, OR^(a11), andNR^(c11)R^(d11); each R^(a11), R^(c11), and R^(d11) is independentlyselected from H and C₁₋₆ alkyl; R² is selected from H, halo, CN, C₁₋₆alkyl, C₁₋₆ haloalkyl, C₃₋₇ cycloalkyl, phenyl, 4-7 memberedheterocycloalkyl, and 5-6 membered heteroaryl; R^(2x) is selected fromH, C₁₋₆ alkyl, C₃₋₇ cycloalkyl, and phenyl-C₁₋₃ alkyl; R⁸ is C₁₋₃ alkyl;each R^(b8) is independently selected from C₁₋₆ alkyl, C₃₋₇ cycloalkyl,phenyl, 4-7 membered heterocycloalkyl, and 5-6 membered heteroaryl,which are each optionally substituted with 1 or 2 independently selectedR^(8A) substituents; each R^(8A) is independently selected from halo,CN, C₁₋₆ alkyl, C₁₋₆ haloalkyl, and 4-7 membered heterocycloalkyl;wherein said C₁₋₆ alkyl, C₁₋₆ haloalkyl, and 4-7 memberedheterocycloalkyl are each optionally substituted by 1 or 2 independentlyselected R^(8B) substituents; each R^(8B) is selected from C₁₋₆ alkyland OR^(a82); and each R^(a2) is independently selected from Hand C₁₋₆alkyl.
 33. The compound of any one of claims 1-32, having Formula (IVa):

or a pharmaceutically acceptable salt thereof, wherein k is n−1.
 34. Thecompound of any one of claims 1-32, having Formula (IVb):

or a pharmaceutically acceptable salt thereof, wherein k is n−1.
 35. Thecompound of any one of claims 1-32, having Formula (IVc):

or a pharmaceutically acceptable salt thereof, wherein k is n−1.
 36. Thecompound of any one of claims 1-32, having Formula (IVd):

or a pharmaceutically acceptable salt thereof, wherein k is n−1.
 37. Thecompound of claim 1, selected from:N-(1-(Ethylsulfonyl)piperidin-4-yl)-1,2-dimethyl-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine;N-(1-(Cyclopropylsulfonyl)piperidin-4-yl)-1,2-dimethyl-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine;1,2-Dimethyl-N-(1-((1-methyl-1H-pyrazol-4-yl)sulfonyl)piperidin-4-yl)-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine;N-(1-((1H-Pyrazol-4-yl)sulfonyl)piperidin-4-yl)-1,2-dimethyl-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine;3-((4-((1,2-Dimethyl-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-yl)amino)piperidin-1-yl)sulfonyl)benzonitrile;N-(1-((1,2-Dimethyl-1H-imidazol-4-yl)sulfonyl)piperidin-4-yl)-1,2-dimethyl-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine;2-Methyl-N-(1-(methylsulfonyl)piperidin-4-yl)-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine;1-Benzyl-2-methyl-N-(1-(methylsulfonyl)piperidin-4-yl)-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine;8-Methyl-N-(1-(methylsulfonyl)piperidin-4-yl)-6,8-dihydro-5H-pyrazolo[3,4-h]quinazolin-2-amine;8-Methyl-N-(1-(methylsulfonyl)piperidin-4-yl)-6,8-dihydropyrazolo[4′,3′:4,5]pyrano[3,2-d]pyrimidin-2-amine;8-(2,6-Difluorophenyl)-9-methyl-N-(1-(methylsulfonyl)piperidin-4-yl)-6,8-dihydro-5H-pyrazolo[3,4-h]quinazolin-2-amine;9-Cyclopropyl-8-methyl-N-(1-(methylsulfonyl)piperidin-4-yl)-6,8-dihydro-5H-pyrazolo[3,4-h]quinazolin-2-amine;9-Isopropyl-8-methyl-N-(1-(methylsulfonyl)piperidin-4-yl)-6,8-dihydro-5H-pyrazolo[3,4-h]quinazolin-2-amine;9-Cyclopropyl-N-(1-(methylsulfonyl)piperidin-4-yl)-8-(tetrahydrofuran-3-yl)-6,8-dihydro-5H-pyrazolo[3,4-h]quinazolin-2-amine;2-(9-Cyclopropyl-2-((1-(methylsulfonyl)piperidin-4-yl)amino)-5,6-dihydro-8H-pyrazolo[3,4-h]quinazolin-8-yl)ethan-1-ol;8,9-Dicyclopropyl-N-(1-(methylsulfonyl)piperidin-4-yl)-6,8-dihydro-5H-pyrazolo[3,4-h]quinazolin-2-amine;1,2-Dimethyl-N-(1-(methylsulfonyl)piperidin-4-yl)-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine;2-Isopropyl-1-methyl-N-(1-(methylsulfonyl)piperidin-4-yl)-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine;1-Cyclopropyl-2-methyl-N-(1-(methylsulfonyl)piperidin-4-yl)-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine;1-Cyclopropyl-N-(1-(methylsulfonyl)piperidin-4-yl)-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine;2-Bromo-1-cyclopropyl-N-(1-(methylsulfonyl)piperidin-4-yl)-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine;1-Cyclopropyl-N-(1-(methylsulfonyl)piperidin-4-yl)-2-phenyl-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine;1-Cyclopropyl-2-(2-fluorophenyl)-N-(1-(methylsulfonyl)piperidin-4-yl)-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine;1-Cyclopropyl-2-(1,3-dimethyl-1H-pyrazol-4-yl)-N-(1-(methylsulfonyl)piperidin-4-yl)-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine;1-Cyclopropyl-2,4,4-trimethyl-N-(1-(methylsulfonyl)piperidin-4-yl)-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine;and1-Cyclopropyl-N-(1-(methylsulfonyl)piperidin-4-yl)-1,4-dihydroimidazo[4′,5′:4,5]pyrano[3,2-d]pyrimidin-8-amine,or a pharmaceutically acceptable salt thereof.
 38. The compound of claim1, selected from:1-Isopropyl-2-methyl-N-(1-(methylsulfonyl)piperidin-4-yl)-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine;1,2-Dimethyl-N-((3R,4S)-3-methyl-1-((1-methyl-1H-imidazol-4-yl)sulfonyl)piperidin-4-yl)-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine;1,2-Dimethyl-N-((3R,4S)-3-methyl-1-((6-(4-methylpiperazin-1-yl)pyridin-2-yl)sulfonyl)piperidin-4-yl)-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine;(R)-1-(5-(((3R,4S)-4-((1,2-Dimethyl-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-yl)amino)-3-methylpiperidin-1-yl)sulfonyl)pyridin-2-yl)pyrrolidin-3-ol;1-Cyclopropyl-2-methyl-N-((3R,4S)-3-methyl-1-((1-methyl-1H-imidazol-4-yl)sulfonyl)piperidin-4-yl)-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine;and1-Cyclopropyl-2-methyl-N-((3R,4S)-3-methyl-1-((1-methyl-1H-pyrazol-4-yl)sulfonyl)piperidin-4-yl)-4,5-dihydro-1H-imidazo[4,5-h]quinazolin-8-amine,or a pharmaceutically acceptable salt thereof.
 39. A pharmaceuticalcomposition comprising a compound of any one of claims 1-38, or apharmaceutically acceptable salt thereof, and a pharmaceuticallyacceptable carrier.
 40. A method of inhibiting CDK2, comprisingcontacting the CDK2 with the compound of any one of claims 1-38, or apharmaceutically acceptable salt thereof.
 41. A method of inhibitingCDK2 in a patient, comprising administering to the patient the compoundof any one of claims 1-38, or a pharmaceutically acceptable saltthereof.
 42. A method of treating a disease or disorder associated withCDK2 in a patient, comprising administering to the patient atherapeutically effective amount of the compound of any one of claims1-38, or pharmaceutically acceptable salt thereof.
 43. A method oftreating a disease or disorder associated with CDK2 in a patient,comprising administering to the patient a therapeutically effectiveamount of the compound of any one of claims 1-38, or pharmaceuticallyacceptable salt thereof, wherein the disease or disorder is associatedwith an amplification of the cyclin E1 (CCNE1) gene and/oroverexpression of CCNE1.
 44. A method of treating a human subject havinga disease or disorder associated with cyclin-dependent kinase 2 (CDK2),comprising administering to the human subject a compound of any one ofclaims 1-38, or a pharmaceutically acceptable salt thereof, wherein thehuman subject has been previously determined to: (i) (a) have anucleotide sequence encoding a p16 protein comprising the amino acidsequence of SEQ ID NO:1; and/or (b) have a cyclin dependent kinaseinhibitor 2A (CDKN2A) gene lacking one or more inactivating nucleic acidsubstitutions and/or deletions; (ii) (a) have an amplification of thecyclin E1 (CCNE1) gene; and/or (b) have an expression level of CCNE1 ina biological sample obtained from the human subject that is higher thana control expression level of CCNE1.
 45. A method of treating a humansubject having a disease or disorder associated with cyclin-dependentkinase 2 (CDK2), comprising: (i) identifying, in a biological sampleobtained from the human subject: (a) a nucleotide sequence encoding ap16 protein comprising the amino acid sequence of SEQ ID NO:1; and/or(b) a cyclin dependent kinase inhibitor 2A (CDKN2A) gene lacking one ormore inactivating nucleic acid substitutions; (ii) identifying, in abiological sample obtained from the human subject: (a) an amplificationof the cyclin E1 (CCNE1) gene; and/or (b) an expression level of CCNE1that is higher than a control expression level of CCNE1; and (iii)administering a compound of any one of claims 1-38, or apharmaceutically acceptable salt thereof, to the human subject.
 46. Themethod of claim 45, comprising: (i) identifying, in a biological sampleobtained from the human subject: (a) a nucleotide sequence encoding ap16 protein comprising the amino acid sequence of SEQ ID NO:1; and/or(b) a CDKN2A gene lacking one or more inactivating nucleic acidsubstitutions and/or deletions; (ii) identifying, in a biological sampleobtained from the human subject: (a) an amplification of the CCNE1 gene;and (iii) administering the compound or the salt to the human subject.47. A method of evaluating the response of a human subject having adisease or disorder associated with cyclin-dependent kinase 2 (CDK2) toa compound of any one of claims 1-38, or a pharmaceutically acceptablesalt thereof, comprising: (a) administering the compound or the salt, tothe human subject, wherein the human subject has been previouslydetermined to have an amplification of the cyclin E1 (CCNE1) gene and/oran expression level of CCNE1 that is higher than a control expressionlevel of CCNE1; (b) measuring, in a biological sample of obtained fromthe subject subsequent to the administering of step (a), the level ofretinoblastoma (Rb) protein phosphorylation at the serine correspondingto amino acid position 780 of SEQ ID NO:3, wherein a reduced level of Rbphosphorylation at the serine corresponding to amino acid position 780of SEQ ID NO:3, as compared to a control level of Rb phosphorylation atthe serine corresponding to amino acid position 780 of SEQ ID NO:3, isindicative that the human subject responds to the compound or the salt.48. The method of any one of claims 42-47, wherein the disease ordisorder is cancer.